WO2013088098A3 - Analyse efficace d'adn ultra-long dans des canaux nanofluidiques compacts - Google Patents

Analyse efficace d'adn ultra-long dans des canaux nanofluidiques compacts Download PDF

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Publication number
WO2013088098A3
WO2013088098A3 PCT/GB2012/000566 GB2012000566W WO2013088098A3 WO 2013088098 A3 WO2013088098 A3 WO 2013088098A3 GB 2012000566 W GB2012000566 W GB 2012000566W WO 2013088098 A3 WO2013088098 A3 WO 2013088098A3
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WO
WIPO (PCT)
Prior art keywords
compact
dna analysis
long dna
nanofluidic channels
efficient ultra
Prior art date
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Ceased
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PCT/GB2012/000566
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English (en)
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WO2013088098A2 (fr
Inventor
Kalim Mir
Jonas Tegenfeldt
Fredrick PERSSON
Joachim FRITZSCHE
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Publication of WO2013088098A2 publication Critical patent/WO2013088098A2/fr
Publication of WO2013088098A3 publication Critical patent/WO2013088098A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0896—Nanoscaled
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Nanotechnology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)

Abstract

La présente demande concerne des nanopuces, qui peuvent être utilisées pour visualiser de longues molécules dans un seul champ de vue, et les procédés d'utilisation des nanopuces. Ces procédés incluent la cartographie/codification à barres et le séquençage d'ADN.
PCT/GB2012/000566 2011-06-30 2012-07-02 Analyse efficace d'adn ultra-long dans des canaux nanofluidiques compacts Ceased WO2013088098A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1111237.2 2011-06-30
GB201111237A GB201111237D0 (en) 2011-06-30 2011-06-30 Nanochip

Publications (2)

Publication Number Publication Date
WO2013088098A2 WO2013088098A2 (fr) 2013-06-20
WO2013088098A3 true WO2013088098A3 (fr) 2013-10-17

Family

ID=44511940

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2012/000566 Ceased WO2013088098A2 (fr) 2011-06-30 2012-07-02 Analyse efficace d'adn ultra-long dans des canaux nanofluidiques compacts

Country Status (2)

Country Link
GB (1) GB201111237D0 (fr)
WO (1) WO2013088098A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014525921A (ja) 2011-08-05 2014-10-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸ナノテクノロジー及びマイクロテクノロジーに関する組成物及び方法
WO2013039778A2 (fr) 2011-09-12 2013-03-21 The University Of North Carolina At Chapel Hill Dispositifs comprenant un nanocanal de transport de fluide entrecoupé par un nanocanal de détection de fluide et procédés correspondants
JP6339024B2 (ja) 2012-02-10 2018-06-06 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒルＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｎｏｒｔｈ Ｃａｒｏｌｉｎａ Ａｔ Ｃｈａｐｅｌ Ｈｉｌｌ 流体ナノファンネルを有する装置、関連する方法、製造及び解析システム
WO2014018675A1 (fr) 2012-07-24 2014-01-30 President And Fellows Of Harvard College Auto-assemblage de nanostructures d'acide nucléique
WO2014074597A1 (fr) 2012-11-06 2014-05-15 President And Fellows Of Harvard College Compositions et procédés se rapportant à des nanostructures d'acides nucléiques complexes
KR20150132125A (ko) 2013-02-28 2015-11-25 더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐 거대분자의 통제된 포획, 고정, 및 전달을 위한 통합된 부품을 가진 나노유체 장치 및 관련 분석 방법
US9255288B2 (en) 2013-03-13 2016-02-09 The University Of North Carolina At Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US10190161B2 (en) 2014-04-03 2019-01-29 Stmicroelectronics S.R.L. Apparatus and method for nucleic acid sequencing based on nanowire detectors
US9765326B2 (en) 2014-04-03 2017-09-19 Stmicroelectronics S.R.L. Apparatus and method for nucleic acid sequencing based on nanochannels
JP2017523594A (ja) * 2014-05-22 2017-08-17 プレジデント アンド フェローズ オブ ハーバード カレッジ スケーラブルな核酸をベースにしたナノファブリケーション
CN112816679B (zh) * 2015-02-05 2024-05-28 哈佛大学校长及研究员协会 一种用于感测分子穿过纳米孔的移位的方法
US10471428B2 (en) 2015-05-11 2019-11-12 The University Of North Carolina At Chapel Hill Fluidic devices with nanoscale manifolds for molecular transport, related systems and methods of analysis
US10722888B2 (en) 2015-07-16 2020-07-28 The Hong Kong University Of Science And Technology Dynamic formation of nanochannels for single-molecule DNA analysis
CN115997030A (zh) * 2020-04-30 2023-04-21 德迈逊科技有限公司 用于大分子操作的装置和方法
CN116171331A (zh) * 2020-06-01 2023-05-26 德迈逊科技有限公司 用于基因组分析的装置和方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998035012A2 (fr) * 1997-02-12 1998-08-13 Chan Eugene Y Procedes et produits permettant d'analyser des polymeres
WO2001013088A1 (fr) * 1999-08-13 2001-02-22 U.S. Genomics, Inc. Procede et appareil d'etirage de polymeres
US20080273918A1 (en) * 2007-05-04 2008-11-06 Claros Diagnostics, Inc. Fluidic connectors and microfluidic systems
US20090115094A1 (en) * 2007-05-29 2009-05-07 Chou Stephen Y Methods for making continuous nanochannels
WO2009113010A1 (fr) * 2008-03-13 2009-09-17 Nxp B.V. Dispositif de capteur et procédé de détection de composés, de particules ou de complexes
WO2009149362A2 (fr) * 2008-06-06 2009-12-10 Bionanomatrix, Inc. Dispositifs d’analyse intégrés et procédés de fabrication associés et techniques d’analyse
WO2010042007A1 (fr) * 2008-10-10 2010-04-15 Jonas Tegenfeldt Procédé de cartographie du rapport at/gc local sur la longueur d'un fragment d'adn

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998035012A2 (fr) * 1997-02-12 1998-08-13 Chan Eugene Y Procedes et produits permettant d'analyser des polymeres
WO2001013088A1 (fr) * 1999-08-13 2001-02-22 U.S. Genomics, Inc. Procede et appareil d'etirage de polymeres
US20080273918A1 (en) * 2007-05-04 2008-11-06 Claros Diagnostics, Inc. Fluidic connectors and microfluidic systems
US20090115094A1 (en) * 2007-05-29 2009-05-07 Chou Stephen Y Methods for making continuous nanochannels
WO2009113010A1 (fr) * 2008-03-13 2009-09-17 Nxp B.V. Dispositif de capteur et procédé de détection de composés, de particules ou de complexes
WO2009149362A2 (fr) * 2008-06-06 2009-12-10 Bionanomatrix, Inc. Dispositifs d’analyse intégrés et procédés de fabrication associés et techniques d’analyse
WO2010042007A1 (fr) * 2008-10-10 2010-04-15 Jonas Tegenfeldt Procédé de cartographie du rapport at/gc local sur la longueur d'un fragment d'adn

Also Published As

Publication number Publication date
WO2013088098A2 (fr) 2013-06-20
GB201111237D0 (en) 2011-08-17

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