WO2009069711A1 - small RNAの取得用担体、取得方法及び取得用試薬 - Google Patents

small RNAの取得用担体、取得方法及び取得用試薬 Download PDF

Info

Publication number
WO2009069711A1
WO2009069711A1 PCT/JP2008/071576 JP2008071576W WO2009069711A1 WO 2009069711 A1 WO2009069711 A1 WO 2009069711A1 JP 2008071576 W JP2008071576 W JP 2008071576W WO 2009069711 A1 WO2009069711 A1 WO 2009069711A1
Authority
WO
WIPO (PCT)
Prior art keywords
small rna
carrier
reagent
obtaining
particle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/071576
Other languages
English (en)
French (fr)
Inventor
Takahiro Nishibu
Sohei Funaoka
Yuzo Hamaguchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sumitomo Bakelite Co Ltd
Fujifilm Wako Pure Chemical Corp
Original Assignee
Wako Pure Chemical Industries Ltd
Sumitomo Bakelite Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries Ltd, Sumitomo Bakelite Co Ltd filed Critical Wako Pure Chemical Industries Ltd
Priority to US12/744,797 priority Critical patent/US20100311958A1/en
Priority to EP08855276.5A priority patent/EP2216406B1/en
Publication of WO2009069711A1 publication Critical patent/WO2009069711A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C12N2310/141—MicroRNAs, miRNAs

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

　本発明は、（１）核となる粒子の表面に重合性官能基又は連鎖移動基を導入し、該粒子と重合性モノマーを混合し、次いで重合反応を進行させることにより、該粒子表面に高分子化合物を含む層を形成した粒子とし、更にその表面上に、small RNA結合タンパク質に対して親和性を有する生理活性物質を固定化した、small RNA取得用担体、（２）上記担体を用いることを特徴とするsmall RNAの取得方法、（３）上記担体を含んでなるsmall RNA又は／及びsmall RNA標的核酸の取得用試薬、（４）該試薬を含んでなる、small RNA又は／及びsmall RNA標的核酸の取得用試薬キット、及び（５）上記担体を用いることを特徴とするsmall RNA標的核酸の取得方法に関する。
PCT/JP2008/071576 2007-11-30 2008-11-27 small RNAの取得用担体、取得方法及び取得用試薬 Ceased WO2009069711A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/744,797 US20100311958A1 (en) 2007-11-30 2008-11-27 Carrier, method and reagent for obtaining small rna
EP08855276.5A EP2216406B1 (en) 2007-11-30 2008-11-27 Carrier, method and reagent for obtaining small rna

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007-311317 2007-11-30
JP2007311317 2007-11-30
JP2008129068A JP5349838B2 (ja) 2007-11-30 2008-05-16 ｓｍａｌｌＲＮＡの取得用担体、取得方法及び取得用試薬
JP2008-129068 2008-05-16

Publications (1)

Publication Number Publication Date
WO2009069711A1 true WO2009069711A1 (ja) 2009-06-04

Family

ID=40678603

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/071576 Ceased WO2009069711A1 (ja) 2007-11-30 2008-11-27 small RNAの取得用担体、取得方法及び取得用試薬

Country Status (4)

Country Link
US (1) US20100311958A1 (ja)
EP (1) EP2216406B1 (ja)
JP (1) JP5349838B2 (ja)
WO (1) WO2009069711A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130197206A1 (en) * 2010-09-24 2013-08-01 Wako Pure Chemical Industries, Ltd. Method for acquisition of small rna

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100062475A1 (en) * 2007-01-16 2010-03-11 Sumitomo Bakelite Company Particle for medical use, particle for anlaysis and method of producing the same
EP2721179A4 (en) * 2011-06-16 2014-10-01 Caris Life Sciences Luxembourg Holdings S A R L BIOMARKER COMPOSITIONS AND METHOD THEREFOR
JP2013019713A (ja) * 2011-07-08 2013-01-31 Sumitomo Bakelite Co Ltd 遺伝子物質固定化医療用粒子および遺伝子物質の捕捉方法
CA2878165A1 (en) * 2012-07-06 2014-01-09 Vertellus Specialties Inc. Olefin-maleic anhydride copolymer compositions and uses thereof
CA2902885A1 (en) 2013-03-15 2014-09-25 Vertellus Specialties Inc. Impact-modified polyamide compositions
BR112016011770B1 (pt) * 2013-11-27 2022-10-25 Sigma-Aldrich Co. Llc Método e kit para isolamento de microrna a partir de amostra biológica
TWI805739B (zh) * 2018-04-17 2023-06-21 臺北榮民總醫院 阻斷緊迫導致腫瘤生成之方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0541946B2 (ja) 1983-08-05 1993-06-25 Wako Pure Chem Ind Ltd
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
WO2005029095A1 (ja) * 2003-09-19 2005-03-31 Sumitomo Bakelite Co., Ltd. バイオチップ
WO2007037069A1 (ja) * 2005-09-27 2007-04-05 Sumitomo Bakelite Company, Ltd. 医療用粒子及びその製造方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7459146B2 (en) * 2003-05-30 2008-12-02 3M Innovative Properties Company Stabilized aerosol dispersions
JP4347211B2 (ja) * 2004-12-24 2009-10-21 住友ベークライト株式会社 バイオチップ用基板およびバイオチップ
US7264872B2 (en) * 2004-12-30 2007-09-04 3M Innovative Properties Company Durable high index nanocomposites for AR coatings
JP2006258458A (ja) * 2005-03-15 2006-09-28 Sumitomo Bakelite Co Ltd 医療材料用高分子化合物及び該高分子化合物を用いたバイオチップ用基板
AU2006248394B2 (en) * 2005-05-19 2012-11-01 Sumitomo Bakelite Company, Ltd. High molecular compound for medical material and biochip substrate using such high molecular compound
US20100062475A1 (en) * 2007-01-16 2010-03-11 Sumitomo Bakelite Company Particle for medical use, particle for anlaysis and method of producing the same
JP5365623B2 (ja) * 2008-03-11 2013-12-11 住友ベークライト株式会社 生理活性物質の固定化方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0541946B2 (ja) 1983-08-05 1993-06-25 Wako Pure Chem Ind Ltd
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
WO2005029095A1 (ja) * 2003-09-19 2005-03-31 Sumitomo Bakelite Co., Ltd. バイオチップ
WO2007037069A1 (ja) * 2005-09-27 2007-04-05 Sumitomo Bakelite Company, Ltd. 医療用粒子及びその製造方法

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
EUR. J. IMMUNOL., vol. 6, 1976, pages 511
IKEDA ET AL., JOURNAL OF IMMUNOLOGICAL METHODS, 2006, pages 10417
IKEDA KEIGO ET AL.: "Detection of the argonaute protein Ago2 and microRNAs in the RNA induced silencing complex (RISC) using a monoclonal antibody.", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 317, 2006, pages 38 - 44, XP025158240 *
JOACIM, NUCLEIC ACID RESEARCH, vol. 36, no. 4, 2008
JOHNSON, CELL, vol. 120, 2005, pages 635 - 647
KOHLER; MILSTEIN, NATURE, vol. 256, 1975, pages 495
MIKIKO SHIOMI: "Hito Argonaute2 (hAgo2) ni Taisuru Monoclonal Kotai no Sakusei", WAKO JUN'YAKU JIHO, vol. 74, no. 4, 2006, pages 2 - 4 *
See also references of EP2216406A4
SUVENDRA, CELL, vol. 125, 2006, pages 1111 - 1124

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130197206A1 (en) * 2010-09-24 2013-08-01 Wako Pure Chemical Industries, Ltd. Method for acquisition of small rna

Also Published As

Publication number Publication date
EP2216406B1 (en) 2014-01-15
US20100311958A1 (en) 2010-12-09
JP5349838B2 (ja) 2013-11-20
JP2009148243A (ja) 2009-07-09
EP2216406A4 (en) 2012-01-04
EP2216406A1 (en) 2010-08-11

Similar Documents

Publication Publication Date Title
JP2024001119A (ja) オリゴヌクレオチドにコードされた化学ライブラリ
WO2003089906A3 (en) Functionalized nanoparticles and methods of use
WO2009135093A3 (en) Rnase-h-based assays utilizing modified rna monomers
Huang et al. Catalytic-hairpin-assembly-assisted DNA tetrahedron nanoprobe for intracellular microRNA imaging
CN116194592A (zh) 流动池系统和装置
AU2022417519A1 (en) Flow cells and methods
WO2001092572A1 (en) Kit and method for determining hla type
WO2007044727A3 (en) Pkr activation via hybridization chain reaction
WO2006083800A3 (en) Nucleic acid silencing of huntington's disease gene
WO2005030929A3 (en) Polymeric nucleic acid hybridization probes
CN111286526A (zh) 一种荧光检测信号放大体系
ATE521620T1 (de) Behandlung von alzheimer-krankheit durch rna- interferenz unter verwendung von short interfering nucleic acid (sina)
Peng et al. Magnetic nanobeads and de novo growth of electroactive polymers for ultrasensitive microRNA detection at the cellular level
WO2006042001A3 (en) Stable three enzyme creatinine biosensor
US12416041B2 (en) Kits and flow cells
US20250361557A1 (en) Immobilization in flow cells
WO2008016562A3 (en) Nucleic acid amplification using a reversibly modified oligonucleotide
WO2005054270A3 (en) Identification and design of sirna molecules
WO2006042022A3 (en) Array-based methods for producing ribonucleic acids
WO2009063998A1 (ja) 疎水性物質付加核酸及びその使用
Hu et al. A multifunctional monolithic interfacial sensor based on gold nanoparticle
CN103451265A (zh) 电场作用下用微孔阵列固液相捕获单分子模板dna的方法
DE602004004710D1 (de) Poröses Harzkorn und Verfahren für dessen Herstellung
Singh et al. Development of paper-based assay for detection of miRNA in various diseases
WO2001076633A3 (de) Gezielte transfektion von zellen mittels biotinyliertem dendrimer

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08855276

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2008855276

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 12744797

Country of ref document: US