WO2008105513A1 - 多剤排出蛋白質欠損株由来の形質転換株およびそれらを用いた微生物変換方法 - Google Patents

多剤排出蛋白質欠損株由来の形質転換株およびそれらを用いた微生物変換方法 Download PDF

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Publication number
WO2008105513A1
WO2008105513A1 PCT/JP2008/053565 JP2008053565W WO2008105513A1 WO 2008105513 A1 WO2008105513 A1 WO 2008105513A1 JP 2008053565 W JP2008053565 W JP 2008053565W WO 2008105513 A1 WO2008105513 A1 WO 2008105513A1
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Prior art keywords
efflux protein
multidrug efflux
strain
same
transformant
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Ceased
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PCT/JP2008/053565
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English (en)
French (fr)
Inventor
Tadashi Fujii
Atsushi Ochiai
Shoji Ito
Hiroki Kabumoto
Yoshikazu Fujii
Kazuhiro Machida
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Mercian Corp
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Mercian Corp
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Priority to CN2008800067451A priority Critical patent/CN101675157B/zh
Priority to AU2008219996A priority patent/AU2008219996B2/en
Priority to JP2009501308A priority patent/JP5646168B2/ja
Priority to EP08721014.2A priority patent/EP2130907B1/en
Priority to ES08721014T priority patent/ES2426640T3/es
Priority to CA2679532A priority patent/CA2679532C/en
Priority to US12/449,868 priority patent/US8735135B2/en
Publication of WO2008105513A1 publication Critical patent/WO2008105513A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

異種生物由来の遺伝子を組み込んだ形質転換体を用いた現状の微生物変換においてみられる変換効率の低さを改善する手段の提供を目的とする。多剤排出蛋白質をコードする遺伝子を欠損した微生物を宿主として用い、これに異種生物由来の遺伝子を組み込んだ形質転換体を作製し、これを微生物変換に使用することにより、疎水性または両親媒性の基質化合物を非常に効率よくその目的化合物に変換することができる。大腸菌を宿主とする場合、欠損させる多剤排出蛋白質をコードする遺伝子としては、tolC、acrA、acrB等が挙げられる。
PCT/JP2008/053565 2007-03-01 2008-02-28 多剤排出蛋白質欠損株由来の形質転換株およびそれらを用いた微生物変換方法 Ceased WO2008105513A1 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CN2008800067451A CN101675157B (zh) 2007-03-01 2008-02-28 来自多药运出蛋白缺损株的转化株以及使用该转化株的微生物转化方法
AU2008219996A AU2008219996B2 (en) 2007-03-01 2008-02-28 Transformed strains originated from multidrug efflux protein defective strains and a method for microbial conversion using them
JP2009501308A JP5646168B2 (ja) 2007-03-01 2008-02-28 多剤排出蛋白質欠損株由来の形質転換株およびそれらを用いた微生物変換方法
EP08721014.2A EP2130907B1 (en) 2007-03-01 2008-02-28 Transformed strain derived from strain deficient in multidrug efflux protein, and bioconversion method using the same
ES08721014T ES2426640T3 (es) 2007-03-01 2008-02-28 Cepa transformada derivada de una cepa deficiente en proteína de eflujo multifármaco y método de bioconversión utilizando la misma
CA2679532A CA2679532C (en) 2007-03-01 2008-02-28 Transformed strains originated from multidrug efflux protein defective strains and a method for microbial conversion using them
US12/449,868 US8735135B2 (en) 2007-03-01 2008-02-28 Transformed strains originated from multidrug efflux protein defective strains and a method for microbial conversion using them

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-050935 2007-03-01
JP2007050935 2007-03-01

Publications (1)

Publication Number Publication Date
WO2008105513A1 true WO2008105513A1 (ja) 2008-09-04

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Application Number Title Priority Date Filing Date
PCT/JP2008/053565 Ceased WO2008105513A1 (ja) 2007-03-01 2008-02-28 多剤排出蛋白質欠損株由来の形質転換株およびそれらを用いた微生物変換方法

Country Status (8)

Country Link
US (1) US8735135B2 (ja)
EP (1) EP2130907B1 (ja)
JP (1) JP5646168B2 (ja)
CN (1) CN101675157B (ja)
AU (1) AU2008219996B2 (ja)
CA (1) CA2679532C (ja)
ES (1) ES2426640T3 (ja)
WO (1) WO2008105513A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012512648A (ja) * 2008-12-19 2012-06-07 イルドン ファーマ カンパニー リミテッド カルシトリオール又はカルシフェジオールの生産促進用バッファ組成物及びこれを利用したカルシトリオール又はカルシフェジオール製産方法

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Publication number Priority date Publication date Assignee Title
CN103898071B (zh) * 2012-12-24 2017-04-12 清华大学 P450sca‑2酶突变体、其制备方法和应用
AU2014241191A1 (en) 2013-03-27 2015-10-22 Sample Technologies, Inc. Recombinant phage and bacterial detection methods
AU2014281406A1 (en) * 2013-06-19 2016-01-21 Sample6 Technologies, Inc. Phage-based bacterial detection assay
JP7046349B2 (ja) * 2016-01-06 2022-04-04 学校法人沖縄科学技術大学院大学学園 加水分解活性を示す新規ペプチドおよびその用途
KR102251947B1 (ko) * 2019-10-31 2021-05-17 대상 주식회사 mdtK 유전자 불활성에 의해 방향족 아미노산 생산능력이 향상된 균주
CN113583983B (zh) * 2020-04-30 2025-05-02 弈柯莱生物科技(集团)股份有限公司 一种融合蛋白或其变体及其在制备骨化二醇中的应用
CN116790393B (zh) * 2023-06-21 2024-05-31 江南大学 一种改造酿酒酵母菌以葡萄糖为底物合成活性vd3的方法
CN121495951A (zh) * 2024-12-13 2026-02-10 广东省科学院微生物研究所(广东省微生物分析检测中心) 外排蛋白泵基因簇在提高菌株耐受tbbpa中的应用

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012512648A (ja) * 2008-12-19 2012-06-07 イルドン ファーマ カンパニー リミテッド カルシトリオール又はカルシフェジオールの生産促進用バッファ組成物及びこれを利用したカルシトリオール又はカルシフェジオール製産方法

Also Published As

Publication number Publication date
CA2679532C (en) 2016-07-26
US8735135B2 (en) 2014-05-27
AU2008219996B2 (en) 2013-09-12
EP2130907A4 (en) 2010-05-05
US20120040441A1 (en) 2012-02-16
JPWO2008105513A1 (ja) 2010-06-03
EP2130907A1 (en) 2009-12-09
CA2679532A1 (en) 2008-09-04
JP5646168B2 (ja) 2014-12-24
CN101675157B (zh) 2013-07-17
CN101675157A (zh) 2010-03-17
EP2130907B1 (en) 2013-06-12
ES2426640T3 (es) 2013-10-24
AU2008219996A1 (en) 2008-09-04

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