WO2012091321A2 - Préparation pour favoriser la pousse des cheveux contenant un liquide de culture de cellules souches d'adipocyte et son procédé de préparation - Google Patents

Préparation pour favoriser la pousse des cheveux contenant un liquide de culture de cellules souches d'adipocyte et son procédé de préparation Download PDF

Info

Publication number
WO2012091321A2
WO2012091321A2 PCT/KR2011/009556 KR2011009556W WO2012091321A2 WO 2012091321 A2 WO2012091321 A2 WO 2012091321A2 KR 2011009556 W KR2011009556 W KR 2011009556W WO 2012091321 A2 WO2012091321 A2 WO 2012091321A2
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
hair growth
human body
fat
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2011/009556
Other languages
English (en)
Korean (ko)
Other versions
WO2012091321A3 (fr
Inventor
김영실
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2012091321A2 publication Critical patent/WO2012091321A2/fr
Publication of WO2012091321A3 publication Critical patent/WO2012091321A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird

Definitions

  • the present invention relates to a hair growth agent and a manufacturing method, and more particularly, to a hair growth agent and a method for producing the same using a culture medium in which the fat stem cells are isolated from the human body.
  • Hair is a general term for hair growing on the human body, and it is variously named according to the area of hair. These hairs vary according to body parts, individuals, and races, and Korean hair has a large number of black browns, and whites have a small number of melanin, which are brown, yellow, and red.
  • Hair does not continue to grow as epithelial cells grow as they make keratin fibers, but each has a lifespan, causing hair growth and hair loss to repeat. These hairs are classified into growing stages, intermediate stages in which growth stops and the bottom of the roots rise, and boils in which the hair falls out of the human body.
  • hair growth In the boil, new hair begins to grow, and this cycle is called hair growth, which is different for each individual or part of the human body.
  • the growth rate In the case of provocation, the growth rate is on average 0.2 to 0.4 millimeters per day, the hair cycle is 2 to 6 years, and some hairs take 25 years.
  • hair loss there are about 100,000 hairs on the human scalp, and the condition that occurs as the number of hairs that are supposed to be normal or the number thereof decreases is called hair loss. Unlike in the past, such hair loss is changed by modern factors such as pollution, stress, seasonal changes, diseases, and chemical procedures.
  • stem cells are undifferentiated cells that are capable of differentiating into various other cells, and are largely divided into embryonic stem cells and adult stem cells.
  • embryonic stem cells are largely divided into embryonic stem cells and adult stem cells.
  • studies on adult stem cells for active treatment or commercialization are being actively conducted. to be.
  • the adult stem cells but there are many types, such as bone, muscle, fat, umbilical cord blood, many of them are being studied for fat stem cells extracted from fat.
  • the non-toxic human collagenase is used for the enzyme separation from the fat of the human body by enzymes used in human body fat; A medium for culturing the adipose stem cells in serum extracted from the human body; A culture solution formed while the adipose stem cells are cultured in the medium; And a receptor containing the culture solution; It is configured to include.
  • the method for producing a hair growth agent using the culture medium of the adipose stem cells the first step of enzymatic separation of fat stem cells from the fat of the human body by enzymes using non-toxic human collagenase; A second step of extracting a culture solution formed while the adipose stem cells separated in the first step are cultured in a medium containing serum extracted from a human body; And a third step of receiving the culture solution in a receptor; It is configured to include.
  • the hair growth preparation uses the culture medium of the adipose stem cells according to the present invention and a method for producing the same, the hair growth preparation has a configuration comprising a culture solution of fat stem cells extracted from the human body and a receptor for receiving the same.
  • the hair growth proliferation effect can be expected by the anti-hair loss action and the hair growth action, and specificity of each individual, for example, immune rejection.
  • limiting by a reaction does not arise can be anticipated.
  • the use of the culture supernatant that does not include cells that generate an immune rejection response can be expected to utilize the components of the stem cell without restriction on the immune rejection response.
  • the effect of activating the parental phase of the resting phase can be expected, it can be expected to effect the room temperature storage of the hair growth agent by lecithinization and lyophilization method.
  • Figure 1 is a schematic diagram showing the configuration of the hair growth agent using a culture medium of adipose stem cells according to an embodiment of the present invention.
  • Figure 2 is a block diagram showing a method for producing a hair growth agent using a culture medium of adipose stem cells according to an embodiment of the present invention.
  • Figure 3 is a diagram showing the additive of the hair growth agent using a culture medium of fat stem cells according to an embodiment of the present invention.
  • Figure 4 is a photograph showing the state of the mice coated with a hair growth agent once a day using the culture medium of fat stem cells according to an embodiment of the present invention.
  • Figure 5 is a photograph showing the state of the mice coated with a hair growth agent twice a day using the culture medium of fat stem cells according to an embodiment of the present invention.
  • Figure 6 is a diagram showing the state of the mouse using a hair growth agent using the culture medium of fat stem cells according to an embodiment of the present invention.
  • Figure 7 is a graph showing the state of the mouse using a hair growth agent using the culture medium of fat stem cells according to an embodiment of the present invention.
  • Figure 8 is a photograph showing the state of the scalp using the hair growth agent using the culture medium of the adipose stem cells according to an embodiment of the present invention.
  • FIG. 1 is a schematic diagram showing the configuration of the hair growth agent using the culture medium of the fat stem cells according to an embodiment of the present invention
  • Figure 2 is a method for producing a hair growth agent using the culture medium of fat stem cells according to an embodiment of the present invention. A block diagram is shown.
  • the hair growth agent using the culture medium of the adipose stem cells according to an embodiment of the present invention to accommodate the culture solution 20 and the culture solution 20 obtained by the culture of fat stem cells extracted from the human body as a whole It comprises a receptor 40.
  • the culture solution 20 is obtained by culturing fat stem cells 11 separated from the fat 10 extracted from the human body in the medium 30, the receptor 40 has a form having a predetermined internal space. While being able to selectively open and close to perform the function of receiving the culture solution (20).
  • the receptor 40 may have any configuration as long as it has a predetermined internal space for accommodating the culture solution 20, but is formed of a material that does not react chemically or physically with the culture solution 20.
  • strength is preferable.
  • the receptor 40 may be configured to be selectively opened or closed on any one surface or a portion thereof, for example, and may be configured to be sealed while the culture solution 20 is accommodated.
  • the upper surface is configured to allow selective opening and closing, the user opens and uses the upper surface, and is configured to be shielded and stored after use, and when configured to be closed, the culture medium 20 using another device such as a syringe or a tube. Will be moved to use.
  • Hair growth using the culture medium of the fat stem cells according to an embodiment of the present invention is prepared through a three-step process as a whole.
  • first step (S10) to extract the fat stem cells 11 from the fat (10) separated from the human body.
  • the fat stem cell 11 extracted from the fat 10 is to proceed to the second step (S20).
  • second step (S20) is a process of culturing the fat stem cells (11).
  • the fat stem cells 11 are extracted from the fat stem cells 11 from the fat (10).
  • the fat stem cells 11 extracted from the fat 10 by the progress of the first step (S10) is a small sized undifferentiated cells distributed between cells of the fat 10 tissue, fat cells, skin cells It is a cell that can differentiate into various cells such as hair cells, and stem cells that can differentiate into hair cells in the scalp.
  • the fat 10 is extracted from the human body and then centrifuged. As such, the centrifugation process removes impurities and obtains pure adipose tissue.
  • the fat 10 is centrifuged at about 1400G to 1600G for about 2 minutes 30 seconds to 3 minutes 30 seconds, and pure fat tissue is obtained through such centrifugation.
  • centrifugation of the adipose tissue at about 1500 G for about 3 minutes is preferable, and in this case, it may vary depending on the state and site of the adipose tissue obtained.
  • the enzyme used for enzymatic digestion is used collagenase, which is harmless to the human body.
  • the collagenase is mixed with physiological saline in a ratio of 1: 1 in a sterilized or sterilized state to be harmless to the human body to undergo enzymatic degradation for a time of 30 minutes to 60 minutes at 37 degrees Celsius.
  • the ratio of the collagenase and saline and the reaction temperature, time, etc. in the enzymatic degradation process is proposed as the most suitable value in the embodiment of the present invention by a number of experimental results, the range can be flexible. This means that the experimental data may change if the amount or environment of adipose tissue is applied differently.
  • the collagenase which is harmless to the human body, should be used to degrade the enzyme.
  • the collagenase which is harmless to the human body is used, so that the enzymatic decomposition proceeds, so that a separate neutralizing agent during the enzymatic degradation, that is, the neutralizer for neutralizing the collagenase in a harmless state to the human body, is suppressed.
  • an enzyme that is harmless to the human body should be used in the course of the enzymatic decomposition reaction, and a representative example thereof is collagenana which is harmless to the human body. I will be.
  • the neutralizing agent when the fat 10 cells are obtained by enzymatically decomposing the fat 10 using an enzyme that is harmless to the human body, that is, collagenase, the neutralizing agent is not used without disturbing the human immune response. It has the advantage of not having to.
  • the method of extracting the fat stem cells 11 from the fat 10 the fat 10 is decomposed by the enzyme mixed with collagenase and physiological saline 1: 1 harmless to the human body again Centrifugation.
  • the adipose tissue enzymatically decomposed by the mixture of collagenase and physiological saline, which is harmless to the human body, is subjected to centrifugation once again.
  • the adipose tissue is centrifuged at about 800 G to 1000 G for about 4 to 6 minutes.
  • the adipose tissue is separated into oil and the fat 10, the adipose stem cells 11, and the like, and the adipose stem cells 11 are formed in a pellet form.
  • tissue of the adipose stem cells 11 formed in the form of pellets is removed using a mesh of 90 to 110 micrometers to remove the solid component, washed with physiological saline, centrifuged for about 3 minutes at 250G to 350G. By proceeding to extract to obtain the fat stem cells (11).
  • a second step S20 is performed.
  • the second step (S20) is a process of culturing the fat stem cells 11, thereby culturing the fat stem cells 11 in a medium 30 containing serum extracted from the human body.
  • a medium is a nutrient that is required for cultivating microorganisms or animals and tissues.It is a nutrient substance including water, which is indispensable for survival and development, except that it is obtained in a gaseous state, and is a carbon source, nitrogen source, inorganic salts, and growth factors And the like).
  • a liquid or solid material used for proliferation, preservation, and transportation of the adipose stem cells 11 is called a medium.
  • a medium Such a medium must be sterilized before use and used in a sterile state.
  • the medium 30 There are various types of the medium 30 according to the purpose of use, and inoculated with the inoculation material containing a small amount of bacteria in the sterile growth medium 30 for cultivation and incubated in a thermostat.
  • the medium 30 can be used for various purposes, such as separation culture, passage culture, enrichment, identification test, etc. of the culture medium, and the most widely used as the material of the medium 30 is peptone, meat extract, yeast Extracts.
  • liquid medium to which no solidifying agent is added
  • solid medium to which agar or gelatin is added.
  • the solid medium can be used in a solid form by heating by adding serum.
  • the adipose stem cells are cultured in the second step 20 using Dulbecco's Modified Eagle's Medium (DMEM) medium.
  • DMEM Dulbecco's Modified Eagle's Medium
  • the medium 30 having the serum extracted from the human body is used.
  • the serum extracted from the human body is the most suitable form for the human body, there is no need to use antibiotics, it is excellent in applicability and can minimize the various situations that can occur in animal serum has the advantage of securing safety .
  • a method of using 10% human extracted serum to DMEM medium will be described as an example.
  • the addition amount of the human extract serum will be described by way of example 10% derived by the most suitable addition amount through a number of experiments, the addition amount is variable.
  • vitamin C it is also possible to add ascorbic acid or other additives to supply vitamin C to provide a more enhanced growth environment.
  • the second step (S20) proceeds while the fat stem cells 11 are cultured in the state in which the acrobic acid is added to the medium 30 containing 10% of the serum extracted from the human body.
  • the fat stem cells 11 are cultured in an incubator having a carbon dioxide environment of 5% at a temperature of 37 degrees Celsius.
  • the culture conditions of the second step (S20) is used to derive the most suitable environment through a number of experiments.
  • the culture conditions can be changed depending on the amount of the adipose stem cells 11 or other environments, and in the same environment as in the embodiment of the present invention, a temperature of 37 degrees Celsius and an environment of a carbon dioxide incubator of 5% This is the most suitable environment.
  • the adipose stem cells 11 that are not attached to the medium 30 are washed to maintain about 85 to 90% of the adipose stem cells 11 and then passaging.
  • the subculture is followed by removing the culture medium 20 in which the adipose stem cells 11 are cultured, dropping the adipose stem cells 11 with 0.25% trypsin-EDTA, and then obtaining a cell suspension and cultivating again twice. Way.
  • the fat stem cells 11 are continuously cultured to obtain a culture solution.
  • the passage of the fat stem cells 11 does not proceed indefinitely and repeatedly, but after the predetermined number of times, the culture does not proceed.
  • the passage of the fat stem cells 11 in which the passage was carried out is cultured in a serum-free medium which is substituted by replacing the medium (30) containing serum extracted from the human body with 10% serum-free medium.
  • the passage culture is a method in which the fat stem cells 11 continue to be cultured in succession by moving to a new environment for proliferation of the fat stem cells 11, and in a limited environment, the proliferation of the fat stem cells 11 is performed. Since it is limited, the proliferation of the adipose stem cells 11 is transferred to a new environment in order to provide a new space.
  • Ham's F-12 nutrient mixture is mixed in a ratio of 1: 0.5 to 2.0 in the serum-free medium in which the fat stem cells 11 are cultured, and the fat is added to the mixed serum-free medium by adding ascorbic acid. Stem cells 11 are cultured.
  • the medium 30 in which the fat stem cells 11 are cultured is collected and centrifuged for about 5 minutes at about 1000G. After centrifugation, the debris of the adipose stem cells 11 is removed by a filter of 0.2 micrometer, and the culture solution 20 in which the adipose stem cells 11 are cultured is extracted.
  • the adipose stem cells 11 when the adipose stem cells 11 are passaged, growth factors and protein active substances secreted by the stem cells are absorbed into the medium 30. Each time the fat stem cell 11 is passaged, the culture medium 30 is collected and centrifuged to obtain a supernatant liquid from which the residue is removed as the culture solution 20.
  • the main components contained in the medium 30, such as various growth factors and protein active substances secreted by stem cells, and various substances secreted only by stem cells, are not contained in stem cells, and the Fatty stem cells 11 contain a number of substances secreted.
  • Growth factors secreted by the adipose stem cells 11 include fibroblast growth factor (FGF: fibroblast growth factor), insulin growth factor (IGF: insulin-like growth factor), transforming growth factor (TGF: transforming growth factor) Vascular endothelial growth factor (VEGF), platelet derived growth factor (PDGF), epidermal growth factor (EGF), nerve growth factor (NGF) ), Such growth factors are contained in the culture solution (20).
  • FGF fibroblast growth factor
  • IGF insulin growth factor
  • TGF transforming growth factor
  • VEGF Vascular endothelial growth factor
  • PDGF platelet derived growth factor
  • EGF epidermal growth factor
  • NGF nerve growth factor
  • the third step (S30) is a step of receiving the culture solution 30 in the receptor 40.
  • the culture solution 20 obtained by the progress of the second step (S20) is accommodated in the receptor 40 in the third step (S30).
  • the culture solution 20 may be configured to be accommodated in the receptor 40 as a stock solution of 100%, and may be mixed with sterile physiological saline to be accommodated in the receptor 40.
  • an additive for modifying the shape of the culture solution 20 may be further added to the inner space of the receptor 40, and an additive for enhancing the function of the culture solution 20 may be further added.
  • an emulsifier may be added to modify the form of the culture solution 20 into a gel, or iodine may be further added to enhance the function of the culture solution 20.
  • the culture solution 20 contained in the inner space of the receptor 40 is accommodated in the form of gel or cream or liquid. That is, the configuration may be accommodated in various forms according to the type of additives added as the third step (S30) proceeds.
  • stearic acid and glycerin may be added to the culture solution 20 or the mixture of the culture solution 20 and physiological saline to be accommodated in the receptor 40 to increase steric acid and moisture retention. Do.
  • an additive such as cyclopentasiloxane, cyclomethicone, hydrogenated polyderate may be used, and pentaerythritol as a thickener.
  • a configuration in which additives such as tetrahexanoate, sodupolyacrylate, and acrylate / C-10-30 alkali acrylate crosspolymer are used may also be used.
  • an additive such as distilled water, lotion, mineral water as a solvent, and as an moisturizing agent additives such as dipropylene glycol, glycerin, methylgloss
  • an additive such as distilled water, lotion, mineral water
  • an moisturizing agent additives such as dipropylene glycol, glycerin, methylgloss
  • Such additives may also be configured to use additives such as hydrogenated polyisobutene, ammonium acryloyl dimethyl taurate, V-Picopolymer as a thickener, and the additive concentration of the additive used as the thickener may be added to the gel or cream. It will be added in lesser or lesser concentrations than the agent.
  • additives such as hydrogenated polyisobutene, ammonium acryloyl dimethyl taurate, V-Picopolymer
  • the culture solution 20 or the mixture of the culture solution 20 and the physiological saline solution may be accommodated in various forms in the inner space of the receptor 40, and the user may use it according to his / her preference. It is possible to have the advantage that the ease of use is improved.
  • the user when the culture solution 20 or the mixture of the culture solution 20 and the physiological saline solution is accommodated in the form of a gel or a cream in the inner space of the receptor 40, the user can be used by applying to the scalp, If it is accommodated in the liquid form in the inner space of the receptor 40, the user can be used by spraying the scalp as a spray, the method of injecting with an injection, it is also possible to use in the form of skin applied to the scalp. .
  • arginine a metabolite precursor of nitrogen oxides to promote hair growth, having an hair loss prevention effect as an essential component of hair growth Cysteine, iodine having the function of promoting hair growth, vitamin A to help the formation of keratin, vitamin D having the hair regeneration effect, vitamin E to smooth the blood circulation of the scalp, white hair while preventing hair loss Vitamin H and the like to have a function to prevent.
  • additives are additives added to have a further improved effect, and at least one additive is added to improve the function. That is, it is also possible to add one additive, it is possible to configure a plurality of additives.
  • the user uses the scalp site where hair loss proceeds to induce hair growth.
  • the hair growth agent is configured and manufactured as in the embodiment of the present invention can be distributed in general, the immune rejection of the human body does not occur has the advantage that the safety is improved.
  • Figure 4 is a photograph showing the state of the rat applied a hair growth agent once a day using the culture medium of the adipose stem cells according to an embodiment of the present invention
  • Figure 5 is a diagram of the adipose stem cells according to an embodiment of the present invention This is a photograph showing the state of the mice coated with the hair growth agent twice a day using the culture solution.
  • Figure 6 is a diagram showing the state of the mouse using a hair growth agent using the culture medium of fat stem cells according to an embodiment of the present invention
  • Figure 7 is a culture medium of fat stem cells according to an embodiment of the present invention It is a graph showing the state of the mice using the hair growth agent.
  • Figure 8 is a photograph showing the state of the human body using the hair growth agent using the culture medium of fat stem cells according to an embodiment of the present invention.
  • the mice used in this experiment were run for 10 weeks using mice that had become stressed natural hair loss.
  • the experimental rat before using the hair growth agent 50 of the present invention is a state in which stress or natural hair loss occurs in a part of the back or head.
  • the time required to exhibit a 10% therapeutic effect is variously distributed from about 7 days to 15 days, and it can be seen that the average time is 10.3 days, and the time required to exhibit a 50% therapeutic effect is It can be seen that an average of 22.2 days is required while having a distribution of approximately 17 to 26 days.
  • the time required for displaying the therapeutic effect of 100% has an average of 42.2 days while having a distribution of approximately 35 days to 47 days.
  • the horizontal axis represents a treatment effect
  • the vertical axis represents a period of use.
  • the graph showing the treatment effect for each day using the hair growth agent 50 shows a form having a larger slope after 50% than before 50%, up to 50%. It can be seen that the transverse axis length has a shape longer than the transverse axis length up to 100%.
  • the graph is characterized by a faster treatment effect as the period of use of the hair growth agent 50 is longer.
  • the picture attached to Figure 8 is a picture taken two weeks after the marking on the area where the hair loss phenomenon occurs by applying the hair growth agent 50. Looking at this, after the hair growth agent 50 is applied to the site of the hair loss phenomenon for two weeks can be confirmed that the hair is thickened as the hair thickness of the area where the hair loss phenomenon is increased, the hair is thickened.
  • the hair growth preparation using adipose stem cells according to the present invention and a method for producing the same, efficient hair management can be performed using a culture medium of adipose stem cells, and the effect of preventing hair loss and improving hair growth can be expected.
  • culturing the adipose stem cells in a medium containing serum extracted from the human body it can be expected to produce a stable, suitable culture medium for the stem cells.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Zoology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cosmetics (AREA)

Abstract

La présente invention concerne une préparation pour favoriser la pousse des cheveux et un procédé pour la préparer et, en particulier, une préparation pour favoriser la pousse des cheveux contenant un liquide de culture de cellules souches d'adipocyte prélevées sur le corps humain et un procédé pour la préparer. La préparation de l'invention comprend : des cellules souches d'adipocyte isolées par voie enzymatique de la graisse du corps humain, l'enzyme utilisée étant une collagénase humaine non toxique ; un milieu pour cultiver l'isolat de cellules souches d'adipocyte dans un sérum extrait du corps humain ; un liquide de culture formé par la culture des cellules souches d'adipocyte dans le milieu ; et un récipient pour recevoir le liquide de culture. L'invention concerne également un procédé favorisant la pousse des cheveux, le procédé comprenant : une première étape consistant à isoler par voie enzymatique des cellules souches d'adipocyte de la graisse du corps humain au moyen d'une enzyme, l'enzyme étant une collagénase humaine non toxique ; une deuxième étape consistant à extraire un liquide de culture formé par la culture des cellules souches d'adipocyte isolées dans la première étape, dans un milieu contenant un sérum extrait du corps humain ; et une troisième étape consistant à recevoir le liquide de culture dans un récipient. La préparation peut être stockée à température ambiante et ne provoque pas de réaction immunitaire. En outre, elle peut empêcher la perte des cheveux et favoriser leur croissance.
PCT/KR2011/009556 2010-12-30 2011-12-12 Préparation pour favoriser la pousse des cheveux contenant un liquide de culture de cellules souches d'adipocyte et son procédé de préparation Ceased WO2012091321A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0139275 2010-12-30
KR1020100139275A KR101061464B1 (ko) 2010-12-30 2010-12-30 지방줄기세포의 배양액을 이용한 모발증식제재 및 이의 제조방법

Publications (2)

Publication Number Publication Date
WO2012091321A2 true WO2012091321A2 (fr) 2012-07-05
WO2012091321A3 WO2012091321A3 (fr) 2012-11-08

Family

ID=44956801

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/009556 Ceased WO2012091321A2 (fr) 2010-12-30 2011-12-12 Préparation pour favoriser la pousse des cheveux contenant un liquide de culture de cellules souches d'adipocyte et son procédé de préparation

Country Status (2)

Country Link
KR (1) KR101061464B1 (fr)
WO (1) WO2012091321A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016006885A1 (fr) * 2014-07-07 2016-01-14 메디포스트(주) Fonction favorisant la pousse des cheveux de support de culture de cellules souches stimulées et son utilisation
EP3146973A4 (fr) * 2014-07-07 2018-01-10 Medipost Co., Ltd. Fonction favorisant la repousse des poils des cellules souches de petites tailles et son utilisation
EP4046625A4 (fr) * 2019-10-17 2023-10-11 Shanghai Seme Cell Technology Co., Ltd Utilisation d'un extrait de tissu adipeux acellulaire pour favoriser la croissance et la rétention des cheveux

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101311376B1 (ko) * 2011-12-05 2013-09-25 김용욱 지방유래 줄기세포 분리를 위한 원심분리방법 및 원심분리장치
KR101422559B1 (ko) 2012-03-09 2014-07-24 창원대학교 산학협력단 지방유래 줄기세포 배양액, 이의 제조방법, 및 이를 포함하는 발모촉진용 조성물
KR101764614B1 (ko) 2015-07-29 2017-08-04 주식회사 바이오솔루션 세포외기질 모사수의 제조방법 및 이를 포함하는 화장료 조성물

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005279934A1 (en) * 2004-08-30 2006-03-09 Theregen, Inc. Compositions and methods of promoting hair growth

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016006885A1 (fr) * 2014-07-07 2016-01-14 메디포스트(주) Fonction favorisant la pousse des cheveux de support de culture de cellules souches stimulées et son utilisation
EP3146973A4 (fr) * 2014-07-07 2018-01-10 Medipost Co., Ltd. Fonction favorisant la repousse des poils des cellules souches de petites tailles et son utilisation
EP4046625A4 (fr) * 2019-10-17 2023-10-11 Shanghai Seme Cell Technology Co., Ltd Utilisation d'un extrait de tissu adipeux acellulaire pour favoriser la croissance et la rétention des cheveux
US12514879B2 (en) 2019-10-17 2026-01-06 Shanghai Celleaf Biotechnology Co., Ltd. Use of acellular adipose tissue extract in promoting hair growth and retention

Also Published As

Publication number Publication date
KR101061464B1 (ko) 2011-09-01
WO2012091321A3 (fr) 2012-11-08

Similar Documents

Publication Publication Date Title
WO2012091321A2 (fr) Préparation pour favoriser la pousse des cheveux contenant un liquide de culture de cellules souches d'adipocyte et son procédé de préparation
Chunmeng et al. Skin: a promising reservoir for adult stem cell populations
DE69938653D1 (de) Isolierte stromalzellen zur verwendung in der behandlung von erkrankungen des zentralnervensystems
WO2012111997A2 (fr) Composition de thérapie génique permettant de prévenir ou de traiter une maladie immunitaire comprenant des cellules souches mésenchymateuses et des lymphocytes t immunorégulateurs comme principe actif
CN104622771A (zh) 一种含自体脂肪干细胞的生物美容面膜的制备方法及其产品
Stamegna et al. A unique method for the isolation of nasal olfactory stem cells in living rats
KR20160145778A (ko) 작은 크기 줄기세포의 발모 촉진능 및 이의 용도
WO2024101587A1 (fr) Composition cosmétique fonctionnelle
KR20150142287A (ko) 식물추출물을 포함하는 줄기세포 증식 촉진용 배지 조성물
CN109069588A (zh) 包含gdf11的组合物和其用途
WO2024085458A1 (fr) Composition cosmétique pour régénérer la peau ou améliorer la cicatrisation d'une plaie cutanée comprenant un produit fermenté d'extrait de gomme mastic en tant que principe actif, et son procédé de production
WO2019112161A2 (fr) Composition de milieu pour la prolifération cellulaire, la régénération de la peau et l'amélioration des rides comprenant, en tant que principe actif, un milieu conditionné de cellules souches pluripotentes, de cellules souches neurales ou de fibroblastes embryonnaires qui sont isolés à partir d'œufs aviaires
CN107460158A (zh) 一种诱导脐带间充质干细胞分化为形成胰岛素的方法
WO2011136434A1 (fr) Concentré de culture liquide de cellules souches dérivées d'un tissu adipeux humain ayant un effet de régénération de la peau ou d'atténuation des rides, et son utilisation
CN107164310A (zh) 一种体内重建毛囊的方法
Hoffman et al. Hair follicle-associated pluripotent (HAP) stem cells
CN109718251A (zh) 利用干细胞重建毛囊的生发方法及应用
KR20120096185A (ko) 지방줄기세포를 사용한 탈모치료 및 모발증식
CN108888634A (zh) 毛囊干细胞提取物冻干粉的制备方法及应用
CN112891517A (zh) 促进头皮毛囊再生的细胞因子组合物及其制备方法与应用
WO2025154961A1 (fr) Composition cosmétique comprenant un milieu de culture organoïde
WO2025009766A1 (fr) Souche de bifidobacterium animalis subsp. lactis et son utilisation pour améliorer l'état des cheveux ou du cuir chevelu
ATE478136T1 (de) Verfahren zur herstellung von netzhautneurozyten aus neutralen stammzellen aus dem irisgewebe und in dem verfahren hergestelltes netzhautneurozyt
KR20130116972A (ko) 타인지방줄기세포를 배양하여 만든 모발증식제재 및 이의 제조방법
CN107951825A (zh) 一种含有枸杞干细胞提取物的祛斑护肤品及其制备方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11852421

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11852421

Country of ref document: EP

Kind code of ref document: A2