Classifications

• • D—TEXTILES; PAPER
  • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
  • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
  • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
  • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/16—Organic compounds
  • C11D3/38—Products with no well-defined composition, e.g. natural products
  • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
  • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase

Definitions

• the present invention concerns combinations of fabric care enzymes, and in particular but not exclusively fabric care enzymes in a fabric treatment
• WO95/02675 discloses detergent compositions comprising cellulases capable of providing improved particulate soil removal and cellulases providing a colour clarification. Disclosed are compositions comprising a first cellulase component having retaining type activity and being capable of particulate soil removal a second cellulase component having multiple domains comprising at least one non-catalytic domain attached to a catalytic domain and being capable of colour clarification.
• the present invention provides a low temperature active enzymatic fabric treatment composition comprising the combination of:
• the present invention provides a low temperature enzymatic fabric treatment process comprising the step of treating a fabric with the
• composition of the first aspect of the invention is a composition of the first aspect of the invention.
• the present invention provides use of an auxiliary care enzyme in combination with a primary care enzyme in the treatment of fabrics for a care benefit.
• care of the fabric is improved using a low level of fabric care enzyme whose performance is synergistically improved at low temperature by the use of an auxiliary care enzyme.
• the total amount of the enzymes of the invention ie. the total amount of the primary care enzyme and the auxiliary care enzyme is 0.01 - 1 .75 mg per Litre of the wash liquor
• Washing machines vary in wash liquor volume from 10 L to 48 L. Accordingly the total amount of enzymes of the invention per dose may be from 0.1 - 84 mg .
• the wash liquor may be around 4 - 5 litres in which case the dose may be 0.04 - 8.75 mg of total enzymes of the invention.
• the amount of detergent composition per dose will itself vary (e.g. 35 ml, 10 ml ) depending on water levels, concentration of e.g. surfactants.
• the primary care enzyme may be 0.003 mg/L of wash liquor.
• the auxiliary care enzyme is therefore present either in equal weight (of enzyme protein) or, more preferably greater amounts than the primary care enzyme.
• the primary care enzyme and the auxiliary care enzyme are present in a weight ratio of enzyme protein in the range of from 1 : 1 to 1 :29, more preferably 1 :1 to 1 :9 more preferably 1 :5, more preferably 1 :1 .9.
• the preferred ranges of the invention exclude 1 :30, 1 :10 and 1 :2.
• the one or more primary care enzymes comprises one or more enzymes of the Glycoside Hydrolase Family 45.
• the one or more primary care enzymes comprises one or cellulolytic enzymes (commonly known as cellulases but not restricted to the class
• the one or more auxiliary care enzymes preferably comprise one or more glycosyl hydrolases of Family 5 and/or Family 7.
• Primary care enzymes means enzymes active at restoring colour to fabrics by removing fuzz and pills from the surface of the fabric.
• care benefit means restoration of colour to or improvement of feel of fabrics by removing fuzz and pills from the surface of the fabric.
• auxiliary care enzymes means any enzyme which is active to hydrolyse cellooligosaccharides.
• “Low temperature active” means active at temperatures of 25 degrees Celcius or lower.
• “Cellooligosaccharides” means any subchain of cellulose which is a reaction product of the hydrolysis cellulose by the primary care enzyme.
• DP means degrees of polymerisation).
• Glycoside Hydrolase Family means any Glycoside Hydrolase Family
• Glycoside Hydrolase Family 5" includes the retaining enzymes of chitosanase (EC 3.2.1 .132); ⁇ -mannosidase (EC 3.2.1 .25); cellulase (EC 3.2.1 .4); glucan ⁇ - 1 ,3-glucosidase (EC 3.2.1 .58); licheninase (EC 3.2.1 .73); glucan endo-1 ,6- ⁇ - glucosidase (EC 3.2.1 .75); mannan endo- » -1 ,4-mannosidase (EC 3.2.1 .78);
• endo- » -1 ,4-xylanase (EC 3.2.1 .8); cellulose ⁇ -1 ,4-cellobiosidase (EC 3.2.1 .91 ); ⁇ - 1 ,3-mannanase (EC 3.2.1 .-); xyloglucan-specific endo- » -1 ,4-glucanase (EC 3.2.1 .151 ); mannan transglycosylase (EC 2.4.1 .-); endo- » -1 ,6-galactanase (EC 3.2.1 .164); endoglycoceramidase (EC 3.2.1 .123); ⁇ -primeverosidase (EC 3.2.1 .8); cellulose ⁇ -1 ,4-cellobiosidase (EC 3.2.1 .91 ); ⁇ - 1 ,3-mannanase (EC 3.2.1 .-); xyloglucan-specific endo- » -1 ,4-glucanase (EC 3.
• Glycoside Hydrolase Family 7 includes endo- » -1 ,4-glucanase (EC 3.2.1 .4); reducing end-acting cellobiohydrolase (EC 3.2.1 .176); chitosanase (EC
• Glycoside Hydrolase Family 45 includes the inverting enzymes of
• the enzymes of the invention may be from bacterial or fungal origin. Chemically modified or protein engineered mutants are included.
• the primary care and/or the auxiliary care enzymes are cellulases.
• Preferred cellulases include cellulases from the genera Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, Acremonium, e.g. the fungal cellulases produced from Humicola insolens, Thielavia terrestris, Myceliophthora thermophila, and Fusarium oxysporum.
• Especially preferred primary care cellulases are the alkaline or neutral cellulases having color care benefits.
• Such preferred enzymes include those having a molecular weight of from 17kDa to 30 kDa, for example the endoglucanases sold under the tradename Biotouch(R) NCD, DCC and DCL (AB Enzymes, Darmstadt, Germany).
• Other preferred commercially available cellulases include
• auxiliary care cellulases include those active to active to hydrolyse cellooligosaccharides and may include EndolaseTM CellucleanTM.
• enzymes could be included in the fabric treatment composition such as proteases, lipases, phospholipases, amylases, pectate lyases, mannases, peroxidases/oxidases.
• psychrophilic enzymes Whilst mesophilic enzymes are preferred, psychrophilic enzymes may be used and are included in the scope of the invention. Such enzymes include the cellulases and xylanase from e.g. Clostridium sp. PXYL1 (G. Akila, T.S.Chandra (2003) FEMS Microbiol. Letters 219, 63-67). Psychrophilic xylanases include E.coli phagemid (Lee et al. 2006b).
• the fabric treatment composition may comprise a laundry/fabric cleaning/care composition and may comprise one or more surfactants and/or optionally other ingredients.
• compositions of the invention may be in dry solid form e.g. powdered, granules or tableted powders or liquid or gel form. It may also be in the form of a solid detergent bar.
• the composition may be a concentrate to be diluted, rehydrated and/or dissolved in a solvent, including water, before use.
• the composition may also be a ready-to-use (in-use) composition.
• the present invention is suitable for use in industrial or domestic fabric wash compositions, fabric conditioning compositions and compositions for both washing and conditioning fabrics (so-called through the wash conditioner compositions).
• the present invention can also be applied to industrial or domestic non-detergent based fabric care compositions, for example spray-on compositions.
• detergent ingredients may be included including surfactants, builders, sequestring agents, hydrotropes, preservatives, complexing agents, polymers, stabilizers, perfumes, optical brighteners, or other ingredients such as e.g. fabric conditioners including clays, foam boosters, suds suppressors (anti-foams), anti- corrosion agents, soil-suspending agents, anti-soil redeposition agents, antimicrobials, tarnish inhibitors, or combinations of one or more thereof, provided that these ingredients are compatible with the enzymes.
• fabric conditioners including clays, foam boosters, suds suppressors (anti-foams), anti- corrosion agents, soil-suspending agents, anti-soil redeposition agents, antimicrobials, tarnish inhibitors, or combinations of one or more thereof, provided that these ingredients are compatible with the enzymes.
• the fabric wash compositions may comprise a fabric wash detergent material selected from non-soap anionic surfactant, nonionic surfactants, soap, amphoteric surfactants, zwitterionic surfactants and mixtures thereof.
• the surfactants may be present in the composition at a level of from 0.1 % to 60% by weight.
• Any enzyme present in a composition may be stabilized using conventional stabilizing agents, e.g., a polyol such as propylene glycol or glycerol, a sugar or sugar alcohol, lactic acid, boric acid, or a boric acid derivative, e.g., an aromatic borate ester, or a phenyl boronic acid derivative such as 4-formylphenyl boronic acid.
• a polyol such as propylene glycol or glycerol
• a sugar or sugar alcohol lactic acid, boric acid, or a boric acid derivative, e.g., an aromatic borate ester, or a phenyl boronic acid derivative such as 4-formylphenyl boronic acid.
• Enzymes concentrations/ratios A,B and C were prepared as follows.
• BCA reagent solution was prepared by mixing BCA reagents A and B in a 50:1 (v/v) ratio. BCA reagent solution was then mixed in a 1 :1 (v/v) ratio with 100 ⁇ aliquots of sample supernatant solutions in sealed microplate wells and incubated at 65-70°C for 40 minutes. Microplates were then allowed to cool to room temperature in a refrigerator, before well seals were carefully removed. Absorbance of reaction products was measured at 540nm in a spectrophotometer. Interference by protein was accounted for by subtracting protein only (no cotton linters) control readings. Total reducing sugar released was calculated using a standard curve of glucose from 0 - 200 ⁇ .
• Results Tables 1 ,2 and 3 show the results from A, B and C (respectively) as described above : synergistic release of reducing sugars from cotton linters by a 1 :1 mix of three different Family 45 EG-V type endoglucanases (Carezyme, Renozyme and Biotouch) and a Family 7 EG-I type endoglucanase (Endolase). The assay was carried out at 25°C. No enzyme control values have been subtracted from all data. Table 1
• Table 4- (corresponds to Figure 4) showing synergistic release of reducing sugars from cotton linters by a 1 :1 mix of two different Family 45 EG-V type endoglucanases (Carezyme and Biotouch) and a Family 5 EG-I type endoglucanase (Celludean).
• the assay was carried out at 25°C. No enzyme control values have been subtracted from all data.
• a 1 :1 mix of the two types of cellulase according to the invention was found to act synergistically in the release of reducing sugars from cotton linters at 25°C.
• the first cellulase component in this mix is a primary care enzyme, being a Family 45 EG-V type endoglucanase.
• the secondary cellulase component is an auxiliary care enzyme with low activity towards cotton linters.
• enzymes e.g. amylase and/or protease 1
• the enzymes of the invention are included at 0.02 wt % .
• the viscosity of the above exemplary composition is 2700 cps at 20 s ⁇ 1 . However, lower viscosity formulations are possible.

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• Chemical & Material Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Engineering & Computer Science (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Microbiology (AREA)
• Biochemistry (AREA)
• Organic Chemistry (AREA)
• Wood Science & Technology (AREA)
• Oil, Petroleum & Natural Gas (AREA)
• General Chemical & Material Sciences (AREA)
• Textile Engineering (AREA)
• Detergent Compositions (AREA)
• Enzymes And Modification Thereof (AREA)

Priority Applications (4)

Applications Claiming Priority (2)

Publications (1)

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Family Applications (1)

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Cited By (3)

Citations (8)

• 2013
  • 2013-05-07 WO PCT/EP2013/059527 patent/WO2013167613A1/en not_active Ceased
  • 2013-05-07 IN IN2257MUN2014 patent/IN2014MN02257A/en unknown
  • 2013-05-07 EP EP13721724.6A patent/EP2847313A1/en not_active Ceased
  • 2013-05-07 AU AU2013258083A patent/AU2013258083A1/en not_active Abandoned
• 2016
  • 2016-01-13 AU AU2016200189A patent/AU2016200189B2/en active Active

Patent Citations (8)

Non-Patent Citations (6)

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