WO2007107375A3 - Pipette en verre ou capillaire en verre pour expériences de patch-clamp - Google Patents

Pipette en verre ou capillaire en verre pour expériences de patch-clamp Download PDF

Info

Publication number
WO2007107375A3
WO2007107375A3 PCT/EP2007/002587 EP2007002587W WO2007107375A3 WO 2007107375 A3 WO2007107375 A3 WO 2007107375A3 EP 2007002587 W EP2007002587 W EP 2007002587W WO 2007107375 A3 WO2007107375 A3 WO 2007107375A3
Authority
WO
WIPO (PCT)
Prior art keywords
glass
patch clamp
pipette
capillary
clamp experiments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2007/002587
Other languages
German (de)
English (en)
Other versions
WO2007107375A2 (fr
Inventor
Albrecht Lepple-Wienhues
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Flyion GmbH
Original Assignee
Flyion GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Flyion GmbH filed Critical Flyion GmbH
Publication of WO2007107375A2 publication Critical patent/WO2007107375A2/fr
Publication of WO2007107375A3 publication Critical patent/WO2007107375A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne en premier lieu une pipette en verre ou un capillaire en verre (1) pour réaliser des expériences de patch-clamp, cette pipette ou ce capillaire présentant une pointe (2) conique et une section (3) sensiblement tubulaire adjacente à cette pointe (2). Ladite pipette ou ledit capillaire se caractérise en ce que son diamètre passe d'un diamètre D1 inférieur à 50 µm au niveau de la base de la pointe à un diamètre D2 supérieur ou égal à 100 µm dans la section tubulaire sur une longueur L inférieure à 150 µm. L'invention concerne en outre l'utilisation de cette pipette ou de ce capillaire pour réaliser des expériences de patch-clamp, ainsi que des mesures de patch-clamp au moyen de pipettes ou de capillaires de ce type.
PCT/EP2007/002587 2006-03-23 2007-03-23 Pipette en verre ou capillaire en verre pour expériences de patch-clamp Ceased WO2007107375A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200610014513 DE102006014513A1 (de) 2006-03-23 2006-03-23 Glaspipette oder Glaskapillare für Patch-Clamp-Experimente
DE102006014513.5 2006-03-23

Publications (2)

Publication Number Publication Date
WO2007107375A2 WO2007107375A2 (fr) 2007-09-27
WO2007107375A3 true WO2007107375A3 (fr) 2007-11-22

Family

ID=38426481

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/002587 Ceased WO2007107375A2 (fr) 2006-03-23 2007-03-23 Pipette en verre ou capillaire en verre pour expériences de patch-clamp

Country Status (2)

Country Link
DE (1) DE102006014513A1 (fr)
WO (1) WO2007107375A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2710109T3 (en) 2011-05-19 2018-01-22 Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Univ Tuebingen Method and use of a device for automated positioning of a microsystem for manipulating a spherical micro object

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001048475A1 (fr) * 1999-12-24 2001-07-05 Astrazeneca Ab Dispositif et procede permettant de faire des mesures electriques sur un objet
WO2002010747A2 (fr) * 2000-07-31 2002-02-07 Flyion Gmbh Technique et appareil patch-clamp permettant d'effectuer des mesures sur des cellules

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001048475A1 (fr) * 1999-12-24 2001-07-05 Astrazeneca Ab Dispositif et procede permettant de faire des mesures electriques sur un objet
WO2002010747A2 (fr) * 2000-07-31 2002-02-07 Flyion Gmbh Technique et appareil patch-clamp permettant d'effectuer des mesures sur des cellules

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HAMILL O P ET AL: "IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES", PFLUEGERS ARCHIV: EUROPEAN JOURNAL OF PHYSIOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 391, 1981, pages 85 - 100, XP000196663, ISSN: 0031-6768 *
NEHER E ET AL: "THE EXTRACELLULAR PATCH CLAMP: A METHOD FOR RESOLVING CURRENT THROUGH INDIVIDUAL OPEN CHANNELS IN BIOLOGICAL MEMBRANES", PFLUEGERS ARCHIV: EUROPEAN JOURNAL OF PHYSIOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 375, no. 2, 1978, pages 219 - 228, XP009075888, ISSN: 0031-6768 *

Also Published As

Publication number Publication date
WO2007107375A2 (fr) 2007-09-27
DE102006014513A1 (de) 2007-09-27

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