WO2012158982A3 - Méthodes pour effectuer une transgenèse d'organismes et de cellules souches spermatogoniales (ssc) à l'aide de vecteurs de transposons - Google Patents

Méthodes pour effectuer une transgenèse d'organismes et de cellules souches spermatogoniales (ssc) à l'aide de vecteurs de transposons Download PDF

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Publication number
WO2012158982A3
WO2012158982A3 PCT/US2012/038461 US2012038461W WO2012158982A3 WO 2012158982 A3 WO2012158982 A3 WO 2012158982A3 US 2012038461 W US2012038461 W US 2012038461W WO 2012158982 A3 WO2012158982 A3 WO 2012158982A3
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WO
WIPO (PCT)
Prior art keywords
sscs
methods
transgenesis
stem cells
organisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/038461
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English (en)
Other versions
WO2012158982A2 (fr
Inventor
Eric M. Ostertag
John S. CRAWFORD
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transposagen Biopharmaceuticals Inc
Original Assignee
Transposagen Biopharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transposagen Biopharmaceuticals Inc filed Critical Transposagen Biopharmaceuticals Inc
Publication of WO2012158982A2 publication Critical patent/WO2012158982A2/fr
Publication of WO2012158982A3 publication Critical patent/WO2012158982A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/03—Animals modified by random mutagenesis, e.g. using ENU, chemicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/99—Coculture with; Conditioned medium produced by genetically modified cells

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes de transgenèse reposant sur des transposons, dans des cellules souches spermatogoniales (SSC) de plusieurs espèces de mammifères.
PCT/US2012/038461 2011-05-17 2012-05-17 Méthodes pour effectuer une transgenèse d'organismes et de cellules souches spermatogoniales (ssc) à l'aide de vecteurs de transposons Ceased WO2012158982A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161487274P 2011-05-17 2011-05-17
US61/487,274 2011-05-17

Publications (2)

Publication Number Publication Date
WO2012158982A2 WO2012158982A2 (fr) 2012-11-22
WO2012158982A3 true WO2012158982A3 (fr) 2013-03-14

Family

ID=47177644

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/038461 Ceased WO2012158982A2 (fr) 2011-05-17 2012-05-17 Méthodes pour effectuer une transgenèse d'organismes et de cellules souches spermatogoniales (ssc) à l'aide de vecteurs de transposons

Country Status (1)

Country Link
WO (1) WO2012158982A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105238818A (zh) * 2015-09-29 2016-01-13 潘雨堃 一种在体内精原干细胞基因组中引入随机插入突变的方法
CN108378019B (zh) * 2018-03-13 2021-03-02 诺赛联合(北京)生物医学科技有限公司 一种人精原干细胞的冻存液
CN111850037A (zh) * 2019-04-30 2020-10-30 潘雨堃 一种piggyBac转座子高通量遗传筛选方法
CN116004717A (zh) * 2022-11-11 2023-04-25 北京希诺因生物科技有限公司 Als疾病模型犬的建立方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060288438A1 (en) * 1999-07-21 2006-12-21 Yoshihiro Ozeki Novel miniature inverted-repeat transposable elements (MITEs)-like element and transcriptional activation element
US20080279838A1 (en) * 2005-04-08 2008-11-13 Csaba Miskey Reconstructed Human Mariner Transposon Capable of Stable Gene Transfer Into Chromosomes in Vertebrates
WO2010065550A2 (fr) * 2008-12-01 2010-06-10 Board Of Regents University Of Texas System Production et utilisation de lignées de cellules souches spermatogoniales de rat
US20100287628A1 (en) * 2009-04-23 2010-11-11 Ostertag Eric M Genetically Modified Rat Models for Cancer

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060288438A1 (en) * 1999-07-21 2006-12-21 Yoshihiro Ozeki Novel miniature inverted-repeat transposable elements (MITEs)-like element and transcriptional activation element
US20080279838A1 (en) * 2005-04-08 2008-11-13 Csaba Miskey Reconstructed Human Mariner Transposon Capable of Stable Gene Transfer Into Chromosomes in Vertebrates
WO2010065550A2 (fr) * 2008-12-01 2010-06-10 Board Of Regents University Of Texas System Production et utilisation de lignées de cellules souches spermatogoniales de rat
US20100287628A1 (en) * 2009-04-23 2010-11-11 Ostertag Eric M Genetically Modified Rat Models for Cancer

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FENG ET AL.: "Precise targeted integration by a chimaeric transposase zinc-finger fusion protein.", NUCLEIC ACIDS RESEARCH, vol. 38, no. 4, March 2010 (2010-03-01), pages 1204 - 1216 *

Also Published As

Publication number Publication date
WO2012158982A2 (fr) 2012-11-22

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