WO2013113822A3 - Piégeurs d'ozone pour expériences d'hybridation d'acide nucléique - Google Patents

Piégeurs d'ozone pour expériences d'hybridation d'acide nucléique Download PDF

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Publication number
WO2013113822A3
WO2013113822A3 PCT/EP2013/051915 EP2013051915W WO2013113822A3 WO 2013113822 A3 WO2013113822 A3 WO 2013113822A3 EP 2013051915 W EP2013051915 W EP 2013051915W WO 2013113822 A3 WO2013113822 A3 WO 2013113822A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid hybridization
hybridization experiments
ozone scavengers
scavengers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2013/051915
Other languages
English (en)
Other versions
WO2013113822A2 (fr
Inventor
Frank Bergmann
Dieter Heindl
Hans-Peter Josel
John Luckey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical F Hoffmann La Roche AG
Publication of WO2013113822A2 publication Critical patent/WO2013113822A2/fr
Publication of WO2013113822A3 publication Critical patent/WO2013113822A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un procédé, un tampon et une trousse pour piéger des espèces réactives de l'oxygène dans des expériences d'hybridation d'acide nucléique.
PCT/EP2013/051915 2012-02-03 2013-01-31 Piégeurs d'ozone pour expériences d'hybridation d'acide nucléique Ceased WO2013113822A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261594414P 2012-02-03 2012-02-03
US61/594,414 2012-02-03

Publications (2)

Publication Number Publication Date
WO2013113822A2 WO2013113822A2 (fr) 2013-08-08
WO2013113822A3 true WO2013113822A3 (fr) 2013-10-10

Family

ID=48326245

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2013/051915 Ceased WO2013113822A2 (fr) 2012-02-03 2013-01-31 Piégeurs d'ozone pour expériences d'hybridation d'acide nucléique

Country Status (1)

Country Link
WO (1) WO2013113822A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113186037B (zh) * 2021-04-23 2022-12-13 山东博弘基因科技有限公司 一种核酸清除剂

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004108966A1 (fr) * 2003-05-30 2004-12-16 Agilent Technologies Inc. Dosages en jeux de ligands a faible degradation des colorants fluorescents et compositions pour leur mise en pratique
WO2012037394A1 (fr) * 2010-09-16 2012-03-22 Ibis Biosciences, Inc. Stabilisation de colorants fluorescents labiles à l'ozone par une thiourée

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004108966A1 (fr) * 2003-05-30 2004-12-16 Agilent Technologies Inc. Dosages en jeux de ligands a faible degradation des colorants fluorescents et compositions pour leur mise en pratique
WO2012037394A1 (fr) * 2010-09-16 2012-03-22 Ibis Biosciences, Inc. Stabilisation de colorants fluorescents labiles à l'ozone par une thiourée

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"Index of /~astuart/CMB_Lab", 22 September 2009 (2009-09-22), Retrieved from the Internet <URL:http://phobos.ramapo.edu/~astuart/CMB_Lab/> [retrieved on 20130613] *
"Search Results", 13 June 2013 (2013-06-13), Retrieved from the Internet <URL:http://www.nimblegen.com/search.html?q=+CGH%2FLOH+> [retrieved on 20130613] *
A. STUART: "Hybridization of Yeast cDNAs to Microarrays", 22 September 2009 (2009-09-22), XP002698814, Retrieved from the Internet <URL:http://phobos.ramapo.edu/~astuart/CMB_Lab/Genisphere_hybridiz_protocol.doc> [retrieved on 20130613] *
CHAN ET AL.: "Development of a Dye-Protecting Solution for Preventing Fluorescent Dye Degradation on DNA microarrays", 15 March 2004 (2004-03-15), XP002698817, Retrieved from the Internet <URL:http://www.chem.agilent.com/library/posters/public/5989_0924en_ozone_abrf.pdf> [retrieved on 20130613] *
J. PAINTER ET AL.: "Metrics for optimizing CGH/LOH array data quality", NIMBLEGENTECHNICAL NOTE FEB 2012, 1 February 2012 (2012-02-01), XP002698816, Retrieved from the Internet <URL:http://www.nimblegen.com/downloads/support/06650007001_NG_CGH-LOH_OptimizingDataQuality_TN_0212.pdf> [retrieved on 20130613] *
LUCKEY ET AL.: "Ozone effects onNimbleGen dual-colorDNA labeling microarraydata quality", NIMBLEGEN TECHNICAL NOTE JANUARY 2012, 21 January 2012 (2012-01-21), XP002698815, Retrieved from the Internet <URL:http://iucontent.iu.edu.sa/scholars/workflow%20development/Ozone%20effects%20on%20NimbleGen%20dual-color%20DNA%20labeling%20microarray%20data%20quality.pdf> [retrieved on 20130613] *

Also Published As

Publication number Publication date
WO2013113822A2 (fr) 2013-08-08

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