WO2006079020A3 - Identification fonctionnelle du gene de muticus hyoscyamus codant pour l'activite de la premnaspirodiene hydroxylase - Google Patents

Identification fonctionnelle du gene de muticus hyoscyamus codant pour l'activite de la premnaspirodiene hydroxylase Download PDF

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Publication number
WO2006079020A3
WO2006079020A3 PCT/US2006/002265 US2006002265W WO2006079020A3 WO 2006079020 A3 WO2006079020 A3 WO 2006079020A3 US 2006002265 W US2006002265 W US 2006002265W WO 2006079020 A3 WO2006079020 A3 WO 2006079020A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
premnaspirodiene
gene coding
hyoscyamus muticus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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PCT/US2006/002265
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English (en)
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WO2006079020A2 (fr
Inventor
Joe Chappell
Yunsoo Yeo
Shunji Takahashji
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University of Kentucky Research Foundation
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University of Kentucky Research Foundation
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Publication date
Application filed by University of Kentucky Research Foundation filed Critical University of Kentucky Research Foundation
Publication of WO2006079020A2 publication Critical patent/WO2006079020A2/fr
Anticipated expiration legal-status Critical
Publication of WO2006079020A3 publication Critical patent/WO2006079020A3/fr
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des molécules d'acide nucléique isolées et purifiées codant pour la premnaspirodiène oxydase de Hyoscyamus muticus (HPO), préparées par clonage moléculaire. Ces molécules d'acide nucléique codent pour l'enzyme oxydase qui peut catalyser l'hydroxylation de la valencène en bêta-nootkatol et en nootkatone, présentant un intérêt sur le plan industriel et utilisé dans les produits alimentaires. L'invention concerne également des vecteurs renfermant ces molécules d'acide nucléique, des cellules hôtes transformées ou transfectées par les vecteurs, ainsi que des méthodes destinées à la production de protéines par expression des molécules d'acide nucléique dans les cellules hôtes. L'invention concerne également des méthodes destinées à la production d'un substrat de terpène oxydé à partir d'un substrat de terpène non oxydé, dans lesquelles sont utilisées des protéines ou des molécules d'acide nucléique exprimées selon l'invention.
PCT/US2006/002265 2005-01-19 2006-01-19 Identification fonctionnelle du gene de muticus hyoscyamus codant pour l'activite de la premnaspirodiene hydroxylase Ceased WO2006079020A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64530405P 2005-01-19 2005-01-19
US60/645,304 2005-01-19

Publications (2)

Publication Number Publication Date
WO2006079020A2 WO2006079020A2 (fr) 2006-07-27
WO2006079020A3 true WO2006079020A3 (fr) 2008-12-04

Family

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PCT/US2006/002265 Ceased WO2006079020A2 (fr) 2005-01-19 2006-01-19 Identification fonctionnelle du gene de muticus hyoscyamus codant pour l'activite de la premnaspirodiene hydroxylase

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WO (1) WO2006079020A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8106260B2 (en) 1996-04-12 2012-01-31 The Board Of Trustees Of The University Of Kentucky Chimeric isoprenoid synthases and uses thereof
US7442785B2 (en) 2003-07-24 2008-10-28 The University Of Kentucky Research Foundation Sesquiterpene synthase gene and protein
EP3162787A1 (fr) 2007-03-20 2017-05-03 Evolva, Inc. Compositions comprenant du 5-epi-beta-vetivone et son utilisation comme parfum
US8124811B2 (en) 2007-03-20 2012-02-28 Allylix, Inc. Fragrance and methods for production of 5-epi-β-vetivone, 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one, and 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-1,6-dien-8-one
AU2012204126B2 (en) * 2007-03-20 2014-05-15 Evolva, Inc. Novel Methods for Production of 5-epi-beta-vetivone, 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one, and 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-1,6-dien-8-one
EP2336310A1 (fr) 2009-12-16 2011-06-22 Isobionics B.V. Valencène synthase
CN103282492A (zh) * 2010-10-29 2013-09-04 奥利里克斯股份有限公司 修饰的瓦伦烯合酶多肽、编码核酸分子及其用途
EP2537926A1 (fr) 2011-06-21 2012-12-26 Isobionics B.V. Valencène synthase
WO2014099821A2 (fr) 2012-12-18 2014-06-26 Allylix, Inc. Solavétivone et 5-épi-bêta-vétivone en tant qu'agents répulsifs de nuisibles et pesticides
US10501760B2 (en) 2014-08-21 2019-12-10 Givaudan Sa Methods for production of oxygenated terpenes
EP3377642A1 (fr) * 2015-11-16 2018-09-26 Evolva SA Production de nootkatol glycosylé dans des hôtes de recombinaison
CN116103249A (zh) * 2021-11-09 2023-05-12 华南理工大学 瓦伦西亚烯氧化酶突变体及其在制备圆柚酮中的应用
CN114480512B (zh) 2021-12-13 2022-10-25 华南理工大学 氧化还原酶及其突变体在生物合成圆柚酮中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050233336A1 (en) * 2004-04-19 2005-10-20 O'maille Paul E Compositions and methods for producing libraries with controlled compositions and screening probabilities
US20060218661A1 (en) * 2003-07-24 2006-09-28 Joe Chappell Novel sesquiterpene synthase gene and protein

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060218661A1 (en) * 2003-07-24 2006-09-28 Joe Chappell Novel sesquiterpene synthase gene and protein
US20050233336A1 (en) * 2004-04-19 2005-10-20 O'maille Paul E Compositions and methods for producing libraries with controlled compositions and screening probabilities

Also Published As

Publication number Publication date
WO2006079020A2 (fr) 2006-07-27

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