WO2012124946A3 - 재조합 대장균을 이용한 ５-아미노발레르산의 제조 방법 - Google Patents

재조합 대장균을 이용한 ５-아미노발레르산의 제조 방법 Download PDF

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Publication number
WO2012124946A3
WO2012124946A3 PCT/KR2012/001757 KR2012001757W WO2012124946A3 WO 2012124946 A3 WO2012124946 A3 WO 2012124946A3 KR 2012001757 W KR2012001757 W KR 2012001757W WO 2012124946 A3 WO2012124946 A3 WO 2012124946A3
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WIPO (PCT)
Prior art keywords
escherichia coli
aminovaleric acid
producing
recombinant escherichia
lysine
Prior art date
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Ceased
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PCT/KR2012/001757
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English (en)
French (fr)
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WO2012124946A2 (ko
Inventor
박시재
이승환
송봉근
제갈종건
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Korea Research Institute of Chemical Technology KRICT
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Korea Research Institute of Chemical Technology KRICT
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Publication of WO2012124946A2 publication Critical patent/WO2012124946A2/ko
Publication of WO2012124946A3 publication Critical patent/WO2012124946A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12002—Lysine 2-monooxygenase (1.13.12.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/0103—5-Aminopentanamidase (3.5.1.30)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 재조합 대장균 균주를 이용한 라이신(lysine)으로부터 5-아미노발레르산(5-aminovaleric acid)의 제조 방법에 관한 것으로, 보다 구체적으로 재조합 대장균 균주를 델타-아미노발레라미다아제(delta-aminovaleramidase; DavA) 및 라이신 2-단일산화효소(lysine 2-monooxygenase; DavB) 유전자로 형질전환하여 제조한 재조합 균주에 의해 라이신으로부터 5-아미노발레르산을 생산하는 방법에 관한 것이며, 상기 재조합 대장균 균주는 라이신으로부터 5-아미노발레르산으로의 전환 생산능이 있으므로, 상기 방법은 5-아미노발레르산 저가의 대량 생산기술에 유용하게 이용될 수 있다.
PCT/KR2012/001757 2011-03-11 2012-03-09 재조합 대장균을 이용한 ５-아미노발레르산의 제조 방법 Ceased WO2012124946A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110021963A KR101292838B1 (ko) 2011-03-11 2011-03-11 재조합 대장균을 이용한 ５―아미노발레르산의 제조 방법
KR10-2011-0021963 2011-03-11

Publications (2)

Publication Number Publication Date
WO2012124946A2 WO2012124946A2 (ko) 2012-09-20
WO2012124946A3 true WO2012124946A3 (ko) 2012-12-13

Family

ID=46831186

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Application Number Title Priority Date Filing Date
PCT/KR2012/001757 Ceased WO2012124946A2 (ko) 2011-03-11 2012-03-09 재조합 대장균을 이용한 ５-아미노발레르산의 제조 방법

Country Status (2)

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KR (1) KR101292838B1 (ko)
WO (1) WO2012124946A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102597723B1 (ko) 2021-10-20 2023-11-03 홍익대학교세종캠퍼스산학협력단 5-아미노발레르산으로부터 글루타르산으로의 전환 방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070134231A1 (en) * 2005-12-13 2007-06-14 Jani Dharmendra M Method for prolonging activity of autodegradable enzymes and compositions thereof
US20100168481A1 (en) * 2008-12-12 2010-07-01 Metabolix,Inc. Green process and compositions for producing poly(5hv) and 5 carbon chemicals

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070134231A1 (en) * 2005-12-13 2007-06-14 Jani Dharmendra M Method for prolonging activity of autodegradable enzymes and compositions thereof
US20100168481A1 (en) * 2008-12-12 2010-07-01 Metabolix,Inc. Green process and compositions for producing poly(5hv) and 5 carbon chemicals

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OLGA REVELLES ET AL.: "Multiple and Interconnected Pathways for L-Lysine Catabolism in Pseudomonas putida KT2440", J. BACTERIOL., November 2005 (2005-11-01), pages 7500 - 7510 *

Also Published As

Publication number Publication date
KR101292838B1 (ko) 2013-08-05
KR20120103959A (ko) 2012-09-20
WO2012124946A2 (ko) 2012-09-20

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