WO2012109578A3 - Souches de clostridium thermocellum pour production améliorée d'éthanol et leur procédé d'utilisation - Google Patents

Souches de clostridium thermocellum pour production améliorée d'éthanol et leur procédé d'utilisation Download PDF

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Publication number
WO2012109578A3
WO2012109578A3 PCT/US2012/024713 US2012024713W WO2012109578A3 WO 2012109578 A3 WO2012109578 A3 WO 2012109578A3 US 2012024713 W US2012024713 W US 2012024713W WO 2012109578 A3 WO2012109578 A3 WO 2012109578A3
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WO
WIPO (PCT)
Prior art keywords
thermocellum
clostridium thermocellum
hydrogenases
pfl
ethanol production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/024713
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English (en)
Other versions
WO2012109578A2 (fr
Inventor
Adam Guss
Lee R. Lynd
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dartmouth College
Original Assignee
Dartmouth College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dartmouth College filed Critical Dartmouth College
Publication of WO2012109578A2 publication Critical patent/WO2012109578A2/fr
Publication of WO2012109578A3 publication Critical patent/WO2012109578A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0067—Oxidoreductases (1.) acting on hydrogen as donor (1.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Selon l'invention, le Clostridium thermocellum est une bactérie anaérobie thermophile qui se spécialise dans la solubilisation et la fermentation rapides de cellulose cristalline en des produits qui comprennent de l'éthanol. Des mutants de délétion de C. thermocellum ont été construits pour éliminer des activités de la lyase pyruvate-formiate (pfl) et de l'hydrogénase. C. thermocellum Apfl ne synthétise plus de formiate. Les mutants pfl produisent moins d'acétate que la souche parentale. Le mutant, qui n'exprime pas l'une des protéines de maturation requises pour la production d'hydrogénases fonctionnelles dans C. thermocellum, présente une production réduite d'acétate par comparaison avec des souches normales.
PCT/US2012/024713 2011-02-11 2012-02-10 Souches de clostridium thermocellum pour production améliorée d'éthanol et leur procédé d'utilisation Ceased WO2012109578A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161442109P 2011-02-11 2011-02-11
US61/442,109 2011-02-11

Publications (2)

Publication Number Publication Date
WO2012109578A2 WO2012109578A2 (fr) 2012-08-16
WO2012109578A3 true WO2012109578A3 (fr) 2012-11-22

Family

ID=46639223

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/024713 Ceased WO2012109578A2 (fr) 2011-02-11 2012-02-10 Souches de clostridium thermocellum pour production améliorée d'éthanol et leur procédé d'utilisation

Country Status (1)

Country Link
WO (1) WO2012109578A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11549103B2 (en) 2014-01-30 2023-01-10 Lanzatech Nz, Inc. Recombinant microorganisms and methods of use thereof

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2534880C1 (ru) * 2013-09-23 2014-12-10 Федеральное государственное бюджетное учреждение науки Институт цитологии и генетики Сибирского отделения Российской академии наук (ИЦиг СО РАН) ШТАММ БАКТЕРИИ Geobacillus stearothermophilus - ПРОДУЦЕНТ БИОЭТАНОЛА
CN109055459A (zh) * 2018-08-17 2018-12-21 中国科学院青岛生物能源与过程研究所 用于木质纤维素的全菌糖化方法
CN110540982B (zh) * 2019-09-30 2021-11-02 江南大学 一种提高梭热杆菌纤维素酶酶活的发酵方法
CN114717174B (zh) * 2022-04-26 2023-10-20 苏州聚维元创生物科技有限公司 一种产高品质还原糖的工程菌株、构建方法及其应用
CN115029264B (zh) * 2022-06-14 2023-06-09 中国科学院青岛生物能源与过程研究所 一株耐酸热纤梭菌及其降解木质纤维素的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5770435A (en) * 1995-11-02 1998-06-23 University Of Chicago Mutant E. coli strain with increased succinic acid production
US6280986B1 (en) * 1997-12-01 2001-08-28 The United States Of America As Represented By The Secretary Of Agriculture Stabilization of pet operon plasmids and ethanol production in bacterial strains lacking lactate dehydrogenase and pyruvate formate lyase activities
WO2009079584A1 (fr) * 2007-12-17 2009-06-25 The Trustees Of Dartmouth College Modification d'activités d'hydrogénase dans des bactéries thermophiles pour amplifier la production d'éthanol
US20090221049A1 (en) * 2005-10-31 2009-09-03 Shaw Iv Arthur Josephus Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5770435A (en) * 1995-11-02 1998-06-23 University Of Chicago Mutant E. coli strain with increased succinic acid production
US6280986B1 (en) * 1997-12-01 2001-08-28 The United States Of America As Represented By The Secretary Of Agriculture Stabilization of pet operon plasmids and ethanol production in bacterial strains lacking lactate dehydrogenase and pyruvate formate lyase activities
US20090221049A1 (en) * 2005-10-31 2009-09-03 Shaw Iv Arthur Josephus Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol
WO2009079584A1 (fr) * 2007-12-17 2009-06-25 The Trustees Of Dartmouth College Modification d'activités d'hydrogénase dans des bactéries thermophiles pour amplifier la production d'éthanol

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JOSE JOAO MANSURE ET AL.: "Desulfovibrio vulgaris Hildenborough HydE and HydG interact with the HydA subunit of the [FeFe] hydrogenase", BIOTECHNOLOGY LETTERS., vol. 30, no. 10, 18 June 2008 (2008-06-18), pages 1765 - 1769 *
SHAWN E. MCGLYNN ET AL.: "In vitro activation of [FeFe] hydrogenase: new insights into hydrogenase maturation", JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY., vol. 12, no. 4, May 2007 (2007-05-01), pages 443 - 447 *
SHITAL A. TRIPATHI ET AL.: "Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 76, no. 19, 6 August 2010 (2010-08-06), pages 6591 - 6599 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11549103B2 (en) 2014-01-30 2023-01-10 Lanzatech Nz, Inc. Recombinant microorganisms and methods of use thereof

Also Published As

Publication number Publication date
WO2012109578A2 (fr) 2012-08-16

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