WO2006040104A2 - Sequençage d'acides nucleiques par deplacement de brin - Google Patents

Sequençage d'acides nucleiques par deplacement de brin Download PDF

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Publication number
WO2006040104A2
WO2006040104A2 PCT/EP2005/010869 EP2005010869W WO2006040104A2 WO 2006040104 A2 WO2006040104 A2 WO 2006040104A2 EP 2005010869 W EP2005010869 W EP 2005010869W WO 2006040104 A2 WO2006040104 A2 WO 2006040104A2
Authority
WO
WIPO (PCT)
Prior art keywords
sequencing
nucleic acid
strand displacement
nucleic acids
strand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2005/010869
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German (de)
English (en)
Other versions
WO2006040104A3 (fr
Inventor
Achim Fischer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2006040104A2 publication Critical patent/WO2006040104A2/fr
Publication of WO2006040104A3 publication Critical patent/WO2006040104A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de séquençage d'acides nucléiques, consistant (a) à préparer au moins un acide nucléique à séquencer sous forme immobilisée et au moins partiellement bicaténaire, (b) à hybrider une amorce de séquençage sur un des deux brins de l'acide nucléique et (c) à séquencer au moins une section de l'acide nucléique par extension de l'amorce de séquençage et par déplacement du brin d'acide nucléique qui était présent sous forme hybridée sur le brin auquel l'amorce est fixée.
PCT/EP2005/010869 2004-10-09 2005-10-08 Sequençage d'acides nucleiques par deplacement de brin Ceased WO2006040104A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200410049532 DE102004049532A1 (de) 2004-10-09 2004-10-09 Sequenzierung von Nukleinsäuren über Strand Displacement
DE102004049532.7 2004-10-09

Publications (2)

Publication Number Publication Date
WO2006040104A2 true WO2006040104A2 (fr) 2006-04-20
WO2006040104A3 WO2006040104A3 (fr) 2006-07-20

Family

ID=35355917

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/010869 Ceased WO2006040104A2 (fr) 2004-10-09 2005-10-08 Sequençage d'acides nucleiques par deplacement de brin

Country Status (2)

Country Link
DE (1) DE102004049532A1 (fr)
WO (1) WO2006040104A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2453023A3 (fr) * 2006-10-06 2012-08-22 Illumina Cambridge Limited Procédé de séquençage d'un modèle de polynucléotide

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US6436635B1 (en) * 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
CA2122203C (fr) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination des reactions d'amplification d'acides nucleiques
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US6258533B1 (en) * 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US5981190A (en) * 1997-01-08 1999-11-09 Ontogeny, Inc. Analysis of gene expression, methods and reagents therefor
WO2001075154A2 (fr) * 2000-04-03 2001-10-11 Axaron Bioscience Ag Procede de sequençage parallele d'un melange d'acides nucleiques sur une surface

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2453023A3 (fr) * 2006-10-06 2012-08-22 Illumina Cambridge Limited Procédé de séquençage d'un modèle de polynucléotide
US8431348B2 (en) 2006-10-06 2013-04-30 Illumina Cambridge Limited Method for pairwise sequencing of target polynucleotides
US8765381B2 (en) 2006-10-06 2014-07-01 Illumina Cambridge Limited Method for pairwise sequencing of target polynucleotides
US9267173B2 (en) 2006-10-06 2016-02-23 Illumina Cambridge Limited Method for pairwise sequencing of target polynucleotides
US10221452B2 (en) 2006-10-06 2019-03-05 Illumina Cambridge Limited Method for pairwise sequencing of target polynucleotides
EP3670672A1 (fr) * 2006-10-06 2020-06-24 Illumina Cambridge Limited Procédé de séquençage d'un modèle de polynucléotide

Also Published As

Publication number Publication date
DE102004049532A1 (de) 2006-04-13
WO2006040104A3 (fr) 2006-07-20

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