WO2012118349A3 - 제대혈 ｃｄ14 양성 단핵세포로부터 자연살해세포 분화 및 증식 방법 - Google Patents

제대혈 ｃｄ14 양성 단핵세포로부터 자연살해세포 분화 및 증식 방법 Download PDF

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WO2012118349A3
WO2012118349A3 PCT/KR2012/001587 KR2012001587W WO2012118349A3 WO 2012118349 A3 WO2012118349 A3 WO 2012118349A3 KR 2012001587 W KR2012001587 W KR 2012001587W WO 2012118349 A3 WO2012118349 A3 WO 2012118349A3
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cells
natural killer
positive
cord blood
differentiation
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English (en)
French (fr)
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WO2012118349A2 (ko
WO2012118349A9 (ko
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최인표
윤석란
김미정
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Korea Research Institute of Bioscience and Biotechnology KRIBB
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Korea Research Institute of Bioscience and Biotechnology KRIBB
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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/51—Umbilical cord; Umbilical cord blood; Umbilical stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/15—Natural-killer [NK] cells; Natural-killer T [NKT] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C12N5/0087—Purging against subsets of blood cells, e.g. purging alloreactive T cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2315—Interleukin-15 (IL-15)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2321—Interleukin-21 (IL-21)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
    • C12N2506/115—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells from monocytes, from macrophages

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
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  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 제대혈로부터 효율적인 자연살해세포(natural killer cell; NK 세포)의 증식 및 분화 방법에 관한 것으로, 보다 상세하게는 1) 제대혈 유래 단핵세포로부터 CD3, CD34, CD56 및 CD19 양성세포를 제거하여 CD3-CD34-CD56-CD19-세포를 제조하는 단계; 2) 상기 CD3-CD34-CD56-CD19-세포로부터 CD14 음성세포를 제거하여 CD3-CD34-CD56-CD19-CD14+세포를 제조하는 단계; 및 3) 상기 CD3-CD34-CD56-CD19-CD14+세포에 IL-15 및 IL-21을 혼합처리한 후 배양하는 단계를 포함하는 제대혈로부터 효율적으로 자연살해세포를 증식 및 분화시키는 방법에 관한 것이다. 본 발명은 CD14 양성세포로부터 NK 세포를 유도함으로써 조혈 줄기 세포로부터 NK 세포를 유도하는 방법에 비해 순도가 높은 NK 세포를 단시간에 얻을 수 있고, 또한 우수한 세포 살상능 활성을 가진 NK 세포를 단시간에 유도하여 암세포 살상 기능이 있는 NK 세포를 분화시킬 수 있으므로 항암세포 치료에 유용하게 이용될 수 있다.
PCT/KR2012/001587 2011-03-03 2012-03-02 제대혈 ｃｄ14 양성 단핵세포로부터 자연살해세포 분화 및 증식 방법 Ceased WO2012118349A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0018949 2011-03-03
KR1020110018949A KR101447546B1 (ko) 2011-03-03 2011-03-03 제대혈 ｃｄ14 양성 단핵세포로부터 자연살해세포 분화 및 증식 방법

Publications (3)

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WO2012118349A2 WO2012118349A2 (ko) 2012-09-07
WO2012118349A9 WO2012118349A9 (ko) 2012-10-11
WO2012118349A3 true WO2012118349A3 (ko) 2012-11-29

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PCT/KR2012/001587 Ceased WO2012118349A2 (ko) 2011-03-03 2012-03-02 제대혈 ｃｄ14 양성 단핵세포로부터 자연살해세포 분화 및 증식 방법

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KR (1) KR101447546B1 (ko)
WO (1) WO2012118349A2 (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6775426B2 (ja) * 2014-03-07 2020-10-28 エメルセル エスエーエス 臍帯血由来のプールｎｋ細胞並びにがん及び慢性感染症の治療のためのこれらの用途
WO2016122014A1 (ko) * 2015-01-27 2016-08-04 한국생명공학연구원 자연살해세포의 대량생산 방법 및 상기 방법으로 수득된 자연살해세포의 항암제로서의 용도
US12398371B2 (en) 2015-01-27 2025-08-26 Korea Research Institute Of Bioscience Method for mass producing natural killer cell and use of natural killer cell obtained by the method as anti-cancer agent
WO2017003153A1 (ko) * 2015-06-29 2017-01-05 주식회사 녹십자랩셀 제대혈 단핵세포 또는 이로부터 유래된 세포로부터 자연살해세포를 제조하는 방법
US12486492B2 (en) 2020-03-05 2025-12-02 Korea Research Institute Of Bioscience Method for producing memory-like NK cells with ability to express higher levels of NCRs, cytotoxicity, and IFN-γ than NK cells in human peripheral blood
KR20230078541A (ko) 2021-11-23 2023-06-02 재단법인 아산사회복지재단 이중특이성항체를 이용한 자연살해세포 대량증식 방법
CN114058584B (zh) * 2022-01-07 2022-07-01 山东省齐鲁干细胞工程有限公司 一种临床用自然杀伤细胞的制备方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100045704A (ko) * 2008-10-24 2010-05-04 주식회사 메디셀 제대혈로부터 효율적인 자연살해세포의 증식 및 분화 방법

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100045704A (ko) * 2008-10-24 2010-05-04 주식회사 메디셀 제대혈로부터 효율적인 자연살해세포의 증식 및 분화 방법

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DALLE ET AL.: "Characterization of cord blood natural killer cells: implications for transplantation and neonatal infections", PEDIATRIC RESEARCH, vol. 57, no. 5, 2005, pages 649 - 655 *
GIULIANI ET AL.: "Generation of a novel regulatory NK cell subset from peripheral blood CD34+ progenitors promoted by membrane-bound IL-15", PLOS ONE, vol. 3, no. ISSUE, 21 May 2008 (2008-05-21), pages 1 - 16 *
STRENGELL ET AL.: "IL-21 in synergy with IL-15 or IL-18 enhance IFN-gamma production in human NK an T cells", THE JOURNAL OF IMMUNOLOGY, vol. 170, 2003, pages 5464 - 5469 *

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KR101447546B1 (ko) 2014-10-08
KR20120100207A (ko) 2012-09-12
WO2012118349A2 (ko) 2012-09-07
WO2012118349A9 (ko) 2012-10-11

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