WO2011069487A3 - Dna-aptamere, die den löslichen interleukin-6-rezeptor spezifisch binden - Google Patents

Dna-aptamere, die den löslichen interleukin-6-rezeptor spezifisch binden Download PDF

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Publication number
WO2011069487A3
WO2011069487A3 PCT/DE2010/001413 DE2010001413W WO2011069487A3 WO 2011069487 A3 WO2011069487 A3 WO 2011069487A3 DE 2010001413 W DE2010001413 W DE 2010001413W WO 2011069487 A3 WO2011069487 A3 WO 2011069487A3
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WO
WIPO (PCT)
Prior art keywords
receptor
soluble interleukin
specifically bind
dna aptamers
aim
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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PCT/DE2010/001413
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English (en)
French (fr)
Other versions
WO2011069487A2 (de
Inventor
Ulrich Hahn
Stefan Rose-John
Cindy Meyer
Tijana Zivkovic
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Christian Albrechts Universitaet Kiel
Universitaet Hamburg
Original Assignee
Christian Albrechts Universitaet Kiel
Universitaet Hamburg
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Application filed by Christian Albrechts Universitaet Kiel, Universitaet Hamburg filed Critical Christian Albrechts Universitaet Kiel
Publication of WO2011069487A2 publication Critical patent/WO2011069487A2/de
Publication of WO2011069487A3 publication Critical patent/WO2011069487A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Zielmolekül spezifisch bindende Aptamere. Aufgabe der Erfindung ist es, diagnostische und/oder therapeutische Mittel zur Anwendung bei der Erkennung und/oder Prävention bzw. Behandlung von Entzündungsprozessen, Krebserkrankungen und Infektionen bereitzustellen. Zur Lösung dieser Aufgabe stellt die Erfindung ein DNA-Aptamer bereit, das den menschlichen löslichen Interleukin-6-Rezeptor (sIL-6R) spezifisch bindet und die Sequenz GGGNGGGHGGGWGGG, ausgenommen jedoch die Sequenz GGGTGGGTGGGTGGGT, umfasst, wobei N = A, T, TT oder TTT ist, H = A, C oder T ist und W = A oder T ist.
PCT/DE2010/001413 2009-12-07 2010-12-06 Dna-aptamere, die den löslichen interleukin-6-rezeptor spezifisch binden Ceased WO2011069487A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102009056945A DE102009056945A1 (de) 2009-12-07 2009-12-07 DNA-Aptamere, die den löslichen Interleukin-6-Rezeptor spezifisch binden
DE102009056945.6 2009-12-07

Publications (2)

Publication Number Publication Date
WO2011069487A2 WO2011069487A2 (de) 2011-06-16
WO2011069487A3 true WO2011069487A3 (de) 2011-10-13

Family

ID=43867191

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2010/001413 Ceased WO2011069487A2 (de) 2009-12-07 2010-12-06 Dna-aptamere, die den löslichen interleukin-6-rezeptor spezifisch binden

Country Status (2)

Country Link
DE (1) DE102009056945A1 (de)
WO (1) WO2011069487A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115851742B (zh) * 2022-10-28 2025-10-10 合肥工业大学 一种IL-6/sIL-6R双特异性环状核酸适配体及试剂盒与应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994025037A1 (en) * 1993-04-23 1994-11-10 Triplex Pharmaceutical Corporation Anti-viral guanosine-rich oligonucleotides

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1493825A3 (de) 1990-06-11 2005-02-09 Gilead Sciences, Inc. Verfahren zur Herstellung von Nukleinsäureliganden
US6423493B1 (en) * 1998-10-26 2002-07-23 Board Of Regents The University Of Texas System Combinatorial selection of oligonucleotide aptamers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994025037A1 (en) * 1993-04-23 1994-11-10 Triplex Pharmaceutical Corporation Anti-viral guanosine-rich oligonucleotides

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DAUSSE E ET AL: "Aptamers: a new class of oligonucleotides in the drug discovery pipeline?", CURRENT OPINION IN PHARMACOLOGY, ELSEVIER SCIENCE PUBLISHERS, NL, vol. 9, no. 5, 1 October 2009 (2009-10-01), pages 602 - 607, XP026665554, ISSN: 1471-4892, [retrieved on 20090828], DOI: DOI:10.1016/J.COPH.2009.07.006 *
GATTO B ET AL: "Nucleic Acid Aptamers Based on the G-Quadruplex Structure: Therapeutic and Diagnostic Potential", CURRENT MEDICINAL CHEMISTRY, vol. 16, no. 10, April 2009 (2009-04-01), pages 1248 - 1265, XP002634830, ISSN: 0929-8673 *
JUNICHI YAMAGUCHI1 ET AL: "cDNA display: a novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA-protein fusions", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 37, no. 16, 1 September 2009 (2009-09-01), pages 1 - 13, XP002631169, ISSN: 0305-1048, [retrieved on 20090615], DOI: DOI:10.1093/NAR/GKP514 *
MAJUMDER PAROMITA ET AL: "Aptamers: from bench side research towards patented molecules with therapeutic applications", EXPERT OPINION ON THERAPEUTIC PATENTS, INFORMA HEALTHCARE, GB, vol. 19, no. 11, 1 November 2009 (2009-11-01), pages 1603 - 1613, XP002628467, ISSN: 1354-3776, DOI: DOI:10.1517/13543770903313746 *
MICHALOWSKI DANIEL ET AL: "Novel bimodular DNA aptamers with guanosine quadruplexes inhibit phylogenetically diverse HIV-1 reverse transcriptases.", NUCLEIC ACIDS RESEARCH DEC 2008 LNKD- PUBMED:18996899, vol. 36, no. 22, December 2008 (2008-12-01), pages 7124 - 7135, XP002634829, ISSN: 1362-4962 *
PHAN ANH TUÂN ET AL: "An interlocked dimeric parallel-stranded DNA quadruplex: a potent inhibitor of HIV-1 integrase.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 18 JAN 2005 LNKD- PUBMED:15637158, vol. 102, no. 3, 18 January 2005 (2005-01-18), pages 634 - 639, XP002634933, ISSN: 0027-8424 *
SATORU SHINRIKI ET AL: "Humanized Anti-Interleukin-6 Receptor Antibody Suppresses Tumor Angiogenesis and In vivo Growth of Human Oral Squamous Cell Carcinoma", CLINICAL CANCER RESEARCH, THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, US, vol. 15, no. 17, 1 September 2009 (2009-09-01), pages 5426 - 5434, XP002628465, ISSN: 1078-0432, [retrieved on 20090825], DOI: DOI:10.1158/1078-0432.CCR-09-0287 *
TIJANA ZIVKOVIC: "Interleukin-6-Rezeptor-spezifische DNA-Aptamere", 12 May 2010 (2010-05-12), XP002634831, Retrieved from the Internet <URL:http://ediss.sub.uni-hamburg.de/volltexte/2010/4602/pdf/Dissertation_Tijana_Zivkovic_2010.pdf> [retrieved on 20110428] *
YANG QING ET AL: "DNA ligands that bind tightly and selectively to cellobiose", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 95, no. 10, 12 May 1998 (1998-05-12), pages 5462 - 5467, XP002634828, ISSN: 0027-8424 *
YOKOTA SHUMPEI ET AL: "Clinical study of tocilizumab in children with systemic-onset juvenile idiopathic arthritis", CLINICAL REVIEWS IN ALLERGY AND IMMUNOLOGY, HUMANA PRESS, TOTOWA, NJ, US, vol. 28, no. 3, 1 June 2005 (2005-06-01), pages 231 - 238, XP002628466, ISSN: 1080-0549 *

Also Published As

Publication number Publication date
WO2011069487A2 (de) 2011-06-16
DE102009056945A1 (de) 2011-06-16

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