WO2008093010A3 - Procede de diversification aleatoire d'une sequence genetique permettant de preserver l'identite de certains segments internes de ladite sequence genetique - Google Patents

Procede de diversification aleatoire d'une sequence genetique permettant de preserver l'identite de certains segments internes de ladite sequence genetique Download PDF

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Publication number
WO2008093010A3
WO2008093010A3 PCT/FR2007/002102 FR2007002102W WO2008093010A3 WO 2008093010 A3 WO2008093010 A3 WO 2008093010A3 FR 2007002102 W FR2007002102 W FR 2007002102W WO 2008093010 A3 WO2008093010 A3 WO 2008093010A3
Authority
WO
WIPO (PCT)
Prior art keywords
genetic sequence
preserving
identity
inner segments
diversification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2007/002102
Other languages
English (en)
Other versions
WO2008093010A2 (fr
WO2008093010A8 (fr
Inventor
Francois Iris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Phaxiam Therapeutics SA
Original Assignee
Pherecydes Pharma SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pherecydes Pharma SA filed Critical Pherecydes Pharma SA
Priority to AU2007346007A priority Critical patent/AU2007346007B2/en
Priority to US12/528,257 priority patent/US8728734B2/en
Priority to EP07872389.7A priority patent/EP2097524B1/fr
Priority to ES07872389.7T priority patent/ES2582385T3/es
Priority to CA2673004A priority patent/CA2673004C/fr
Priority to JP2009542134A priority patent/JP5622394B2/ja
Publication of WO2008093010A2 publication Critical patent/WO2008093010A2/fr
Publication of WO2008093010A3 publication Critical patent/WO2008093010A3/fr
Anticipated expiration legal-status Critical
Publication of WO2008093010A8 publication Critical patent/WO2008093010A8/fr
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention vise un procédé très général pour diversifier de manière aléatoire une séquence nucléotidique S par PCR, tout en préservant l'identité de certains domaines de ladite séquence S, une banque de séquences nucléotidiques ainsi diversifiées, les protéines diversifiées obtenues par expressions de ces séquences nucléotidiques dans un hôte approprié.
PCT/FR2007/002102 2006-12-20 2007-12-18 Procede de diversification aleatoire d'une sequence genetique permettant de preserver l'identite de certains segments internes de ladite sequence genetique Ceased WO2008093010A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2007346007A AU2007346007B2 (en) 2006-12-20 2007-12-18 Method for the random diversification of a genetic sequence while preserving the identity of some inner segments of said genetic sequence
US12/528,257 US8728734B2 (en) 2006-12-20 2007-12-18 Method for the random diversification of a genetic sequence while preserving the identity of some inner segments of said genetic sequence
EP07872389.7A EP2097524B1 (fr) 2006-12-20 2007-12-18 Procede de diversification aleatoire d'une sequence genetique permettant de preserver l'identite de certains segments internes de ladite sequence genetique
ES07872389.7T ES2582385T3 (es) 2006-12-20 2007-12-18 Método de diversificación aleatoria de una secuencia genética que permite preservar la identidad de determinados segmentos internos de dicha secuencia genética
CA2673004A CA2673004C (fr) 2006-12-20 2007-12-18 Procede de diversification aleatoire d'une sequence genetique permettant de preserver l'identite de certains segments internes de ladite sequence genetique
JP2009542134A JP5622394B2 (ja) 2006-12-20 2007-12-18 遺伝子配列を前記遺伝子配列の所定の内部セグメントの一致度を維持しながらランダムに多様化する方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0655723A FR2910493B1 (fr) 2006-12-20 2006-12-20 Procede de diversification aleatoire d'une sequence genetique permettant de preserver l'identite de certains segments internes de ladite sequence genetique
FR0655723 2006-12-20

Publications (3)

Publication Number Publication Date
WO2008093010A2 WO2008093010A2 (fr) 2008-08-07
WO2008093010A3 true WO2008093010A3 (fr) 2008-12-31
WO2008093010A8 WO2008093010A8 (fr) 2009-07-16

Family

ID=38337662

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2007/002102 Ceased WO2008093010A2 (fr) 2006-12-20 2007-12-18 Procede de diversification aleatoire d'une sequence genetique permettant de preserver l'identite de certains segments internes de ladite sequence genetique

Country Status (8)

Country Link
US (1) US8728734B2 (fr)
EP (1) EP2097524B1 (fr)
JP (1) JP5622394B2 (fr)
AU (1) AU2007346007B2 (fr)
CA (1) CA2673004C (fr)
ES (1) ES2582385T3 (fr)
FR (1) FR2910493B1 (fr)
WO (1) WO2008093010A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2945049B1 (fr) 2009-04-30 2013-10-04 Pherecydes Pharma Modification du genome d'un bacteriophage lytique par immobilisation dudit bacteriophage dans sa bacterie hote

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998016661A2 (fr) * 1996-10-17 1998-04-23 Morphagen Morphatides: nouvelles banques de formes et de structures
WO1999023107A1 (fr) * 1997-10-31 1999-05-14 Maxygen, Incorporated Modification du tropisme viral et de la diversite d'especes hote par recombinaison du genome viral
WO2002007742A2 (fr) * 2000-07-25 2002-01-31 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Bacteriophage a spectre d'activite multiple
WO2006066224A2 (fr) * 2004-12-14 2006-06-22 Yale University Antibiotiques ciblés contre un facteur de virulence
EP1696037A2 (fr) * 1999-05-05 2006-08-30 Phylogica Limited Isolation de modulateurs biologiques à partir de banques de fragments de gènes présentant une diversité biologique

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6410271B1 (en) * 2000-06-23 2002-06-25 Genetastix Corporation Generation of highly diverse library of expression vectors via homologous recombination in yeast

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998016661A2 (fr) * 1996-10-17 1998-04-23 Morphagen Morphatides: nouvelles banques de formes et de structures
WO1999023107A1 (fr) * 1997-10-31 1999-05-14 Maxygen, Incorporated Modification du tropisme viral et de la diversite d'especes hote par recombinaison du genome viral
EP1696037A2 (fr) * 1999-05-05 2006-08-30 Phylogica Limited Isolation de modulateurs biologiques à partir de banques de fragments de gènes présentant une diversité biologique
WO2002007742A2 (fr) * 2000-07-25 2002-01-31 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Bacteriophage a spectre d'activite multiple
WO2006066224A2 (fr) * 2004-12-14 2006-06-22 Yale University Antibiotiques ciblés contre un facteur de virulence

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PATTEN P A ET AL: "APPLICATIONS OF DNA SHUFFLING TO PHARMACEUTICALS AND VACCINES", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 8, 1997, pages 724 - 733, XP002916609, ISSN: 0958-1669 *
YOICHI M ET AL: "Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157:H7", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 115, no. 1, 12 January 2005 (2005-01-12), pages 101 - 107, XP004966993, ISSN: 0168-1656 *

Also Published As

Publication number Publication date
EP2097524B1 (fr) 2016-04-13
JP5622394B2 (ja) 2014-11-12
US20110053801A1 (en) 2011-03-03
JP2010512775A (ja) 2010-04-30
ES2582385T3 (es) 2016-09-12
EP2097524A2 (fr) 2009-09-09
CA2673004C (fr) 2017-11-21
AU2007346007B2 (en) 2013-09-05
US8728734B2 (en) 2014-05-20
FR2910493A1 (fr) 2008-06-27
CA2673004A1 (fr) 2008-08-07
WO2008093010A2 (fr) 2008-08-07
FR2910493B1 (fr) 2012-12-14
WO2008093010A8 (fr) 2009-07-16
AU2007346007A1 (en) 2008-08-07

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