WO2007108689A3 - Nouveau procede d'induction de la differenciation de cellules souches et progenitrices - Google Patents

Nouveau procede d'induction de la differenciation de cellules souches et progenitrices Download PDF

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Publication number
WO2007108689A3
WO2007108689A3 PCT/NL2007/050120 NL2007050120W WO2007108689A3 WO 2007108689 A3 WO2007108689 A3 WO 2007108689A3 NL 2007050120 W NL2007050120 W NL 2007050120W WO 2007108689 A3 WO2007108689 A3 WO 2007108689A3
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WIPO (PCT)
Prior art keywords
cells
differentiation
stem cells
polyamine
combinations
Prior art date
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Ceased
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PCT/NL2007/050120
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English (en)
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WO2007108689A2 (fr
Inventor
Martinus Nicolaas Helder
Paulus Ignatius Jozef Wuisman
Doulabi Behrouz Zandieh
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STICHTING SKELETAL TISSUE ENGINEERING
STICHTING SKELETAL TISSUE ENGI
Original Assignee
STICHTING SKELETAL TISSUE ENGINEERING
STICHTING SKELETAL TISSUE ENGI
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Application filed by STICHTING SKELETAL TISSUE ENGINEERING, STICHTING SKELETAL TISSUE ENGI filed Critical STICHTING SKELETAL TISSUE ENGINEERING
Publication of WO2007108689A2 publication Critical patent/WO2007108689A2/fr
Publication of WO2007108689A3 publication Critical patent/WO2007108689A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1384—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from adipose-derived stem cells [ADSC], from adipose stromal stem cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

La présente invention concerne la propagation et la différenciation des cellules souches et les stimuli chimiques qui sont utilisés dans l'environnement de ces cellules pour induire la différenciation. Plus particulièrement, l'invention concerne un procédé d'induction ex vivo de la différenciation de cellules souches mésenchymateuses, dérivées de préférence de tissu adipeux, ou de cellules progénitrices en ajoutant une polyamine aux cellules pendant une courte durée (1 min - 2 heures), puis en l'éliminant. D'autres facteurs d'induction de la différenciation peuvent être ajoutés en parallèle, ces facteurs appartenant de préférence au groupe comrenant essentiellement des hormones stéroïdiennes, des vitamines et/ou des hormones de croissance, la LMP-I et leurs combinaisons. Le procédé décrit assure préférentiellement une différenciation ostéogénique ou chondrogénique et les cellules sont donc combinées de préférence à des matières bio-résorbables telles que des phosphates de calcium ostéo-conducteurs, des polymères bio-résorbables tels que des polylactides ou des polyglycolides, des poly-caprolactones, des collagènes, des minéraux, des fibrinogènes, des alginates, des hyaluronases, du chitosan et/ou leurs combinaisons. Les cellules souches et/ou les matières bio-résorbables seront soit implantées directement, soit davantage cultivées dans un milieu de culture approprié avant implantation. De plus, l'utilisation d'une polyamine est décrite en tant que procédé permettant d'éviter la dé-différenciation des cellules et/ou d'induire la trans-différenciation des cellules.
PCT/NL2007/050120 2006-03-21 2007-03-21 Nouveau procede d'induction de la differenciation de cellules souches et progenitrices Ceased WO2007108689A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06075658.2 2006-03-21
EP06075658 2006-03-21

Publications (2)

Publication Number Publication Date
WO2007108689A2 WO2007108689A2 (fr) 2007-09-27
WO2007108689A3 true WO2007108689A3 (fr) 2007-11-15

Family

ID=36843249

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2007/050120 Ceased WO2007108689A2 (fr) 2006-03-21 2007-03-21 Nouveau procede d'induction de la differenciation de cellules souches et progenitrices

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WO (1) WO2007108689A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104357381A (zh) * 2008-10-31 2015-02-18 辛西斯有限责任公司 用于活化干细胞的方法和装置
ES2370205B2 (es) 2010-05-18 2012-07-04 Universidad De Santiago De Compostela Uso de poloxaminas como inductoras de la diferenciacion osteogenica de celulas mesenquimales
WO2013162057A1 (fr) 2012-04-25 2013-10-31 独立行政法人理化学研究所 Préparation cellulaire contenant une cellule engagée du myocarde
CN104877962A (zh) * 2015-04-15 2015-09-02 广州赛莱拉干细胞科技股份有限公司 一种无血清的脂肪干细胞培养基及其制备方法
CN104877961A (zh) * 2015-04-15 2015-09-02 广州赛莱拉干细胞科技股份有限公司 一种无血清的人羊膜间充质干细胞培养基及其制备方法
CN104877963A (zh) * 2015-04-15 2015-09-02 广州赛莱拉干细胞科技股份有限公司 一种无血清的人脐带间充质干细胞培养基及其制备方法
JP2020525036A (ja) * 2017-06-29 2020-08-27 エッサー，クヌート 複数の未分化又は脱分化細胞、特に複数の固形腫瘍での（再）分化を誘導する複数の医薬薬剤の同定方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040866A1 (fr) * 1995-06-07 1996-12-19 Novartis Ag Milieux sans serum pour des cellules hematopoietiques primaires et procedes d'utilisation
WO2003011277A2 (fr) * 2001-07-31 2003-02-13 Anormed Inc. Methodes de mobilisation de cellules souches/embryonnaires
EP1332673A1 (fr) * 1998-02-17 2003-08-06 Gamida Cell Ltd. Procédé de contrôler la prolifération et la différentiation de cellules souches et progenitrices
WO2005034625A1 (fr) * 2003-09-12 2005-04-21 Becton, Dickinson And Company Collagene vi fixe par covalence pour fixation et proliferation de cellules

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040866A1 (fr) * 1995-06-07 1996-12-19 Novartis Ag Milieux sans serum pour des cellules hematopoietiques primaires et procedes d'utilisation
EP1332673A1 (fr) * 1998-02-17 2003-08-06 Gamida Cell Ltd. Procédé de contrôler la prolifération et la différentiation de cellules souches et progenitrices
WO2003011277A2 (fr) * 2001-07-31 2003-02-13 Anormed Inc. Methodes de mobilisation de cellules souches/embryonnaires
WO2005034625A1 (fr) * 2003-09-12 2005-04-21 Becton, Dickinson And Company Collagene vi fixe par covalence pour fixation et proliferation de cellules

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
AMEMIYA K ET AL: "Adult human retinal pigment epithelial cells capable of differentiating into neurons", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 316, no. 1, 26 March 2004 (2004-03-26), pages 1 - 5, XP004493132, ISSN: 0006-291X *
KNIPPENBERG ET AL: "Osteogenesis versus chondrogenesis by BMP-2 and BMP-7 in adipose stem cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 342, no. 3, 2006, pages 902 - 908, XP005312603, ISSN: 0006-291X *
MAEDA TOMOJI ET AL: "Role of polyamines derived from arginine in differentiation and proliferation of human blood cells.", BIOLOGICAL & PHARMACEUTICAL BULLETIN FEB 2006, vol. 29, no. 2, February 2006 (2006-02-01), pages 234 - 239, XP002445025, ISSN: 0918-6158 *
MURASHOV A K ET AL: "17beta-Estradiol enhances neuronal differentiation of mouse embryonic stem cells", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 569, no. 1-3, 2 July 2004 (2004-07-02), pages 165 - 168, XP004517478, ISSN: 0014-5793 *
TAKANO T ET AL: "Role of polyamines in expression of the differentiated phenotype of chondrocytes: effect of DL-alpha-hydrazino-delta-aminovaleric acid (DL-HAVA), an inhibitor of ornithine decarboxylase, on chondrocytes treated with parathyroid hormone.", JOURNAL OF BIOCHEMISTRY FEB 1983, vol. 93, no. 2, February 1983 (1983-02-01), pages 591 - 598, XP002445026, ISSN: 0021-924X *

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Publication number Publication date
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