WO2006090902A1 - Composition pour le controle d'organismes nocifs - Google Patents

Composition pour le controle d'organismes nocifs Download PDF

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Publication number
WO2006090902A1
WO2006090902A1 PCT/JP2006/303929 JP2006303929W WO2006090902A1 WO 2006090902 A1 WO2006090902 A1 WO 2006090902A1 JP 2006303929 W JP2006303929 W JP 2006303929W WO 2006090902 A1 WO2006090902 A1 WO 2006090902A1
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WIPO (PCT)
Prior art keywords
protein
pest control
polyethylene glycol
produced
bacillus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2006/303929
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English (en)
Japanese (ja)
Inventor
Shinji Isayama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sumitomo Chemical Co Ltd
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Sumitomo Chemical Co Ltd
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Application filed by Sumitomo Chemical Co Ltd filed Critical Sumitomo Chemical Co Ltd
Publication of WO2006090902A1 publication Critical patent/WO2006090902A1/fr
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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis

Definitions

  • the present invention relates to a pest control composition, and more specifically to a pest control composition containing an insecticidal protein that can be produced by Bacillus thuringiesis and polyethylene glycol. Background art
  • insecticidal protein of Bacillus thuringiesis has insecticidal activity (for example, see Toshihiko Fukuhara (Institute Publication Center) 01641-1104).
  • the present invention relates to a combination in a pest control composition that can obtain a synergistic effect that is more than the additive effect that is expected to be obtained when it is simply mixed, and that can reduce the amount used.
  • composition of the present invention Contains an insecticidal protein that may be produced by Bacillus thuringiesis (hereinafter sometimes referred to as this protein) and polyethylene glycol having a molecular weight in the range of 2,000 to 800,000 A pest control composition characterized by the following (hereinafter also referred to as the composition of the present invention);
  • Insecticidal proteins that can be produced by Bacillus thuringiesis and polyethylene glycols with molecular weights ranging from 2,000 to 800,000
  • a pest control composition characterized by containing
  • the preceding paragraph 1 or 2 characterized in that the insecticidal protein that can be produced by Bacillus turingiesis and polyethylene glycol are contained in a weight ratio of 1: 1000 to 10: 1. 4.
  • the insecticidal protein is a live cell or spore of Bacillus thuringies is, a cell that has been killed by heat or chemical means, or 3.
  • Effective amounts of both insecticidal protein and polyethylene glycol that can be produced by Bacillus thuringiesis can be mixed or simultaneously or non-simultaneously used to protect plants, pests or harmful Pest control method characterized by being applied to the habitat of the organism;
  • Effective amounts of both insecticidal protein and polyethylene glycol which can be produced by Bacillus thuringiesis, can be mixed or used simultaneously or in combination to protect plants, pests or pests to be protected.
  • a pest control method characterized by being applied to the habitat;
  • the present protein used as one active ingredient in the composition of the present invention is not particularly limited as long as it is an insecticidal protein derived from Bacillus thuringiesis.
  • Specific examples of the protein include C rylA (a), C rylA (b), C rylA (c), CryIB, CryIC, CryID, CryL F, CryIIA, CryIIB, CryV, Cry VI, etc. can be mentioned.
  • These proteins are naturally produced by bacteria belonging to Bacillus thuringiesis.
  • an insecticidal protein produced by the bacterium belonging to a subspecies such as kurstaki and aizawai can be preferably used in the composition of the present invention.
  • this protein is an insecticidal protein produced by a bacterium belonging to Bacillus thuringiesis that exists in nature, it is a trait produced using ordinary genetic engineering techniques. It may be an insecticidal protein produced by a converter (eg, E. coli, Bacillus subtilis, plant, etc.).
  • a gene having a base sequence encoding the amino acid sequence of the present protein (hereinafter sometimes referred to as the present gene), which is used in producing such a transformant, is (1) naturally present.
  • Such microorganisms may be isolated from nature or purchased from strain preservation institutions. Further, it may be artificially prepared from such a microorganism in accordance with a normal microbiological technique or genetic engineering technique.
  • the protein used in the composition of the present invention may be an isolated protein, for example, a live cell or spore of Bacillus thuringiesis, which is killed by heat or chemical means. It may be an insecticidal protein that is in a state of being included in a fungus body that has been bacterized or a debris of the fungus body.
  • Examples of the medium for cultivating bacteria belonging to Bacillus thuringiesis that produce insecticidal proteins include general bacterial media and normal broth liquid medium. Any medium can be used. As described above, the bacteria belonging to Bacillus thuringiesis and its product cultured using the above medium are centrifuged and Z or dried together with the medium as one active ingredient of the composition of the present invention. It can be used as a certain present protein. Alternatively, the insecticidal protein itself separated from the medium according to a known method may be used as the protein as it is. Incidentally, the molecular weight of the protein itself is tens of thousands to hundreds of thousands of daltons.
  • the protein used in the composition of the present invention may be a commercially available BT preparation, preferably Dipole (registered trademark) (Sumitomo Chemical Co., Ltd.), Esmarck (registered trademark) (Sumitomo Chemical ( ), Flowback (registered trademark) (Sumitomo Chemical Co., Ltd.), Zenyuri (registered trademark) (Sumika Takeda Agrochemical Co., Ltd.), and the like.
  • Polyethylene glycol having a molecular weight in the range of 2,000 to 800,000 (hereinafter sometimes referred to as the present compound) used as another active ingredient in the composition of the present invention is [_CH 2 CH 2 O—]
  • a polymer of ethylene oxide represented by n also called ethylene oxide, polyoxyethylene, and its molecular weight ranges from 2,000 to 800,000.
  • the preferred molecular weight can be in the range of 4,000 to 500,000.
  • composition of the present invention can be suitably used, for example, for controlling Lepidoptera pests in a wide range of agricultural and horticulture. Typical examples include the following.
  • Chilo suppressalis Cnap alocrocis medinalis, 3 bite Piancorn holer (Ostrinia nubilalis), Parapediasia te terrella, etc., Spodoptera pod, Pseudaletia separata;, Ma mestra brasicae, AgroUs ipsilon, Trich oplusia ni, Autographa nigrisigna, Heliothis sp. He Heliothis sp. Helicovei ⁇ pa spp., Earias sp p.
  • the mixing ratio of the present protein and the present compound may be any mixing ratio that produces a synergistic effect on the pesticidal activity, for example, a ratio of 1: 100 to 10: 1 by weight.
  • the ratio is preferably 1: 500 to 5: 1, more preferably the ratio 1: 5 to 2: 1.
  • the present compound and the protein may be used as they are without adding any other components.
  • the mixture is further added to a solid carrier, liquid carrier or
  • a surfactant such as a gaseous carrier, if necessary, other formulation adjuvants, oils, emulsions, wettable powders, granulated wettable powders, suspensions in water and emulsions in water
  • a surfactant such as a gaseous carrier
  • other formulation adjuvants such as a gaseous carrier
  • other formulation adjuvants such as a gaseous carrier
  • wettable powders emulsions
  • wettable powders emulated wettable powders
  • emulsions emulsions in water
  • the composition of the present invention is usually contained in an amount of about 0.01 to 95% by weight as an active ingredient.
  • solid carriers used for formulation examples include clays (kaolin clay, diatomaceous earth, bentonite, fusami clay, acid clay), synthetic silicon hydroxide, talc, ceramics, and other inorganic minerals ( Fine powders such as sericite, quartz, sulfur, activated carbon, calcium carbonate, hydrated silica, etc.), chemical fertilizers (ammonium sulfate, phosphorous acid, ammonium nitrate, urea, ammonium chloride, etc.) Examples include granular materials.
  • liquid carriers examples include water, alcohols (methanol, ethanol, etc.), ketones (acetone, methyl ethyl ketone, etc.), aromatic hydrocarbons (toluene, xylene, ethylbenzene, methyl naphthalene, etc.), Non-aromatic hydrocarbons (hexane, cyclohexane, kerosene, light oil, etc.), esters (ethyl acetate, butyl acetate, etc.), nitriles (acetonitrile, isoptyronitrile, etc.), ethers (diisopropyl ether) ), Acid amides (N, N-dimethylformamide, N, N-dimethylacetamides), halogenated hydrocarbons (dichloromethane, trichloroethane, carbon tetrachloride, etc.), dimethyl sulfoxide, vegetable oil (soybean oil) , Cottonseed
  • gaseous carrier that is, the propellant
  • propellant examples include, for example, Freon gas, butane gas, liquefied petroleum gas, dimethyl ether, carbon dioxide gas and the like.
  • surfactant examples include alkyl sulfate ester salts, alkyl sulfonates, alkyl aryl sulfonates, alkyl aryl ethers, polyhydric alcohol esters, sugar alcohol derivatives and the like.
  • adjuvants for formulation include, for example, casein, gelatin, saccharides (starch, arabia gum, cellulose derivatives, alginic acid, etc.), lignin derivatives, bentonite, synthetic water-soluble polymers (polyvinyl alcohol, polyvinyl pyrrolidone, poly Acrylic acid, etc.), PAP (isopropyl acid phosphate), BHT (2,6-di-tert-butyl-4-methylphenol), BHA (2-tert-butyl-4-methoxyphenol) and 3-ter t -Petitlou 4-methoxyphenol), vegetable oils, mineral oils, surfactants, fatty acids, fatty acid esters and the like.
  • bases for poisonous baits include grain ingredients, vegetable oils, sugars, crystalline components such as crystalline cellulose, antioxidants such as dibutylhydroxytoluene and nordihydroguaaretic acid, preservatives such as dehydroacetic acid, and pepper powder.
  • Accident prevention agent such as cheese flavor, onion flavor, etc.
  • Attractive fragrances A flowable agent such as a suspension in water or an emulsion in water usually contains about 1 to 75% by weight of an active ingredient compound and about 0.5 to 15% by weight of a suspension aid (for example, protective colloidal thixotropy).
  • compositions capable of imparting a certain nature about 0 to 10% by weight of auxiliary agents (for example, antifoaming agents, antifungal agents, stabilizers, spreading agents, penetration aids, antifreezing agents, antiseptics, antifungal agents) Etc.) can be obtained by slightly dispersing in water.
  • auxiliary agents for example, antifoaming agents, antifungal agents, stabilizers, spreading agents, penetration aids, antifreezing agents, antiseptics, antifungal agents
  • Etc. can be obtained by slightly dispersing in water.
  • the composition of the present invention can also be prepared by formulating each active ingredient by the above-described formulation method and then mixing these formulations. That is, the composition of the present invention may be prepared by mixing a pre-formulated compound of the present compound and a pre-formulated protein of the present compound, depending on the preparation form. Moreover, both can be mixed at the time of application, or simultaneously or non-simultaneously.
  • composition of the present invention thus formulated is used as it is or diluted in water or the like.
  • other insecticides, acaricides, nematicides, fungicides, herbicides, plant growth regulators, synergists, fertilizers, soil conditioners, animal feeds, etc. can also be used together.
  • the composition of the present invention is treated with an effective amount of pests, pest habitats, plants to be protected from pests, and the like. At that time, when the composition of the present invention is formulated into an emulsion, a wettable powder, a granular wettable powder, a flowable powder, etc., it is usually diluted with water.
  • the effective amount or application amount of the present composition is generally about at 1 0 0 0 m 2 application rate per 0. 1 to 1 0 0 0 g.
  • active ingredients for controlling pests in the form of granules, powders, oils, etc.
  • the active ingredient for controlling pests is used after diluting an emulsion, wettable powder, granular wettable powder, flowable powder, etc. in water, the above effective amount or What is necessary is just to dilute with water so that it may become an application rate.
  • the application concentration of the composition of the present invention is usually about 1 to 10,000 ppm. I can give you.
  • the above-mentioned effective amount or application amount varies depending on the type of preparation, application time, application location, application method, pest type, damage level, etc., and can be selected as appropriate without changing within the above range. be able to.
  • the “BT fungal culture” described later refers to a spore suspension 100 1 containing 1 ⁇ 10 3 viable spores per 1 m 1 of bacteria belonging to Bacillus thuringiesis. Inoculate a sterilized L broth liquid medium (100 ml) in a 500 ml flask and perform shaking culture (150 rpm) for about 2 to 5 days under 25 conditions. A culture obtained by centrifuging and drying both spores and insecticidal proteins from bacteria belonging to Bacillus thuringiesis (ie, one form of the protein) ) Means.
  • This compound (Molecular weights 4,000, 20,000 and 500,000) 0.5 part, 4 parts of BT bacterial culture, 1 part of synthetic silicon hydroxide fine powder, 1 part of Doreles B (manufactured by Sankyo) as flocculant Mix well 7 parts of clay with a mortar and then stir and mix with a juice mixer. Add 86.5 parts of the cutlet to the resulting mixture and mix thoroughly to obtain a powder.
  • xanthan gum and 1.0 part of bee gum R (aluminum magnesium silicate manufactured by Sanyo Chemical Co., Ltd.) are dispersed in 56.3 parts of ion-exchanged water to obtain a thickener solution.
  • the composition of the present invention exhibits an excellent pest control effect.
  • BT fungus preparation Flowback (trademark registered) (Bacillus thuringiensis var. Aizaw ai) Dry flowable preparation, manufactured by Sumitomo Chemical Co., Ltd.) and polyethylene glycol (hereinafter referred to as PEG; manufactured by Wako Pure Chemical Industries, Ltd.) Mix with the diluent to adjust each A test chemical solution was prepared by adding a 1Z5000 amount of the diluting solution (special Rinoichi: manufactured by Nippon Agricultural Chemicals Co., Ltd.) to the diluting solution added so as to be suitable.
  • PEG PEGs having molecular weights of 400, 1,000, 4,000, 20,000, 500, 000 and 1,000,000 were used.
  • Reagent reagent Application concentration Insecticidal rate Synergistic effect of insecticidal rate Theoretical value (%) Actual value (%) Undetermined result This protein 100 20. 0
  • Test example 2 Food leaf immersion test for Spodoptera litura, examination of optimum mixing amount
  • BT bacteria preparation Flowback (registered trademark) (Bacillus thuringiensis var. Aizaw ai) Dry flowable preparation, manufactured by Sumitomo Chemical Co., Ltd. and polyethylene glycol (this compound, manufactured by Wako Pure Chemical Industries, Ltd.) Add the spreading agent (special Rinoichi: manufactured by Nippon Agricultural Chemicals Co., Ltd.) 1/5000 volume of the diluted solution to the diluted solution added to each of the prescribed concentrations. Prepared. In addition, a molecular weight of 20,000 was used as the tested compound. 5-8 leaf cabbage leaves were planted in pots, and this was soaked in the test solution for 60 seconds.
  • Composition of the present invention 100 (this tongue). 59. 4 80. 0 Yes
  • the present invention can provide a pest control composition or the like that can obtain a synergistic effect that is more than the additive effect that is expected to be obtained simply by mixing, and that can reduce the amount of drug used.

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne une composition destinée à contrôler un organisme nocif, comprenant une protéine insecticide que Bacillus thuringiensis peut produire et un polyéthylène glycol ayant un poids moléculaire de 2 000 à 800 000 ; et similaire.
PCT/JP2006/303929 2005-02-24 2006-02-23 Composition pour le controle d'organismes nocifs Ceased WO2006090902A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2005-048414 2005-02-24
JP2005048414 2005-02-24

Publications (1)

Publication Number Publication Date
WO2006090902A1 true WO2006090902A1 (fr) 2006-08-31

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PCT/JP2006/303929 Ceased WO2006090902A1 (fr) 2005-02-24 2006-02-23 Composition pour le controle d'organismes nocifs

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WO (1) WO2006090902A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021158420A1 (fr) * 2020-02-05 2021-08-12 Dow Global Technologies Llc Formulations pesticides de bacillus thuringiensis
CN115413678A (zh) * 2022-09-20 2022-12-02 中捷四方生物科技股份有限公司 一种含大丽轮枝孢激活蛋白的可湿性粉剂和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07285804A (ja) * 1994-02-28 1995-10-31 Sumitomo Chem Co Ltd 殺虫剤組成物
US5690951A (en) * 1991-10-31 1997-11-25 Micro Flo Company Bait with hot melt binder
JP2001064103A (ja) * 1999-08-26 2001-03-13 Sankei Kagaku Kk 農園芸用殺虫剤組成物
CN1359622A (zh) * 2002-01-21 2002-07-24 河北省食品企业发展公司 一种生物源复合农药

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5690951A (en) * 1991-10-31 1997-11-25 Micro Flo Company Bait with hot melt binder
JPH07285804A (ja) * 1994-02-28 1995-10-31 Sumitomo Chem Co Ltd 殺虫剤組成物
JP2001064103A (ja) * 1999-08-26 2001-03-13 Sankei Kagaku Kk 農園芸用殺虫剤組成物
CN1359622A (zh) * 2002-01-21 2002-07-24 河北省食品企业发展公司 一种生物源复合农药

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 140, Columbus, Ohio, US; abstract no. 194907, XP003002855 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021158420A1 (fr) * 2020-02-05 2021-08-12 Dow Global Technologies Llc Formulations pesticides de bacillus thuringiensis
CN115209739A (zh) * 2020-02-05 2022-10-18 陶氏环球技术有限责任公司 苏云金芽孢杆菌杀虫剂调配物
CN115209739B (zh) * 2020-02-05 2025-08-05 陶氏环球技术有限责任公司 苏云金芽孢杆菌杀虫剂调配物
CN115413678A (zh) * 2022-09-20 2022-12-02 中捷四方生物科技股份有限公司 一种含大丽轮枝孢激活蛋白的可湿性粉剂和应用

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