WO2012124941A3 - Micro-organisme recombinant capable de produire un polyhydroxyalcanoate contenant du 2-hydroxybutyrate comme monomère, et procédé l'utilisant pour produire un polyhydroxyalcanoate contenant du 2-hydroxybutyrate comme monomère - Google Patents

Micro-organisme recombinant capable de produire un polyhydroxyalcanoate contenant du 2-hydroxybutyrate comme monomère, et procédé l'utilisant pour produire un polyhydroxyalcanoate contenant du 2-hydroxybutyrate comme monomère Download PDF

Info

Publication number
WO2012124941A3
WO2012124941A3 PCT/KR2012/001752 KR2012001752W WO2012124941A3 WO 2012124941 A3 WO2012124941 A3 WO 2012124941A3 KR 2012001752 W KR2012001752 W KR 2012001752W WO 2012124941 A3 WO2012124941 A3 WO 2012124941A3
Authority
WO
WIPO (PCT)
Prior art keywords
hydroxybutyrate
monomer
polyhydroxyalkanoate containing
recombinant microorganism
ability
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2012/001752
Other languages
English (en)
Korean (ko)
Other versions
WO2012124941A2 (fr
WO2012124941A9 (fr
Inventor
이상엽
박시재
이승환
송봉근
이태우
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Research Institute of Chemical Technology
Korea Advanced Institute of Science and Technology
Original Assignee
Korea Research Institute of Chemical Technology
Korea Advanced Institute of Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Research Institute of Chemical Technology, Korea Advanced Institute of Science and Technology filed Critical Korea Research Institute of Chemical Technology
Publication of WO2012124941A2 publication Critical patent/WO2012124941A2/fr
Publication of WO2012124941A3 publication Critical patent/WO2012124941A3/fr
Publication of WO2012124941A9 publication Critical patent/WO2012124941A9/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Biological Depolymerization Polymers (AREA)
  • Polyesters Or Polycarbonates (AREA)

Abstract

La présente invention concerne un polyhydroxyalcanoate contenant du 2-hydroxybutyrate comme monomère, un micro-organisme recombinant capable de produire le polyhydroxyalcanoate contenant du 2-hydroxybutyrate comme monomère et un procédé préparation du polyhydroxyalcanoate contenant du 2-hydroxybutyrate comme monomère. Le polyhydroxyalcanoate contenant du 2-hydroxybutyrate selon l'invention est un nouveau polymère biodégradable. La présente invention concerne également le micro-organisme recombinant permettant de le produire.
PCT/KR2012/001752 2011-03-11 2012-03-09 Micro-organisme recombinant capable de produire un polyhydroxyalcanoate contenant du 2-hydroxybutyrate comme monomère, et procédé l'utilisant pour produire un polyhydroxyalcanoate contenant du 2-hydroxybutyrate comme monomère Ceased WO2012124941A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0022021 2011-03-11
KR1020110022021A KR101328567B1 (ko) 2011-03-11 2011-03-11 ２­하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트를 생산하는 능력을 가지는 재조합 미생물 및 이를 이용한 ２­하이드록시부티레이트를 모노머로 함유하고 있는 폴리하이드록시알카노에이트의 제조방법

Publications (3)

Publication Number Publication Date
WO2012124941A2 WO2012124941A2 (fr) 2012-09-20
WO2012124941A3 true WO2012124941A3 (fr) 2012-12-27
WO2012124941A9 WO2012124941A9 (fr) 2013-02-21

Family

ID=46831182

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/001752 Ceased WO2012124941A2 (fr) 2011-03-11 2012-03-09 Micro-organisme recombinant capable de produire un polyhydroxyalcanoate contenant du 2-hydroxybutyrate comme monomère, et procédé l'utilisant pour produire un polyhydroxyalcanoate contenant du 2-hydroxybutyrate comme monomère

Country Status (2)

Country Link
KR (1) KR101328567B1 (fr)
WO (1) WO2012124941A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101630003B1 (ko) * 2011-11-28 2016-06-13 주식회사 엘지화학 2-하이드록시알카노에이트 중합체의 제조방법
KR102088502B1 (ko) * 2015-09-03 2020-03-12 주식회사 엘지화학 4-하이드록시부티레이트, 3-하이드록시부티레이트, 2-하이드록시부티레이트 및 락테이트를 반복단위로 포함하는 사중합체 및 이의 제조방법
KR102088503B1 (ko) * 2015-09-03 2020-03-12 주식회사 엘지화학 4-하이드록시부티레이트, 3-하이드록시부티레이트 및 2-하이드록시부티레이트를 반복단위로 포함하는 삼중합체 및 이의 제조방법
KR102088504B1 (ko) * 2015-09-03 2020-03-12 주식회사 엘지화학 3-하이드록시프로피오네이트, 2-하이드록시부티레이트 및 락테이트를 반복단위로 포함하는 삼중합체 및 이의 제조방법
KR102497785B1 (ko) * 2018-01-08 2023-02-07 주식회사 엘지화학 2-하이드록시부티레이트 및 락테이트의 공중합체 제조를 위한 재조합 균주 및 방법
KR102361618B1 (ko) * 2018-01-08 2022-02-09 주식회사 엘지화학 2-하이드록시부티레이트 및 3-하이드록시부티레이트의 공중합체 및 이의 제조방법
KR20190090602A (ko) * 2018-01-25 2019-08-02 주식회사 엘지화학 2-하이드록시부티레이트, 2-하이드록시이소발레레이트 및 락테이트의 삼중합체 제조를 위한 재조합 균주 및 방법
CN115851510B (zh) * 2022-10-13 2023-09-12 深圳中科翎碳生物科技有限公司 盐单胞菌及其在联产四氢嘧啶和聚羟基脂肪酸酯的应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080275208A1 (en) * 1998-05-22 2008-11-06 Metabolix, Inc. Delaware Polyhydroxyalkanoate biopolymer compositions
KR20090078925A (ko) * 2008-01-16 2009-07-21 주식회사 엘지화학 폴리락테이트 또는 그 공중합체 생성능을 가지는 재조합미생물 및 이를 이용한 폴리락테이트 또는 그 공중합체의제조방법
KR20100090019A (ko) * 2009-02-05 2010-08-13 주식회사 엘지화학 폴리락틱산 또는 폴리락틱산 공중합체 생성능을 지닌 재조합 랄스토니아 유트로파 및 이것을 이용하여 폴리락틱산 또는 폴리락틱산 공중합체를 제조하는 방법

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080275208A1 (en) * 1998-05-22 2008-11-06 Metabolix, Inc. Delaware Polyhydroxyalkanoate biopolymer compositions
KR20090078925A (ko) * 2008-01-16 2009-07-21 주식회사 엘지화학 폴리락테이트 또는 그 공중합체 생성능을 가지는 재조합미생물 및 이를 이용한 폴리락테이트 또는 그 공중합체의제조방법
KR20100090019A (ko) * 2009-02-05 2010-08-13 주식회사 엘지화학 폴리락틱산 또는 폴리락틱산 공중합체 생성능을 지닌 재조합 랄스토니아 유트로파 및 이것을 이용하여 폴리락틱산 또는 폴리락틱산 공중합체를 제조하는 방법

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
JIA JIAN ET AL.: "Production ofpolyhydroxyalkanoates by Escherichia coli mutants with defected mixed acid fermentation pathways", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 87, no. 6, August 2010 (2010-08-01), pages 2247 - 2256 *
KEN' ICHIRO MATSUMOTO ET AL.: "Enzymatic and whole-cell synthesis of lactate-containing polyesters: toward the complete biological production of polylactate", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 85, no. 4, January 2010 (2010-01-01), pages 921 - 932 *
TAEK HO YANG ET AL.: "Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase", BIOTECHNOLOGY AND BIOENGINEERING, vol. 105, no. 1, 1 January 2010 (2010-01-01), pages 150 - 160 *
YU KYUNG JUNG ET AL.: "Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers", BIOTECHNOLOGY AND BIOENGINEERING, vol. 105, no. 1, 1 January 2010 (2010-01-01), pages 161 - 171 *
YUYANG SONG ET AL.: "Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 93, no. 5, 30 November 2011 (2011-11-30), pages 1917 - 1925 *

Also Published As

Publication number Publication date
KR20120103997A (ko) 2012-09-20
WO2012124941A2 (fr) 2012-09-20
KR101328567B1 (ko) 2013-11-13
WO2012124941A9 (fr) 2013-02-21

Similar Documents

Publication Publication Date Title
WO2012169819A3 (fr) Procédé de préparation de polyhydroxyalcanoate contenant du 2-hydroxybutyrate en tant que monomère
PH12015501541A1 (en) Preparation of poly alpha-1, 3-glucan esters and films therefrom
EP2913302A4 (fr) Procédé de production d'halogénure de cyanogène, composé d'ester de cyanate et son procédé de production, et composition de résine
WO2012138801A3 (fr) Compositions de lignine, procédés de production des compositions, procédés d'utilisations desdites compositions de lignine et produits élaborés de la sorte
EP2612877A4 (fr) Procédé pour la production d'un copolymère à blocs de poly(acide lactique)
MY160881A (en) Process for the production of water and solvent-free polymers
WO2012014076A3 (fr) Organoïde hépatique, ses utilisations, et procédé de culture permettant de l'obtenir
WO2012124937A3 (fr) Fibre conjuguée à base de graphène et son procédé de fabrication
WO2012173659A3 (fr) Hydrolysats lignocellulosiques comme charges d'alimentation pour la fermentation de l'isobutanol
TN2013000347A1 (en) Production method of optically active dihydrobenzofuran derivative
WO2011073341A3 (fr) Dispersions de polymères
WO2013105802A3 (fr) Microorganismes de corynebacterium qui peuvent utiliser le xylose, et procédé de production de l-lysine l'utilisant
WO2013163230A3 (fr) Polymères d'origine biologique et leurs procédés de production
WO2014060099A3 (fr) Microsphères
WO2011089595A3 (fr) Compositions matricielles d'acide nucléique à libération prolongée
PH12013501775B1 (en) Polymeric acid catalysts and uses thereof
WO2011123854A3 (fr) Bioaugmentation des systèmes de digesteurs anaérobies
BR112012031895A2 (pt) processo para produzir as partículas poliméricas absorvedoras de água, partículas poliméricas absorvedoras de água, e, artigo de higiene
BR112014004644A2 (pt) polímeros funcionalizados com lactonas ou tiolactonas contendo um grupo amino protegido
WO2013059523A3 (fr) Matière carbonée utilisée pour purifier des oligomères lignocellulosiques
MX2015007952A (es) Proceso para la produccion de un metacrilato de alquilo.
WO2012146693A3 (fr) Garniture de gâteau au fromage pouvant être stockée à température ambiante
WO2014049382A3 (fr) Production de fermentation d'éthylènediamine par un micro-organisme recombinant
WO2012016172A3 (fr) Microbes génétiquement modifiés produisant de plus hauts niveaux de composés dérivés d'acétyl-coa
WO2012063115A3 (fr) Procédé de préparation de rosuvastatine calcique via un nouvel intermédiaire amine

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12758049

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 12758049

Country of ref document: EP

Kind code of ref document: A2