WO2006018205A3 - Neue essentielle gene von bacillus licheniformis und darauf aufbauende verbesserte biotechnologische produktionsverfahren - Google Patents

Neue essentielle gene von bacillus licheniformis und darauf aufbauende verbesserte biotechnologische produktionsverfahren Download PDF

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Publication number
WO2006018205A3
WO2006018205A3 PCT/EP2005/008683 EP2005008683W WO2006018205A3 WO 2006018205 A3 WO2006018205 A3 WO 2006018205A3 EP 2005008683 W EP2005008683 W EP 2005008683W WO 2006018205 A3 WO2006018205 A3 WO 2006018205A3
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WO
WIPO (PCT)
Prior art keywords
genes
bacillus licheniformis
essential genes
improved
production method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2005/008683
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English (en)
French (fr)
Other versions
WO2006018205A2 (de
Inventor
Joerg Feesche
Stefan Evers
Cornelius Bessler
Martina Plath
Armin Ehrenreich
Birgit Veith
Heiko Liesegang
Anke Henne
Christina Herzberg
Gerhard Gottschalk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henkel AG and Co KGaA
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Henkel AG and Co KGaA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henkel AG and Co KGaA filed Critical Henkel AG and Co KGaA
Publication of WO2006018205A2 publication Critical patent/WO2006018205A2/de
Publication of WO2006018205A3 publication Critical patent/WO2006018205A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Gegenstand der vorliegenden Anmeldung sind 150 neue essentielle Gene und deren Genprodukte von Bacillus licheniformis und hinreichend ähnliche Gene und Proteine. Diese Faktoren übernehmen in vivo jeweils elementare Lebensprozesse, beispielsweise die Replikation (zum Beispiel DNA-Polymerease, Helicase oder Gyrase), die Transkription (zum Beispiel RNA-Polymerase), die Proteinbiosynthese (ribosomale Proteine, Aminocyl-tRNA-Synthetasen, Initiations- und Elongationsfaktoren), die Sekretion von Proteinen (zum Beispiel Translocase) oder den Energie-Stoffwechsel. Ein Fehlen dieser Gene ist für die betreffenden Zellen unmittelbar letal und kann nicht durch eventuelle Verbindungen aus dem Nährmedium ausgeglichen werden. Somit können die zugehörigen Gene als Selektionsmarker genutzt werden. Dadurch lassen sich insofern verbesserte biotechnologische Produktionsverfahren durch Mikroorganismen etablieren, als eine Selektion über diese Gene ohne Antibiotika auskommt und zudem nicht durch Bestandteile üblicher Nährmedien ausgeglichen werden kann.
PCT/EP2005/008683 2004-08-19 2005-08-10 Neue essentielle gene von bacillus licheniformis und darauf aufbauende verbesserte biotechnologische produktionsverfahren Ceased WO2006018205A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004040134.9 2004-08-19
DE200410040134 DE102004040134A1 (de) 2004-08-19 2004-08-19 Neue essentielle Gene von Bacillus licheniformis und darauf aufbauende verbesserte biotechnologische Produktionsverfahren

Publications (2)

Publication Number Publication Date
WO2006018205A2 WO2006018205A2 (de) 2006-02-23
WO2006018205A3 true WO2006018205A3 (de) 2006-11-30

Family

ID=35721494

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/008683 Ceased WO2006018205A2 (de) 2004-08-19 2005-08-10 Neue essentielle gene von bacillus licheniformis und darauf aufbauende verbesserte biotechnologische produktionsverfahren

Country Status (2)

Country Link
DE (1) DE102004040134A1 (de)
WO (1) WO2006018205A2 (de)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US7960150B2 (en) 2007-01-25 2011-06-14 Danisco A/S Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
US7972638B2 (en) 1998-07-21 2011-07-05 Danisco A/S Foodstuff
US8030044B2 (en) 2003-12-24 2011-10-04 Danisco A/S Lipid acyltransferases
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006112738A1 (en) * 2005-04-19 2006-10-26 Auckland Uniservices Limited Novel peptides and methods for the treatment of inflammatory disorders
EP2099818A2 (de) * 2006-11-29 2009-09-16 Novozymes Inc. Bacillus licheniformis-chromosom
CN113969256A (zh) * 2020-07-24 2022-01-25 上海交通大学 一种生产n-乙酰氨基葡萄糖的菌株及其构建方法和应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0258118A1 (de) * 1986-08-05 1988-03-02 Transgene S.A. Verfahren zur Stabilisierung eines Plasmids, das in einem Bakterienstamm enthalten ist und der erhaltene Stamm
EP0972838A1 (de) * 1998-07-15 2000-01-19 Roche Diagnostics GmbH Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie
WO2004078953A1 (de) * 2003-03-04 2004-09-16 Henkel Kommanditgesellschaft Auf Aktien Ein translokationsenzym als selektionsmarker

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0258118A1 (de) * 1986-08-05 1988-03-02 Transgene S.A. Verfahren zur Stabilisierung eines Plasmids, das in einem Bakterienstamm enthalten ist und der erhaltene Stamm
EP0972838A1 (de) * 1998-07-15 2000-01-19 Roche Diagnostics GmbH Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie
WO2004078953A1 (de) * 2003-03-04 2004-09-16 Henkel Kommanditgesellschaft Auf Aktien Ein translokationsenzym als selektionsmarker

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"Bacillus licheniformis DSM 13, complete genome", EMBL, 21 September 2004 (2004-09-21), XP002339836 *
DATABASE EMBL [online] 2 July 1986 (1986-07-02), "Bacillus subtilis oriC region", XP002372816, retrieved from EBI accession no. EM_PRO:BSORIC Database accession no. BSORIC *
DATABASE EMBL [online] 6 February 2003 (2003-02-06), "Bacillus licheniformis rpoB gene for RNA polymerase beta subunit, partial cds, strain:IAM 11054.", XP002372818, retrieved from EBI accession no. EM_PRO:AB089342 Database accession no. AB089342 *
DATABASE Geneseq [online] 20 May 2004 (2004-05-20), "DNA polymerase III-type enzyme subunit #29.", XP002372817, retrieved from EBI accession no. GSN:ADK01158 Database accession no. ADK01158 *
DEGRYSE E: "Stability of a host-vector system based on complementation of an essential gene in Escherichia coli", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 18, no. 1/2, April 1991 (1991-04-01), pages 29 - 39, XP002092102, ISSN: 0168-1656 *
VEITH B ET AL: "THE COMPLETE GENOME SEQUENCE OF BACILLUS LICHENIFORMIS DSM13, AN ORGANISM WITH GREAT INDUSTRIAL POTENTIAL", JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, HORIZON SCIENTIFIC PRESS, WYMONDHAM,, GB, vol. 7, no. 4, 17 September 2004 (2004-09-17), pages 204 - 211, XP009047713, ISSN: 1464-1801 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7972638B2 (en) 1998-07-21 2011-07-05 Danisco A/S Foodstuff
US8163315B2 (en) 1998-07-21 2012-04-24 Danisco A/S Foodstuff
USRE43135E1 (en) 2001-05-18 2012-01-24 Danisco A/S Method of improving dough and bread quality
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US8030044B2 (en) 2003-12-24 2011-10-04 Danisco A/S Lipid acyltransferases
US7960150B2 (en) 2007-01-25 2011-06-14 Danisco A/S Production of a lipid acyltransferase from transformed Bacillus licheniformis cells

Also Published As

Publication number Publication date
DE102004040134A1 (de) 2006-02-23
WO2006018205A2 (de) 2006-02-23

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