WO2012128577A3 - 패혈증 단백질 치료제의 효능 분석 및 스크리닝 방법 - Google Patents

패혈증 단백질 치료제의 효능 분석 및 스크리닝 방법 Download PDF

Info

Publication number
WO2012128577A3
WO2012128577A3 PCT/KR2012/002091 KR2012002091W WO2012128577A3 WO 2012128577 A3 WO2012128577 A3 WO 2012128577A3 KR 2012002091 W KR2012002091 W KR 2012002091W WO 2012128577 A3 WO2012128577 A3 WO 2012128577A3
Authority
WO
WIPO (PCT)
Prior art keywords
screening
septicaemia
therapeutic agents
performance analysis
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2012/002091
Other languages
English (en)
French (fr)
Other versions
WO2012128577A2 (ko
Inventor
이승구
김학성
김유정
이상철
한지은
김수진
손정훈
하재석
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Advanced Institute of Science and Technology KAIST
Korea Research Institute of Bioscience and Biotechnology KRIBB
Original Assignee
Korea Advanced Institute of Science and Technology KAIST
Korea Research Institute of Bioscience and Biotechnology KRIBB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Advanced Institute of Science and Technology KAIST, Korea Research Institute of Bioscience and Biotechnology KRIBB filed Critical Korea Advanced Institute of Science and Technology KAIST
Publication of WO2012128577A2 publication Critical patent/WO2012128577A2/ko
Publication of WO2012128577A3 publication Critical patent/WO2012128577A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/15—Medicinal preparations ; Physical properties thereof, e.g. dissolubility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional [2D] or three-dimensional [3D] molecular structures, e.g. structural or functional relations or structure alignment
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional [2D] or three-dimensional [3D] molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/20—Protein or domain folding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional [2D] or three-dimensional [3D] molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/30—Drug targeting using structural data; Docking or binding prediction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 LRR(Leucine rich repeat) 모듈의 반복적 조합에 의하여 구성된, LRR 단백질의 패혈증 저해 단백질 치료제로서의 효능을 분석하고, 서열변이 라이브러리로부터 우수한 저해효능을 보이는 단백질 치료제를 스크리닝하는 어세이 시스템의 구축에 관한 것이다. 보다 구체적으로, LPS 신호 전달 수용체가 표면에 발현된 세포주를 이용한 어세이 시스템에 정제한 MD-2 단백질을 첨가하는 단계를 포함하는 패혈증 단백질 치료제의 효능 분석 방법 또는 스크리닝 방법, THP-1 세포주에 정제한 외래 MD-2 단백질을 첨가하는 단계를 포함하는 패혈증 단백질 치료제의 효능 분석 방법 또는 스크리닝 방법, 상기 효능 분석용 또는 스크리닝용 세포주, 상기 세포주 및 정제한 외래 MD-2를 포함하는 패혈증 치료제 스크리닝용 또는 효능 분석용 키트에 관한 것이다.
PCT/KR2012/002091 2011-03-22 2012-03-22 패혈증 단백질 치료제의 효능 분석 및 스크리닝 방법 Ceased WO2012128577A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110025446A KR20120107741A (ko) 2011-03-22 2011-03-22 패혈증 단백질 치료제의 효능 분석 및 스크리닝 방법
KR10-2011-0025446 2011-03-22

Publications (2)

Publication Number Publication Date
WO2012128577A2 WO2012128577A2 (ko) 2012-09-27
WO2012128577A3 true WO2012128577A3 (ko) 2013-01-24

Family

ID=46879569

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/KR2012/002091 Ceased WO2012128577A2 (ko) 2011-03-22 2012-03-22 패혈증 단백질 치료제의 효능 분석 및 스크리닝 방법
PCT/KR2012/002094 Ceased WO2012128580A1 (en) 2011-03-22 2012-03-22 Water-soluble polypeptides comprised of repeat modules, method for preparing the same and method for a target-specific polypeptide and analysis of biological activity thereof

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/002094 Ceased WO2012128580A1 (en) 2011-03-22 2012-03-22 Water-soluble polypeptides comprised of repeat modules, method for preparing the same and method for a target-specific polypeptide and analysis of biological activity thereof

Country Status (3)

Country Link
US (1) US9441017B2 (ko)
KR (1) KR20120107741A (ko)
WO (2) WO2012128577A2 (ko)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6824594B2 (ja) 2014-09-11 2021-02-03 Jnc株式会社 合成遺伝子の設計方法
AU2015366212B2 (en) 2014-12-19 2021-04-29 Sanofi Pasteur Multimerization of recombinant protein by fusion to a sequence from lamprey
SE1500434A1 (en) * 2015-10-29 2017-04-30 Theravac Pharmaceuticals Ab A novel fusion partner for highly efficient and safe vaccines
AU2016359230B2 (en) 2015-11-25 2020-04-23 Ligachem Biosciences Inc. Conjugates comprising self-immolative groups and methods related thereto
KR102847348B1 (ko) 2015-11-25 2025-08-19 주식회사 리가켐바이오사이언스 펩타이드 그룹을 포함하는 접합체 및 이와 관련된 제조방법
AU2016359235B2 (en) 2015-11-25 2022-09-15 Ligachem Biosciences Inc. Antibody-drug conjugates comprising branched linkers and methods related thereto
KR101840364B1 (ko) * 2016-04-25 2018-03-20 주식회사 엔지노믹스 Taq DNA 중합효소에 특이적으로 결합하는 폴리펩타이드 및 그 용도
CN106148286B (zh) * 2016-06-29 2019-10-29 牛刚 一种用于检测热原的细胞模型的构建方法和细胞模型及热原检测试剂盒
KR101955928B1 (ko) 2016-12-30 2019-03-08 경상대학교산학협력단 먹장어 유래 vlrb 단백질의 소수성 테일 도메인을 포함하는 다량체 다가 중합체
CA3058360A1 (en) 2017-03-29 2018-10-04 Legochem Biosciences, Inc. Pyrrolobenzodiazepine dimer prodrug and ligand-linker conjugate compound of the same
KR101972899B1 (ko) * 2017-04-24 2019-04-26 경상대학교산학협력단 소수성 테일 도메인이 제거된 먹장어 유래 VLRB 단백질과 C4bp 올리고머화 도메인이 연결된 융합 단백질 및 이의 용도
CN112601555A (zh) 2018-05-09 2021-04-02 乐高化学生物科学股份有限公司 与抗cd19抗体药物结合物相关的组合物和方法
KR102231974B1 (ko) * 2019-08-23 2021-03-25 한국과학기술원 사람의 프로그램된 세포사멸 단백질(pd-1)에 선택적으로 결합하는 신규한 폴리펩티드 및 이의 용도
KR20210028544A (ko) 2019-09-04 2021-03-12 주식회사 레고켐 바이오사이언스 인간 ror1에 대한 항체를 포함하는 항체 약물 접합체 및 이의 용도
KR102280725B1 (ko) * 2019-10-02 2021-07-23 한국과학기술원 인간 b 세포 림프종-2 단백질에 선택적으로 결합하는 신규한 폴리펩타이드 및 이의 용도
KR102690857B1 (ko) * 2021-04-30 2024-08-05 한국과학기술원 제2형 중증급성호흡기증후군 코로나바이러스의 수용체 결합 도메인 (SARS-CoV2 RBD)에 선택적으로 결합하는 신규한 폴리펩티드 및 이의 용도
CN114596912B (zh) * 2022-02-18 2023-08-29 五邑大学 一种基于多肽长度的短肽组学鉴定方法及其应用
CN118910106A (zh) * 2023-05-08 2024-11-08 上海岱翱生物科技有限公司 一种tshr融合蛋白及其制备方法和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1657257A1 (en) * 2004-11-11 2006-05-17 Klinikum der Universität Regensburg Recombinant TLR-MD-2 fusion proteins

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2138508A1 (en) * 2008-06-26 2009-12-30 Helmholtz-Zentrum für Infektionsforschung GmbH Met agonists
KR101219628B1 (ko) 2010-03-02 2013-01-10 한국과학기술원 인터날린 단백질의 ｎ-말단 및 ｌｒｒ 패밀리 단백질이 융합된 수용성 융합 폴리펩타이드 및 이의 제조 방법

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1657257A1 (en) * 2004-11-11 2006-05-17 Klinikum der Universität Regensburg Recombinant TLR-MD-2 fusion proteins

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
JUNG, K. ET AL.: "Toll-like receptor 4 decoy, TOY, attenates gram-negative bacterial sepsis", PLOS ONE, vol. 4, no. ISS.10, 9 October 2009 (2009-10-09), pages E7403 *
MATSUNAGA, N. ET AL.: "TAK242(Reasatorvid), a small-molecule inhibitor of Toll-like receptor(TLR) 4 signaling, binds selectively to TLR4 an interferes with interactions between TLR4 and its adaptor molecules", MOLECULAR PHARMACOLOGY, vol. 79, no. 1, January 2001 (2001-01-01), pages 34 - 41, XP055053425, DOI: doi:10.1124/mol.110.068064 *
PUGIN, J ET AL.: "Soluble MD-2 activity in plasma from patients with severe sepsis and septic shock", BLOOD, vol. 104, 24 August 2004 (2004-08-24), pages 4071 - 4079 *
WEISS, T. ET AL.: "Anti-inflammatory effects of moxifloxacin on activated human monocytic cell: inhibition of NF-kB and mitogen-activated protein kinase activation and of synthesis of proinflammatory cytokines", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 48, no. 6, June 2004 (2004-06-01), pages 1974 - 1982, XP003032904, DOI: doi:10.1128/AAC.48.6.1974-1982.2004 *

Also Published As

Publication number Publication date
WO2012128580A1 (en) 2012-09-27
US20140088292A1 (en) 2014-03-27
KR20120107741A (ko) 2012-10-04
US9441017B2 (en) 2016-09-13
WO2012128577A2 (ko) 2012-09-27

Similar Documents

Publication Publication Date Title
WO2012128577A3 (ko) 패혈증 단백질 치료제의 효능 분석 및 스크리닝 방법
EA201390039A1 (ru) Способ разделения газов
EA201171148A1 (ru) Антитело-антагонист, специфичное для гетеродимера альфа-4-бета-7
WO2012154794A3 (en) Enzyme concentration and assays
EA201100300A1 (ru) Композиции и способы, предназначенные для направленного воздействия антител на белок c5 системы комплемента
HK1206755A1 (en) Low acidic species compositions and methods for producing and using the same
WO2013138793A3 (en) Affinity reagants for protein purification
WO2013006449A3 (en) Anti-properdin antibodies and uses thereof
WO2010132453A3 (en) Methods and compositions for analyte detection
CA2864035C (en) A method for determining the presence of an analyte using an aptamer
WO2011136624A3 (ko) 자기장 인가부를 구비한 생물학적 시료 자동정제장치, 생물학적 시료로부터 타겟물질을 추출하는 방법, 그리고 단백질 발현 및 정제 방법
BR112014012275A2 (pt) purificação de proteína com a utilização de tampão bis-tris
WO2013036929A8 (en) Methods for obtaining a sequence
WO2011156529A3 (en) Methods and composition for multiplex sequencing
EP4361607A3 (en) Signal encoding and decoding in multiplexed biochemical assays
EA201101593A1 (ru) КОМПОЗИЦИИ И СПОСОБЫ, ПРЕДНАЗНАЧЕННЫЕ ДЛЯ НАПРАВЛЕННОГО ВОЗДЕЙСТВИЯ АНТИТЕЛ НА БЕЛОК С3b СИСТЕМЫ КОМПЛЕМЕНТА
MX372998B (es) Proteinas de captura de superficie celular recombinantes.
WO2010133963A3 (en) Forming sample combinations using liquid bridge systems
WO2009083950A3 (en) Biomarkers for the prediction of renal injury
WO2011121454A3 (en) Direct nucleic acid analysis
WO2011109326A3 (en) Crosslinking reagents, methods, and compositions for studying protein-protein interactions
WO2010115745A3 (en) Biomarkers, methods and kits for the diagnosis of rheumatoid arthritis
WO2011120690A3 (en) Separation of insoluble target proteins
EP2133342A4 (en) INHIBITOR OF PROTEINTHYROSINE PHOSPHATASE 1B, METHOD OF MANUFACTURE AND APPLICATIONS THEREOF
WO2010039802A3 (en) Methods and compositions for isolating nucleic acid

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12760751

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 12760751

Country of ref document: EP

Kind code of ref document: A2