WO2007136971A3 - Synergie enzyme-microbe - Google Patents

Synergie enzyme-microbe Download PDF

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Publication number
WO2007136971A3
WO2007136971A3 PCT/US2007/067954 US2007067954W WO2007136971A3 WO 2007136971 A3 WO2007136971 A3 WO 2007136971A3 US 2007067954 W US2007067954 W US 2007067954W WO 2007136971 A3 WO2007136971 A3 WO 2007136971A3
Authority
WO
WIPO (PCT)
Prior art keywords
cellulase
cellulose
cells
hydrolysis
reduced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/067954
Other languages
English (en)
Other versions
WO2007136971A2 (fr
Inventor
Lee R Lynd
Yi-Heng Percival Zhang
Yanpin Lu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dartmouth College
Original Assignee
Dartmouth College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dartmouth College filed Critical Dartmouth College
Priority to BRPI0711163-0A priority Critical patent/BRPI0711163A2/pt
Priority to AU2007253987A priority patent/AU2007253987A1/en
Priority to EP07811862A priority patent/EP2013355A2/fr
Priority to CN200780019672.5A priority patent/CN101466843B/zh
Priority to CA002651753A priority patent/CA2651753A1/fr
Priority to JP2009510037A priority patent/JP2009535067A/ja
Publication of WO2007136971A2 publication Critical patent/WO2007136971A2/fr
Publication of WO2007136971A3 publication Critical patent/WO2007136971A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Processing Of Solid Wastes (AREA)
  • Treatment Of Sludge (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés d'utilisation de charges de cellulase réduites pour hydrolyser des substrats cellulosiques. Les procédés comprennent la détermination d'une quantité de cellulase purifiée nécessaire pour sensiblement hydrolyser une quantité de substrat cellulosique pendant une période de temps; la réduction de la quantité de cellulase purifiée par un facteur d'entre 2 et 5 pour déterminer une charge de cellulase réduite; et l'introduction au substrat cellulosique soit (1) d'un microorganisme exprimant la cellulase liée à la cellule dans une concentration égale à la charge de cellulase réduite, soit (2) d'un agent de fermentation qui a été synthétisé pour exprimer la cellulase liée à la cellule dans une concentration égale à la charge de cellulase réduite dans des conditions appropriées et pendant ladite période de temps suffisante pour permettre une hydrolyse substantielle du substrat cellulosique.
PCT/US2007/067954 2006-05-01 2007-05-01 Synergie enzyme-microbe Ceased WO2007136971A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
BRPI0711163-0A BRPI0711163A2 (pt) 2006-05-01 2007-05-01 método de utilização de uma carga reduzida de celulase para hidrolisar um substrato celulósico
AU2007253987A AU2007253987A1 (en) 2006-05-01 2007-05-01 Process for the hydrolysis of cellulose mediated by ternary complexes of cellulose, clostridium thermocellum cells, and cellulase expressed by these cells
EP07811862A EP2013355A2 (fr) 2006-05-01 2007-05-01 Synergie enzyme-microbe
CN200780019672.5A CN101466843B (zh) 2006-05-01 2007-05-01 由纤维素、热纤梭菌细胞以及由这些细胞表达的纤维素酶形成的三元复合物介导的水解纤维素的方法
CA002651753A CA2651753A1 (fr) 2006-05-01 2007-05-01 Synergie enzyme-microbe
JP2009510037A JP2009535067A (ja) 2006-05-01 2007-05-01 セルロース、Ｃｌｏｓｔｒｉｄｉｕｍｔｈｅｒｍｏｃｅｌｌｕｍ細胞、およびこれらの細胞によって発現されたセルラーゼの三元複合体によって媒介されるセルロースの加水分解のためのプロセス

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79663506P 2006-05-01 2006-05-01
US60/796,635 2006-05-01

Publications (2)

Publication Number Publication Date
WO2007136971A2 WO2007136971A2 (fr) 2007-11-29
WO2007136971A3 true WO2007136971A3 (fr) 2008-04-10

Family

ID=38617943

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/067954 Ceased WO2007136971A2 (fr) 2006-05-01 2007-05-01 Synergie enzyme-microbe

Country Status (8)

Country Link
EP (1) EP2013355A2 (fr)
JP (1) JP2009535067A (fr)
CN (1) CN101466843B (fr)
AU (1) AU2007253987A1 (fr)
BR (1) BRPI0711163A2 (fr)
CA (1) CA2651753A1 (fr)
WO (1) WO2007136971A2 (fr)
ZA (1) ZA200809448B (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009033993A (ja) * 2007-07-31 2009-02-19 Toyota Central R&D Labs Inc セルラーゼ担持材料及びその利用
CA2724036C (fr) * 2008-05-11 2018-09-25 Mascoma Corporation Construction de souches de levures prototrophiques/cellulolytiques exprimant des cellulases ancrees et secretees
JP5352882B2 (ja) * 2009-12-04 2013-11-27 独立行政法人国際農林水産業研究センター クロストリジウム属微生物を利用したセルロース分解酵素の生産方法及びクロストリジウム属微生物の培養増殖方法。
CN103131733A (zh) * 2011-11-26 2013-06-05 嘉兴慧升生物技术咨询有限公司 一种利用生物废弃物组合生产生物质能的新方法
CN102643748A (zh) * 2012-05-09 2012-08-22 刘建伦 污水处理中污泥减量的微生物组合优势菌群
CN103688869B (zh) * 2013-01-22 2015-10-28 厦门嘉烨兴农业科技有限公司 一种用于养猪的发酵床
CN103664357B (zh) * 2013-11-26 2015-04-22 新疆晨源生物科技有限公司 一种有机发酵复合肥及其制备方法
CN104450829B (zh) * 2014-12-11 2018-06-12 中国科学院过程工程研究所 高温木质纤维素酶协同酶解制备可发酵糖的方法
CN108866025B (zh) * 2017-05-10 2021-04-13 中国科学院青岛生物能源与过程研究所 一种纤维素酶制剂及其应用
CN108866112B (zh) * 2018-08-17 2021-04-27 青岛中科潮生生物技术有限公司 采用木质纤维素制备角鲨烯的方法
CN108977421B (zh) * 2018-08-17 2021-04-13 中国科学院青岛生物能源与过程研究所 用于催化木质纤维素糖化的全菌酶制剂
CN109022506B (zh) * 2018-08-17 2021-04-27 青岛中科潮生生物技术有限公司 采用木质纤维素制备葡萄糖酸钠的方法
CN109019874B (zh) * 2018-09-08 2022-12-30 佛山市森昂生物科技有限公司 一种用于造纸废水的生物促生剂及其制备方法
CN110540982B (zh) * 2019-09-30 2021-11-02 江南大学 一种提高梭热杆菌纤维素酶酶活的发酵方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1065915C (zh) * 1991-03-18 2001-05-16 佛罗里达大学 通过重组宿主生产乙醇

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LU, Y.: "Enzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellum", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 103, no. 44, 31 October 2006 (2006-10-31), pages 16165 - 16169, XP002460702 *
LYND, L.R. ET AL.: "Microbial Cellulose Utilization: Fundamentals and Biotechnology", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 66, no. 3, September 2002 (2002-09-01), pages 506 - 577, XP002369450 *

Also Published As

Publication number Publication date
BRPI0711163A2 (pt) 2011-08-23
AU2007253987A1 (en) 2007-11-29
CN101466843B (zh) 2013-05-08
ZA200809448B (en) 2010-08-25
JP2009535067A (ja) 2009-10-01
EP2013355A2 (fr) 2009-01-14
WO2007136971A2 (fr) 2007-11-29
CA2651753A1 (fr) 2007-11-29
CN101466843A (zh) 2009-06-24

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WO2007136971A3 (fr) Synergie enzyme-microbe
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Tri et al. Butanol production from cellulosic material by anaerobic co-culture of white-rot fungus Phlebia and bacterium Clostridium in consolidated bioprocessing
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