WO2006100253A3 - Verfahren zur oligosaccharidsynthese oder zur glykosylierung unter verwendung von glycosyltransferasen und saccharose-analoga - Google Patents

Verfahren zur oligosaccharidsynthese oder zur glykosylierung unter verwendung von glycosyltransferasen und saccharose-analoga Download PDF

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Publication number
WO2006100253A3
WO2006100253A3 PCT/EP2006/060932 EP2006060932W WO2006100253A3 WO 2006100253 A3 WO2006100253 A3 WO 2006100253A3 EP 2006060932 W EP2006060932 W EP 2006060932W WO 2006100253 A3 WO2006100253 A3 WO 2006100253A3
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WO
WIPO (PCT)
Prior art keywords
glycosylation
glycosyl transferases
synthesizing oligosaccharides
saccharide
sucrose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2006/060932
Other languages
English (en)
French (fr)
Other versions
WO2006100253A2 (de
Inventor
Klaus Buchholz
Juergen Seibel
Hans-Joachim Joerdening
Raphael Beine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Technische Universitaet Braunschweig
Original Assignee
Technische Universitaet Braunschweig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technische Universitaet Braunschweig filed Critical Technische Universitaet Braunschweig
Priority to EP06725216A priority Critical patent/EP1861503A2/de
Priority to US11/886,938 priority patent/US20090076258A1/en
Publication of WO2006100253A2 publication Critical patent/WO2006100253A2/de
Publication of WO2006100253A3 publication Critical patent/WO2006100253A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Polymers & Plastics (AREA)
  • Materials Engineering (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Die vorliegende Erfindung betrifft ein enzymatisches Verfahren zur Synthese von Oligosacchariden, bei dem jeweils eine Saccharidgruppe eines Saccharose- Analogons auf ein Akzeptormolekül übertragen wird, beispielsweise zur Glykosylierung einer Hydroxylverbindung, eines Saccharids, Peptids, oder eines Arzneimittels. Das erfindungsgemäße Verfahren nutzt in einem ersten Schritt die enzymatische Synthese eines ß-D-Fructofuranosyl-α-D-Aldopyranosids, in einem zweiten Schritt die enzymatische Übertragung eines der Saccharidreste auf das Akzeptormolekül.
PCT/EP2006/060932 2005-03-22 2006-03-22 Verfahren zur oligosaccharidsynthese oder zur glykosylierung unter verwendung von glycosyltransferasen und saccharose-analoga Ceased WO2006100253A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP06725216A EP1861503A2 (de) 2005-03-22 2006-03-22 Verfahren zur oligosaccharidsynthese oder zur glykosylierung unter verwendung von glycosyltransferasen und saccharose-analoga
US11/886,938 US20090076258A1 (en) 2005-03-22 2006-03-22 Method for synthesizing oligosaccharides and glycosylation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05102284 2005-03-22
EP05102284.6 2005-03-22

Publications (2)

Publication Number Publication Date
WO2006100253A2 WO2006100253A2 (de) 2006-09-28
WO2006100253A3 true WO2006100253A3 (de) 2007-04-26

Family

ID=37024194

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/060932 Ceased WO2006100253A2 (de) 2005-03-22 2006-03-22 Verfahren zur oligosaccharidsynthese oder zur glykosylierung unter verwendung von glycosyltransferasen und saccharose-analoga

Country Status (3)

Country Link
US (1) US20090076258A1 (de)
EP (1) EP1861503A2 (de)
WO (1) WO2006100253A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108384821B (zh) * 2017-12-18 2021-09-14 江苏省农业科学院 一种促进肠道益生菌增殖的低聚糖的制备方法
CA3118909A1 (en) * 2018-11-08 2020-05-14 Kaleido Biosciences, Inc. Oligosaccharide compositions and methods of use thereof
CN113651860A (zh) * 2021-08-25 2021-11-16 合肥工业大学 一种适合肠道益生菌增殖的乳果三糖及其酶法制备方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001090319A2 (en) * 2000-05-25 2001-11-29 Nederlandse Organisatie Voor Toegepast-Wetenschappelijk Onderzoek Fructosyltransferases (inulosucrase and levansucrase) from lactobacillus reuteri

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001090319A2 (en) * 2000-05-25 2001-11-29 Nederlandse Organisatie Voor Toegepast-Wetenschappelijk Onderzoek Fructosyltransferases (inulosucrase and levansucrase) from lactobacillus reuteri

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BÖKER, M. ET AL.: "Kinetics of leucrose formation from sucrose by dextransucrase", BIOTECHNOLOGY AND BIOENGINEERING, vol. 43, no. 9, 1994, pages 856 - 864, XP002415398 *
CHEETHAM, P.S.J. ET AL.: "Synthesis of novel disaccharides by a newly isolated fructosyl transferase from Bacillus subtilis", ENZYME AND MICROBIAL TECHNOLOGY, vol. 11, no. 4, 1989, pages 212 - 219, XP002415396 *
DEMUTH, K. ET AL.: "Oligosaccharide synthesis by dextransucrase: new unconventional acceptors", CARBOHYDRATE RESEARCH, vol. 337, no. 20, 2002, pages 1811 - 1820, XP004392177 *
FEINGOLD, D.S. ET AL.: "Enzymatic synthesis and reactions of a sucrose isomer alpha-D-galactopyranosyl-beta-D-fructofuranoside", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 224, no. 1, 1957, pages 295 - 307, XP002415395 *
MAZZA, J.C. ET AL.: "Synthesis of alpha-D-glycopyranosyl alpha-L-sorbofuranoside, and its use as a D-glucosyl donor", CARBOHYDRATE RESEARCH, vol. 40, no. 2, 1975, pages 402 - 406, XP002415397 *
TANAKA, T. ET AL.: "Structures of Heterooligosaccharides Synthesized by Levansucrase", JOURNAL OF BIOCHEMISTRY, vol. 90, no. 2, 1981, pages 521 - 526, XP008073464 *

Also Published As

Publication number Publication date
EP1861503A2 (de) 2007-12-05
US20090076258A1 (en) 2009-03-19
WO2006100253A2 (de) 2006-09-28

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