WO2012170782A3 - Production de protéines recombinantes hydroxylées post-traductionnellement dans des bactéries - Google Patents

Production de protéines recombinantes hydroxylées post-traductionnellement dans des bactéries Download PDF

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Publication number
WO2012170782A3
WO2012170782A3 PCT/US2012/041497 US2012041497W WO2012170782A3 WO 2012170782 A3 WO2012170782 A3 WO 2012170782A3 US 2012041497 W US2012041497 W US 2012041497W WO 2012170782 A3 WO2012170782 A3 WO 2012170782A3
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WO
WIPO (PCT)
Prior art keywords
recombinant proteins
post
bacteria
production
translationally
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/041497
Other languages
English (en)
Other versions
WO2012170782A2 (fr
Inventor
Daniel M. Pinkas
Sheng Ding
Annelise E. Barron
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Leland Stanford Junior University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of WO2012170782A2 publication Critical patent/WO2012170782A2/fr
Publication of WO2012170782A3 publication Critical patent/WO2012170782A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne des cellules bactériennes aptes à produire des protéines recombinantes, telles que des protéines recombinantes hydroxylées post-traductionnellement, des procédés et des trousses pour la production de protéines recombinantes, telles que des protéines recombinantes hydroxylées post-traductionnellement et des molécules particulières de collagène recombinant hydroxylé post-traductionnellement, et une molécule comprenant un domaine collagène produite par les procédés et cellules décrits présentement.
PCT/US2012/041497 2011-06-10 2012-06-08 Production de protéines recombinantes hydroxylées post-traductionnellement dans des bactéries Ceased WO2012170782A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161495668P 2011-06-10 2011-06-10
US61/495,668 2011-06-10

Publications (2)

Publication Number Publication Date
WO2012170782A2 WO2012170782A2 (fr) 2012-12-13
WO2012170782A3 true WO2012170782A3 (fr) 2013-03-14

Family

ID=46458594

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/041497 Ceased WO2012170782A2 (fr) 2011-06-10 2012-06-08 Production de protéines recombinantes hydroxylées post-traductionnellement dans des bactéries

Country Status (1)

Country Link
WO (1) WO2012170782A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11478504B2 (en) 2013-07-08 2022-10-25 International Dehydrated Foods, Inc. Compositions and methods for preventing/treating metabolic syndrome
US11028146B2 (en) 2017-09-22 2021-06-08 Modern Meadow, Inc. Recombinant yeast strains
CA3073750A1 (fr) * 2017-09-28 2019-04-04 International Dehydrated Foods, Inc. Compositions et methodes de prevention/traitement du syndrome metabolique
CN111087464B (zh) * 2019-12-28 2021-10-29 河北纳科生物科技有限公司 一种具有功能结构的重组人源iii型胶原蛋白及其表达方法
CN115478055A (zh) * 2021-06-15 2022-12-16 佛山汉腾生物科技有限公司 一种调节重组蛋白羟基化水平的方法
CN119505021B (zh) * 2025-01-20 2025-04-18 内蒙古和讯生物科技有限公司 一种重复结构单元重组蛋白cpE2及其制备方法和应用

Family Cites Families (6)

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US5219576A (en) 1988-06-30 1993-06-15 Collagen Corporation Collagen wound healing matrices and process for their production
US20020098578A1 (en) 1992-10-22 2002-07-25 Darwin J. Prockop Synthesis of human procollagens and collagens in recombinant dna systems
US5800811A (en) 1995-06-06 1998-09-01 Hall; Frederick L. Artificial skin prepared from coclagen matrix containing transforming growth factor-β having a collagen binding site
US5928922A (en) 1996-04-10 1999-07-27 Academy Of Finland α2 subunit of prolyl-4-hydroxylase, nucleic acid sequences encoding such subunit and methods for producing the same
US7008397B2 (en) 2002-02-13 2006-03-07 Percardia, Inc. Cardiac implant and methods
US20050217034A1 (en) 2004-04-01 2005-10-06 Henkel Consumer Adhesives, Inc. Spackling kit and tool

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DO CARMO AVIDES ET AL: "Differentiation of human pre-adipocytes by recombinant adiponectin", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 59, no. 1, 26 January 2008 (2008-01-26), pages 122 - 126, XP022561026, ISSN: 1046-5928, DOI: 10.1016/J.PEP.2008.01.012 *
PINKAS DANIEL M ET AL: "Tunable, Post-translational Hydroxylation of Collagen Domains in Escherichia coli", ACS CHEMICAL BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 6, no. 4, 1 April 2011 (2011-04-01), pages 320 - 324, XP002648321, ISSN: 1554-8929 *
RICHARDS A A ET AL: "ADIPONECTIN MULTIMERIZATION IS DEPENDENT ON CONSERVED LYSINES IN THE COLLAGENOUS DOMAIN: EVIDENCE FOR REGULATION OF MULTIMERIZATION BY ALTERATIONS IN POSTTRANSLATIONAL MODIFICATIONS", MOLECULAR ENDOCRINOLOGY, THE ENDOCRINE SOCIETY, US, vol. 20, no. 7, 1 July 2006 (2006-07-01), pages 1673 - 1687, XP009071842, ISSN: 0888-8809, DOI: 10.1210/ME.2005-0390 *
SHENG DING ET AL: "Synthesis and Assembly of Functional High Molecular Weight Adiponectin Multimers in an Engineered Strain of Escherichia coli", BIOMACROMOLECULES, vol. 13, no. 4, 9 April 2012 (2012-04-09), pages 1035 - 1042, XP055037523, ISSN: 1525-7797, DOI: 10.1021/bm2017367 *

Also Published As

Publication number Publication date
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