WO2012103385A3 - Biocatalyseurs synthétisant des cellulases dérégulées - Google Patents

Biocatalyseurs synthétisant des cellulases dérégulées Download PDF

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Publication number
WO2012103385A3
WO2012103385A3 PCT/US2012/022800 US2012022800W WO2012103385A3 WO 2012103385 A3 WO2012103385 A3 WO 2012103385A3 US 2012022800 W US2012022800 W US 2012022800W WO 2012103385 A3 WO2012103385 A3 WO 2012103385A3
Authority
WO
WIPO (PCT)
Prior art keywords
biocatalysts
deregulated
cellulases
synthesizing
products
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/022800
Other languages
English (en)
Other versions
WO2012103385A2 (fr
Inventor
Patrick O'mullan
Jase Patel
Matthias Schmalisch
Branden Wolner
Philippa Reeder
Muhammad Abdul Ali
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qteros Inc
Original Assignee
Qteros Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/270,166 external-priority patent/US20120064592A1/en
Application filed by Qteros Inc filed Critical Qteros Inc
Publication of WO2012103385A2 publication Critical patent/WO2012103385A2/fr
Publication of WO2012103385A3 publication Critical patent/WO2012103385A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne de nouveaux biocatalyseurs de Clostridium phytofermentans qui dérégulent l'activité cellulase et produisent des rendements élevées de produits. La présente invention concerne en outre des procédés d'utilisation des biocatalyseurs pour dégrader un matériau organique et pour utilisation dans des processus industriels.
PCT/US2012/022800 2011-01-26 2012-01-26 Biocatalyseurs synthétisant des cellulases dérégulées Ceased WO2012103385A2 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201161436575P 2011-01-26 2011-01-26
US61/436,575 2011-01-26
US201161442120P 2011-02-11 2011-02-11
US61/442,120 2011-02-11
US13/270,166 2011-10-10
US13/270,166 US20120064592A1 (en) 2011-01-26 2011-10-10 Biocatalysts synthesizing deregulated cellulases

Publications (2)

Publication Number Publication Date
WO2012103385A2 WO2012103385A2 (fr) 2012-08-02
WO2012103385A3 true WO2012103385A3 (fr) 2012-12-06

Family

ID=46581412

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/022800 Ceased WO2012103385A2 (fr) 2011-01-26 2012-01-26 Biocatalyseurs synthétisant des cellulases dérégulées

Country Status (1)

Country Link
WO (1) WO2012103385A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116286560B (zh) * 2023-05-11 2023-08-22 东北农业大学 一株拉乌尔菌hc6及其低温生产2,3-丁二醇的应用
CN116590164B (zh) * 2023-06-28 2024-07-26 云南大学 一株毕赤克鲁维酵母fjy-4菌株及其应用
CN118389484B (zh) * 2024-06-27 2024-08-23 吉林大学 一种固定化双酶及其制备方法和应用

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5079154A (en) * 1988-03-30 1992-01-07 Kao Corporation Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase
KR20090098419A (ko) * 2008-03-14 2009-09-17 인하대학교 산학협력단 전기천공법을 이용한 프로피오니박테리움 아크네스의형질전환 조건의 최적화
US20100028966A1 (en) * 2008-07-28 2010-02-04 Jeffrey Blanchard Methods and Compositions for Improving The production Of Products In Microorganisms
US20100317087A1 (en) * 2006-08-31 2010-12-16 Iogen Energy Corporation Modified cellulases with increased thermostability, thermophilicity, and alkalophilicity
WO2011106576A2 (fr) * 2010-02-24 2011-09-01 Qteros, Inc. Procédés et compositions pour activité enzymatique améliorée dans micro-organismes en fermentation
US20110269201A1 (en) * 2010-04-30 2011-11-03 Qteros, Inc. Redirected bioenergetics in recombinant cellulolytic clostridium microorganisms

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5079154A (en) * 1988-03-30 1992-01-07 Kao Corporation Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase
US20100317087A1 (en) * 2006-08-31 2010-12-16 Iogen Energy Corporation Modified cellulases with increased thermostability, thermophilicity, and alkalophilicity
KR20090098419A (ko) * 2008-03-14 2009-09-17 인하대학교 산학협력단 전기천공법을 이용한 프로피오니박테리움 아크네스의형질전환 조건의 최적화
US20100028966A1 (en) * 2008-07-28 2010-02-04 Jeffrey Blanchard Methods and Compositions for Improving The production Of Products In Microorganisms
WO2011106576A2 (fr) * 2010-02-24 2011-09-01 Qteros, Inc. Procédés et compositions pour activité enzymatique améliorée dans micro-organismes en fermentation
US20110269201A1 (en) * 2010-04-30 2011-11-03 Qteros, Inc. Redirected bioenergetics in recombinant cellulolytic clostridium microorganisms

Also Published As

Publication number Publication date
WO2012103385A2 (fr) 2012-08-02

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