WO2009126877A3 - Procédés et compositions pour accélérer la génération de cellules t régulatrices ex vivo - Google Patents

Procédés et compositions pour accélérer la génération de cellules t régulatrices ex vivo Download PDF

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Publication number
WO2009126877A3
WO2009126877A3 PCT/US2009/040190 US2009040190W WO2009126877A3 WO 2009126877 A3 WO2009126877 A3 WO 2009126877A3 US 2009040190 W US2009040190 W US 2009040190W WO 2009126877 A3 WO2009126877 A3 WO 2009126877A3
Authority
WO
WIPO (PCT)
Prior art keywords
regulatory
cells
agents
compositions
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/040190
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English (en)
Other versions
WO2009126877A2 (fr
Inventor
David A. Horwitz
J. Dixon Gray
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Southern California USC
Original Assignee
University of Southern California USC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Southern California USC filed Critical University of Southern California USC
Priority to JP2011504202A priority Critical patent/JP2011519271A/ja
Priority to CN2009801230830A priority patent/CN102066557A/zh
Priority to AU2009234210A priority patent/AU2009234210A1/en
Priority to EP09729610A priority patent/EP2281031A2/fr
Priority to CA2721088A priority patent/CA2721088A1/fr
Publication of WO2009126877A2 publication Critical patent/WO2009126877A2/fr
Publication of WO2009126877A3 publication Critical patent/WO2009126877A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/20—Cellular immunotherapy characterised by the effect or the function of the cells
    • A61K40/22—Immunosuppressive or immunotolerising
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/416—Antigens related to auto-immune diseases; Preparations to induce self-tolerance
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/31—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterized by the route of administration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/38—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterised by the dose, timing or administration schedule
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Transplantation (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La présente invention concerne la génération de cellules T régulatrices par traitement d'une culture cellulaire qui comprend des cellules T non-régulatrices avec une composition régulatrice. L'invention concerne des procédés d'utilisation d'une composition régulatrice qui comprend des agents qui empêchent une méthylation du lieu pour le facteur de transcription FOXP3, des agents qui accélèrent une différenciation des cellules T dans des cellules suppresseurs, et des agents qui sont des inhibiteurs d’histone déacétylase. L'invention concerne également des compositions de cellules T régulatrices générées par culture de cellules T non-régulatrices avec une composition régulatrice, de même que l'utilisation de telles cellules T régulatrices dans le traitement de maladies auto-immunes et de réponses immunitaires aberrantes.
PCT/US2009/040190 2008-04-11 2009-04-10 Procédés et compositions pour accélérer la génération de cellules t régulatrices ex vivo Ceased WO2009126877A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2011504202A JP2011519271A (ja) 2008-04-11 2009-04-10 エクスビボで制御性ｔ細胞の作成を加速するための方法および組成物
CN2009801230830A CN102066557A (zh) 2008-04-11 2009-04-10 加速离体产生调节性t细胞的方法和组合物
AU2009234210A AU2009234210A1 (en) 2008-04-11 2009-04-10 Methods and compositions for accelerating the generation of regulatory Tcells ex vivo
EP09729610A EP2281031A2 (fr) 2008-04-11 2009-04-10 Procédés et compositions pour accélérer la génération de cellules t régulatrices ex vivo
CA2721088A CA2721088A1 (fr) 2008-04-11 2009-04-10 Procedes et compositions pour accelerer la generation de cellules t regulatrices ex vivo

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4430608P 2008-04-11 2008-04-11
US61/044,306 2008-04-11

Publications (2)

Publication Number Publication Date
WO2009126877A2 WO2009126877A2 (fr) 2009-10-15
WO2009126877A3 true WO2009126877A3 (fr) 2009-12-03

Family

ID=40853828

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/040190 Ceased WO2009126877A2 (fr) 2008-04-11 2009-04-10 Procédés et compositions pour accélérer la génération de cellules t régulatrices ex vivo

Country Status (7)

Country Link
US (1) US20090257988A1 (fr)
EP (1) EP2281031A2 (fr)
JP (1) JP2011519271A (fr)
CN (1) CN102066557A (fr)
AU (1) AU2009234210A1 (fr)
CA (1) CA2721088A1 (fr)
WO (1) WO2009126877A2 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9228172B2 (en) * 2008-06-19 2016-01-05 The Trustees Of The University Of Pennsylvania Inducible regulatory T-cell generation for hematopoietic transplants
WO2010135255A1 (fr) * 2009-05-18 2010-11-25 Therakos, Inc. Procédé pour l'expansion ex vivo de lymphocytes t régulateurs avec une fonction suppressive augmentée pour application clinique dans des maladies à médiation immunitaire
CN108467852A (zh) * 2010-04-22 2018-08-31 南加州大学 用于扩增和稳定天然调节性t细胞的方法和组合物
CN102321580A (zh) * 2011-08-23 2012-01-18 郑颂国 一种治疗自体自身免疫性疾病的调节t细胞及其制备方法
JP2016516046A (ja) 2013-03-14 2016-06-02 ジェネンテック， インコーポレイテッド がんの治療方法及びがん薬物耐性を阻止する方法
EA201691443A1 (ru) * 2014-01-17 2016-12-30 Дбв Текнолоджиз Накожное восстановление иммунобаланса
WO2016074580A1 (fr) * 2014-11-14 2016-05-19 中国科学院上海生命科学研究院 Réactif pour l'amélioration du taux de survie de lymphocytes t cd4 positifs et son application
US10905706B2 (en) 2015-01-08 2021-02-02 The Johns Hopkins University Compositions and methods to accelerate resolution of acute lung inflammation
US11207393B2 (en) * 2015-10-16 2021-12-28 President And Fellows Of Harvard College Regulatory T cell PD-1 modulation for regulating T cell effector immune responses
IL258935B2 (en) * 2015-10-28 2024-08-01 Life Tech As Selective expansion of different subpopulations of t cells by the alteration of cell surfacing signals and signal ratio
WO2018106885A1 (fr) 2016-12-07 2018-06-14 East Carolina University Compositions et méthodes de culture et/ou de croissance in vitro de lymphocytes t régulateurs
CN114921468A (zh) 2017-01-30 2022-08-19 国立大学法人京都大学 新型化合物以及调节性t细胞的制造方法
CN108060129A (zh) * 2017-12-11 2018-05-22 上海药明生物技术有限公司 调节性t细胞体外扩增方法
WO2019125981A1 (fr) * 2017-12-19 2019-06-27 Musc Foundation Of Research Development Translantation de lymphocytes t régulateurs (trey) dans l'ostéogenèse imparfaite (oi)
US20210238551A1 (en) * 2018-08-22 2021-08-05 Osaka University Method for Generating Regulatory T Cells
WO2020069377A1 (fr) * 2018-09-27 2020-04-02 Arizona Board Of Regents On Behalf Of The University Of Arizona Procédés d'utilisation de l'inhibition de la lysine désacétylase (kdac) pour générer des réponses de lymphocytes t à mémoire spécifique à un antigène pour une immunothérapie durable
KR20230019157A (ko) * 2020-06-01 2023-02-07 더 헨리 엠. 잭슨 파운데이션 포 더 어드벤스먼트 오브 밀리터리 메디신, 인코포레이티드 유도 조절 t 세포, 이의 제조 방법, 및 용도
GB202008957D0 (en) * 2020-06-12 2020-07-29 Autolus Ltd Culture medium
CN113502265B (zh) * 2021-05-25 2024-05-28 昭泰英基生物医药（香港）有限公司 将t细胞重编程为类nk细胞的诱导剂及其应用
CN119592507A (zh) * 2024-12-03 2025-03-11 哈尔滨优莱福生物科技有限公司 一种人外周血调节性t细胞的体外扩增试剂盒及其使用方法和应用

Citations (1)

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EP1826279A1 (fr) * 2006-02-28 2007-08-29 Charite-Universitätsmedizin Berlin Détection et contrôle de la qualité de lymphocytes T de régulation via l'analyse de méthylation de l'ADN du gène FoxP3

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US20030157057A1 (en) * 1999-05-05 2003-08-21 Horwitz David A. Methods for the induction of professional and cytokine-producing regulatory T cells
US20030147865A1 (en) * 2002-02-07 2003-08-07 Benoit Salomon Cell therapy using immunoregulatory T-cells
WO2007084775A2 (fr) * 2006-01-20 2007-07-26 The Trustees Of The University Of Pennsylvania Compositions et procedes permettant de moduler l’activation d'un lymphocyte t suppresseur
EP2167647A2 (fr) * 2007-06-13 2010-03-31 La Jolla Institute For Allergy And Immunology Lymphocytes t régulateurs et procédé pour les préparer et les utiliser

Patent Citations (1)

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EP1826279A1 (fr) * 2006-02-28 2007-08-29 Charite-Universitätsmedizin Berlin Détection et contrôle de la qualité de lymphocytes T de régulation via l'analyse de méthylation de l'ADN du gène FoxP3

Non-Patent Citations (8)

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Title
BARON UDO ET AL: "DNA demethylation in the human FOXP3 locus discriminates regulatory T cells from activated FOXP3(+) conventional T cells", EUROPEAN JOURNAL OF IMMUNOLOGY, WILEY - V C H VERLAG GMBH & CO. KGAA, DE, vol. 37, no. 9, 1 September 2007 (2007-09-01), pages 2378 - 2389, XP002503237, ISSN: 0014-2980 *
FLOESS STEFAN ET AL: "EPIGENETIC CONTROL OF THE FOXP3 LOCUS IN REGULATORY T CELLS", PLOS BIOLOGY, PUBLIC LIBRARY OF SCIENCE, US, vol. 5, no. 2, 1 February 2007 (2007-02-01), pages 169 - 178, XP008079507, ISSN: 1544-9173 *
HORWITZ DAVID A ET AL: "Critical role of IL-2 and TGF-beta in generation, function and stabilization of Foxp3+CD4+ Treg.", EUROPEAN JOURNAL OF IMMUNOLOGY APR 2008, vol. 38, no. 4, April 2008 (2008-04-01), pages 912 - 915, XP002538142, ISSN: 0014-2980 *
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ZORN EMMANUEL ET AL: "IL-2 REGULATES FOXP3 EXPRESSION IN HUMAN CD4(+)CD25(+) REGULATORY T CELLS THROUGH A STAT-DEPENDENT MECHANISM AND INDUCES THE EXPANSION OF THESE CELLS IN VIVO", BLOOD, AMERICAN SOCIETY OF HEMATOLOGY, US, vol. 108, no. 5, 1 September 2006 (2006-09-01), pages 1571 - 1579, XP008079309, ISSN: 0006-4971 *

Also Published As

Publication number Publication date
EP2281031A2 (fr) 2011-02-09
US20090257988A1 (en) 2009-10-15
CA2721088A1 (fr) 2009-10-15
AU2009234210A2 (en) 2010-12-16
AU2009234210A1 (en) 2009-10-15
CN102066557A (zh) 2011-05-18
WO2009126877A2 (fr) 2009-10-15
JP2011519271A (ja) 2011-07-07

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