WO2007142402A1 - testeur de fonctionNEMENT du foie et système d'essai de fonctionNEMENT du foie - Google Patents

testeur de fonctionNEMENT du foie et système d'essai de fonctionNEMENT du foie Download PDF

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Publication number
WO2007142402A1
WO2007142402A1 PCT/KR2007/001442 KR2007001442W WO2007142402A1 WO 2007142402 A1 WO2007142402 A1 WO 2007142402A1 KR 2007001442 W KR2007001442 W KR 2007001442W WO 2007142402 A1 WO2007142402 A1 WO 2007142402A1
Authority
WO
WIPO (PCT)
Prior art keywords
unit
tester
gpt
sensor strip
blood
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2007/001442
Other languages
English (en)
Inventor
Bong Hyun Chung
Joong Jin Lee
Sang Kyu Kim
Hye Jung Park
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Research Institute of Bioscience and Biotechnology KRIBB
Original Assignee
Korea Research Institute of Bioscience and Biotechnology KRIBB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020070006263A external-priority patent/KR20070117993A/ko
Application filed by Korea Research Institute of Bioscience and Biotechnology KRIBB filed Critical Korea Research Institute of Bioscience and Biotechnology KRIBB
Publication of WO2007142402A1 publication Critical patent/WO2007142402A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3273—Devices therefor, e.g. test element readers, circuitry

Definitions

  • the present invention relates, in general, to a liver function tester and liver function testing system and, in particular, to a liver function tester constituted as a portable module which simply makes liver measurements and displays the value through a mobile communication terminal or a computer connected to the tester, and a system comprising the same.
  • liver diseases are classified as viral liver diseases, alcoholic liver diseases caused by excessive drinking, toxic liver diseases caused by drugs, autoimmune liver diseases caused by improper functioning of the human body immune system, metabolic liver diseases, caused by excessive accumulation of toxic material, and liver diseases having unknown causes.
  • Liver disease means all diseases affecting the liver, and thus comprises hepatitis, cirrhosis and liver cancer. Hepatitis is classified as acute hepatitis, in which inflammation forms suddenly and usually disappears after 3 or 4 months, and chronic hepatitis, which lasts for longer than 6 months.
  • liver disease is the number 5 cause of death, and the number 1 cause among those in their 40s.
  • cirrhosis and liver cancer are one of the most important causes of death, and the incidence of liver cancer patients among middle aged (40-65 years old) people is 74.8 in 100,000 for men and 15.6 in 100,000 for women, which is the highest in the world.
  • Deaths attributable to liver cancer totaled 32.3 in 100,000 for men and 10.0 in 100,000 for women, which is the highest among 21 OECD countries that were compared, as reported in International Health Statistics Annual.
  • Typical factors for diagnosing liver disease are the enzymes Glutamate Pyruvic
  • liver condition can be determined according to increases and decreases in the levels of GPT and GOT in the blood.
  • the basic principle behind the conventional method for measuring liver parameters is the oxidizing enzyme-mediated oxidation of pyruvate and oxaloacetate, which are the products resulting from the enzymatic reaction of GPT and GOT with of L-alanine, L- aspartate, and L-ketoglutarate.
  • This method typically involves observing changes in the pigment of TMB as an indicator chromogen to measure the density of GPT and GOT.
  • This liver test method for GPT and GOP based on color change and comprising measuring the extent of color change, has a disadvantage in that it cannot use blood as it is in a quick manner, and requires blood serum. Furthermore, the apparatus for measuring the color change is too big to carry. Accordingly, people must travel to hospitals having the apparatus in order to obtain diagnoses, and therefore, they are reluctant to seek treatment. Disclosure of Invention Technical Problem
  • an object of the present invention is to provide a liver function tester which is constructed as a portable module so as to promptly and simply measure the level of GPT and GOT in the blood on the basis of electrochemical reactions.
  • Another object of the present invention is to provide a liver function testing system for measuring the level of GPT and GOT in which the tester can be connected to a mobile communication terminal or to a computer for display purposes, whereby it can measure level of GPT and GOT in real time to thus realize ubiquitous health care.
  • a liver function tester comprising; a sensor strip for converting density of GPT and GOP in the blood into current values; a current detection/amplification unit for detecting the current flowing in the sensor strip; and a control unit for converting the current into a digital signal and outputting the level of GPT and GOT from the digital signal.
  • the sensor strip comprises a reaction unit, in which amino acids reacting with GPT and GOT in blood and enzymes involved in the generation of electrons from the amino acids are fixed, and an electrode unit consisting of a plurality of working electrodes and a reference electrode, one end of each of the electrodes being connected to the reaction unit.
  • the amino acids fixed to the reaction unit may comprise L-alanine reacting with GPT and L-aspartate reacting with GOT.
  • the enzymes fixed on the reaction unit comprise L-glutamate oxidase, ⁇ -ketoglutaric acid and peroxidase.
  • reaction unit of the sensor strip may further comprise glucose oxidase, reacting with glucose, fixed thereon, whereby the tester can measure blood glucose levels.
  • the tester may further comprise a power supply unit for providing electric power to the electrode unit, the current detection/amplification unit and the control unit from an external mobile communication terminal or a computer connected thereto.
  • this tester may further comprise a transmitting/receiving unit for exchanging information on level of GPT and GOT with an external mobile communication terminal or a computer.
  • the sensor strip is detachably mounted on the tester so as to form one module with the other units of the tester.
  • a liver function testing system comprising; a tester for measuring the level of GPT and GOT in the blood; and a mobile communication terminal or computer which is connected to the tester so as to exchange signals therewith and has a display unit for displaying the measured levels of GPT and GOT thereon.
  • the tester comprises a sensor strip for converting the density of GPT and GOP in the blood into current values; a current detection/amplification unit for detecting current flowing in the sensor strip; a control unit, comprising an analog- digital converter for converting the detected current into a digital signal, adapted to outputting the digital signal; a power supply unit for providing electric power to the electrode unit, the current detection/amplification unit and the control unit from an external mobile communication terminal or a computer connected thereto; and a transmitting/receiving unit for receiving the digital signal from the control unit and transmitting it to the external mobile communication terminal or computer.
  • the sensor strip comprises: a reaction unit, in which amino acids reacting with GPT and GOT in blood and enzymes involved in the generation of electrons from the amino acids are fixed, and an electrode unit consisting of a plurality of working electrodes and a reference electrode, each of the electrodes being connected to the reaction unit at one end thereof and to the current detection/amplification unit at the other end thereof.
  • the reaction unit in the sensor strip further comprises glucose oxidase, which reacts with glucose, additionally fixed thereon, so that the system can also be used to measure blood glucose levels.
  • FlG. 1 is a structural view of an tester in accordance with an embodiment of the present invention.
  • FlG. 2 is an enlarged plan view of the sensor strip of FlG. 1 ;
  • FIGS. 3 to 5 are graphs showing the relationship between the current values detected by the tester 10 of the present invention and the concentrations(densities) of GPT, GOT and glucose in the blood, detected using a conventional electrochemical reaction measuring instrument;
  • FlG. 6 is an outlined structural view of a system in accordance with the embodiment of the present invention.
  • FlG. 7 is a block diagram showing the system of FlG. 6;
  • FlG. 8 is a view explaining the use of the system in accordance with the embodiment of the present invention. Best Mode for Carrying Out the Invention
  • a tester 10 is provided for measuring level of GPT and
  • the tester 10 comprises a sensor strip 20, a power supply unit 40, a current detection/ amplification unit 30, a control unit 50, and a transmitting/receiving unit 60.
  • the tester 10 is in the form of a portable module in which the sensor strip 20 is detachably mounted on the tester 10 for measuring levels of GPT and GOT.
  • the sensor strip 20 converts the density of GPT and GOT in the blood into electronic current values through an electrochemical method, and the tester 10 displays levels of GPT and GOT using the detected current value.
  • the present invention confers the advantage of enabling levels of GPT and GOT to be conveniently measured.
  • the structure of the sensor strip 20 is described in detail. As seen in this figure, it comprises a non-conductive substrate 21, a reaction unit 22, and an electrode unit 23 comprising a working electrode 24 and a reference electrode 25.
  • the tester 10 can also be adapted to measure blood sugar level.
  • glucose oxidase which reacts with glucose
  • glucose oxidase is fixed in the reaction unit 22 of the sensor strip 20.
  • glucose in the blood is oxidized while glucose oxidase is reduced.
  • an electron carrier transfers electrons from the reduced glucose oxidase to the working electrode 24, to which a predetermined potential is applied.
  • the electron carrier receives electrons from the reduced glucose oxidase so that a redox reaction occurs to reduce the electron carrier while the reduced glucose oxidase reverts to neutral. Then, the electron carrier loses the electrons at the working electrode 24. During the electron transfer, current is therefore generated in proportion with the level of blood sugar.
  • the electrode unit 23 comprises the working electrode 24 and the reference electrode 25.
  • the working electrode 24 and the reference electrode 25 are separately connected to the reaction unit 22 at their respective ends, so that the current (electrons) generated as a result of the reaction of GPT and GOT in the blood sample interacting with the amino acids in association with the fixed enzymes in the reaction unit 22 flows through the electrode unit 23.
  • three working electrodes are provided to measure GPT, GOT and glucose.
  • the procedure for measuring GPT and GOT is described in detail using reaction formulas.
  • As for the measurement of glucose its description is omitted because it is commonly known.
  • Three working electrodes 24a, 24b and 24c of the electrode unit 23 are adapted to measure levels of GPT, GOT and glucose in the blood, respectively.
  • proper reaction materials are fixed to the working electrodes of the reaction unit 22.
  • L-alanine for example, is fixed to the first working electrode 24a for measuring GPT, L-aspartate to the second working electrode 24b for measuring GOT, and glucose oxidase to the third working electrode 24c for measuring glucose.
  • a mixture fluid of ⁇ -ketoglutaric acid, L-glutamate oxidase and peroxidase is fixed, along with the amino acid substrates, to both the first and the second working electrodes 24a, 24b.
  • the power supply unit 40 provides electricity to the electrode unit 23, the current detection/amplification unit 30, and the control unit 50. Electric power may be supplied to the power supply unit 40 from a mobile communication terminal 110 or a computer 120 (F1G.6), as will be described later, when the tester 10 is connected to them.
  • the power supply unit 40 can employ a direct current voltage source when connected to a mobile communication terminal 110, or either a direct current or alternating current voltage source when connected to a computer 120.
  • the current detection/amplification unit 30 functions to detect the quantity of current flowing between the working electrode 24 and the reference electrode 25 of the electrode unit 23. To accomplish this, the current detection/amplification unit 30 is connected to the other end of the working electrode 24 and the reference electrode 25.
  • three switches SWl, SW2 and SW3 are provided, which connect the three working electrodes to three resistors Rl, R2 and R3, respectively.
  • the three switches SWl, SW2 and SW3 are successively operated in such a way that one is in an ON state while the others are in an OFF state. For example, when SWl is on, SW2 and SW3 are both off.
  • Current flowing through the working electrodes is converted to voltage by the resistors Rl, R2 and R3 in association with respective operational amplifiers OPl, OP2 and OP3. The converted voltage is input into the control unit 50.
  • the control unit 50 converts the voltages input from the current detection/amplification unit 30 into digital signals using analog-digital converters ADCl, ADC2 and ADC3. These digital signals are used to output level of GPT , GOT and blood sugar information.
  • the control unit 50 can output level information by converting the quantity of current flowing in each working electrode 24 into a voltage value.
  • the transmitting/receiving unit 60 receives the converted level information from the control unit 50 and transmits it to the mobile communication terminal 110 or the computer 120 in which the information is read.
  • the information exchange of the transmitting/receiving unit 60 may be performed in a wireless manner.
  • the most important characteristic of the present invention is that an electrochemical method, currently used to measure blood sugar level, is also used to make liver function measurements. Therefore, the tester according to the present invention can be manufactured as a portable module which makes it convenient for users to simply and promptly test liver function. In addition, the tester according to the present invention confers the advantage of measuring blood glucose level at the same time.
  • FIGS. 3 to 5 are graphs showing the relationship between the current values detected by the tester 10 and the concentrations(densities) of GPT, GOT and glucose in the blood, detected using a conventional electrochemical reaction measuring instrument.
  • a system 100 for testing liver functions.
  • the system 100 comprises the tester 10 and a mobile communication terminal 110, such as a cellular phone or a PDA, or a computer 120, whether a laptop or desktop.
  • a mobile communication terminal 110 such as a cellular phone or a PDA, or a computer 120, whether a laptop or desktop.
  • the mobile communication terminal 110 or the computer 120 has a transmitting/ receiving unit 111 or 121 provided to receive the information from the tester 10 through an interface (not shown) such as a USB or RS-232C port, or in a wireless manner, such as such as IrDA or Blue Tooth.
  • the control unit 112 of the mobile communication terminal/control unit 122 of the computer After converting level information transmitted from the tester 10 into a digital signal, the control unit 112 of the mobile communication terminal/control unit 122 of the computer displays said information on each display unit 113, 123. They also perform control to drive sound process unit 114, 124 to audibly inform the blind or the old of the liver measurements. Preferably, the sound process unit 114, 124 generates a warning sound when the levels are dangerously high, that is, exceeding a certain level.
  • Each key operation unit 115, 125 can store information about liver measurement diagnoses, transmitted from the tester 10, in a database.
  • Each data base 116, 126 stores a list of users for whom liver function test have been taken and the levels in a table form, or sequentially according to date. Thus, liver function measurements are stored in an orderly manner according to user or date, and can be searched systemically.
  • the tester 10 based on an electrochemical method, in accordance with the present invention is composed of a portable module so that it allows the user to promptly and simply measure the level of GPT and GOT in the blood and can display the levels in real time when connected to a mobile communication terminal or to a computer in the system 100 of the present invention.
  • the tester and the system have the advantage of realizing ubiquitous health care.
  • the mobile communication terminal 110 or computer 120 provides the liver function information via wired or wireless communication after connecting with a web server of management service provider 130 or a server of a competent hospital 140, as set by the user.
  • the service provider 130 or the competent hospital 140 can provide prescription information, dietetic therapy information and exercise therapy information in order to manage liver function. They also can store and receive a patient's medical examination information and can inform the patient of the process via an SMS sent to the patient's cellular ph one.
  • the web server of management service provider 130 or the competent hospital 140 in which liver measurements of each patient are stored in a database, may provide the user with information on liver function or abnormal changes in order to promptly manage liver functionss, thereby preventing unexpected problems in advance.
  • a patient can be continuously provided with liver function management in real time. There is an advantage in that the patient does not need to frequently go to the hospital in order to undergo liver examination.
  • the tester and system according to the present invention can promptly and simply measure the level of GPT and GOT in the blood on the basis of an electrochemical method. Thanks to the constitution as a portable module, the tester of the present invention makes it possible for the user to measure levels of GPT and GPT quickly and readily. The tester is also convenient because the liver measurements can be displayed on a mobile communication terminal or a computer via wired or wireless communication therewith.
  • a user can be continuously provided with management of liver function in remote places, and the inconvenience of having to go to the hospital to examine and treat the liver is minimized.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Electrochemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un testeur de fonctionnement du foie et un système d'essai de fonctionnement du foie. Le testeur comprend une bande capteur permettant de convertir la densité de GPT et GOP dans le sang en valeurs de courant, une unité de détection/amplification de courant permettant de détecter le courant s'écoulant dans la bande capteur, et une unité de commande permettant de convertir le courant en signal numérique et de générer le niveau de GPT et GOP à partir du signal numérique. La bande capteur comprend une unité de réaction, dans laquelle les acides aminés, réagissant avec le GPT et le GOT dans le sang, et les enzymes, intervenant dans la génération d'électrons provenant des acides aminés, sont fixés, et une unité d'électrode consistant en une pluralité d'électrodes de travail et une électrode de référence, une extrémité de chacune desdites électrodes étant connectée à l'unité de réaction.
PCT/KR2007/001442 2006-06-09 2007-03-23 testeur de fonctionNEMENT du foie et système d'essai de fonctionNEMENT du foie Ceased WO2007142402A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20060052119 2006-06-09
KR10-2006-0052119 2006-06-09
KR1020070006263A KR20070117993A (ko) 2006-06-09 2007-01-19 간 수치 측정 장치 및 간 수치 측정 시스템
KR10-2007-0006263 2007-01-19

Publications (1)

Publication Number Publication Date
WO2007142402A1 true WO2007142402A1 (fr) 2007-12-13

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PCT/KR2007/001442 Ceased WO2007142402A1 (fr) 2006-06-09 2007-03-23 testeur de fonctionNEMENT du foie et système d'essai de fonctionNEMENT du foie

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5496453A (en) * 1991-05-17 1996-03-05 Kyoto Daiichi Kagaku Co., Ltd. Biosensor and method of quantitative analysis using the same
US5695623A (en) * 1989-07-07 1997-12-09 Disetronic Licensing Ag Glucose measuring device
US5705045A (en) * 1995-08-29 1998-01-06 Lg Electronics Inc. Multi-biosensor for GPT and got activity
US5798031A (en) * 1997-05-12 1998-08-25 Bayer Corporation Electrochemical biosensor
US6475750B1 (en) * 1999-05-11 2002-11-05 M-Biotech, Inc. Glucose biosensor
US7041468B2 (en) * 2001-04-02 2006-05-09 Therasense, Inc. Blood glucose tracking apparatus and methods

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5695623A (en) * 1989-07-07 1997-12-09 Disetronic Licensing Ag Glucose measuring device
US5496453A (en) * 1991-05-17 1996-03-05 Kyoto Daiichi Kagaku Co., Ltd. Biosensor and method of quantitative analysis using the same
US5705045A (en) * 1995-08-29 1998-01-06 Lg Electronics Inc. Multi-biosensor for GPT and got activity
US5798031A (en) * 1997-05-12 1998-08-25 Bayer Corporation Electrochemical biosensor
US6475750B1 (en) * 1999-05-11 2002-11-05 M-Biotech, Inc. Glucose biosensor
US7041468B2 (en) * 2001-04-02 2006-05-09 Therasense, Inc. Blood glucose tracking apparatus and methods

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