WO2005010156A3 - Genes codant des composes carotenoides - Google Patents

Genes codant des composes carotenoides Download PDF

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Publication number
WO2005010156A3
WO2005010156A3 PCT/US2004/022747 US2004022747W WO2005010156A3 WO 2005010156 A3 WO2005010156 A3 WO 2005010156A3 US 2004022747 W US2004022747 W US 2004022747W WO 2005010156 A3 WO2005010156 A3 WO 2005010156A3
Authority
WO
WIPO (PCT)
Prior art keywords
cluster
crtx
crtz
crty
crtb
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/022747
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English (en)
Other versions
WO2005010156A2 (fr
Inventor
Qiong Cheng
Natalia Sedkova
Luan Tao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Priority to CA002527557A priority Critical patent/CA2527557A1/fr
Priority to EP04778321A priority patent/EP1646713A2/fr
Priority to AU2004260061A priority patent/AU2004260061A1/en
Publication of WO2005010156A2 publication Critical patent/WO2005010156A2/fr
Anticipated expiration legal-status Critical
Priority to NO20060783A priority patent/NO20060783L/no
Publication of WO2005010156A3 publication Critical patent/WO2005010156A3/fr
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01029—Geranylgeranyl diphosphate synthase (2.5.1.29)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/03—Intramolecular oxidoreductases (5.3) transposing C=C bonds (5.3.3)
    • C12Y503/03002—Isopentenyl-diphosphate DELTA-isomerase (5.3.3.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Dans cette invention, une famille multigénique biosynthétique caroténogénique est isolée à partir de la souche DC413 de Panteoa stewartii, l'organisation génétique de cette famille multigénique correspondant à : crtE-idi-crtX-crtY-crtI-crtB-crtZ. Les gènes contenus dans la famille multigénique codent géranyl-géranyl pyrophosphate (GGPP) synthétase (CrtE), isopentényl pyrophosphate isomérase (Idi), zéaxanthine glucosyl transférase (CrtX), lycopène cyclase (CrtY), phytoène désaturase (CrtI), phytoène synthase (CrtB), et β-carotène hydroxylase (CrtZ). La famille multigénique, les gènes et leurs produits sont utilisés dans la conversion de la farnésyl-pyrophosphate en caroténoïdes. Par ailleurs, l'invention décrit les vecteurs contenant ces segments d'ADN, les cellules hôtes contenant ces vecteurs ainsi que les procédés de production de ces enzymes par la technologie de recombinaison de l'ADN dans des organismes hôtes transformés.
PCT/US2004/022747 2003-07-17 2004-07-14 Genes codant des composes carotenoides Ceased WO2005010156A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002527557A CA2527557A1 (fr) 2003-07-17 2004-07-14 Genes codant des composes carotenoides
EP04778321A EP1646713A2 (fr) 2003-07-17 2004-07-14 Genes codant des composes carotenoides
AU2004260061A AU2004260061A1 (en) 2003-07-17 2004-07-14 Genes encoding carotenoid compounds
NO20060783A NO20060783L (no) 2003-07-17 2006-02-17 Gener kodende for karotenoid forbindelser

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US48818303P 2003-07-17 2003-07-17
US60/488,183 2003-07-17
US52708303P 2003-12-03 2003-12-03
US60/527,083 2003-12-03

Publications (2)

Publication Number Publication Date
WO2005010156A2 WO2005010156A2 (fr) 2005-02-03
WO2005010156A3 true WO2005010156A3 (fr) 2007-05-03

Family

ID=34107764

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/022747 Ceased WO2005010156A2 (fr) 2003-07-17 2004-07-14 Genes codant des composes carotenoides

Country Status (4)

Country Link
EP (1) EP1646713A2 (fr)
AU (1) AU2004260061A1 (fr)
CA (1) CA2527557A1 (fr)
WO (1) WO2005010156A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1866428A2 (fr) 2005-03-18 2007-12-19 Microbia, Inc. Production de caroténoïdes dans de la levure ou des champignons oléagineux
CN111032855A (zh) * 2017-06-01 2020-04-17 尼普生物股份有限公司 微生物体中异源类胡萝卜素的产生
CN115807026B (zh) * 2022-08-01 2023-09-01 深圳大学 一种莱茵衣藻中虾青素合成路径的构建方法及应用
CN117264865B (zh) * 2023-11-21 2024-03-12 清华大学 重组红色红球菌及其在化合物合成中的应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MISAWA J. ET AL.: "Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeats", JOURNAL OF BIOTECHNOLOGY, vol. 59, 1998, pages 169 - 181, XP004113748 *
NGO ET AL.: "Computational Complexity;Protein Structure Prediction, and the Levinthal Paradox", THE PROTEIN FOLDING PROBLEM & TERTIARY STRUCTURE PREDICTION, 1994, pages 433 - 492-495 *
POTRYKUS I.: "Gene Transfer To Cereals: An Assessment", BIOTECHNOLOGY, vol. 8, no. 6, 1990, pages 535 - 542 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
US9297031B2 (en) 2006-09-28 2016-03-29 Dsm Ip Assets B.V. Production of carotenoids in oleaginous yeast and fungi

Also Published As

Publication number Publication date
EP1646713A2 (fr) 2006-04-19
CA2527557A1 (fr) 2005-02-03
WO2005010156A2 (fr) 2005-02-03
AU2004260061A1 (en) 2005-02-03

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