WO2009044846A1 - 薬物誘発性リン脂質症の予測方法 - Google Patents

薬物誘発性リン脂質症の予測方法 Download PDF

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Publication number
WO2009044846A1
WO2009044846A1 PCT/JP2008/068034 JP2008068034W WO2009044846A1 WO 2009044846 A1 WO2009044846 A1 WO 2009044846A1 JP 2008068034 W JP2008068034 W JP 2008068034W WO 2009044846 A1 WO2009044846 A1 WO 2009044846A1
Authority
WO
WIPO (PCT)
Prior art keywords
cells
induced phospholipidosis
amount
predicting drug
drug
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/068034
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English (en)
French (fr)
Inventor
Yoshitaka Tanaka
Kazuhiko Ikeda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyushu University NUC
Astellas Pharma Inc
Original Assignee
Kyushu University NUC
Astellas Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyushu University NUC, Astellas Pharma Inc filed Critical Kyushu University NUC
Priority to US12/674,771 priority Critical patent/US20110117588A1/en
Priority to JP2009536096A priority patent/JP5062578B2/ja
Priority to EP08835363A priority patent/EP2194141A4/en
Priority to CA2697988A priority patent/CA2697988A1/en
Publication of WO2009044846A1 publication Critical patent/WO2009044846A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/918—Carboxylic ester hydrolases (3.1.1)
    • G01N2333/92—Triglyceride splitting, e.g. by means of lipase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

　本発明は、薬物誘発性リン脂質症の予測方法であって、哺乳動物細胞と被検化合物とを接触させる工程、該細胞外および／または細胞内のリソソーム酵素の量もしくは活性を測定するあるいは細胞内のＬＣ３の量を測定する工程、および該酵素の細胞外への分泌を促進したあるいは該蛋白質の量を増加させた被検化合物を、薬物誘発性リン脂質症を誘発し得る化合物として選択する工程を含む方法を提供する。
PCT/JP2008/068034 2007-10-04 2008-10-03 薬物誘発性リン脂質症の予測方法 Ceased WO2009044846A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/674,771 US20110117588A1 (en) 2007-10-04 2008-10-03 Method of predicting drug-induced phospholipidosis
JP2009536096A JP5062578B2 (ja) 2007-10-04 2008-10-03 薬物誘発性リン脂質症の予測方法
EP08835363A EP2194141A4 (en) 2007-10-04 2008-10-03 METHOD FOR PREDICTING MEDICINE-INDUCED PHOSPHOLIPIDOSIS
CA2697988A CA2697988A1 (en) 2007-10-04 2008-10-03 Method of predicting drug-induced phospholipidosis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-261125 2007-10-04
JP2007261125 2007-10-04

Publications (1)

Publication Number Publication Date
WO2009044846A1 true WO2009044846A1 (ja) 2009-04-09

Family

ID=40526268

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/068034 Ceased WO2009044846A1 (ja) 2007-10-04 2008-10-03 薬物誘発性リン脂質症の予測方法

Country Status (5)

Country Link
US (1) US20110117588A1 (ja)
EP (1) EP2194141A4 (ja)
JP (1) JP5062578B2 (ja)
CA (1) CA2697988A1 (ja)
WO (1) WO2009044846A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013114233A (ja) * 2011-11-30 2013-06-10 Olympus Corp 画像処理装置、顕微鏡システム、画像処理方法、及び画像処理プログラム

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9841426B2 (en) * 2009-03-11 2017-12-12 Marker Gene Technologies, Inc Intracellular organelle peptide targeted enzyme substrates
US20200147070A1 (en) * 2017-05-17 2020-05-14 Nextcea Inc. Inducing phospholipidosis for enhancing therapeutic efficacy
CN108929373A (zh) * 2017-05-22 2018-12-04 苏州偶领生物医药有限公司 一种共价键修饰哺乳动物atg8 同源物的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006112947A (ja) 2004-10-15 2006-04-27 Sumitomo Chemical Co Ltd 物質が有するホスホリピドーシス誘発能力の検定方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2434445A (en) * 2006-01-13 2007-07-25 Cytokinetics Inc Assay for phospholipidosis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006112947A (ja) 2004-10-15 2006-04-27 Sumitomo Chemical Co Ltd 物質が有するホスホリピドーシス誘発能力の検定方法

Non-Patent Citations (24)

* Cited by examiner, † Cited by third party
Title
"Dai 35 Kai Annual Meeting of the Japanese Society of Toxicology", 25 June 2008, article KAZUHIKO IKEDA ET AL.: "Shinki in vitro Phospholipidosis Screening-kei no Kochiku" *
"Meth. Enzymol.", vol. 70, article "Immunochemical Techniques"
ANDERSON N. ET AL: "Drug-induced phospholipidosis.", FEBS LETT., vol. 580, September 2006 (2006-09-01), pages 5533 - 5540, XP025232693 *
ATIENZAR F. ET AL: "Determination of phospholipidosis potential based on gene expression analysis in HepG2 cells.", TOXICOL. SCI., vol. 96, no. 1, March 2007 (2007-03-01), pages 101 - 114, XP003026555 *
BARITUSSIO A. ET AL: "Amiodarone inhibits lung degradation of SP-A and perturbs the distribution of lysosomal enzymes.", AM. J. PHYSIOL. LUNG CELL. MOL. PHYSIOL., vol. 281, November 2001 (2001-11-01), pages 1189 - 1199, XP003026552 *
BIOCHEM. PHARMACOL., vol. 53, 1997, pages 1521 - 32
BIOCHEM. PHARMACOL., vol. 62, 2001, pages 1661 - 73
BIOCHIM. BIOPHYS. ACTA, vol. 1631, 2003, pages 136 - 46
CELL BIOL. TOXICOL., vol. 19, 2003, pages 161 - 76
FUJIMURA H. ET AL: "Cell-based fluorescence assay for evaluation of new-drugs potential for phospholipidosis in an early stage of drug development.", EXP. TOXICOL. PATHOL., vol. 58, August 2007 (2007-08-01), pages 375 - 382, XP022155499 *
J. BIOCHEM, vol. 125, 1999, pages 1137 - 1143
KASAHARA T. ET AL: "Establishment of an in vitro high-throughput screening assay for detecting phospholipidosis-inducing potential.", TOXICOL. SCI., vol. 90, no. 1, December 2005 (2005-12-01), pages 133 - 141, XP003026554 *
METHODS IN ENZYMOLOGY, vol. 80, 1981, pages 535 - 561
NIOI P. ET AL: "vitro detection of drug- induced phospholipidosis using gene expression and fluorescent phospholipid based methodologies.", TOXICOL. SCI., vol. 99, no. 1, September 2007 (2007-09-01), pages 162 - 173, XP003026556 *
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See also references of EP2194141A4 *
SEWELL R. B. ET AL: "Perhexilene: effects on hepatic lysosomal function in rats.", CLIN. EXP. PHARMACOL. PHYSIOL., vol. 16, January 1989 (1989-01-01), pages 25 - 32, XP003026553 *
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013114233A (ja) * 2011-11-30 2013-06-10 Olympus Corp 画像処理装置、顕微鏡システム、画像処理方法、及び画像処理プログラム
US9632300B2 (en) 2011-11-30 2017-04-25 Olympus Corporation Image processing apparatus, microscope system, image processing method, and computer-readable recording medium

Also Published As

Publication number Publication date
JP5062578B2 (ja) 2012-10-31
CA2697988A1 (en) 2009-04-09
JPWO2009044846A1 (ja) 2011-02-10
EP2194141A1 (en) 2010-06-09
EP2194141A4 (en) 2010-10-20
US20110117588A1 (en) 2011-05-19

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