WO2014016706A3 - Détection de dinoflagellés - Google Patents

Détection de dinoflagellés Download PDF

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Publication number
WO2014016706A3
WO2014016706A3 PCT/IB2013/002481 IB2013002481W WO2014016706A3 WO 2014016706 A3 WO2014016706 A3 WO 2014016706A3 IB 2013002481 W IB2013002481 W IB 2013002481W WO 2014016706 A3 WO2014016706 A3 WO 2014016706A3
Authority
WO
WIPO (PCT)
Prior art keywords
dinoflagellates
detection
detecting
sxtg
exclusively
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2013/002481
Other languages
English (en)
Other versions
WO2014016706A2 (fr
Inventor
Russell ORR
Anke STÜKEN
Kjetill Sigurd Jakobsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitetet i Oslo
Original Assignee
Universitetet i Oslo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitetet i Oslo filed Critical Universitetet i Oslo
Publication of WO2014016706A2 publication Critical patent/WO2014016706A2/fr
Publication of WO2014016706A3 publication Critical patent/WO2014016706A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne une technologie associée à la détection de dinoflagellés produisant de la saxitoxine et en particulier, mais sans y être limitée, des procédés et des compositions pour la détection de dinoflagellés par l'amplification du gène sxtG.
PCT/IB2013/002481 2012-05-16 2013-05-14 Détection de dinoflagellés Ceased WO2014016706A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261647573P 2012-05-16 2012-05-16
US61/647,573 2012-05-16

Publications (2)

Publication Number Publication Date
WO2014016706A2 WO2014016706A2 (fr) 2014-01-30
WO2014016706A3 true WO2014016706A3 (fr) 2014-04-24

Family

ID=49724615

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2013/002481 Ceased WO2014016706A2 (fr) 2012-05-16 2013-05-14 Détection de dinoflagellés

Country Status (1)

Country Link
WO (1) WO2014016706A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118374634B (zh) * 2024-06-24 2024-09-03 中国科学院海洋研究所 检测链状亚历山大藻的特异性引物和探针及应用、试剂盒和检测方法
CN118389741B (zh) * 2024-06-24 2024-08-27 中国科学院海洋研究所 检测太平洋亚历山大藻的特异性引物和探针及应用、试剂盒和检测方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009129558A1 (fr) * 2008-04-24 2009-10-29 Newsouth Innovations Pty Limited Groupes de gènes codant pour la saxitoxine des cyanobactéries et détection d'organismes cyanotoxiques

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4356270A (en) 1977-11-08 1982-10-26 Genentech, Inc. Recombinant DNA cloning vehicle
US5296348A (en) 1989-05-16 1994-03-22 The Wistar Institute Of Anatomy And Biology Methods for screening monoclonal antibodies for therapeutic use
US6995243B2 (en) 2002-08-13 2006-02-07 New York University Antibodies that recognize and bind phosphorylated human glucocorticoid receptor and methods of using same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009129558A1 (fr) * 2008-04-24 2009-10-29 Newsouth Innovations Pty Limited Groupes de gènes codant pour la saxitoxine des cyanobactéries et détection d'organismes cyanotoxiques

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
AMANDA J FOSS ET AL: "Characterization of paralytic shellfish toxins from Lyngbya wollei dominated mats collected from two Florida springs", HARMFUL ALGAE, ELSEVIER, AMSTERDAM, NL, vol. 16, 8 February 2012 (2012-02-08), pages 98 - 107, XP028472965, ISSN: 1568-9883, [retrieved on 20120217], DOI: 10.1016/J.HAL.2012.02.004 *
ANKE STÜKEN ET AL: "Discovery of Nuclear-Encoded Genes for the Neurotoxin Saxitoxin in Dinoflagellates", PLOS ONE, vol. 6, no. 5, 18 May 2011 (2011-05-18), pages e20096 - e20096, XP055103523, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0020096 *
DATABASE EMBL [Online] 12 July 2007 (2007-07-12), "G772P514FF18.T0 Culex pipiens quinquefasciatus Bacteria Infected Library Culex pipiens quinquefasciatus cDNA, mRNA sequence.", XP002720736, retrieved from EBI accession no. EM_EST:EV315883 Database accession no. EV315883 *
DATABASE EMBL [Online] 12 September 2009 (2009-09-12), "Mus musculus NOD/ShiLtJ GSS, clone CH29-191C12 3' sequence", XP002720733, retrieved from EBI accession no. EM_GSS:FR342681 Database accession no. FR342681 *
DATABASE Geneseq [Online] 16 December 1998 (1998-12-16), "ERAB protein coding sequence.", XP002720735, retrieved from EBI accession no. GSN:AAV60576 Database accession no. AAV60576 *
DATABASE Geneseq [Online] 3 June 2004 (2004-06-03), "NEPHA gene transcriptional control region YY1 binding site.", XP002720734, retrieved from EBI accession no. GSN:ADM76355 Database accession no. ADM76355 *
R. J. S. ORR ET AL: "Evolutionary Acquisition and Loss of Saxitoxin Biosynthesis in Dinoflagellates: the Second "Core" Gene, sxtG", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 79, no. 7, 18 January 2013 (2013-01-18), pages 2128 - 2136, XP055103259, ISSN: 0099-2240, DOI: 10.1128/AEM.03279-12 *
RALF KELLMANN ET AL: "Biosynthesis and Molecular Genetics of Polyketides in Marine Dinoflagellates", MARINE DRUGS, vol. 8, no. 4, 31 March 2010 (2010-03-31), pages 1011 - 1048, XP055103260, ISSN: 1660-3397, DOI: 10.3390/md8041011 *
S. A. MURRAY ET AL: "sxtA-Based Quantitative Molecular Assay To Identify Saxitoxin-Producing Harmful Algal Blooms in Marine Waters", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 77, no. 19, October 2011 (2011-10-01), pages 7050 - 7057, XP055103261, ISSN: 0099-2240, DOI: 10.1128/AEM.05308-11 *

Also Published As

Publication number Publication date
WO2014016706A2 (fr) 2014-01-30

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