WO2006119066A3 - Amplification and cloning of single dna molecules using rolling circle amplification - Google Patents

Amplification and cloning of single dna molecules using rolling circle amplification Download PDF

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Publication number
WO2006119066A3
WO2006119066A3 PCT/US2006/016349 US2006016349W WO2006119066A3 WO 2006119066 A3 WO2006119066 A3 WO 2006119066A3 US 2006016349 W US2006016349 W US 2006016349W WO 2006119066 A3 WO2006119066 A3 WO 2006119066A3
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WO
WIPO (PCT)
Prior art keywords
amplification
rolling circle
cloning
dna molecules
single dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/016349
Other languages
French (fr)
Other versions
WO2006119066A2 (en
Inventor
Clyde A Hutchison Iii
Hamilton O Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
J Craig Venter Institute Inc
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J Craig Venter Institute Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE602006015633T priority Critical patent/DE602006015633D1/en
Priority to AT06751837T priority patent/ATE474937T1/en
Priority to HK08107959.6A priority patent/HK1112490B/en
Priority to BRPI0607661-0A priority patent/BRPI0607661A2/en
Priority to US11/919,515 priority patent/US8497069B2/en
Priority to CN200680024024.4A priority patent/CN101213311B/en
Priority to EP06751837A priority patent/EP1885880B1/en
Priority to DK06751837.3T priority patent/DK1885880T3/en
Application filed by J Craig Venter Institute Inc filed Critical J Craig Venter Institute Inc
Priority to AU2006242387A priority patent/AU2006242387B2/en
Priority to CA2607532A priority patent/CA2607532C/en
Publication of WO2006119066A2 publication Critical patent/WO2006119066A2/en
Publication of WO2006119066A3 publication Critical patent/WO2006119066A3/en
Priority to IL187002A priority patent/IL187002A/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates, e.g., to a method for amplifying a small number of copies (e.g. a single copy) of a single-stranded circular DNA molecule (e.g. having a size of about 5-6 kb) by an isothermal rolling circle mechanism, using random or partially random primers and a F29-type DNA polymerase. The method, which can also be used for amplifying DNAs by non-rolling types of multiple displacement amplification, comprises incubating the reaction components in a small volume, e.g. about 10µl or less, such as about 0.6µl or less. The degree of amplification can be about 109 fold, or higher. A method for cell-free cloning of DNA, using the rolling circle amplification method of the invention, is described.
PCT/US2006/016349 2005-04-29 2006-05-01 Amplification and cloning of single dna molecules using rolling circle amplification Ceased WO2006119066A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
EP06751837A EP1885880B1 (en) 2005-04-29 2006-05-01 Amplification and cloning of single dna molecules using rolling circle amplification
HK08107959.6A HK1112490B (en) 2005-04-29 2006-05-01 Amplification and cloning of single dna molecules using rolling circle amplification
BRPI0607661-0A BRPI0607661A2 (en) 2005-04-29 2006-05-01 amplification and cloning of single dna molecule using rolling circle amplification
US11/919,515 US8497069B2 (en) 2005-04-29 2006-05-01 Amplification and cloning of single DNA molecules using rolling circle amplification
CN200680024024.4A CN101213311B (en) 2005-04-29 2006-05-01 Amplification and cloning of single DNA molecules using rolling circle amplification
DK06751837.3T DK1885880T3 (en) 2005-04-29 2006-05-01 Amplification and cloning of single DNA molecules using rolling circle amplification
AU2006242387A AU2006242387B2 (en) 2005-04-29 2006-05-01 Amplification and cloning of single DNA molecules using rolling circle amplification
DE602006015633T DE602006015633D1 (en) 2005-04-29 2006-05-01 AMPLIFICATION AND CLONING OF INDIVIDUAL DNA MOLECULES BY ROLLING CIRCLE AMPLIFICATION
AT06751837T ATE474937T1 (en) 2005-04-29 2006-05-01 AMPLIFICATION AND CLONING OF INDIVIDUAL DNA MOLECULES USING ROLLING CIRCLE AMPLIFICATION
CA2607532A CA2607532C (en) 2005-04-29 2006-05-01 Amplification and cloning of single dna molecules using rolling circle amplification
IL187002A IL187002A (en) 2005-04-29 2007-10-29 Amplification and cloning of single dna molecules using rolling circle amplification

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US67585005P 2005-04-29 2005-04-29
US60/675,850 2005-04-29
US72207005P 2005-09-30 2005-09-30
US60/722,070 2005-09-30
US72530005P 2005-10-12 2005-10-12
US60/725,300 2005-10-12

Publications (2)

Publication Number Publication Date
WO2006119066A2 WO2006119066A2 (en) 2006-11-09
WO2006119066A3 true WO2006119066A3 (en) 2007-03-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/016349 Ceased WO2006119066A2 (en) 2005-04-29 2006-05-01 Amplification and cloning of single dna molecules using rolling circle amplification

Country Status (11)

Country Link
US (1) US8497069B2 (en)
EP (1) EP1885880B1 (en)
CN (1) CN101213311B (en)
AT (1) ATE474937T1 (en)
AU (1) AU2006242387B2 (en)
BR (1) BRPI0607661A2 (en)
CA (1) CA2607532C (en)
DE (1) DE602006015633D1 (en)
DK (1) DK1885880T3 (en)
IL (1) IL187002A (en)
WO (1) WO2006119066A2 (en)

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Also Published As

Publication number Publication date
EP1885880A2 (en) 2008-02-13
IL187002A0 (en) 2008-02-09
EP1885880B1 (en) 2010-07-21
CA2607532C (en) 2013-03-12
WO2006119066A2 (en) 2006-11-09
HK1112490A1 (en) 2008-09-05
BRPI0607661A2 (en) 2009-09-22
IL187002A (en) 2012-03-29
US20090176280A1 (en) 2009-07-09
DK1885880T3 (en) 2010-11-08
CN101213311A (en) 2008-07-02
CN101213311B (en) 2013-02-13
CA2607532A1 (en) 2006-11-09
AU2006242387B2 (en) 2011-01-06
US8497069B2 (en) 2013-07-30
ATE474937T1 (en) 2010-08-15
DE602006015633D1 (en) 2010-09-02
AU2006242387A1 (en) 2006-11-09

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