WO2012120176A3 - Proteínas recombinantes con efecto antitumoral - Google Patents

Proteínas recombinantes con efecto antitumoral Download PDF

Info

Publication number
WO2012120176A3
WO2012120176A3 PCT/ES2012/070150 ES2012070150W WO2012120176A3 WO 2012120176 A3 WO2012120176 A3 WO 2012120176A3 ES 2012070150 W ES2012070150 W ES 2012070150W WO 2012120176 A3 WO2012120176 A3 WO 2012120176A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant proteins
antitumor effects
vector
sequence
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/ES2012/070150
Other languages
English (en)
French (fr)
Other versions
WO2012120176A2 (es
Inventor
María VILANOVA BRUGUES
Antoni BENITO MUNDET
Marc RIBÓ PANOSA
Jessica CASTRO GALLEGOS
Pere TUBERT JUHE
Anna VERT COMPANY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitat de Girona
Original Assignee
Universitat de Girona
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitat de Girona filed Critical Universitat de Girona
Publication of WO2012120176A2 publication Critical patent/WO2012120176A2/es
Publication of WO2012120176A3 publication Critical patent/WO2012120176A3/es
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/27—Endoribonucleases producing 3'-phosphomonoesters (3.1.27)
    • C12Y301/27005—Pancreatic ribonuclease (3.1.27.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención proporciona una proteína recombinante con actividad ribonucleasa que comprende una secuencia derivada de la ribonucleasa pancreática humana (RNasa-PH) de secuencia SEC ID NO: 37 fusionada a través de su extremo N-terminal a una secuencia de localización nuclear convencional, así como una molécula de ADN recombinante que codifica para esta proteína, un vector que comprende dicha molécula de ADN y una célula que comprende dicho vector. Adicionalmente la presente invención está referida a un procedimiento para la obtención de la proteína recombinante y el uso de la misma como agente antitumoral.
PCT/ES2012/070150 2011-03-10 2012-03-08 Proteínas recombinantes con efecto antitumoral Ceased WO2012120176A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP201130324 2011-03-10
ES201130324A ES2388020B1 (es) 2011-03-10 2011-03-10 Proteínas recombinantes con efecto antitumoral.

Publications (2)

Publication Number Publication Date
WO2012120176A2 WO2012120176A2 (es) 2012-09-13
WO2012120176A3 true WO2012120176A3 (es) 2012-11-01

Family

ID=46798596

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2012/070150 Ceased WO2012120176A2 (es) 2011-03-10 2012-03-08 Proteínas recombinantes con efecto antitumoral

Country Status (2)

Country Link
ES (1) ES2388020B1 (es)
WO (1) WO2012120176A2 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3679143A1 (en) * 2017-09-08 2020-07-15 Life Technologies Corporation Methods for improved homologous recombination and compositions thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004284977A (ja) * 2003-03-20 2004-10-14 Masakazu Ueda ヒト生理活性物質とヒト蛋白質合成阻害物質との挿入融合体
US20050261232A1 (en) * 2004-04-13 2005-11-24 Quintessence Biosciences, Inc. Non-natural ribonuclease conjugates as cytotoxic agents
WO2006138558A2 (en) * 2005-06-16 2006-12-28 Wisconsin Alumni Research Foundation Cytotoxic ribonuclease variants
JP2010180174A (ja) * 2009-02-06 2010-08-19 Tokyo Univ Of Science 膵癌の診断方法および膵癌の診断用キット
WO2011161260A1 (en) * 2010-06-25 2011-12-29 Adamed Sp. Z O.O. Anticancer fusion protein

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004284977A (ja) * 2003-03-20 2004-10-14 Masakazu Ueda ヒト生理活性物質とヒト蛋白質合成阻害物質との挿入融合体
US20050261232A1 (en) * 2004-04-13 2005-11-24 Quintessence Biosciences, Inc. Non-natural ribonuclease conjugates as cytotoxic agents
WO2006138558A2 (en) * 2005-06-16 2006-12-28 Wisconsin Alumni Research Foundation Cytotoxic ribonuclease variants
JP2010180174A (ja) * 2009-02-06 2010-08-19 Tokyo Univ Of Science 膵癌の診断方法および膵癌の診断用キット
WO2011161260A1 (en) * 2010-06-25 2011-12-29 Adamed Sp. Z O.O. Anticancer fusion protein

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE 0 "Human pancreas ribonucleasel (RNasel) sequence, SEQ ID I", accession no. YG98365 *
DATABASE 0 "Human pancreatic ribonucleasel (RNase 1) chain X , SEQ ID 2", accession no. ZS55938 *
DATABASE 0 "Human RNase 1 protein SEQID N° 4", accession no. DS52279 *
DATABASE ACCESSIONNUMBER accession no. EE02927 *
PERE TUBERT ET AL.: "The nuclear transport capacity of a human-pancreatic ribonuclease variant is critical for its cytotoxicity. Investigational New Drugs", THE JOURNAL OF NEW ANTICANCER AGENTS., vol. 29, no. 5, October 2011 (2011-10-01), pages 811 - 817 *

Also Published As

Publication number Publication date
ES2388020A1 (es) 2012-10-05
ES2388020B1 (es) 2013-10-01
WO2012120176A2 (es) 2012-09-13

Similar Documents

Publication Publication Date Title
WO2013151672A3 (en) Modified polynucleotides for the production of oncology-related proteins and peptides
WO2013055888A3 (en) Recombinant human naglu protein and uses thereof
MX348071B (es) Variantes de fc.
NZ705370A (en) Fcγriib-specific fc region variant
WO2013184939A3 (en) Fusion polypeptides comprising an active protein linked to a mucin-domain polypeptide
WO2013003641A3 (en) Serpin fusion polypeptides and methods of use thereof
WO2013075066A3 (en) Variant serum albumin with improved half -life and other properties
PH12015500528A1 (en) Fc containing polypeptides with altered glycosylation and reduced
EP4223772A3 (en) Optimized factor viii gene
MA34077B1 (fr) Nouveau peptides et procedes pour leur preparation, ain que leur utilisation
MX2015001675A (es) Proteinas de fusion de interleuquina 10 y usos de las mismas.
MX2014004214A (es) Moleculas de acido ribonucleico policistronicas auto-replicantes recombinantes.
MX351916B (es) Polipeptidos que tienen actividad de lisozima y polinucleotdos que los codifican.
WO2012054929A3 (en) Use of human serum albumin to decrease antigenicity of therapeutic proteins
NZ742660A (en) Variants of chymosin with improved milk-clotting properties
WO2012160563A3 (en) Use of akt phosphorylation as a biomarker for prognosing neurodegenerative diseases and treating same
NZ713602A (en) Method for refining protein including self-cutting cassette and use thereof
NZ629843A (en) Identification of channelrhodopsin-2 (chop2) mutations and methods of use
WO2012135177A3 (en) Methods and compositions for cytomegalovirus il-10 protein
IN2015DN03206A (es)
NZ760789A (en) Uti fusion proteins
HK1215441A1 (zh) 人血清白蛋白结合化合物及其融合蛋白
SG10201901695RA (en) Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers
MX2014011890A (es) Secuencias de acidos nucleicos y peptidos/proteinas de la familia de ft que proporcionan propiedades represoras de la floracion en plantas de tabaco y en plantas transgenicas transformadas con las mismas.
WO2012122378A3 (en) Antibody-like proteins for therapeutic and diagnostic use

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 12755442

Country of ref document: EP

Kind code of ref document: A2