WO2006115093A1 - Protein carrier, protein-carrying filter and method for producing the same - Google Patents

Abstract

It is intended to provide a carrier for a protein such as an enzyme, an antibody and the like, which can adsorb and efficiently inactivate various bacteria, fungi, viruses, allergic substances and the like. In order for an enzyme or an antibody substance to effectively function on a filter, a surface treatment agent, in which a composition ratio (alkylene glycol component/hydrocarbon component) is in the range of (50/50) to (100/0) in terms of mass ratio, is used and a polyester substrate containing polyester and an amorphous copolymerized polyester component as constituents of a single sheet is used as a substrate. The protein carrier of the invention can be realized by a simple method of immersing the substrate in a protein solution.

Description

 Specification

 Protein carrier, protein-carrying filter, and production method thereof

 Technical field

 [0001] The present invention relates to a carrier and a filter that carry proteins such as enzymes and antibodies that can adsorb and inactivate various bacteria, molds, viruses, allergen substances, and the like. In more detail, a nanosensor with a protein supported on a carrier, characterized by specifically stabilizing the expression of the protein such as the enzyme, antibody, etc. supported by the fiber surface treatment agent and the fiber component, harmful The present invention relates to a support that can be used for building materials such as wallpaper and curtains that decompose compounds, clothing that decomposes odor components and allergens, and filters that can be suitably used as filters for air purifiers and filter media for masks.

 Background art

 [0002] Various filter media for removing harmful substances such as various bacteria, molds, viruses, and allergen substances have been proposed so far. However, proteins such as enzymes and antibodies are supported on the filter by a simple method, and the protein surface activity and the component components of the base material supply the water necessary for the expression of the protein activity, Proposals of protein carriers and filters that specifically stabilize expression are unprecedented.

 [0003] Patent Document 1 discloses a virus removal filter in which at least one of sialic acid, sialic acid derivatives, saccharides, glycoproteins, and glycolipids containing these is used as a virus trap. Further, Patent Document 2 describes a fiber material having a high degree of microfibrosis, in which at least one fiber captures the virus, a natural receptor for the virus, or a part thereof or a similar one. Disclosed are derivatized with cyanogen bromide to attach the body. However, the method of removing harmful substances in the air by filtration using a filter or physical adsorption using an adsorbent is non-specific and has low accuracy. In addition, in order to prevent the removed harmful substances from re-floating or the growth of harmful substances on the filter to become a new source of contamination, it is necessary to combine the techniques of sterilization and inertness of harmful substances. I must.

[0004] Patent Document 3 is composed of a string that holds a non-woven fabric to which the tea extract component is attached to the ear. As an antiviral mask, Patent Document 4 discloses a filter carrying a water-insoluble polymer anti-allergen agent having a phenolic hydroxyl group and a hygroscopic material. Tea-extracted components and water-insoluble polymers with phenolic hydroxyl groups are known to have antibacterial activity, but they can inactivate specific fungal viruses such as enzymes and antibodies. It has no specific reactivity. A protein such as an enzyme 'antibody can be prepared according to a target harmful substance, and a more reliable effect can be obtained.

 In order to sterilize and inactivate harmful substances on a filter, it is effective to carry a protein such as an enzyme antibody having an ability to sterilize and inactivate harmful substances on the filter. However, a filter in which various enzymes are supported on a substrate by a method such as a covalent bond, an ionic bond, a cross-linking method, and a comprehensive method disclosed in Patent Document 5, Patent Document 6, Patent Document 7, and Patent Document 8, There is a problem that the loading method is complicated and expensive, and it is desired to load a protein by a simple method and to express its activity.

 [0006] Furthermore, Patent Document 4 discloses a method of absorbing moisture necessary for the expression of the activity of the hygroscopic material strength anti-allergen agent attached to the filter surface from the ambient atmosphere, but moisture obtained from the ambient atmosphere is also disclosed. There was a problem that the amount was not necessarily sufficient for protein activity expression.

 [0007] Proteins should have superior characteristics compared to the anti-allergen agents described in Patent Document 3 and Patent Document 4, that is, tea extraction components and water-insoluble polymers having phenolic hydroxyl groups. On the other hand, the activity of protein may be inhibited depending on the hygroscopic material that coexists. Proposals related to substrates and surface treatments optimized for protein activity have been strong.

 Patent Document 1: JP-A-9-234317

 Patent Document 2: Japanese Patent Laid-Open No. 2001-527166

 Patent Document 3: JP-A-8-333271

 Patent Document 4: Japanese Patent Laid-Open No. 2004-290922

 Patent Document 5: WO98 / 04334

 Patent Document 6: Japanese Patent Laid-Open No. 60-49795

Patent Document 7: JP-A-2-41166 Patent Document 8: Japanese Unexamined Patent Publication No. 2003-210919

 Disclosure of the invention

 Problems to be solved by the invention

[0008] The present invention carries a protein such as an enzyme or an antibody capable of adsorbing various bacteria, molds, viruses, allergen substances, and the like and efficiently inactivating them with a carrier and a filter. It is an object to provide a support and a filter.

 Means for solving the problem

[0009] As a result of intensive studies to improve the performance of protein carriers and protein-carrying filters, the present inventors have found that enzymes such as enzymes and antibodies can coexist with specific substrates and surface treatment agents. The inventors have found that the activity expression of proteins such as antibodies is specifically stabilized, and finally completed the present invention. That is, the present invention relates to (1) a protein carrier comprising a carrier and a carrier containing a hydrophilic material, and (2) the hydrophilic material is contained in the molecule. (1) The protein carrier according to (1), which has a polyalkylene glycol component, (3) The amount of the hydrophilic material supported is 0.01% by mass or more based on the base material. The protein carrier according to (1) or (2), (4) the composition of the hydrophilic material having a polyalkylene glycol component in the molecule is represented by a mass ratio of (alkylene glycol component Z hydrocarbon component). (50Z50) to (100ZO), the protein carrier according to (2) or (3), (5) wherein the base material contains polyester fibers and is V (1 ) To (4) V, the protein carrier according to any one of the above, (6) the polyester fiber is an amorphous copolymer polyester (5) The protein carrier according to (5), characterized in that the base material contains silica gel (1) No sol (6) V? (8) The protein carrier according to any one of (1) to (7), characterized in that the substrate contains activated carbon! / (9) (1) Or (8) a protein-carrying filter characterized by having a protein-carrying strength as described in any of the above, (10) (1) No sol (3) V, including the hydrophilic material described in any one of (1), (5) to (8) A method for producing a protein carrier, wherein a protein is supported by impregnating a protein solution with a base material described in V. The invention's effect

 [0010] The protein carrier and filter of the present invention adsorb various bacteria, molds, viruses, allergen substances, etc. by specifically stabilizing the activity expression of proteins such as enzymes and antibody substances. It is possible to inactivate efficiently on the carrier and the filter.

 BEST MODE FOR CARRYING OUT THE INVENTION

 [0011] The filter according to the present invention preferably carries a protein. Proteins can be selectively removed by optimizing their types, so the target bacteria, etc. can be removed with high accuracy with little influence from the presence of other dust etc. outside the removal object. It ’s Kasura and others.

 [0012] In addition, a water-insoluble polymer having a tea extraction component or a phenolic hydroxyl group is poor in workability, such as being not mixed well when it is attached to a carrier having a low affinity for water, resulting in spots. Even if it is difficult to uniformly disperse it on the carrier, and it has excellent inactivation effects such as bacteria by itself, it has the power to reduce its performance as a carrier. Although there are differences depending on the type, it is generally well dissolved in water, has excellent additivity when attached to a substrate, and can be uniformly dispersed throughout the carrier, thus removing harmful substances as a carrier. Improves performance. ・ Stable and stable effect.

 [0013] Proteins such as enzymes and antibodies applicable to the protein carrier of the present invention include lytic enzymes (lysozyme), proteolytic enzymes (proteases), enzymes that oxidize and reduce various allergens (oxide reductase), bacteria Alternatively, antibodies that inactivate fungi, viruses, environmental allergens, and the like can be mentioned, but there is no particular limitation.

[0014] Further, the enzyme here is "a protein biocatalyst produced in living cells", and an antibody is "specifically an antigen produced in vivo by stimulation of an antigen in an immune reaction. `` A generic term for proteins that bind, '' and `` proteins are a group of high-molecular nitrogen-containing organic compounds that are mainly contained in the cells of animals such as animal 'plants' microorganisms '' (Iwanami Biochemistry) Dictionary _3rd edition Iwanami Shoten).

 [0015] It is preferable that the carrier used in the present invention carries a hydrophilic material. This is because protein activity can be promoted by supporting the hydrophilic material.

[0016] Here, the hydrophilic material used in the present invention retains moisture and is necessary for the expression of protein activity. That can provide a sufficient amount of moisture and reaction field, and can exemplify polyacrylic acid polymers, polyvinyl alcohol polymers, hyaluronic acid, etc. Especially if it is a hydrophilic material There is no limitation. A preferable surface treatment agent having a polyalkylene glycol component in the molecule is preferable in terms of performance, cost, and cacheability. That is, a hydrophilic material having a polyalkylene glycol component in the molecule has hydrophilicity and enhances the activity of protein, and at the same time has excellent affinity with a polymer material such as polyester, so that A film can be formed uniformly, and the expression of activity can be promoted throughout the protein carried on the substrate.

 [0017] The surface treatment agent having a polyalkylene glycol component in the molecule includes polyalkylene glycol, polyalkylene glycol 'alkyl ether, polyalkylene glycol · alkylphenol ether, polyalkylene glycol' alkyl ester, polyalkylene glycol. Alkyl ether. Sulfate ester salts of polyalkylene glycols, phosphoric acid ester salts of alkyl ethers, polyalkylene glycol 'alkyl tertiary amines, polyether-polyester block copolymers containing a polyalkylene glycol component, etc. Alkylene glycols and polyalkylene glycols' alkyl ethers are preferable because they have good cache properties when uniformly applied to the substrate surface. In order to stably express the activity of a protein such as an enzyme / antibody supported on the substrate surface, it is important that the surface treatment agent is uniformly applied to the substrate surface.

 Here, the alkylene glycol component refers to ethylene glycol, propylene glycol, butylene glycol and the like, and ethylene glycol is particularly preferable for use for this purpose because of its high hydrophilicity. In addition, the average molecular weight of the polyalkylene glycol is preferably in the range of 100 force to 5000 and good workability.

[0019] Polyalkylene glycol alkyl ether, polyalkylene glycol alkyl phenyl ether, polyalkylene glycol 'alkyl ester, polyalkylene glycol alkyl ether sulfate, polyalkylene glycol alkyl ether phosphate Alkyl groups contained in salts, polyalkylene glycols, alkyl tertiary amines and the like have a carbon number in the range of 10 to 18, and are particularly preferred because they are easily available (12 lauryl) and 18 (stearyl). Also, the amount of this component is the amount of hydrocarbon component. It is defined as

[0020] A polyether 'polyester block copolymer containing a polyalkylene glycol component is a terephthalic acid and Z or isophthalic acid, a lower alkylene glycol, a polyalkylene glycol, and a polyether that also has its monoether power. More specifically, terephthalic acid 'alkylene glycol' polyalkylene glycol, terephthalic acid. Isophthalic acid. Alkylene glycol. · Alkylene glycol · Polyalkylene glycol monoether. Here, the molar ratio of terephthalic acid to isophthalic acid is preferably in the range of (terephthalic acid Z isophthalic acid) = (40Z60) to (100ZO). The average molecular weight of the block copolymer is the force usually 2000 forces et 20000 depending on the molecular weight of the alkylene glycol component comprising, 1500 Mashi Bogo囲力^s girls forces et al 8000 <50 wt _^0/0 or more force S Preferably occupied by a polyalkylene glycol component. When the polyalkylene glycol component is less than 50% by weight, the ability to provide water and a reaction field necessary for the expression of protein activity is lowered, which is not preferable.

 [0021] In addition to the above-described surface treating agent having a polyalkylene glycol component in the molecule, other treating agents can be used in combination with the base material of the present invention. However, the hydrocarbon component of the oil agent is hydrophobic and adsorbs to the hydrophobic group of the protein to inhibit its activity. Therefore, the sum of the alkyldaricol component contained in the molecules of all the surface treatment agents used. Ratio force of the sum of the hydrocarbon component and the mass ratio (alkylene glycol component Z hydrocarbon component) = (50/5 0) to (100Z0) is preferred! / ,.

 [0022] However, from the viewpoint of fiber wetting performance and antistatic performance, the preferred range of the mass ratio is (60-40) to (90Z10).

[0023] The amount of the hydrophilic material supported is preferably 0.01% by weight or more based on the base material in order to obtain a sufficient effect. On the other hand, if the amount exceeds 50% by weight, the effect of improving the stability of protein activity can not be expected even if the amount of adhesion is increased further. When it does, hardness spots etc. generate | occur | produce and it is not preferable. More preferably, it is 0.05 to 30% by weight, and still more preferably 0.1 to 10% by weight. [0024] The hydrophilic material may be applied to the carrier either before or after carrying the protein such as an enzyme or an antibody. Specifically, when manufacturing a sheet constituting the substrate, a method of applying to a fiber using a processing roller or the like, or a predetermined surface treatment agent is mixed with a protein solution, and the protein and the surface treatment agent are simultaneously supported. And the like.

 [0025] The material of the base material of the protein carrier of the present invention preferably contains a polyester fiber. More preferably, the polyester fiber contains an amorphous copolyester component. Polyester containing an amorphous copolymerized polyester component can uniformly support hydrophilic materials with good water wettability over the entire fiber surface, and processability when supporting proteins such as enzymes and antibodies. Because it is good. Furthermore, the polyester containing the amorphous copolymerized polyester component has an appropriate humidity control property. For this reason, the surface treatment agent having a hydrophilic material from the polyester substrate containing the amorphous copolymerized polyester component and the surrounding atmosphere. By absorbing and retaining moisture, it provides a reaction field for the protein and stabilizes the expression of the activity, thereby providing a protein-carrying filter that highly stabilizes the expression of the activity of the protein. The moderate humidity control mentioned here is a performance capable of providing water necessary for the expression of the activity of the protein supported on the substrate surface. If the amount of water provided to the protein is too large, the protein will be prematurely deteriorated, and if it is too small, the activity will not be expressed. In addition, the amount of water provided to the protein present on the surface of the substrate has no direct correlation with the water content representing the amount of water present in the fiber.

 [0026] The amorphous copolymerized polyester component is preferably obtained by adding a copolymer component during the formation of a polyester by a polycondensation reaction of an aromatic dicarboxylic acid and a dalycol component. More preferably, it is based on terephthalic acid and ethylene glycol, and examples of the copolymer component include isophthalic acid, adipic acid, sebacic acid, azelaic acid, dodecanoic acid, diethylene glycolone, propanediole, butanediole, pentanediol. -Noreth, hexanediol, polyethylene glycol, neopentyl dallicol and the like. Such an amorphous copolyester has a glass transition point in the range of Tg = 50 to 100 ° C. and does not show a clear crystal melting point.

[0027] In addition, the present invention supports proteins such as enzymes and antibody substances, and stabilizes the expression of the activity thereof. The amorphous copolymerized polyester component is terephthalic acid, isophthalic acid, ethylene. It is preferable to include glycol and diethylene glycol. Copolymer molar power (terephthalic acid Z isophthalic acid) = (40Z60) force (80Z20), (ethylene glycol Z diethylene glycol) = (70Z30) More preferably.

 [0028] The substrate containing the amorphous copolymerized polyester component is preferably a composite of the above-mentioned amorphous copolymerized polyester component and the polyalkylene terephthalate-based polyester component. Specific examples of the polyalkylene terephthalate polyester include polyethylene terephthalate and polybutylene terephthalate. When the base material is also composed of fiber force, it is possible to adopt a form of a sheath 'core type, an eccentric sheath' core type, a side 'by' side type, etc. that are preferably composite fibers. Among them, a sheath type core composite fiber having amorphous copolymer polyester as a sheath component and polyethylene terephthalate as a core component is preferred in terms of processability. The composite ratio of the amorphous copolyester component is 10 to 90%, and both performance and processability are preferably 30 to 70%.

 [0029] In addition to the above materials, there are no particular limitations on the power of the base material of the protein-carrying filter of the present invention, such as acrylic, nylon, polyolefin, rayon, cellulose, pulp and the like.

 [0030] The fineness of the fiber should be adjusted within the range of 1 to 30 dtex.

[0031] The form of the substrate is a nonwoven fabric such as a film, a spunbond nonwoven fabric, a spunlace nonwoven fabric, a needle punch nonwoven fabric, a melt blown nonwoven fabric, a flash spun nonwoven fabric, a thermal bond nonwoven fabric, a chemical bond nonwoven fabric, a stitch bond nonwoven fabric, and a wet papermaking nonwoven fabric. Or the thing of the form of sheets, such as textiles and paper, is mentioned. Further, when the substrate is in the form of a sheet, the basis weight is not particularly limited, but a preferred range is 10 to 20 OgZm ² . If it is less than lOgZm ² , there is a possibility that inactivated objects such as various bacteria, molds, viruses, and allergen substances cannot be captured sufficiently. On the other hand, if it exceeds 200 gZm ^2, the pressure loss when used as a filter increases, which is not preferable.

[0032] As a method for supporting a protein such as an enzyme or an antibody substance on a substrate, a method in which the substrate is impregnated with an aqueous protein solution of an appropriate concentration and dried at an appropriate temperature and time is a preferred example. The aqueous protein solution used here has a pH suitable for the expression of the activity of each protein. Preferably it is adjusted. This is because a protein has a pH suitable for activity expression depending on the type of protein, and usually it is not around that pH, and sufficient activity expression cannot be obtained. It is important that the drying temperature and time are such that the protein to be carried is not inactivated by denaturation. Proteins vary greatly in heat resistance depending on the type. Usually, drying at a high temperature results in better processing efficiency in a shorter time, but it is preferable to determine the drying conditions in consideration of the heat resistance of the protein.

[0033] In general, the activity of proteins such as enzymes and antibodies is not expressed in a dry state. Therefore, when a protein such as an enzyme or an antibody is supported on a substrate and used, it is necessary to supply water. The inventors of the present invention have the role that the hydrophilic material contained in the surface treatment agent of the fibers constituting the base sheet retains moisture in the surrounding atmosphere and provides a protein reaction field.

It was found that the expression of activity is stabilized. In addition, the base material of the present invention is imparted with an appropriate humidity control action by adding a specific copolymer component to the inherently hydrophobic polyester.

This is stable because of the repeated expression of protein activity. The polyester base material is also a material that can supply water to the hydrophilic material and supply a sufficient amount of water to the protein for activity expression.

 [0034] The carrier of the present invention is preferably a filter for the following reasons. In other words, with conventional filters that use physical adsorption, it was difficult to remove and decompose low-boiling components. However, if a filter carrying a protein that decomposes the target substance is used, even low-boiling components can be removed. 'Disassembly is possible. Furthermore, with conventional filters, trapped bacteria etc. grow in the filter using the trapped dusts at the same time, generating odor or reaching the downstream part of the filter and causing secondary contamination. However, this problem can be solved by using a filter carrying a protein that has the effect of killing bacteria.

 Example

 Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited thereto.

[0036] (Manufacture of substrate 1)

60% terephthalic acid in molar ratio of acid component as an amorphous copolyester of sheath component %, Isophthalic acid 0%, diol component in a molar ratio of ethylene glycol 95%, ethylene glycol 5% copolymerized, intrinsic viscosity 0.56, Tg 64 ° C amorphous Copolyester, polyethylene terephthalate with an intrinsic viscosity of 0.64, Tg of 67 ° C, Tm of 256 ° C as the core component, each pellet was dried under reduced pressure, and then sheathed-core type composite spinning machine Combined spinning, drawing and cutting with a drawing machine for short fibers, and a sheath ratio of about 2.2 dt ex with a composite ratio of mass ratio (sheath Z core) = (50Z50) according to a conventional method. A mold composite fiber was obtained. For the hydrophilic material, polyoxyethylene alkyl ether and C12 alkyl phosphate potassium salt are used. The hydrophilic material as a whole has a mass ratio (alkylene glycol component Z hydrocarbon component) = (70Z30). Was applied when winding after stretching. Here, the hydrophilic material was used in such an amount that 0.1% by mass finally remained with respect to the base material. Next, this fiber was cut into 50 mm, a web was prepared by carding, and then a needle punch process was performed to obtain a substrate having a basis weight of 30 g / m ² .

 [0037] (Manufacture of base material 2)

Polyethylene terephthalate pellets with an intrinsic viscosity of 0.64, Tg of 67 ° C, and Tm of 256 ° C are dried under reduced pressure, then spun by a single fiber spinning machine, drawn by a single fiber drawing machine, and single yarn A polyethylene terephthalate fiber having a fineness of about 2.2 dtex was obtained. For the hydrophilic material, polyoxyethylene alkyl ether and C12 alkyl phosphate potassium salt are used, and the hydrophilic material as a whole has a mass ratio (alkylene glycol component Z hydrocarbon component) = (70Z30) after drawing the fiber. Granted when winding. Here, the hydrophilic component was used in such an amount that 0.1% by mass finally remained with respect to the substrate. Next, this fiber was cut into 50 mm, a web was formed by carding, and then needle punching was performed to obtain a substrate having a basis weight of 30 g / m ² .

 [0038] (Manufacture of base material 3)

Polyethylene terephthalate pellets with an intrinsic viscosity of 0.64, Tg of 67 ° C, and Tm of 256 ° C are dried under reduced pressure, then spun by a single fiber spinning machine, drawn by a single fiber drawing machine, and single yarn A polyethylene terephthalate fiber having a fineness of about 2.2 dtex was obtained. The hydrophilic material is C12 alkyl phosphate potassium salt. When the entire hydrophilic material has a mass ratio (alkylene glycol component Z hydrocarbon component) = (0Z100), the fiber is wound after drawing. Granted. Here, the hydrophilic component was used in such an amount that 0.1% by mass finally remained with respect to the carrier sheet. Next, this fiber was cut into 50 mm, a web was formed by carding, and then needle punching was performed to obtain a carrier sheet having a basis weight of 30 g / m ² .

 [0039] (Enzyme activity test: Test example 1)

 Sprinkle granules dried with freeze-dried Micrococcus luteus on the protein-supporting finoleters of Examples and Comparative Examples, and after inverting, remove the excess granules, and then let stand for 24 hours in a desiccator maintained at 90% humidity . Thereafter, it was observed with a low vacuum scanning electron microscope, and the degree of lysis of Luteus was visually observed. When the sphere shape of the bacterium collapsed, it was determined that the lysis activity was present, and when the sphere shape remained, it was determined that there was no lysis activity.

 [0040] (Influenza virus inactivity test: Test example 2)

 The protein-carrying filters of Examples and Comparative Examples cut into 33 mm squares were used as test pieces, placed in sealed containers, and sprayed with an aqueous solution containing 0.5 mg of influenza virus per ml in 0.5 ml containers. The treatment was performed at room temperature for 15 hours. Thereafter, an aqueous solution containing 1 mg / ml of inactivated influenza virus is sprayed into a 0.5 ml container to block the antibody, and the treated test piece is washed out with 9 ml of phosphate buffer solution. Was inoculated into the egg for 10 days, cultured at 37 ° C for 48 hours, CAM solution was collected, HA test was performed, and virus infection titer EID (50% egg-infectiv) was determined by Karber method.

 50

 e doses) was calculated.

[Example 1]

 lOOmM Tris— Substrate 1 was soaked in an aqueous solution of 1% by weight of lysing enzyme lysozyme in HC1 buffer (pH 8.0) for 1 hour, then removed to remove excess adhering moisture. The material was dried for 10 minutes in a dryer maintained at a temperature of 60 ° C. to prepare a protein-carrying filter.

 [Example 2]

 Using the substrate 2, a protein-carrying filter was prepared in the same process as in Example 1.

 [0043] (Comparative Example 1)

 A protein-carrying filter was prepared using the substrate 3 in the same process as in Example 1.

[0044] (Example 3) Using lOOmM phosphate buffer (pH 7.0), prepare an aqueous solution of influenza virus antibody concentration 5mgZ lml, soak substrate 1 in the aqueous solution for 1 hour, and then remove it to remove excess adhering moisture. The substrate was dried for 10 minutes in a drier maintained at a temperature of 60 ° C. to prepare a protein-carrying filter.

 [0045] (Example 4)

 A protein-carrying filter was prepared using the substrate 2 in the same process as in Example 3.

 [Comparative Example 2]

 A protein-carrying filter was prepared using the substrate 3 in the same process as in Example 3.

 Examples [0047] For the protein-carrying filters of Example 2 and Comparative Example 1, the enzyme activity test shown in Test Example 1 was performed. As a result, in Example 2 and Example 2, it was confirmed that Rutheus was disintegrated due to the lysing action of lysozyme. In Example 1 in particular, the effect was high. In Comparative Example 1, the decay of Luteus was not confirmed. Further, the influenza virus inactivation test shown in Test Example 2 was carried out on the protein-carrying filters of Example 3, Example 4, and Comparative Example 2. As a result, the viral infectivity values of Example 3 and Example 4 were EID = 0.

 50

10 ^{4, 5,} viral infectivity titer of Comparative Example 2 is ^{EID = 0. 5 X 10 8} · 5, Example 3 and the actual

 50

 It was confirmed that the amount of activated virus by the protein-carrying filter of Example 4 was reduced by 4 orders of magnitude, that is, 99.99% inactivation. Table 1 shows the outline of the test example 1 and Table 2 shows the outline of the practical examples and comparative examples and the comprehensive evaluation results for the test example 2.

[0048] Test Example 1

 [table 1]

[0049] Test Example 2

[Table 2] Substrate Surface treatment simultaneous IJ

 Protein activity Crystal

 (Alkylene Cricole component: Charcoal hydrogen component)

 Polymerization component

 Including actual salmon cans 3 (70:30) 〇

 Example 4 Not included ヽ (70:30) 〇

 Comparative example 2 not included (Ο: 1 ΟΟ) Industrial applicability

 The protein-carrying body of the present invention realizes high performance because the specific composition of the base material and the surface treatment agent contributes to the expression of the activity of the protein such as the carried enzyme or antibody. Moreover, since protein can be loaded by a simple method, it can be easily industrially used.

Claims

The scope of the claims  [1] A protein carrier comprising a substrate and a carrier containing a protein and a hydrophilic material.  2. The protein carrier according to claim 1, wherein the hydrophilic material has a polyalkylene glycol component in the molecule. [3] The protein carrier according to claim 1 or 2, wherein the amount of the hydrophilic material supported is 0.01% by mass or more based on the base material. [4] The composition ratio of the hydrophilic material having a polyalkylene glycol component in the molecule (alkylene glycol component Z hydrocarbon component) is (50Z50) to (100ZO) in mass ratio. Or the protein carrier according to 3. [5] The protein carrier according to claims 1 to 4, wherein the base material contains polyester fiber. 6. The protein carrier according to claim 5, wherein the polyester fiber strength is a fiber containing an amorphous copolyester component. [7] The protein carrier according to any one of [1] to [6], wherein the base material contains silica gel.  [8] The protein carrier according to any one of [1] to [7], wherein the substrate contains activated carbon.  [9] A protein-carrying filter comprising the protein-carrying member according to any one of claims 1 to 8.  [10] The method according to claim 1, comprising the hydrophilic material according to any one of claims 1 to 3, and a protein solution supported by impregnating the substrate according to any one of claims 5 to 8 with a protein solution. A method for producing a protein carrier.