WO2009133473A3 - Isothermal detection methods and uses thereof - Google Patents

Isothermal detection methods and uses thereof Download PDF

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Publication number
WO2009133473A3
WO2009133473A3 PCT/IB2009/006280 IB2009006280W WO2009133473A3 WO 2009133473 A3 WO2009133473 A3 WO 2009133473A3 IB 2009006280 W IB2009006280 W IB 2009006280W WO 2009133473 A3 WO2009133473 A3 WO 2009133473A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection methods
methods
detection
isothermal
isothermal detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2009/006280
Other languages
French (fr)
Other versions
WO2009133473A2 (en
Inventor
David James Saul
Leo Samuel Payne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ZyGEM Corp Ltd
Original Assignee
ZyGEM Corp Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ZyGEM Corp Ltd filed Critical ZyGEM Corp Ltd
Priority to JP2010541864A priority Critical patent/JP2011509091A/en
Priority to AU2009241289A priority patent/AU2009241289A1/en
Priority to US12/812,202 priority patent/US20110020819A1/en
Priority to EP09738486A priority patent/EP2242858A4/en
Priority to CN2009801020902A priority patent/CN101970677A/en
Publication of WO2009133473A2 publication Critical patent/WO2009133473A2/en
Anticipated expiration legal-status Critical
Publication of WO2009133473A3 publication Critical patent/WO2009133473A3/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure relates to methods and probes for rapid, single temperature (isothermal) detection of specific nucleic acid sequences. The methods and probes provide a simple method for detecting bioagents including bacteria and viruses, and the detection of specific genetic markers on any nucleic sequence.
PCT/IB2009/006280 2008-01-08 2009-01-08 Isothermal detection methods and uses thereof Ceased WO2009133473A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2010541864A JP2011509091A (en) 2008-01-08 2009-01-08 Isothermal detection method and use thereof
AU2009241289A AU2009241289A1 (en) 2008-01-08 2009-01-08 Isothermal detection methods and uses thereof
US12/812,202 US20110020819A1 (en) 2008-01-08 2009-01-08 Isothermal detection methods and uses thereof
EP09738486A EP2242858A4 (en) 2008-01-08 2009-01-08 Isothermal detection methods and uses thereof
CN2009801020902A CN101970677A (en) 2008-01-08 2009-01-08 Isothermal detection methods and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1980908P 2008-01-08 2008-01-08
US61/019,809 2008-01-08

Publications (2)

Publication Number Publication Date
WO2009133473A2 WO2009133473A2 (en) 2009-11-05
WO2009133473A3 true WO2009133473A3 (en) 2010-07-22

Family

ID=41255489

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2009/006280 Ceased WO2009133473A2 (en) 2008-01-08 2009-01-08 Isothermal detection methods and uses thereof

Country Status (7)

Country Link
US (1) US20110020819A1 (en)
EP (1) EP2242858A4 (en)
JP (1) JP2011509091A (en)
KR (1) KR20100126676A (en)
CN (1) CN101970677A (en)
AU (1) AU2009241289A1 (en)
WO (1) WO2009133473A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ548731A (en) * 2006-07-24 2008-12-24 Zygem Corp Ltd Isothermal detection methods and uses thereof
ES2735514T3 (en) 2009-02-03 2019-12-19 Ande Corp Nucleic acid purification
CN111979303A (en) * 2020-08-11 2020-11-24 上海奕谱生物科技有限公司 Nucleic acid detection kit, method and application thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040096825A1 (en) * 1999-04-30 2004-05-20 Aclara Biosciences, Inc. Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
US20050026193A1 (en) * 1998-07-02 2005-02-03 Kris Richard M. High throughput assay system
US20060292592A1 (en) * 2005-02-09 2006-12-28 Stratagene California Key probe compositions and methods for polynucleotide detection
US20070065816A1 (en) * 2002-05-17 2007-03-22 Affymetrix, Inc. Methods for genotyping
US20070238096A1 (en) * 2006-03-09 2007-10-11 The Regents Of The University Of California Hybrid energy transfer for nucleic acid detection
US20070292861A1 (en) * 2004-05-20 2007-12-20 Trillion Genomics Limited Use of Mass Labelled Probes to Detect Target Nucleic Acids Using Mass Spectrometry
WO2008013462A2 (en) * 2006-07-24 2008-01-31 Zygem Corporation Limited Isothermal detection methods and uses thereof

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5011769A (en) * 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5403711A (en) * 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5413924A (en) * 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
WO1995005480A2 (en) * 1993-08-18 1995-02-23 Id Biomedical Corporation Compositions and methods for detecting target nucleic acid sequences utilizing flanking sequence enzyme molecules
AU8102694A (en) * 1993-11-17 1995-06-06 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
DE4441626A1 (en) * 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Method for the particularly sensitive detection of nucleic acids
US6001610A (en) * 1994-11-23 1999-12-14 Roche Diagnostics, Gmbh Method for the particularly sensitive detection of nucleic acids
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
US5656430A (en) * 1995-06-07 1997-08-12 Trevigen, Inc. Oscillating signal amplifier for nucleic acid detection
US6153425A (en) * 1995-07-13 2000-11-28 Xtrana, Inc. Self-contained device integrating nucleic acid extraction, amplification and detection
US5747255A (en) * 1995-09-29 1998-05-05 Lynx Therapeutics, Inc. Polynucleotide detection by isothermal amplification using cleavable oligonucleotides
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
WO1999055914A1 (en) * 1998-04-29 1999-11-04 Trustees Of Boston University Methods and compositions pertaining to pd-loops
US6326173B1 (en) * 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
US6893819B1 (en) * 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US6803196B1 (en) * 2000-10-13 2004-10-12 Affymetrix, Inc. Methods and compositions for detecting signals in binding assays using microparticles
NZ511680A (en) * 2001-05-14 2004-07-30 Univ Waikato Method for preparing nucleic acid or DNA samples and a DNA extraction process using thermophilic proteases
CA2449911A1 (en) * 2001-06-15 2002-12-27 Quiatech Ab Amplifiable probe
CN102203284A (en) * 2008-08-14 2011-09-28 赛进有限公司 Temperature controlled nucleic-acid detection method suitable for practice in a closed-system

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050026193A1 (en) * 1998-07-02 2005-02-03 Kris Richard M. High throughput assay system
US20040096825A1 (en) * 1999-04-30 2004-05-20 Aclara Biosciences, Inc. Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
US20070065816A1 (en) * 2002-05-17 2007-03-22 Affymetrix, Inc. Methods for genotyping
US20070292861A1 (en) * 2004-05-20 2007-12-20 Trillion Genomics Limited Use of Mass Labelled Probes to Detect Target Nucleic Acids Using Mass Spectrometry
US20060292592A1 (en) * 2005-02-09 2006-12-28 Stratagene California Key probe compositions and methods for polynucleotide detection
US20070238096A1 (en) * 2006-03-09 2007-10-11 The Regents Of The University Of California Hybrid energy transfer for nucleic acid detection
WO2008013462A2 (en) * 2006-07-24 2008-01-31 Zygem Corporation Limited Isothermal detection methods and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2242858A4 *

Also Published As

Publication number Publication date
US20110020819A1 (en) 2011-01-27
AU2009241289A1 (en) 2009-11-05
EP2242858A4 (en) 2011-01-26
WO2009133473A2 (en) 2009-11-05
CN101970677A (en) 2011-02-09
EP2242858A2 (en) 2010-10-27
KR20100126676A (en) 2010-12-02
JP2011509091A (en) 2011-03-24

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