WO2014000075A3 - Sequência de dna e método para modificar a expressão de genes usando promotor tecido-específico de flor ou fruto de plantas de algodão - Google Patents

Sequência de dna e método para modificar a expressão de genes usando promotor tecido-específico de flor ou fruto de plantas de algodão Download PDF

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Publication number
WO2014000075A3
WO2014000075A3 PCT/BR2013/000230 BR2013000230W WO2014000075A3 WO 2014000075 A3 WO2014000075 A3 WO 2014000075A3 BR 2013000230 W BR2013000230 W BR 2013000230W WO 2014000075 A3 WO2014000075 A3 WO 2014000075A3
Authority
WO
WIPO (PCT)
Prior art keywords
fruit
tissue
dna sequence
specific promoter
cotton plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/BR2013/000230
Other languages
English (en)
French (fr)
Other versions
WO2014000075A2 (pt
Inventor
Marcio Alves Ferreira
Sinara ARTICO
Julia LAMBRET FROTTÉ SILVA
Sarah MUNIZ NARDELI
María Fátima GROSSI DE SÁ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA
Universidade Federal do Rio de Janeiro UFRJ
Original Assignee
Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA
Universidade Federal do Rio de Janeiro UFRJ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA, Universidade Federal do Rio de Janeiro UFRJ filed Critical Empresa Brasileira de Pesquisa Agropecuaria EMBRAPA
Publication of WO2014000075A2 publication Critical patent/WO2014000075A2/pt
Publication of WO2014000075A3 publication Critical patent/WO2014000075A3/pt
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Pregnancy & Childbirth (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A presente invenção refere-se a promotores de expressão de genes específicos em plantas. Mais especificamente, a presente invenção proporciona uma sequência de nucleotídeos para a expressão de genes de interesse em flores e frutos de Gossypium hirsutum para produção de plantas geneticamente modificadas que sejam capazes de resistir ao ataque de pragas, tal como bicudo-do-algodoeiro (Anthonomus grandis). Nomeadamente, a presente invenção descreve construções de DNA que contenham os promotores dos genes de interesse, para criação de plantas geneticamente modificadas, bem como um método para modificação da expressão gênica.
PCT/BR2013/000230 2012-06-28 2013-06-27 Composições e métodos para modificar a expressão de genes usando promotor de genes específicos de flor e fruto de plantas Ceased WO2014000075A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRBR1020120159929 2012-06-28
BR102012015992-9A BR102012015992A2 (pt) 2012-06-28 2012-06-28 Composições e métodos para modificar a expressão de genes usando promotor de genes específicos de flor e fruto de plantas

Publications (2)

Publication Number Publication Date
WO2014000075A2 WO2014000075A2 (pt) 2014-01-03
WO2014000075A3 true WO2014000075A3 (pt) 2014-10-16

Family

ID=49783945

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2013/000230 Ceased WO2014000075A2 (pt) 2012-06-28 2013-06-27 Composições e métodos para modificar a expressão de genes usando promotor de genes específicos de flor e fruto de plantas

Country Status (2)

Country Link
BR (1) BR102012015992A2 (pt)
WO (1) WO2014000075A2 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113789309A (zh) * 2021-09-01 2021-12-14 广东省农业科学院果树研究所 一种细胞色素P450酶及其编码基因CsCYP1与应用

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"Isolamento e caracterização de promotores tecido-especifico de flor de Gosssypium hirsutum, para controle do bicudo-do-algodoeiro.", RESUMOS DO 56° CONGRESSO BRASILEIRO DE GENÉTICA, 14 September 2010 (2010-09-14) - 17 September 2010 (2010-09-17), GUARUJA - SP *
ARTICO ET AL.: "Desenvolvimento de ferramentas biotecnológicas para o controle do bicudo-do-algodoeiro: identificação de gene expressos exclusivamente em tecidos florais para clonagem e caracterização preliminar de promotores específicos de flor.", RESUMOS DO 54° CONGRESSO BRASILEIRO DE GENÉTICA, 16 September 2008 (2008-09-16) - 19 September 2008 (2008-09-19), SALVADOR- BA *
ARTICO ET AL.: "Desenvolvimento de novas ferramentas biotecnológicas para o controle mais efetivo do bicudo-do-algodoeiro: identificação de genes expressos predominantemente em tecidos florais de algodão e clonagem de promotores específicos de flor.", RESUMOS DO VII CONGRESSO BRASILEIRO DO ALGODÃO, 15 September 2009 (2009-09-15) - 18 September 2009 (2009-09-18), FOZ DO IGUAçU, PR, BRASIL, pages 285 - 292 *
ARTICO S.: "Desenvolvimento de ferramentas biotecnológicas para o controle do bicudo-do-algodoeiro: Identificação de genes expressos exclusivamente em tecidos florais de algodao para clonagem de promotores especificos de flor", MESTRADO, DEFESA, February 2010 (2010-02-01) *
VIANA ET AL.: "Isolation and functional characterization of a cotton ubiquitination- related promoter and 5' UTR that drives high levels of expression in foot and flower tissues", BMC BIOTECHNOLOGY, vol. 11, 2011, pages 115 *

Also Published As

Publication number Publication date
BR102012015992A2 (pt) 2014-12-23
WO2014000075A2 (pt) 2014-01-03

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