WO2013103246A3 - 퀴놀린산을 생산하는 재조합 미생물 및 이를 이용한 퀴놀린산의 생산 방법 - Google Patents

퀴놀린산을 생산하는 재조합 미생물 및 이를 이용한 퀴놀린산의 생산 방법 Download PDF

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Publication number
WO2013103246A3
WO2013103246A3 PCT/KR2013/000030 KR2013000030W WO2013103246A3 WO 2013103246 A3 WO2013103246 A3 WO 2013103246A3 KR 2013000030 W KR2013000030 W KR 2013000030W WO 2013103246 A3 WO2013103246 A3 WO 2013103246A3
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WO
WIPO (PCT)
Prior art keywords
quinolinic acid
same
recombinant microorganism
production method
microorganism producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2013/000030
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English (en)
French (fr)
Other versions
WO2013103246A2 (ko
Inventor
신용욱
김소영
허인경
김주은
손성광
이재희
이지현
김창겸
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CJ CheilJedang Corp
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CJ CheilJedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by CJ CheilJedang Corp filed Critical CJ CheilJedang Corp
Priority to ES13733832.3T priority Critical patent/ES2656892T3/es
Priority to CN201380009522.1A priority patent/CN104160015B/zh
Priority to US14/370,721 priority patent/US9534240B2/en
Priority to EP13733832.3A priority patent/EP2801612B1/en
Priority to BR112014016748-6A priority patent/BR112014016748B1/pt
Publication of WO2013103246A2 publication Critical patent/WO2013103246A2/ko
Publication of WO2013103246A3 publication Critical patent/WO2013103246A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K37/00—Special means in or on valves or other cut-off apparatus for indicating or recording operation thereof, or for enabling an alarm to be given
    • F16K37/0025—Electrical or magnetic means
    • F16K37/0041—Electrical or magnetic means for measuring valve parameters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C12P17/12—Nitrogen as only ring hetero atom containing a six-membered hetero ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/03—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12Y104/03016—L-Aspartate oxidase (1.4.3.16)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01072—Quinolinate synthase (2.5.1.72)
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K37/00—Special means in or on valves or other cut-off apparatus for indicating or recording operation thereof, or for enabling an alarm to be given
    • F16K37/0066—Hydraulic or pneumatic means
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 L-아스파르트산 산화효소 및 퀴놀린산 신타아제가 링커를 통하여 연결된 융합단백질을 발현하는 퀴놀린산을 생산하는 재조합 미생물 및 이를 이용하여 퀴놀린산을 생산하는 방법에 관한 것이다.
PCT/KR2013/000030 2012-01-06 2013-01-03 퀴놀린산을 생산하는 재조합 미생물 및 이를 이용한 퀴놀린산의 생산 방법 Ceased WO2013103246A2 (ko)

Priority Applications (5)

Application Number Priority Date Filing Date Title
ES13733832.3T ES2656892T3 (es) 2012-01-06 2013-01-03 Microorganismo recombinante que produce ácido quinolínico y procedimiento de producción de ácido quinolínico mediante su uso
CN201380009522.1A CN104160015B (zh) 2012-01-06 2013-01-03 生产喹啉酸的重组微生物及利用该微生物的喹啉酸生产方法
US14/370,721 US9534240B2 (en) 2012-01-06 2013-01-03 Recombinant microorganism producing quinolinic acid and a method for producing quinolinic acid using the same
EP13733832.3A EP2801612B1 (en) 2012-01-06 2013-01-03 Recombinant microorganism producing quinolinic acid and production method of quinolinic acid using same
BR112014016748-6A BR112014016748B1 (pt) 2012-01-06 2013-01-03 Microrganismo recombinante produtor de ácido quinolínico e um método para a produção de ácido quinolínico utilizando o mesmo

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020120001845A KR101359844B1 (ko) 2012-01-06 2012-01-06 퀴놀린산을 생산하는 재조합 미생물 및 이를 이용한 퀴놀린산의 생산 방법
KR10-2012-0001845 2012-01-06

Publications (2)

Publication Number Publication Date
WO2013103246A2 WO2013103246A2 (ko) 2013-07-11
WO2013103246A3 true WO2013103246A3 (ko) 2013-09-26

Family

ID=48745513

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2013/000030 Ceased WO2013103246A2 (ko) 2012-01-06 2013-01-03 퀴놀린산을 생산하는 재조합 미생물 및 이를 이용한 퀴놀린산의 생산 방법

Country Status (8)

Country Link
US (1) US9534240B2 (ko)
EP (1) EP2801612B1 (ko)
KR (1) KR101359844B1 (ko)
CN (1) CN104160015B (ko)
BR (1) BR112014016748B1 (ko)
ES (1) ES2656892T3 (ko)
MY (1) MY171877A (ko)
WO (1) WO2013103246A2 (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101634582B1 (ko) 2013-07-25 2016-06-30 씨제이제일제당 주식회사 니코틴산 또는 ｎａｄ에 대한 피드백 조절이 해제된 ｌ-아스파르테이트 옥시다제 변이체 및 이를 이용한 퀴놀리네이트 또는 니코틴산 생산 방법
KR101656363B1 (ko) * 2014-07-07 2016-09-09 씨제이제일제당 주식회사 퀴놀린산을 생산하는 재조합 미생물 및 이를 이용한 퀴놀린산의 생산 방법
KR101758446B1 (ko) * 2015-02-03 2017-07-17 씨제이제일제당 (주) 퀴놀린산 생산능을 가지는 미생물 및 이를 이용하여 퀴놀린산을 생산하는 방법
EA201892060A1 (ru) * 2016-03-16 2019-02-28 ДСМ АйПи АССЕТС Б.В. Микробная продукция рибозида никотиновой кислоты
CN106636024A (zh) * 2016-09-30 2017-05-10 苏州埃德蒙生物技术有限公司 一种l‑天冬氨酸氧化酶包涵体纯化复性方法
CN110982833B (zh) * 2019-12-25 2021-09-28 江南大学 一种对香豆酸响应的动态调控系统及其构建方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB332660A (en) * 1926-11-06 1930-07-31 Moritz Kugel Method of producing and maintaining constant the necessary supply of phosphoric acidin the electrolyte of lead accumulators
DE3826041C2 (ko) * 1988-07-30 1993-04-29 Ruetgerswerke Ag, 6000 Frankfurt, De
EP0279273B1 (de) * 1987-02-04 1993-12-15 Rütgerswerke Aktiengesellschaft DNA-Sequenzen, Plasmide und Mikroorganismen sowie Verfahren zur Herstellung von Chinolinsäure
US5664321A (en) * 1995-04-08 1997-09-09 Compagnie Europeenne D'accumulateurs Process for the production of a lead accumulator
US6271031B1 (en) * 1998-08-12 2001-08-07 E.I. Du Pont De Nemours And Company Quinolinate metabolism enzymes
JP2006172937A (ja) * 2004-12-16 2006-06-29 Zaisei Tomoda 鉛蓄電池用固体電解質およびその製造方法、鉛蓄電池
US20070202414A1 (en) * 2006-02-24 2007-08-30 Ngk Insulators, Ltd. All-solid-state battery

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SI2351772T1 (sl) * 2005-02-18 2016-11-30 Glaxosmithkline Biologicals Sa Proteini in nukleinske kisline iz Escherichia coli povezane z meningitisom/sepso
KR101223904B1 (ko) * 2011-01-14 2013-01-21 씨제이제일제당 (주) 니코틴산의 제조 방법

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB332660A (en) * 1926-11-06 1930-07-31 Moritz Kugel Method of producing and maintaining constant the necessary supply of phosphoric acidin the electrolyte of lead accumulators
EP0279273B1 (de) * 1987-02-04 1993-12-15 Rütgerswerke Aktiengesellschaft DNA-Sequenzen, Plasmide und Mikroorganismen sowie Verfahren zur Herstellung von Chinolinsäure
DE3826041C2 (ko) * 1988-07-30 1993-04-29 Ruetgerswerke Ag, 6000 Frankfurt, De
US5664321A (en) * 1995-04-08 1997-09-09 Compagnie Europeenne D'accumulateurs Process for the production of a lead accumulator
US6271031B1 (en) * 1998-08-12 2001-08-07 E.I. Du Pont De Nemours And Company Quinolinate metabolism enzymes
JP2006172937A (ja) * 2004-12-16 2006-06-29 Zaisei Tomoda 鉛蓄電池用固体電解質およびその製造方法、鉛蓄電池
US20070202414A1 (en) * 2006-02-24 2007-08-30 Ngk Insulators, Ltd. All-solid-state battery

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHANDLER, J. L. ET AL.: "De Novo Biosynthesis of Nicotinamide Adenine Dinucleotide in Escherichia coli: Excretion of Quinolinic Acid by Mutants Lacking Quinolinate Phosphoribosyl Transferase.", JOURNAL OF BACTERIOLOGY, vol. 111, no. 1, July 1972 (1972-07-01), pages 98 - 102, XP055113177 *

Also Published As

Publication number Publication date
BR112014016748A2 (pt) 2020-11-17
US9534240B2 (en) 2017-01-03
CN104160015A (zh) 2014-11-19
EP2801612A2 (en) 2014-11-12
BR112014016748B1 (pt) 2022-04-19
KR101359844B1 (ko) 2014-02-25
MY171877A (en) 2019-11-05
CN104160015B (zh) 2018-01-05
EP2801612A4 (en) 2015-07-29
WO2013103246A2 (ko) 2013-07-11
ES2656892T3 (es) 2018-02-28
EP2801612B1 (en) 2017-11-22
US20150037850A1 (en) 2015-02-05
KR20130080944A (ko) 2013-07-16

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