WO2004024926A3 - Transgene expressionskassetten zur expression von nukleinsäuren in kohlenhydrat-speichernden sink-geweben von pflanzen - Google Patents

Transgene expressionskassetten zur expression von nukleinsäuren in kohlenhydrat-speichernden sink-geweben von pflanzen Download PDF

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Publication number
WO2004024926A3
WO2004024926A3 PCT/EP2003/009855 EP0309855W WO2004024926A3 WO 2004024926 A3 WO2004024926 A3 WO 2004024926A3 EP 0309855 W EP0309855 W EP 0309855W WO 2004024926 A3 WO2004024926 A3 WO 2004024926A3
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WO
WIPO (PCT)
Prior art keywords
transgenic expression
plants
nucleic acid
acid sequences
expression cassettes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2003/009855
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English (en)
French (fr)
Other versions
WO2004024926A2 (de
Inventor
Ute Heim
Karin Herbers
Uwe Sonnewald
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SunGene GmbH
Original Assignee
SunGene GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SunGene GmbH filed Critical SunGene GmbH
Priority to US10/527,375 priority Critical patent/US7589254B2/en
Priority to CA2498294A priority patent/CA2498294C/en
Priority to AU2003271583A priority patent/AU2003271583B2/en
Priority to EP03753387A priority patent/EP1539967B1/de
Priority to DE50311379T priority patent/DE50311379D1/de
Publication of WO2004024926A2 publication Critical patent/WO2004024926A2/de
Publication of WO2004024926A3 publication Critical patent/WO2004024926A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8235—Fruit-specific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8226—Stem-specific, e.g. including tubers, beets
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)

Abstract

Die Erfindung betrifft Verfahren zur gezielten, transgenen Expression von Nukleinsäuresequenzen in den Kohlenhydrat-speichernden Sink-Geweben von Pflanzen unter Verwendung von transgenen Expressionskassetten, die den Promotoren der plastidären, 1,4-α-D-Glukanphosphat-α-D-glucosyltransferase aus Vicia faba enthalten. Weiterhin betrifft die Erfindung besagte transgene Expressionskassetten und diese enthaltende transgene Expressionsvektoren und transgene Organismen, sowie die Verwendung derselben zur Herstellung von Nahrungs-, Futtermitteln, Saatgut, Pharmazeutika oder Feinchemikalien.
PCT/EP2003/009855 2002-09-10 2003-09-05 Transgene expressionskassetten zur expression von nukleinsäuren in kohlenhydrat-speichernden sink-geweben von pflanzen Ceased WO2004024926A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/527,375 US7589254B2 (en) 2002-09-10 2003-09-05 Transgenic expression cassettes for expressing nucleic acid sequences in sink tissues of plants that store carbohydrate
CA2498294A CA2498294C (en) 2002-09-10 2003-09-05 Transgenic expression cassettes for expressing nucleic acid sequences in sink tissues of plants that store carbohydrate
AU2003271583A AU2003271583B2 (en) 2002-09-10 2003-09-05 Transgenic expression cassettes for expressing nucleic acid sequences in sink tissues of plants that store carbohydrate
EP03753387A EP1539967B1 (de) 2002-09-10 2003-09-05 Expressionskassetten zur expression von nukleinsäuren in kohlenhydrat-speichernden sink-geweben von pflanzen
DE50311379T DE50311379D1 (de) 2002-09-10 2003-09-05 Expressionskassetten zur expression von nukleinsäuren in kohlenhydrat-speichernden sink-geweben von pflanzen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10242204.4 2002-09-10
DE10242204 2002-09-10

Publications (2)

Publication Number Publication Date
WO2004024926A2 WO2004024926A2 (de) 2004-03-25
WO2004024926A3 true WO2004024926A3 (de) 2004-04-22

Family

ID=31983918

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/009855 Ceased WO2004024926A2 (de) 2002-09-10 2003-09-05 Transgene expressionskassetten zur expression von nukleinsäuren in kohlenhydrat-speichernden sink-geweben von pflanzen

Country Status (7)

Country Link
US (1) US7589254B2 (de)
EP (1) EP1539967B1 (de)
AT (1) ATE427358T1 (de)
AU (1) AU2003271583B2 (de)
CA (1) CA2498294C (de)
DE (1) DE50311379D1 (de)
WO (1) WO2004024926A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115976050B (zh) * 2022-11-16 2024-06-18 西北农林科技大学 猕猴桃感病基因及其适用的vigs沉默体系构建
CN120099047B (zh) * 2025-05-07 2025-08-12 江西省科学院生物资源研究所 一种提高果实即食性的phs基因和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998040503A1 (en) * 1997-03-10 1998-09-17 Planttec Biotechnologie Gmbh Nucleic acid molecules encoding starch phosphorylase from maize
WO2000026388A2 (de) * 1998-11-04 2000-05-11 Institut Für Pflanzengenetik Und Kulturpflanzenfor- Schung Neue expressionskassette zur expression von beliebigen genen in pflanzensamen

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3843627A1 (de) * 1988-12-21 1990-07-05 Inst Genbiologische Forschung Kartoffelknollenspezifische transkriptionale regulation
US5859330A (en) * 1989-12-12 1999-01-12 Epitope, Inc. Regulated expression of heterologous genes in plants and transgenic fruit with a modified ripening phenotype
ATE212670T1 (de) 1990-06-18 2002-02-15 Monsanto Technology Llc Erhöhter stärkegehalt in pflanzen
US5498830A (en) * 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5716837A (en) * 1995-02-10 1998-02-10 Monsanto Company Expression of sucrose phosphorylase in plants
CA2246242A1 (en) * 1996-02-08 1997-08-14 Institut Fur Pflanzengenetik Und Kulturpflanzenforschung Cassettes for the expression of storable proteins in plants
US6127179A (en) * 1996-04-17 2000-10-03 Dellapenna; Dean Gene promoter for tomato fruit
US6146875A (en) 1997-05-02 2000-11-14 Ward; N. Robert Method for culturing microorganisms in prefilled flexible containers
AU4492900A (en) 1999-04-30 2000-11-17 Agritope, Inc. Apple promoters for expression of transgenes in plants

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998040503A1 (en) * 1997-03-10 1998-09-17 Planttec Biotechnologie Gmbh Nucleic acid molecules encoding starch phosphorylase from maize
WO2000026388A2 (de) * 1998-11-04 2000-05-11 Institut Für Pflanzengenetik Und Kulturpflanzenfor- Schung Neue expressionskassette zur expression von beliebigen genen in pflanzensamen

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BRISSON N ET AL: "MATURATION AND SUBCELLULAR COMPARTMENTATION OF POTATO STARCH PHOSPHORYLASE", PLANT CELL, AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, US, vol. 1, no. 5, 1 May 1989 (1989-05-01), pages 559 - 566, XP000095141, ISSN: 1040-4651 *
BUCHNER P ET AL: "GLUCAN PHOSPHORYLASES IN VICIA FABA L.: CLONING, STRUCTURAL ANALYSIS AND EXPRESSION PATTERNS OF CYTOSOLIC AND PLASTIDIC FORMS IN RELATION TO STARCH", PLANTA, SPRINGER VERLAG, DE, vol. 199, 1996, pages 64 - 73, XP002071466, ISSN: 0032-0935 *
ST-PIERRE BENOIT ET AL: "5' deletion analysis of the potato starch phosphorylase gene: An upstream sequence defines distal regulatory elements and a proximal organ-dependent promoter", PLANT SCIENCE (LIMERICK), vol. 110, no. 2, 1995, pages 193 - 203, XP002270538, ISSN: 0168-9452 *
ST-PIERRE BENOIT ET AL: "The starch phosphorylase gene is subjected to different modes of regulation in starch-containing tissues of potato", PLANT MOLECULAR BIOLOGY, vol. 30, no. 6, 1996, pages 1087 - 1098, XP002270539, ISSN: 0167-4412 *

Also Published As

Publication number Publication date
EP1539967B1 (de) 2009-04-01
WO2004024926A2 (de) 2004-03-25
AU2003271583B2 (en) 2008-11-20
DE50311379D1 (de) 2009-05-14
US20050273888A1 (en) 2005-12-08
US7589254B2 (en) 2009-09-15
ATE427358T1 (de) 2009-04-15
EP1539967A2 (de) 2005-06-15
AU2003271583A1 (en) 2004-04-30
CA2498294A1 (en) 2004-03-25
CA2498294C (en) 2013-03-05

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