WO2012108745A2 - Composition et alimentation fonctionnelle pour traiter le cancer du cerveau comprenant un extrait de gleditsiae semen - Google Patents

Composition et alimentation fonctionnelle pour traiter le cancer du cerveau comprenant un extrait de gleditsiae semen Download PDF

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Publication number
WO2012108745A2
WO2012108745A2 PCT/KR2012/001079 KR2012001079W WO2012108745A2 WO 2012108745 A2 WO2012108745 A2 WO 2012108745A2 KR 2012001079 W KR2012001079 W KR 2012001079W WO 2012108745 A2 WO2012108745 A2 WO 2012108745A2
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Prior art keywords
brain cancer
composition
extract
brain
cancer
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Korean (ko)
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WO2012108745A3 (fr
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황성연
정경채
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Korea Bio Medical Science Institute Co Ltd
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Korea Bio Medical Science Institute Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/483—Gleditsia (locust)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the present invention relates to a novel use of extracts which are flakes. Specifically, the present invention relates to a therapeutic composition and functional food containing the extract as an active ingredient, which is a fragment showing excellent prophylactic or therapeutic efficacy against brain cancer.
  • Brain cancer refers to abnormally increased proliferation of any type of neuron, also referred to as primary brain cancer, or any other cancer that metastasizes to the central nervous system (CNS), also referred to as brain metastasis.
  • CNS central nervous system
  • Primary brain cancer can only occur in those cases where the nerve cells lose their protective ability to undergo apoptosis.
  • Examples of primary brain cancers include, but are not limited to, neuroma, astrocytoma, neuroblastoma, glioma, meningioma, oligodendrocyte, stromal blastoma, spinal cord tumor and neuromyoma.
  • Gliomas comprise about 60% of all primary CNS tumors and typically occur in the cerebral hemispheres of the brain, but can be found in other areas such as the visual nerve, brain stem or cerebellum. Gliomas can be classified into groups according to the type of glial cell from which they originated (Kornblith et al, (1986), Cancer: Principles and Practice of Oncology, 2nd Ed., DeVita, V., Hellman, S., Rosenberg , S, eds., JB Lippincott Company, Philadelphia, Chapter 41: Neoplasms of the Central Nervous System). The most common type of glioma is astrocytoma. These tumors develop from star-shaped glial cells called astroglia.
  • Astrocytes are assigned a rating according to their malignancy.
  • Low-grade astrocytomas also known as grade I and II astrocytomas
  • Mid-grade astrocytoma also known as grade III astrocytoma
  • Grade III astrocytoma is treated with radiation and some chemotherapy following surgery.
  • High-grade astrocytoma also known as grade IV astrocytoma
  • Grade IV astrocytomas are usually treated with a combination of radiation and chemotherapy following surgery.
  • Glioblastoma multiforme is a grade IV astrocytoma, which is the most malignant and lethal primary brain tumor (Id).
  • Id primary brain tumor
  • the benefit of treatment is minimal, and treatment can significantly reduce the quality of the patient's short-lived life.
  • CNS tumors represent the most common group of solid tumors in young patients, there is a clear need in the art for primary brain cancer prevention and treatment methods that overcome the disadvantages of the above-mentioned conventional approaches.
  • Brain tumors are a secondary-induced cause of cancer-related death in infants, with approximately 25% of all such deaths, with current therapies in which most of these infants die within the first year of diagnosis.
  • Brain metastasis results from primary tumors elsewhere in the body, including, but not limited to, lung cancer (both small and non-small cell), breast cancer, colorectal cancer, prostate cancer, melanoma and pancreatic cancer. Particularly in patients with metastatic breast cancer, the incidence of brain metastasis is diagnosed at a rate of 10-20% (Tyson, R.M. et al, Therapy 3 (1), 97-112 (2006)).
  • Breast cancer is the second leading cause of cancer-related death in women, and nearly all deaths from breast cancer are due to metastatic disease with brain metastases found in 30% of patients at autopsy.
  • Standard modes of treatment include surgical resection, chemotherapy and radiation therapy, in particular whole-brain radiation therapy (WBRT) and stereotactic radiosurgery or combinations thereof.
  • Surgery for brain metastases can improve survival, especially in patients with a single lesion. Surgery, however, may not be possible in terms of multiple lesions, lesions that are difficult to surgically approach, or inability to withstand surgery. In breast cancer, 50% of patients with brain cancer metastases have multiple metastases, making them less suitable as surgical candidates.
  • WBRT can improve intermediate survival over untreated and as an adjuvant for surgery, it reduces the rate of relapse and dying nerve death, but does not change survival or level of action.
  • SRS provides a method for treating brain metastases that cannot be surgically resected by location or patient symptoms.
  • SRS provides an improvement in quality of life but does not provide survival benefits, except for patients with a single metastasis.
  • the use of chemotherapy in the treatment of brain metastases is hampered by the impossibility of high molecular weight compounds, limiting most chemotherapeutic agents across the blood brain barrier. This is reflected in the fact that chemosensitive tumors, such as most metastatic breast carcinomas, often exhibit a complete systemic response to chemotherapy simultaneously with tumor progression in the brain.
  • this initial resistance to the use of chemotherapy in the treatment of brain metastases has recently changed based on observations in animal models and human brain tumor autopsies, and metastatic lesions cause blood brain barrier insufficiency, whereby chemotherapy Drugs can always enter the tumor.
  • Radiation therapy combined with chemotherapy has evolved from a first-line approach in the treatment of brain metastases.
  • Brain cancer is a generic term for primary brain cancer that develops in the brain tissue and the envelope that surrounds the brain, and secondary brain cancer that has metastasized from cancer that occurs in the skull or other parts of the body.
  • Such brain cancers are often distinguished from cancers occurring in other organs.
  • lung, stomach, and breast cancers occur in one or two types of organs, and their properties are the same and similar.
  • the brain develops a wide variety of cancers. For example, glioblastoma multiforme, malignant gliomas, lymphomas, germ cell tumors, metastatic tumors, and the like.
  • glioma especially glioblastoma multiforme (GBM)
  • GBM glioblastoma multiforme
  • Gleditsiae Semen is the seed of the juniper tree ( Gledisia japonica Miquel var.koraiensis Nakai). It is a herbal medicine used in various folk remedies such as controlling wind and improving bowel function. Thorn is called a sculptor, and it has the effect of sowing, drainage, etc., used in various boils. Overwintering is also possible in central Korea. Gleditsia sinensis Lam. Is a herb used in various folk remedies such as improving intestinal function in East Asia.
  • the present inventors completed the present invention by confirming that the extract of the sculptor can effectively kill brain cancer cells while studying the herbal medicine for sculptor.
  • the present invention provides a composition for the prevention and treatment of brain cancer comprising an active ingredient extracted with sculpting organic solvent.
  • the organic solvent is preferably ethanol.
  • the present invention provides a functional food for preventing brain cancer containing ethanol extract as an active ingredient in pieces containing food acceptable food additives.
  • the present invention provides a composition for the prevention and treatment of brain cancer containing Gleditsiae Semen ethanol extract as an active ingredient.
  • the compositions of the present invention comprise extracts that are flakes as active ingredients and may further comprise pharmaceutically acceptable carriers or diluents.
  • the ethanol extract is preferably extracted for 24 hours at 50 °C, wherein the ethanol extract is more preferably dried and concentrated at 45 °C reduced pressure conditions, the ethanol is Most preferred is 95%.
  • the composition for the prevention and treatment of brain cancer of the present invention is selected from the primary brain cancer crowd consisting of neuroma, astrocytoma, neuroblastoma, glioma, meningioma, oligodendrocyte tumor, stromal blastoma, spinal cord tumor and neuromyoma It is preferably applied to brain cancer.
  • the composition for preventing and treating brain cancer of the present invention, preferably comprises a pharmaceutically acceptable carrier or diluent.
  • the "pharmaceutically acceptable carrier” is a pharmaceutically acceptable substance such as a liquid or solid filler, diluent, excipient or solvent which serves to transport the active ingredient from one organ or part of the body to another organ or part of the body. , Composition or vehicle.
  • composition for treating brain cancer of the present invention may be prepared as a medicament by adding one or more pharmaceutically acceptable carriers together with the active ingredient.
  • the carrier may include, but is not limited to, saline, buffered saline, water, glycerol and ethanol, and any suitable agent known in the art (Remingtons's Pharmaceutical Science (Recent Edition), Mack Publishing Company, Easton PA) may be used. .
  • Formulations for pharmaceutical formulation of the extract of the present invention can be administered orally at the time of clinical administration and can be used in the form of general pharmaceutical formulations, when formulated, commonly used fillers, extenders, binders, wetting agents, disintegrants And diluents such as surfactants or excipients.
  • Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc.
  • liquid preparations for oral use include suspensions, solvents, emulsions, and syrups.
  • various excipients may be included, such as wetting sweeteners, fragrances, preservatives and the like.
  • composition of the present invention may be administered in various parenteral formulations during actual clinical administration, and solid preparations include tablets, pills, powders, granules, capsules, and the like.
  • solid preparations include tablets, pills, powders, granules, capsules, and the like.
  • excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included.
  • preparations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories.
  • non-aqueous solvent and the suspension solvent propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used.
  • base of the suppository witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.
  • calcium or vitamin D 3 may be added to enhance the efficacy of the treatment.
  • Such compositions may be presented in unit-dose (single) or multi-dose (several) containers, such as sealed ampoules and vials, and immediately before use, sterile liquid carriers such as injectable water. Can be stored under freeze-drying conditions requiring only the addition of. Immediate injection solutions and suspensions can be prepared from sterile powders, granules and tablets.
  • the formulations of the present invention can be applied differently depending on the age, sex, condition of the subject, the absorption of the active ingredient in the body, the inactivation rate and excretion rate, the drug used in combination.
  • the invention also includes formulations of dosage units.
  • the formulations are present in individual dosage forms, such as tablets, coated tablets, capsules, pills, suppositories, and ampoules, wherein the amount of active compound in the drug corresponds to the fraction or multiple of the individual dosage.
  • Dosage units may contain, for example, one, two, three or four times the individual dosage, or 1/2, 1/3 or 1/4 times.
  • the individual dosages preferably contain an amount in which the active compound is administered at one time, which usually corresponds to all, 1/2, 1/3 or 1/4 times the daily dosage.
  • extract refers to an active ingredient isolated from natural products.
  • the extract may be obtained by an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes an extract, a dry powder thereof, or any form formulated using the same.
  • ethanol extract of Sculpture killed 99.8% of U-87 MG brain cancer cells at 100 ⁇ g / ml and the EC 50 (half maximal effective concentration) was 24.412 ⁇ g / ml.
  • the above results demonstrate that the extract, a fragment of the present invention, has excellent killing activity of U-87 MG cells and further has brain cancer treatment and prophylactic activity.
  • prevention means any action that inhibits or delays the onset of brain cancer by administration of the composition.
  • treatment means any action that improves or advantageously alters the symptoms of brain cancer by administration of the composition.
  • Extract which is a flake in the present invention may be extracted by using water, an organic solvent, or a mixed solvent thereof. Preferably it is extracted using an organic solvent, in particular ethanol.
  • the extracted solution can be used directly or can be concentrated and / or dried.
  • methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof may be used and extracted by room temperature or warming under conditions where the active ingredient of the herbal medicine is not destroyed or minimized.
  • the degree of extraction and loss of the active ingredient of the drug may vary, so select an appropriate organic solvent.
  • the extraction method is not particularly limited, and examples thereof include cold needle extraction, ultrasonic extraction, reflux cooling extraction, and the like.
  • Filtration is a process of removing the suspended solid particles from the extract, it may be used to filter the particles using cotton, nylon or the like, or may be used, such as ultrafiltration, cryofiltration, centrifugal separation, but is not limited thereto.
  • Concentration of the extract may be used, such as concentrated under reduced pressure, reverse osmosis concentration.
  • the drying step after concentration includes freeze drying, vacuum drying, hot air drying, spray drying, reduced pressure drying, foam drying, high frequency drying, infrared drying, and the like. If desired, a process of grinding the final dried extract may be added.
  • the extract can perform an additional fractionation process.
  • the extract is suspended in distilled water to obtain a nonpolar solvent soluble layer by extraction and separation with a nonpolar organic solvent such as hexane, ether, dichloromethane, chloroform, ethyl acetate, or a mixed solvent thereof. It can be used by concentrating and / or drying it.
  • a nonpolar organic solvent such as hexane, ether, dichloromethane, chloroform, ethyl acetate, or a mixed solvent thereof. It can be used by concentrating and / or drying it.
  • the term "pharmaceutically acceptable salts” means salts derived from pharmacologically or physiologically acceptable inorganic acids, organic acids and bases.
  • suitable acid include hydrochloric acid, bromic acid, sulfuric acid, nitric acid, perchloric acid, fumaric acid, maleic acid, phosphoric acid, glycolic acid, lactic acid, salicylic acid, succinic acid, toluene-p-sulfonic acid, tartaric acid, acetic acid, citric acid, methanesulfonic acid, Formic acid, benzoic acid, malonic acid, naphthalene-2-sulfonic acid, benzenesulfonic acid, and the like.
  • Salts derived from suitable bases may include alkali metals such as sodium, alkaline earth metals such as magnesium, ammonium and the like.
  • the pharmaceutical composition for preventing and treating brain cancer diseases of the present invention comprises 0.1 to 50% by weight of the extract or compound based on the total weight of the composition.
  • the composition does not increase the efficacy, but may include additional ingredients that are commonly used in the pharmaceutical composition to improve the smell, taste, time and the like.
  • the composition adds inorganic and organic additives such as vitamins B1, B2, B6, C, E, niacin, carnitine, betaine, folate pantothenic acid, biotin, zinc, iron, calcium, chromium, magnesium, and mixtures thereof. It can be included as.
  • the composition may include a substance having a therapeutic activity against brain cancer used alone or used in the past.
  • the term "patient” refers to humans and horses, sheep, pigs, goats, camels, who have a disease caused by brain cancer and its direct and indirect causes, and whose symptoms can be improved by administering the composition of the present invention. Means antelope, dog and other animals.
  • a composition comprising an extract which is a fragment of the invention, the above-mentioned brain cancer can be effectively prevented and treated.
  • the composition of the present invention can be administered in parallel with existing brain cancer therapeutics.
  • the term "administration” means introducing a predetermined substance into a patient by any suitable method, and the route of administration of the composition of the present invention is oral or parenteral via any general route as long as the target tissue can be reached. May be administered.
  • the composition may be administered by any device in which the active agent may migrate to the target cell.
  • composition of the present invention is administered in a pharmaceutically effective amount.
  • pharmaceutically effective amount means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and an effective dose level refers to a patient's sexually transmitted disease, age, severity, and drug activity.
  • the compositions of the present invention may be administered as individual therapeutic agents or in combination with other therapeutic agents and may be administered sequentially or simultaneously with conventional therapeutic agents. It may be single or multiple doses.
  • the method of administering the composition comprising the extract or compound prepared according to the preparation method of the present invention is preferably oral administration or intravenous administration.
  • the effective dose is oral administration, it is usually 1 to 1 time per adult. 500 mg / kg is preferred, and in the case of intravenous administration, 1 to 100 mg / kg is preferred, and may be administered 2-3 times a day.
  • Dosage levels for a particular patient may vary depending on sex, age, health condition, diet, time of administration, method of administration, drug mixture, the condition of the patient, and the extent of the onset of neurological disease.
  • the present invention provides a functional food for preventing brain cancer containing Gleditsiae Semen ethanol extract containing a food acceptable food supplement additive as an active ingredient.
  • Functional food of the present invention preferably contains 0.1 to 5% by weight, more preferably 1% by weight of the ethanol extract relative to the total weight.
  • the present invention As a result of injecting the extract of the present invention into U-87 MG brain cancer cells, it was confirmed that the U-87 MG brain cancer cells die (see Fig. 1 ). Therefore, it can be seen that the extract of the fragment has a brain cancer treatment effect.
  • the present invention was completed by preparing a functional food for preventing brain cancer containing the extract as an active ingredient (see Preparation Example 2 ).
  • the extract of the present invention inhibits the growth of brain cancer cells and induces apoptosis. Therefore, the composition for treating brain cancer according to the present invention will be very effective in the treatment of brain cancer patients.
  • FIG. 1 is a result of Alamar Blue analysis to determine the effect of the introduction of fragments in human brain cancer U-87 MG cells on the growth of brain cancer cells.
  • the horizontal axis of the figure shows the concentration of the extract, the vertical axis shows the survival rate of brain cancer cells.
  • FIG. 2 is a result of Alamar Blue analysis to determine the effect of the introduction of turmeric extract on the growth of brain cancer cells in human brain cancer U-87 MG cells.
  • the horizontal axis of the figure shows the concentration of the extract, the vertical axis shows the survival rate of brain cancer cells.
  • Alamar Blue analysis was performed on U-87 MG, a human brain cancer cell.
  • the Alamar Blue assay is a modified form of the MTT assay, in which a specific enzyme degrades a living cell and then measures the fluorescence intensity of the product as the compound breaks down to determine the relative number of living cells after treatment. I am an experimental method. It will be described in more detail below.
  • U-87 MG cells a brain cancer cell line used in the present invention, were distributed from the Korean Cell Line Bank (KCLB) and used for experiments.
  • MEM Minimum Essential Medium
  • FBS fetal bovine serum, fetal bovine serum
  • HEPES 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid
  • the extract of the fragment extracted in Example 1 confirmed the cell growth inhibitory effect on U-87 MG brain cells. Specifically, 8.0 x 10 per each well in a 96 well plate 3 The fragments were seeded in U-87 MG cells and dissolved in dimethyl sulfoxide (DMSO). When the ethanol extract was treated at concentrations of 0 to 100 ⁇ g / ml (specifically, concentrations of 0, 3.125, 6.25, 12.5, 25, 50 and 100 ⁇ g / ml, respectively) for 48 hours, the degree of inhibition of cell growth was confirmed. (Table 1).
  • Table 1 describes the relative cell numbers of brain cancer cells after 48 hours according to the concentration of each fragment, based on the number of survival rates of brain cancer cells of the control group that was not treated with fragments.
  • the extract, a fragment of the present invention demonstrates good U-87 MG cell killing activity and further has brain cancer treatment and prophylactic activity.
  • Sculpture used in the present invention was widely used as a medicinal herb was determined that there is no problem in stability, but the oral administration and intraperitoneal toxicity experiments were carried out to confirm this.
  • Acute toxicity test was performed using 6-week-old SPF SD rats. Two animals per group were suspended orally administered at a dose of 5 g / kg, each of which was an extract of Example 1 of the present invention, suspended in 0.5% methylcellulose solution. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed. Hematological and hematological examinations were performed, and autopsy was performed to observe abdominal and thoracic organ abnormalities.
  • Turmeric is a substance that shows anticancer activity against various kinds of cancers such as lung cancer, uterine cancer and breast cancer.
  • the present inventors confirmed the effectiveness of the extract, which is a fragment of the present invention, by checking the extent to which the turmeric affects the growth of brain cancer cells.
  • the turmeric extract was confirmed the growth inhibition of the U-87 MG cells in the same manner as in Example 2, and the results are shown in Table 2 and FIG.
  • turmeric hardly reduced the growth of brain cancer cells even when the treatment concentration was high.
  • turmeric widely known as an anticancer substance, has almost no brain cancer treatment efficacy, whereas the extract, which is a fragment of the present invention, has excellent U-87 MG cell killing activity, and further shows that it has brain cancer treatment and prophylactic activity.
  • the present inventors have confirmed that the brain cancer treatment effect of the sculpted extract is excellent through the above embodiment to prepare a brain cancer treatment containing the sculpted extract as an active ingredient as follows.
  • the preparation of the following therapeutic agent can be used for the application of the health food as well as the therapeutic agent.
  • Root Extract 35%, Vitamin C 10%, Vitamin D 3 0.001%, Manganese Sulfate 0.1%
  • the inventors have confirmed that the extract extract is excellent in the brain cancer treatment activity through the above embodiment to prepare a functional food containing it as an active ingredient as follows.
  • Chewing gum was prepared using conventional methods using the composition and content of 20% gum base, 76.36 to 76.76% sugar, 0.24 to 0.64% flake extract, 1% fruit flavor, and 2% water.
  • composition and content of the sugar 34.36 ⁇ 34.76%, cocoa butter 34%, cocoa mass 15%, cocoa powder 15%, lecithin 0.5%, vanilla flavor 0.5%, flake extract 0.24 ⁇ 0.64% using conventional methods Chocolate was prepared.

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Abstract

L'invention concerne une nouvelle utilisation d'un extrait de Gleditsiae Semen et concerne notamment une composition pour prévenir et guérir le cancer du cerveau, la composition comprenant un extrait éthanolique de Gleditsiae Semen en tant qu'ingrédient actif, et une alimentation fonctionnelle pour prévenir le cancer du cerveau, l'alimentation fonctionnelle comprenant l'extrait éthanolique de Gleditsiae Semen, qui comprend un additif alimentaire cytologiquement acceptable en tant qu'ingrédient actif. La composition et l'alimentation fonctionnelle pour traiter le cancer du cerveau selon la présente invention sont efficaces pour inhiber la croissance et provoquer la mort des cellules cancéreuses; cela permet de les utiliser efficacement pour prévenir et guérir le cancer du cerveau.
PCT/KR2012/001079 2011-02-11 2012-02-13 Composition et alimentation fonctionnelle pour traiter le cancer du cerveau comprenant un extrait de gleditsiae semen Ceased WO2012108745A2 (fr)

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KR10-2011-0012218 2011-02-11
KR1020110012218A KR20120092279A (ko) 2011-02-11 2011-02-11 조각인 추출물을 포함하는 뇌암 치료용 조성물 및 건강 기능성 식품

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WO2014077433A1 (fr) * 2012-11-16 2014-05-22 주식회사 한국전통의학연구소 Composition contenant de l'extrait de semen gleditsiae utilisable pour traiter le cancer du rein et en tant qu'aliment fonctionnel
WO2014077434A1 (fr) * 2012-11-16 2014-05-22 주식회사 한국전통의학연구소 Composition contenant de l'extrait de semen gleditsiae utilisable pour traiter le cancer du pancréas et en tant qu'aliment fonctionnel

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KR0160107B1 (ko) * 1995-06-14 1998-12-01 최영태 조각자나무로부터 추출한 생약 성분으로 된 항균제
KR101008922B1 (ko) * 2008-04-04 2011-01-17 로드바이오(주) 조각자 가시 추출물을 포함하는 대장암 예방 또는 치료용조성물
CA2721072A1 (fr) * 2008-04-11 2009-10-15 Bionovo, Inc. Procedes anticancereux utilisant des extraits de gleditsia sinensis lam

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