WO2007102713A1 - Système de détection pour biopuce combo-optique comprenant un actionneur de moteur à bobine acoustique - Google Patents

Système de détection pour biopuce combo-optique comprenant un actionneur de moteur à bobine acoustique Download PDF

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Publication number
WO2007102713A1
WO2007102713A1 PCT/KR2007/001143 KR2007001143W WO2007102713A1 WO 2007102713 A1 WO2007102713 A1 WO 2007102713A1 KR 2007001143 W KR2007001143 W KR 2007001143W WO 2007102713 A1 WO2007102713 A1 WO 2007102713A1
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WO
WIPO (PCT)
Prior art keywords
bio
actuator
focusing
chip
area
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2007/001143
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English (en)
Inventor
Soo-Kyung Kim
Kyung-Ho Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanostorage Co Ltd
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Nanostorage Co Ltd
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Filing date
Publication date
Application filed by Nanostorage Co Ltd filed Critical Nanostorage Co Ltd
Publication of WO2007102713A1 publication Critical patent/WO2007102713A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G01N21/6454—Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk

Definitions

  • the present invention relates to a combo-optical bio-chip detection system using a voice-coil motor actuator, and more particularly to a combo-optical bio-chip detection system using a voice-coil motor actuator, which implements a detection actuator and a focusing actuator in the form of a single device, performs an auto-focusing process in real time, and at the same time scans a bio-chip.
  • a voice-coil motor actuator which implements a detection actuator and a focusing actuator in the form of a single device, performs an auto-focusing process in real time, and at the same time scans a bio-chip.
  • a bio-chip is indicative of a biochemical semiconductor in which a biomaterial (e.g., protein, DNA, antibody, enzyme, microbe, animal- and plant- cell and organ, nervous cell, etc.) is integrated on a substrate made of a glass or silicon at high density.
  • a biomaterial e.g., protein, DNA, antibody, enzyme, microbe, animal- and plant- cell and organ, nervous cell, etc.
  • the bio-chip is called a protein chip, a DNA chip, a genetic chip, a cell chip, and a bio- sensor, etc.
  • the bio-chip can be applied to a variety of technical fields, for example, a genetic- function analysis, a clinical diagnosis, an antibiotic-tolerance inspection, medicinal sensitivity inspection, real-child confirmation, and animal and plant quarantines, etc.
  • an analysis method using the above-mentioned bio-chip controls the bio-chip to react to a blood or urine of a specific user to be inspected
  • the bio-chip is configured by integrating not only a protein capable of being reacting to a specific disease or symptoms, but also a fluorescent material.
  • the above-mentioned analysis method analyzes manifestation of the fluorescent material by the reaction between the bio-chip's protein and the protein contained in the blood or urine, such that it can determine the presence or absence of the disease or symptom.
  • the bio-chip for diagnosing a specific disease or symptom is called a diagnosis kit. Due to the promptness and accuracy of the diagnosis kit, the bio-chips are being widely introduced to the market throughout the world.
  • the bio-chip is being analyzed by either an examination with the naked eyes of an analyzing user or a microscope examination.
  • the naked-eye examination requires good dexterity and many experiences of the user because it wholly depends upon the user's personal decision and experiences, such that the reliability of the resultant data may be deteriorated by errors of the user.
  • the accuracy of the analysis method greatly depends upon the user's fatigue and analysis environments, etc.
  • the microscope examination can increase the reliability of data as compared to the naked-eye examination, however, it may unavoidably require a long period of time and much manpower. Disclosure of Invention
  • the present invention has been made in view of the above problems, and it is an object of the present invention to provide a combo-optical bio-chip detection system using a voice-coil motor actuator, which implements a detection actuator and a focusing actuator in the form of a single device, and scans a bio-chip simultaneously while performing a real-time auto-focusing function.
  • a combo-optical bio-chip detection system using a voice-coil motor actuator comprising: a substrate including a bio-chip area and a CD/DVD area; an actuator including: a focusing actuator for outputting a focusing beam to the CD/DVD area of the substrate to generate a focusing error signal, and outputting the focusing error signal, and a detection actuator for performing a focusing function upon receiving the focusing error signal from the focusing actuator such that it establishes synchronization with the focusing actuator in real time; and a controller for converting a bio-chip image signal received from the detection actuator into a bio- analysis signal, and outputting the bio-analysis signal.
  • FIG. 1 is a block diagram illustrating a combo-optical bio-chip detection system using a voice-coil motor actuator according to the present invention
  • FIG. 2 is a structural diagram illustrating a detection actuator and a focusing actuator according to the present invention
  • FIG. 3 is a conceptual diagram illustrating a correlation between the detection actuator and the focusing actuator according to the present invention
  • FIG. 4 is a structural diagram illustrating the detection actuator of FIG. 2 according to the present invention.
  • FIG. 5 is a conceptual diagram illustrating a circular shield lens of FIG. 4 according to the present invention.
  • FIG. 6 is a conceptual diagram illustrating beam areas according to the present invention. Mode for the Invention
  • FIG. 1 is a block diagram illustrating a combo-optical bio-chip detection system using a voice-coil motor actuator according to the present invention.
  • FIG. 2 is a structural diagram illustrating a detection actuator and a focusing actuator according to the present invention.
  • FIG. 3 is a conceptual diagram illustrating a correlation between the detection actuator and the focusing actuator according to the present invention.
  • FIG. 4 is a structural diagram illustrating the detection actuator of FIG. 2 according to the present invention.
  • FIG. 5 is a conceptual diagram illustrating a circular shield lens of FIG. 4 according to the present invention.
  • FIG. 6 is a conceptual diagram illustrating beam areas according to the present invention.
  • the combo-optical bio-chip detection system using the voice- coil motor actuator includes: a substrate 100 including a bio-chip area 110 and a CD/ DVD area 120; an actuator 290 for illuminating a beam to the bio-chip area 110 to generate a bio-chip image signal; a controller 300 for converting the bio-chip image signal received from the actuator 290 into a bio-analysis signal, and outputting the bio- analysis signal to a display 310; and a servo unit 400.
  • the actuator 290 includes a focusing actuator 250 and a detection actuator 200.
  • the focusing actuator 250 outputs a focusing beam to the CD/DVD area 120 of the substrate 100 to generate a focusing error signal, and outputs the focusing error signal.
  • the detection actuator 200 performs a focusing function upon receiving the focusing error signal from the focusing actuator 250 such that it establishes synchronization with the focusing actuator 250 in real time.
  • the value of is indicative of a predetermined value for correcting a tilt generated from the bio-chip and the CD/DVD area in real time during an auto-focusing process.
  • ⁇ is indicative of a tilt angle.
  • the tilt is indicative of an error generated during the processing or assembling of a machine, and is synchronized with an auto- focusing value generated during the auto-focusing of the CD/DVD area, such that it is corrected.
  • the detection actuator 200 includes: a first light source 210 for illuminating a beam of a first wavelength on the bio-chip area 110; a second light source 212 for illuminating a beam of a second wavelength on the bio-chip area 100; a circular shield lens 220 for selecting a light signal having several light-emitting fluorescent wavelengths from among a plurality of optical signals illuminated on the bio-chip area 110, and performing the focusing function; a detection head 228 for detecting the bio- analysis signal from a first area or a second area of the circular shield lens 220; and a voice-coil motor actuator 222 for enabling the first light source 210 to be focused on the first area of the circular shield lens 220 upon receiving the focusing error signal from the focusing actuator 250, or enabling the second light source 212 to be focused on the second area of the circular shield lens 220 upon receiving the focusing error signal from the focusing actuator 250.
  • the circular shield lens 220 has different Numerical Apertures (NAs) according to lens areas.
  • NAs Numerical Apertures
  • the circular shield lens 220 focuses laser beams having different wavelengths owned by Cy3 and Cy5 on different areas, respectively, such that the Cy3 and the Cy5 can acquire their focal points at similar locations.
  • the controller 300 changes a spherical aberration of the circular shield lens 220 according to wavelengths of the first and second light sources 210 and 220, and changes a moving distance of the voice-coil motor actuator 222, such that the detection head 228 can detect the bio-analysis signal.
  • the focusing actuator 250 outputs a laser beam for a CD or DVD to the CD/DVD area 120, receives the laser beam reflected from the CD or DVD using a photo-diode (PD), and performs the focusing process.
  • PD photo-diode
  • the focusing actuator 250 outputs the focusing error signal to the detection actuator
  • the detection actuator 200 after performing the focusing process.
  • the detection actuator 200 performs the focusing function according to the focusing error signal. In other words, the detection actuator 200 is synchronized with the focusing actuator 250 in real time.
  • the controller 300 controls the servo-unit 400 including the bio-chip area 110 upon receiving a bio-chip analysis request from the user, rotates the substrate 100 by controlling the detection actuator 200, and at the same time controls the second light source 212 to generate a second beam.
  • the second beam generated from the second light source 212 is indicative of a laser beam having a wavelength of 554nm, is reflected from a splitter 214, and is then illuminated on the bio-chip 100 via a prism 216, a collimator lens 218, and the circular shield lens 220.
  • the Cy3 sample of the bio-chip area 110 generates a fluorescent signal by the second beam.
  • the second beam is focused on a first area as shown in FIGS. 3 and 4.
  • the fluorescent signal generated from the Cy3 sample is applied to the prism 216 via the circular shield lens 220 and the collimator lens 218, is reflected from the prism 216, and is then applied to the detection head 228 via a focusing lens 224 and a radiation filter 226.
  • the detection head is indicative of a light-emitting fluorescent wavelength head, and is composed of a PMT or APD.
  • the bio-analysis signal detected by the detection head 228 is applied to the controller 300, and is then displayed on a display (not shown), such that the user can recognize a diagnostic result.
  • the first beam generated from the first light source 210 is indicative of a laser beam having a wavelength of 635nm, is reflected from the splitter 214, and is then illuminated on the bio-chip 100 via the prism 216, the collimator lens 218, and the circular shield lens 220.
  • the Cy5 sample of the bio-chip area 100 generates a fluorescent signal by the first beam.
  • the second beam is focused on the first area as shown in FIGS. 3 and 4.
  • the fluorescent signal generated from the Cy5 sample is applied to the prism 216 via the circular shield lens 220 and the collimator lens 218, is reflected from the prism 216, and is then applied to the detection head 228 via the focusing lens 224 and the radiation filter 226.
  • the bio-analysis signal detected by the detection head 228 is applied to the controller 300, and is then displayed on a display (not shown), such that the user can recognize a diagnostic result.
  • the combo-optical bio-chip detection system using the voice-coil motor actuator combines a voice-coil motor actuator combo optical pickup technology and a bio-chip detection technology into a single head device, such that it can implement the DNA chip detection technology using the single optical system.
  • the combo-optical bio-chip detection system according to the present invention can be very precisely driven by a high-speed voice-coil motor optical pickup actuator, such that it can precisely analyze bio-chip data.
  • the combo-optical bio-chip detection system implements a bio-chip precise-recording/reproducing mechanism, is configured in the form of a small-sized product with low production costs.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

La présente invention concerne un système de détection pour biopuce combo-optique qui utilise un actionneur de moteur à bobine acoustique. Un actionneur de détection et un actionneur de mise au point sont configurés sous forme d'un dispositif unique, de sorte que ce dispositif unique effectue une mise au point automatique en temps réel et explore une biopuce. Le système de détection de biopuce combo-optique comprenant un actionneur de moteur à bobine acoustique comprend un substrat comportant une région de biopuce et une région CD/DVD, un actionneur et une unité de commande. L'actionneur comprend: un actionneur de mise au point qui produit un faisceau de mise au point sur la région CD/DVD du substrat pour générer un signal d'erreur de mise au point et pour produire le signal d'erreur de mise au point ainsi qu'un actionneur de détection qui réalise une fonction de mise au point lorsqu'il reçoit de l'actionneur de mise au point le signal d'erreur de mise au point de sorte qu'il établisse la synchronisation avec l'actionneur de mise au point en temps réel. L'unité de commande convertit le signal d'image de la biopuce reçu en provenance de l'actionneur de détection sous forme d'un signal d'analyse de la biopuce et produit le signal d'analyse de la biopuce.
PCT/KR2007/001143 2006-03-08 2007-03-08 Système de détection pour biopuce combo-optique comprenant un actionneur de moteur à bobine acoustique Ceased WO2007102713A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020060021976A KR100715157B1 (ko) 2006-03-08 2006-03-08 보이스코일모터 액츄에이터를 이용한 콤보 광학계 바이오칩검출 시스템
KR10-2006-0021976 2006-03-08

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WO2007102713A1 true WO2007102713A1 (fr) 2007-09-13

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014071253A1 (fr) * 2012-11-05 2014-05-08 California Institute Of Technology Instruments pour dispositifs biologiques de type échantillon-à-résultat
US8873055B2 (en) 2012-08-03 2014-10-28 California Institute Of Technology Optical technique for chemical and biochemical analysis
US8883088B2 (en) 2011-12-23 2014-11-11 California Institute Of Technology Sample preparation devices and systems
US8968585B2 (en) 2010-12-23 2015-03-03 California Institute Of Technology Methods of fabrication of cartridges for biological analysis
US8980550B2 (en) 2009-12-15 2015-03-17 California Institute Of Technology Methods for measuring samples using consumer electronic devices and systems
US9057568B2 (en) 2008-12-16 2015-06-16 California Institute Of Technology Temperature control devices and methods
US9090891B2 (en) 2011-12-23 2015-07-28 California Institute Of Technology Pen-shaped device for biological sample preparation and analysis
US9090890B2 (en) 2011-12-23 2015-07-28 California Institute Of Technology Devices and methods for biological sample preparation
US9233369B2 (en) 2010-12-23 2016-01-12 California Institute Of Technology Fluidic devices and fabrication methods for microfluidics
US9518291B2 (en) 2011-12-23 2016-12-13 California Institute Of Technology Devices and methods for biological sample-to-answer and analysis

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102126032B1 (ko) 2013-07-31 2020-07-08 삼성전자주식회사 다채널 형광 검출 모듈 및 이를 포함하는 핵산 분석 시스템
KR101774886B1 (ko) 2016-04-21 2017-09-19 주식회사 나노바이오라이프 자동초점 면역 크로마토그래피 고감도 검출 시스템

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JP2004144839A (ja) * 2002-10-22 2004-05-20 Olympus Corp 光走査装置
US6902112B2 (en) * 1999-05-24 2005-06-07 Agilent Technologies, Inc. Apparatus and method for scanning a surface

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KR19980053244A (ko) * 1996-12-26 1998-09-25 김영환 광 픽업(light pick-up) 장치

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US6902112B2 (en) * 1999-05-24 2005-06-07 Agilent Technologies, Inc. Apparatus and method for scanning a surface
JP2004144839A (ja) * 2002-10-22 2004-05-20 Olympus Corp 光走査装置

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9057568B2 (en) 2008-12-16 2015-06-16 California Institute Of Technology Temperature control devices and methods
US8980550B2 (en) 2009-12-15 2015-03-17 California Institute Of Technology Methods for measuring samples using consumer electronic devices and systems
US8968585B2 (en) 2010-12-23 2015-03-03 California Institute Of Technology Methods of fabrication of cartridges for biological analysis
US9233369B2 (en) 2010-12-23 2016-01-12 California Institute Of Technology Fluidic devices and fabrication methods for microfluidics
US8883088B2 (en) 2011-12-23 2014-11-11 California Institute Of Technology Sample preparation devices and systems
US9090891B2 (en) 2011-12-23 2015-07-28 California Institute Of Technology Pen-shaped device for biological sample preparation and analysis
US9090890B2 (en) 2011-12-23 2015-07-28 California Institute Of Technology Devices and methods for biological sample preparation
US9518291B2 (en) 2011-12-23 2016-12-13 California Institute Of Technology Devices and methods for biological sample-to-answer and analysis
US8873055B2 (en) 2012-08-03 2014-10-28 California Institute Of Technology Optical technique for chemical and biochemical analysis
WO2014071253A1 (fr) * 2012-11-05 2014-05-08 California Institute Of Technology Instruments pour dispositifs biologiques de type échantillon-à-résultat
US9284520B2 (en) 2012-11-05 2016-03-15 California Institute Of Technology Instruments for biological sample preparation devices
US9416343B2 (en) 2012-11-05 2016-08-16 California Institute Of Technology Instruments for biological sample-to-answer devices

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Publication number Publication date
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