WO2012108669A3 - Aptamère d'adn spécifique d'ions uranyle - Google Patents

Aptamère d'adn spécifique d'ions uranyle Download PDF

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Publication number
WO2012108669A3
WO2012108669A3 PCT/KR2012/000889 KR2012000889W WO2012108669A3 WO 2012108669 A3 WO2012108669 A3 WO 2012108669A3 KR 2012000889 W KR2012000889 W KR 2012000889W WO 2012108669 A3 WO2012108669 A3 WO 2012108669A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna aptamer
specific dna
ion specific
uranyl ion
uranyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2012/000889
Other languages
English (en)
Korean (ko)
Other versions
WO2012108669A2 (fr
Inventor
하상수
김지수
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyung Hee University
Original Assignee
Kyung Hee University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyung Hee University filed Critical Kyung Hee University
Publication of WO2012108669A2 publication Critical patent/WO2012108669A2/fr
Publication of WO2012108669A3 publication Critical patent/WO2012108669A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un aptamère d'ADN spécifique d'ions uranyle. L'invention concerne également un aptamère d'ADN spécifique d'ions uranyle qui est lié à un support solide. L'invention concerne également un procédé d'isolement d'ions uranyle à partir d'un échantillon par l'utilisation de l'aptamère d'ADN spécifique d'ions uranyle. L'aptamère d'ADN de la présente invention se lie à des ions uranyles avec une spécificité élevée, si bien que l'uranium peut être efficacement extrait à partir d'un environnement marin et d'autres environnements de ce type.
PCT/KR2012/000889 2011-02-08 2012-02-07 Aptamère d'adn spécifique d'ions uranyle Ceased WO2012108669A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110010928A KR101238297B1 (ko) 2011-02-08 2011-02-08 우라닐 이온-특이적 ｄｎａ 앱타머
KR10-2011-0010928 2011-02-08

Publications (2)

Publication Number Publication Date
WO2012108669A2 WO2012108669A2 (fr) 2012-08-16
WO2012108669A3 true WO2012108669A3 (fr) 2012-12-20

Family

ID=46639044

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/000889 Ceased WO2012108669A2 (fr) 2011-02-08 2012-02-07 Aptamère d'adn spécifique d'ions uranyle

Country Status (2)

Country Link
KR (1) KR101238297B1 (fr)
WO (1) WO2012108669A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102248839B1 (ko) * 2019-12-09 2021-05-06 충북대학교 산학협력단 리튬에 특이적으로 결합하는 dna 앱타머 및 이의 용도
KR102328488B1 (ko) 2021-06-24 2021-11-17 세종대학교산학협력단 니켈 이온 및 코발트 이온 중 적어도 하나에 특이적으로 결합하는 앱타머
CN113773444B (zh) * 2021-09-08 2022-04-26 北京大学 一种在海水中回收微量铀的材料及方法
KR102378495B1 (ko) 2021-11-01 2022-03-23 세종대학교산학협력단 니켈 이온에 특이적으로 결합하는 앱타머
JP7840556B2 (ja) * 2022-01-19 2026-04-06 国立大学法人東京科学大学 海水ウラン捕集方法及びウラン原料の製造方法
KR102735103B1 (ko) 2022-10-18 2024-11-27 세종대학교산학협력단 망간 이온에 특이적으로 결합하는 앱타머

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100009031A (ko) * 2008-07-17 2010-01-27 김양훈 비소에 특이적으로 결합하는 ｄｎａ 앱타머 및 그의 용도

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9273345B2 (en) 2006-12-28 2016-03-01 Conceptual Mindworks, Inc. Methods and compositions for processes of rapid selection and production of nucleic acid aptamers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100009031A (ko) * 2008-07-17 2010-01-27 김양훈 비소에 특이적으로 결합하는 ｄｎａ 앱타머 및 그의 용도

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BREAKER, R. R. ET AL.: "DNA aptamers and DNA enzymes", CURRENT OPINION IN CHEMICAL BIOLOGY., vol. 1, no. 1, June 1997 (1997-06-01), pages 26 - 31 *
KIM, J. ET AL.: "Binding or uranyl ion by a DNA aptamer attached to a solid support", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS., vol. 21, no. 13, 6 May 2011 (2011-05-06), pages 4020 - 4022 *
LIU, J. ET AL.: "A catalytic beacon sensor for uranium with parts-per-trillion sensitivity and millionfold selectivity", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE, vol. 104, no. 7, 6 February 2007 (2007-02-06), pages 2056 - 2061 *
STOLTENBURG, R. ET AL.: "SELEX- a (r)evolutionary method to generate high-affinity nucleic acid ligands", BIOMOLECULAR ENGINEERING., vol. 24, no. 4, 16 June 2007 (2007-06-16), pages 381 - 403 *

Also Published As

Publication number Publication date
WO2012108669A2 (fr) 2012-08-16
KR101238297B1 (ko) 2013-03-04
KR20120090477A (ko) 2012-08-17

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