WO2004113553A3 - Procede de production d'amidites pures et d'oligonucleotides - Google Patents

Procede de production d'amidites pures et d'oligonucleotides Download PDF

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Publication number
WO2004113553A3
WO2004113553A3 PCT/US2004/018704 US2004018704W WO2004113553A3 WO 2004113553 A3 WO2004113553 A3 WO 2004113553A3 US 2004018704 W US2004018704 W US 2004018704W WO 2004113553 A3 WO2004113553 A3 WO 2004113553A3
Authority
WO
WIPO (PCT)
Prior art keywords
critical
oligonucleotides
amidites
producing pure
amidite
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/018704
Other languages
English (en)
Other versions
WO2004113553A8 (fr
WO2004113553A2 (fr
Inventor
Anthony N Scozzari
Achim H Krotz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ionis Pharmaceuticals Inc
Original Assignee
Isis Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Pharmaceuticals Inc filed Critical Isis Pharmaceuticals Inc
Publication of WO2004113553A2 publication Critical patent/WO2004113553A2/fr
Publication of WO2004113553A8 publication Critical patent/WO2004113553A8/fr
Anticipated expiration legal-status Critical
Publication of WO2004113553A3 publication Critical patent/WO2004113553A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Cette invention concerne la synthèse d'oligonucléotides. Cette invention concerne en particulier des procédés de production de phosphoramidites de haute qualité et d'oligonucléotides dérivés de ceux-ci. Dans certains modes de réalisation, ce procédé consiste à identifier et à mesurer un ou plusieurs signaux correspondant à une ou plusieurs impuretés critiques ou non critiques dans un échantillon d'amidite et à accepter ou à rejeter l'échantillon d'amidite selon que la quantité d'impureté critique ou non critique est inférieure ou égale à un seuil prédéterminé.
PCT/US2004/018704 2003-06-13 2004-06-14 Procede de production d'amidites pures et d'oligonucleotides Ceased WO2004113553A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US47839803P 2003-06-13 2003-06-13
US60/478,398 2003-06-13
US48870703P 2003-07-18 2003-07-18
US60/488,707 2003-07-18

Publications (3)

Publication Number Publication Date
WO2004113553A2 WO2004113553A2 (fr) 2004-12-29
WO2004113553A8 WO2004113553A8 (fr) 2005-06-23
WO2004113553A3 true WO2004113553A3 (fr) 2006-03-16

Family

ID=34681275

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/018704 Ceased WO2004113553A2 (fr) 2003-06-13 2004-06-14 Procede de production d'amidites pures et d'oligonucleotides

Country Status (1)

Country Link
WO (1) WO2004113553A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8084589B2 (en) 2007-08-31 2011-12-27 University Of Massachusetts Phosphoramidite nucleoside analogs
CN112409421A (zh) * 2020-12-01 2021-02-26 上海兆维科技发展有限公司 一种3’-磷酸酯核苷的制备方法
CN116413359B (zh) * 2023-03-08 2025-09-16 杭州诺泰诺和生物医药科技有限公司 一种亚磷酰胺单体液相色谱快速分析方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"DNA Phosphoramidites, Controlled Pore Glass and Columns", PROLIGO CATALOG., 2002, XP008047824 *

Also Published As

Publication number Publication date
WO2004113553A8 (fr) 2005-06-23
WO2004113553A2 (fr) 2004-12-29

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Free format text: IN PCT GAZETTE 53/2004 UNDER (30) ADD "60/488,707, 18 JULY 2003 (18.07.2003), US"

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