Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/14—Fungi; Culture media therefor
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
  • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
  • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
  • A01N47/44—Guanidine; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
  • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
  • A01N63/36—Penicillium
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D313/00—Heterocyclic compounds containing rings of more than six members having one oxygen atom as the only ring hetero atom
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/14—Fungi; Culture media therefor
  • C12N1/145—Fungi isolates
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
  • C12P7/00—Preparation of oxygen-containing organic compounds
  • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
  • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
  • C12P7/06—Ethanol, i.e. non-beverage
  • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
  • C12R2001/00—Microorganisms ; Processes using microorganisms
  • C12R2001/645—Fungi ; Processes using fungi
  • C12R2001/80—Penicillium

Definitions

• the invention relates to the field of agriculture and ornamental horticulture and can be used in forestry, crop production, in particular in viticulture, to protect plants from pathogens.
• Bordeaux liquid is used to protect plants, which is prepared from copper sulfate (300 g) and hydrated lime (400 g), each of which is dissolved separately in 5 liters of water and slowly mixed, adding hydrated lime to copper sulfate with constant stirring.
• copper inhibits the development of pathogenic fungi
• fresh slaked lime is used to neutralize the acidity of copper sulphate and thereby prevent acid burn of plants.
• hydrated lime interacts with carbon dioxide of the air and transforms into a substance that becomes unsuitable for the preparation of a solution as a fungicide.
• the disadvantage of using Bordeaux liquid is that it contains a pesticide - copper sulfate, which, although it exhibits its fungicidal effect in contact with pathogens, is easily washed off the surface of the leaves and therefore requires spraying after every rain, while the copper falling in the rain into the soil, causes soil fatigue, and with excessive accumulation in the soil causes toxicosis of plants.
• this fungicidal composition is multicomponent in composition, its components, such as wood-destroying fungi and Sosnowski cow juice, are harmful to humans, both upon receipt of these components and when used as part of a fungicidal composition.
• Sosnovsky hogweed juice causes irritation on the skin of a person and leads to severe allergic diseases.
• the basis of the invention is the task of obtaining a fungicidal composition by introducing curvularin in its composition, which is obtained from the vital products of the strain of the fungus Penicillium roseopurpureum, and an aqueous extract of the silicon-containing natural mineral analcime, which, by increasing the fungicidal action, while eliminating the toxicity of the fungicidal composition, provides cheaper and easier on the technology of manufacturing an effective drug that is not harmful to the environment and mammals, in addition the number of treatments plants, as well as fungicidal composition has a long shelf life.
• curvularin is used - the product of the secondary metabolism of the strain of the fungus Penicillium roseopurpureum and analcime, which is synergistic with respect to the fungicidal effect of curvularin;
• a strain of the fungus Penicillium roseopurpureum is cultured on a nutrient medium, which is deposited in the depository of microorganisms of the D.K.
• the culture fluid containing the product of the secondary metabolism of curvularin is separated from the mycelium, and it (culture fluid) is introduced into the aqueous extract of the silicon-containing natural mineral analcime; according to the second embodiment, in the method for producing the fungicidal composition according to the invention, a strain of the fungus Penicillium roseopurpureum is cultured on a nutrient medium, which is deposited in the depository of microorganisms of the D.K.
• the fungicidal composition according to the invention contains a solution of culture fluid containing curvularin, a product of the secondary metabolism of the strain of the fungus Penicillium roseo purpureum, and an aqueous extract of a silicon-containing natural mineral analcime with a particle size of at least 5 nm in an amount of 50-100 mg per 1 liter of water, taken in a ratio of 1: 1 in volume units, according to the second embodiment, the fungicidal composition according to the invention contains curvularin powder - a product of secondary metabolism strain of the fungus Penicillium
• curvularin a product of the secondary metabolism of the strain of the fungus Penicillium roseopurpureum
• curvularin belongs to the class of phenolic compounds, inhibits the development of phytopathogenic microorganisms in the early stages of plant infection.
• the culture fluid containing curvularin or coulvularin in powder form, as a product of the secondary metabolism of the strain of the fungus Penicillium roseopurpureum is environmentally friendly, since the raw materials for the production of culture fluid containing curvularin or curvularin in powder form are non-toxic.
• analcime which is synergistic to curvularin in the fungicidal composition, is explained by the fact that analcime for the most part consists of mobile silicon (40-45)% and contains up to 30 more chemical elements, including and rare-earth metals, while silicon, which is part of analcime, enters the plants, strengthens the skeletal structure of plants, prevents the penetration of pathogenic microorganisms into plant cells and increases their resistance to stress factors, thereby enhancing the action of curvularin as part of the culture fluid, and in powder form.
• curvularin and analcime in the fungicidal composition helps to increase the resistance of plants to abiotic factors and pathogens and, by the mechanism of action, brings these fungicidal compositions closer to the class of systemic fungicides.
• the culture fluid of the strain of the fungus Penicillim roseopurpureum in addition to curvularin, contains biologically active compounds, namely amino acids, carbohydrates, organic acids, which stimulate the growth and development of plant organisms.
• curvularin in powder form in the fungicidal composition ensures a longer storage time.
• an aqueous extract of silicon-containing natural mineral analcime with a particle size of at least 5 nm is due to the best penetration of particles of analcime to the skeletal part of plants.
• the technical result is determined: an increase in the fungicidal action while eliminating the toxicity of the fungicidal composition.
• the processing of plants, for example, grapes with fungicides during the growing season is carried out 15-16 times per season, which causes great harm to the environment.
• the use of the proposed fungicidal composition provides for a significant reduction in the number of plant treatments by increasing their adaptive ability.
• the preparation of the inventive fungicidal compositions is as follows. Preliminarily receive, according to one of the variants of the claimed methods, a culture fluid containing curvularin or curvularin in the form of a powder and they are introduced into the aqueous extract of analcime. Quantitative indicators of the constituent ingredients of the claimed fungicidal compositions selected according to the results of the experiments.
• test culture Botrytis cinerea and Fusarium graminearum was seeded in a Petri dish with agarized Chapek’s medium containing the studied fungicidal compositions in 0.01% concentration and kept at a temperature of 27–28 ° ⁇ for 21 days.
• the fungicides Thanos, Antracol and prototype were used as standards; the reliability of the studies was 95%.
• the research results are shown in the table.
• Table 1 shows the effect of fungicidal compositions on the test culture of micromycetes in example 1.
• the treatment of vegetative grape plants was carried out after the first signs of mildew by the known fungicides and the claimed fungicidal composition containing curvularin powder and an aqueous extract of silicon-containing natural mineral analcime with a particle size of 5 nm, taken in a ratio of 0.01-0.02: 10 - 20 based on dry weight.
• the consumption rate of fungicides is 5-8 l / ha.

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• Life Sciences & Earth Sciences (AREA)
• Engineering & Computer Science (AREA)
• Chemical & Material Sciences (AREA)
• Health & Medical Sciences (AREA)
• Zoology (AREA)
• Organic Chemistry (AREA)
• Wood Science & Technology (AREA)
• Biotechnology (AREA)
• General Health & Medical Sciences (AREA)
• Microbiology (AREA)
• Genetics & Genomics (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Virology (AREA)
• Mycology (AREA)
• General Engineering & Computer Science (AREA)
• Biochemistry (AREA)
• Environmental Sciences (AREA)
• Pest Control & Pesticides (AREA)
• Plant Pathology (AREA)
• Biomedical Technology (AREA)
• Botany (AREA)
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• Tropical Medicine & Parasitology (AREA)
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• Agricultural Chemicals And Associated Chemicals (AREA)

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Citations (2)

• 2012
  • 2012-01-12 UA UAA201200351A patent/UA108203C2/ru unknown
  • 2012-04-04 WO PCT/UA2012/000035 patent/WO2013105909A1/fr not_active Ceased

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