WO2008156021A1 - 細胞壁を備える細胞に外来物質を導入する方法 - Google Patents

細胞壁を備える細胞に外来物質を導入する方法 Download PDF

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Publication number
WO2008156021A1
WO2008156021A1 PCT/JP2008/060689 JP2008060689W WO2008156021A1 WO 2008156021 A1 WO2008156021 A1 WO 2008156021A1 JP 2008060689 W JP2008060689 W JP 2008060689W WO 2008156021 A1 WO2008156021 A1 WO 2008156021A1
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WO
WIPO (PCT)
Prior art keywords
cell
foreign substance
cell wall
carbon nanotube
introducing foreign
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/060689
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English (en)
French (fr)
Inventor
Yoshinobu Baba
Manabu Tokeshi
Noritada Kaji
Ibrahim Maged Fouad Serag Eldin Bayoumi
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Nagoya University NUC
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Nagoya University NUC
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Filing date
Publication date
Application filed by Nagoya University NUC filed Critical Nagoya University NUC
Priority to CN2008800211823A priority Critical patent/CN101688218B/zh
Priority to AU2008264598A priority patent/AU2008264598B2/en
Priority to JP2009520446A priority patent/JP4469959B2/ja
Priority to US12/665,186 priority patent/US8420891B2/en
Priority to EP08765468.7A priority patent/EP2169070B1/en
Publication of WO2008156021A1 publication Critical patent/WO2008156021A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

　本発明の方法は、細胞壁を備える細胞（１）に外来物質（３）を導入する方法である。この方法は、少なくとも１種の細胞壁分解酵素を担持したカーボンナノチューブ（２）を用意すること、前記細胞（１）を含む処理液中に細胞壁分解酵素担持カーボンナノチューブ（２）及び導入対象である外来物質（３）を供給すること、前記細胞（１）に接触したカーボンナノチューブ（２）の有する細胞壁分解酵素の作用によって該細胞（１）の細胞壁を分解すること、および、細胞壁が分解された部位より細胞（１）内に目的の外来物質（３）が導入されることを包含する。
PCT/JP2008/060689 2007-06-21 2008-06-11 細胞壁を備える細胞に外来物質を導入する方法 Ceased WO2008156021A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN2008800211823A CN101688218B (zh) 2007-06-21 2008-06-11 向具有细胞壁的细胞导入外来物质的方法
AU2008264598A AU2008264598B2 (en) 2007-06-21 2008-06-11 Method for introducing foreign substance into cell having cell wall
JP2009520446A JP4469959B2 (ja) 2007-06-21 2008-06-11 細胞壁を備える細胞に外来物質を導入する方法
US12/665,186 US8420891B2 (en) 2007-06-21 2008-06-11 Method for introducing foreign substance into cell having cell wall
EP08765468.7A EP2169070B1 (en) 2007-06-21 2008-06-11 Method for introducing foreign substance into cell having cell wall

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007163867 2007-06-21
JP2007-163867 2007-06-21

Publications (1)

Publication Number Publication Date
WO2008156021A1 true WO2008156021A1 (ja) 2008-12-24

Family

ID=40156178

Family Applications (1)

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PCT/JP2008/060689 Ceased WO2008156021A1 (ja) 2007-06-21 2008-06-11 細胞壁を備える細胞に外来物質を導入する方法

Country Status (6)

Country Link
US (1) US8420891B2 (ja)
EP (1) EP2169070B1 (ja)
JP (1) JP4469959B2 (ja)
CN (1) CN101688218B (ja)
AU (1) AU2008264598B2 (ja)
WO (1) WO2008156021A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8614366B2 (en) 2009-09-06 2013-12-24 Stephen R. Wilson Methods for genetic plant transformation using water-soluble fullerene derivatives

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US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
EP2755466A4 (en) 2011-09-13 2015-04-15 Monsanto Technology Llc METHOD AND COMPOSITIONS FOR WEED CONTROL
ES2764105T3 (es) 2011-10-17 2020-06-02 Massachusetts Inst Technology Administración intracelular
US10240162B2 (en) 2012-05-24 2019-03-26 A.B. Seeds Ltd. Compositions and methods for silencing gene expression
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
WO2014164761A1 (en) 2013-03-13 2014-10-09 Monsanto Technology Llc Methods and compositions for weed control
EP2967082A4 (en) 2013-03-13 2016-11-02 Monsanto Technology Llc METHOD AND COMPOSITIONS FOR WEED CONTROL
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
JP6668236B2 (ja) 2013-07-19 2020-03-18 モンサント テクノロジー エルエルシー Ｌｅｐｔｉｎｏｔａｒｓａ防除用組成物及びその方法
SG10201801188XA (en) 2013-08-16 2018-03-28 Massachusetts Inst Technology Selective delivery of material to cells
MX390055B (es) 2013-11-04 2025-03-20 Monsanto Technology Llc Composiciones y metodos para controlar infestaciones de plagas y parasitos de los artropodos.
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
WO2015200223A1 (en) 2014-06-23 2015-12-30 Monsanto Technology Llc Compositions and methods for regulating gene expression via rna interference
US11807857B2 (en) 2014-06-25 2023-11-07 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
AU2015296700B2 (en) 2014-07-29 2021-10-21 Monsanto Technology Llc Compositions and methods for controlling insect pests
RU2739794C2 (ru) 2014-10-31 2020-12-28 Массачусетс Инститьют Оф Текнолоджи Доставка биомолекул в клетки иммунной системы
JP6846345B2 (ja) 2014-11-14 2021-03-24 マサチューセッツ インスティテュート オブ テクノロジー 破壊及び場による細胞への化合物及び組成物の送達
AU2016206870B2 (en) 2015-01-12 2022-02-17 Massachusetts Institute Of Technology Gene editing through microfluidic delivery
US10968449B2 (en) 2015-01-22 2021-04-06 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
US10883103B2 (en) 2015-06-02 2021-01-05 Monsanto Technology Llc Compositions and methods for delivery of a polynucleotide into a plant
EP3302030A4 (en) 2015-06-03 2019-04-24 Monsanto Technology LLC METHOD AND COMPOSITIONS FOR INTRODUCING NUCLEIC ACIDS IN PLANTS
AU2016289530B2 (en) 2015-07-09 2021-05-06 Massachusetts Institute Of Technology Delivery of materials to anucleate cells
WO2017025967A1 (en) * 2015-08-13 2017-02-16 Forrest Innovations Ltd. Formulations and compositions for delivery of nucleic acids to plant cells
WO2017041051A1 (en) * 2015-09-04 2017-03-09 Sqz Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
CN112284861B (zh) * 2020-10-21 2023-02-17 上海市农业科学院 用于真姬菇担孢子显微观察的固定液、制备方法、固定方法及应用

Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2002325572A (ja) 2000-12-25 2002-11-12 Univ Osaka 外来物質の導入方法
WO2006130150A2 (en) * 2004-06-23 2006-12-07 Hyperion Catalysis International, Inc. Functionalized single walled carbon nanotubes
JP2007163867A (ja) 2005-12-14 2007-06-28 Fujitsu Ltd フォトマスクの製造方法、デバイスの製造方法、及び、フォトマスクのモニタ方法

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US20080014471A1 (en) * 2006-07-13 2008-01-17 Battelle Memorial Institute Biomolecular hybrid material and process for preparing same and uses for same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002325572A (ja) 2000-12-25 2002-11-12 Univ Osaka 外来物質の導入方法
WO2006130150A2 (en) * 2004-06-23 2006-12-07 Hyperion Catalysis International, Inc. Functionalized single walled carbon nanotubes
JP2007163867A (ja) 2005-12-14 2007-06-28 Fujitsu Ltd フォトマスクの製造方法、デバイスの製造方法、及び、フォトマスクのモニタ方法

Non-Patent Citations (3)

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Title
KLUMPP C. ET AL.: "Functionalized carbon nanotubes as emerging nanovectors for the delivery of therapeutics", BIOCHIM. BIOPHYS. ACTA, vol. 1758, 2006, pages 404 - 412, XP025063053 *
ROJAS-CHAPANA J. ET AL.: "Multi-walled carbon nanotubes for plasmid delivery into Escherichia coli cells", LAB CHIPS, vol. 5, 2005, pages 536 - 539, XP008112007 *
See also references of EP2169070A4 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8614366B2 (en) 2009-09-06 2013-12-24 Stephen R. Wilson Methods for genetic plant transformation using water-soluble fullerene derivatives

Also Published As

Publication number Publication date
AU2008264598B2 (en) 2014-06-19
EP2169070A1 (en) 2010-03-31
JP4469959B2 (ja) 2010-06-02
CN101688218B (zh) 2012-10-31
US8420891B2 (en) 2013-04-16
JPWO2008156021A1 (ja) 2010-08-26
AU2008264598A1 (en) 2008-12-24
EP2169070A4 (en) 2010-08-04
CN101688218A (zh) 2010-03-31
US20100248373A1 (en) 2010-09-30
EP2169070B1 (en) 2015-06-10

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