WO2006040104A3 - Sequenzierung von nukleinsäuren unter strand displacement - Google Patents

Sequenzierung von nukleinsäuren unter strand displacement Download PDF

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Publication number
WO2006040104A3
WO2006040104A3 PCT/EP2005/010869 EP2005010869W WO2006040104A3 WO 2006040104 A3 WO2006040104 A3 WO 2006040104A3 EP 2005010869 W EP2005010869 W EP 2005010869W WO 2006040104 A3 WO2006040104 A3 WO 2006040104A3
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WO
WIPO (PCT)
Prior art keywords
sequencing
nucleic acid
strand displacement
nucleic acids
strand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2005/010869
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English (en)
French (fr)
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WO2006040104A2 (de
Inventor
Achim Fischer
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Individual
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Individual
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Publication date
Application filed by Individual filed Critical Individual
Publication of WO2006040104A2 publication Critical patent/WO2006040104A2/de
Publication of WO2006040104A3 publication Critical patent/WO2006040104A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Gegenstand der Erfindung ist ein Verfahren zur Nukleinsäuresequenzierung, umfassend die Schritte: (a) Bereitstellen mindestens einer zu sequenzierenden Nukleinsäure in immobilisierter und mindestens teilweise doppelsträngiger Form, (b) Hybridisierung eines Sequenzierprimers an einen der beiden Stränge der Nukleinsäure, (c) Sequenzierung mindestens eines Abschnitts der Nukleinsäure unter Verlängerung des Sequenzierprimers und unter strand displacement desjenigen Nukleinsäurestrangs, welcher an den primerbindenden Strang hybridisiert vorlag.
PCT/EP2005/010869 2004-10-09 2005-10-08 Sequenzierung von nukleinsäuren unter strand displacement Ceased WO2006040104A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200410049532 DE102004049532A1 (de) 2004-10-09 2004-10-09 Sequenzierung von Nukleinsäuren über Strand Displacement
DE102004049532.7 2004-10-09

Publications (2)

Publication Number Publication Date
WO2006040104A2 WO2006040104A2 (de) 2006-04-20
WO2006040104A3 true WO2006040104A3 (de) 2006-07-20

Family

ID=35355917

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/010869 Ceased WO2006040104A2 (de) 2004-10-09 2005-10-08 Sequenzierung von nukleinsäuren unter strand displacement

Country Status (2)

Country Link
DE (1) DE102004049532A1 (de)
WO (1) WO2006040104A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0497272A1 (de) * 1991-01-31 1992-08-05 Becton, Dickinson and Company Strangverdrängungsamplifikation
EP0624643A2 (de) * 1993-05-11 1994-11-17 Becton, Dickinson and Company Dekontamination von Nukleinsäure-Amplifikationsreaktionen
EP0684315A1 (de) * 1994-04-18 1995-11-29 Becton, Dickinson and Company Strangverdrängungsamplifikation unter Verwendung von thermophilen Enzymen
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
WO1999045153A2 (en) * 1998-03-05 1999-09-10 The University Of Iowa Research Foundation An iterative and regenerative dna sequencing method
US5981190A (en) * 1997-01-08 1999-11-09 Ontogeny, Inc. Analysis of gene expression, methods and reagents therefor
WO2001075154A2 (de) * 2000-04-03 2001-10-11 Axaron Bioscience Ag Neue verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche
US6436635B1 (en) * 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0497272A1 (de) * 1991-01-31 1992-08-05 Becton, Dickinson and Company Strangverdrängungsamplifikation
US6436635B1 (en) * 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
EP0624643A2 (de) * 1993-05-11 1994-11-17 Becton, Dickinson and Company Dekontamination von Nukleinsäure-Amplifikationsreaktionen
US5536649A (en) * 1993-05-11 1996-07-16 Becton, Dickinson And Company Decontamination of nucleic acid amplification reactions using uracil-N-glycosylase (UDG)
EP0684315A1 (de) * 1994-04-18 1995-11-29 Becton, Dickinson and Company Strangverdrängungsamplifikation unter Verwendung von thermophilen Enzymen
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US5981190A (en) * 1997-01-08 1999-11-09 Ontogeny, Inc. Analysis of gene expression, methods and reagents therefor
WO1999045153A2 (en) * 1998-03-05 1999-09-10 The University Of Iowa Research Foundation An iterative and regenerative dna sequencing method
WO2001075154A2 (de) * 2000-04-03 2001-10-11 Axaron Bioscience Ag Neue verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DUNN JOHN J ET AL: "Genomic signature tags (GSTs): A system for profiling genomic DNA", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, WOODBURY, NY, US, vol. 12, no. 11, November 2002 (2002-11-01), pages 1756 - 1765, XP002304617, ISSN: 1088-9051 *

Also Published As

Publication number Publication date
DE102004049532A1 (de) 2006-04-13
WO2006040104A2 (de) 2006-04-20

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