WO2014102761A3 - Cebadores para detección y tipificación de cepas bacterianas productoras de carbapenemasas, kit y método de detección - Google Patents

Cebadores para detección y tipificación de cepas bacterianas productoras de carbapenemasas, kit y método de detección Download PDF

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Publication number
WO2014102761A3
WO2014102761A3 PCT/IB2014/000230 IB2014000230W WO2014102761A3 WO 2014102761 A3 WO2014102761 A3 WO 2014102761A3 IB 2014000230 W IB2014000230 W IB 2014000230W WO 2014102761 A3 WO2014102761 A3 WO 2014102761A3
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WO
WIPO (PCT)
Prior art keywords
detection
carbapenemase
primers
bacterial strains
kit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2014/000230
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English (en)
French (fr)
Other versions
WO2014102761A2 (es
WO2014102761A8 (es
Inventor
Gloria ROYO GARCÍA
Juan Carlos RODRÍGUEZ DÍAZ
Antonio José GALIANA CABRERA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana FISABIO
Original Assignee
Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana FISABIO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US14/758,458 priority Critical patent/US9914980B2/en
Priority to EP18168831.8A priority patent/EP3392346A1/en
Priority to EP18168826.8A priority patent/EP3392350A1/en
Priority to EP14718156.4A priority patent/EP2940152B1/en
Priority to ES14718156.4T priority patent/ES2689308T3/es
Application filed by Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana FISABIO filed Critical Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana FISABIO
Publication of WO2014102761A2 publication Critical patent/WO2014102761A2/es
Publication of WO2014102761A8 publication Critical patent/WO2014102761A8/es
Publication of WO2014102761A3 publication Critical patent/WO2014102761A3/es
Anticipated expiration legal-status Critical
Priority to US15/877,834 priority patent/US20180179583A1/en
Priority to US15/877,851 priority patent/US20180230520A1/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proponen unas parejas de cebadores, su uso para detección de genes de carbapenemasas y cepas bacterianas productoras de carbapenemasas, y un método de diagnóstico empleando dicho conjunto de cebadores. Se propone además, preferiblemente, el uso de un grupo de sondas dentro de cada reacción de amplificación, más preferiblemente PCR multiplex, para la identificación selectiva y detección de genes de carbapenemasas a partir de una muestra biológica. Los cebadores objeto de esta invención pueden ser utilizados para la preparación de un kit para identificación y detección de genes de carbapenemasas, preferiblemente de genes de carbapenemasas contenidos en cepas bacterianas productoras de dichas enzimas.
PCT/IB2014/000230 2012-12-31 2014-02-28 Cebadores para detección y tipificación de cepas bacterianas productoras de carbapenemasas, kit y método de detección Ceased WO2014102761A2 (es)

Priority Applications (7)

Application Number Priority Date Filing Date Title
US14/758,458 US9914980B2 (en) 2012-12-31 2014-02-28 Primers for the detection and typing of carbapenemase-producing bacterial strains, and detection method and kit
EP18168831.8A EP3392346A1 (en) 2012-12-31 2014-02-28 Primers for detection and typing of carbapenemase producing bacterial strains, and detection method and kit
EP18168826.8A EP3392350A1 (en) 2012-12-31 2014-02-28 Primers for detection and typing of carbapenemase producing bacterial strains, and detection method and kit
EP14718156.4A EP2940152B1 (en) 2012-12-31 2014-02-28 Primers for the detection and typing of carbapenemase-producing bacterial strains, and detection method and kit
ES14718156.4T ES2689308T3 (es) 2012-12-31 2014-02-28 Cebadores para detección y tipado de cepas bacterianas productoras de carbapenemasas, método y kit de detección
US15/877,851 US20180230520A1 (en) 2012-12-31 2018-01-23 Primers for the detection and typing of carbapenemase-producing bacterial strains and detection method and kit
US15/877,834 US20180179583A1 (en) 2012-12-31 2018-01-23 Primers for the detection and typing of carbapenemase-producing bacterial strains and detection method and kit

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ESP2012232077 2012-12-31
ESP2010232077 2012-12-31
ES201232077 2012-12-31

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US14/758,458 A-371-Of-International US9914980B2 (en) 2012-12-31 2014-02-28 Primers for the detection and typing of carbapenemase-producing bacterial strains, and detection method and kit
US15/877,851 Division US20180230520A1 (en) 2012-12-31 2018-01-23 Primers for the detection and typing of carbapenemase-producing bacterial strains and detection method and kit
US15/877,834 Division US20180179583A1 (en) 2012-12-31 2018-01-23 Primers for the detection and typing of carbapenemase-producing bacterial strains and detection method and kit

Publications (3)

Publication Number Publication Date
WO2014102761A2 WO2014102761A2 (es) 2014-07-03
WO2014102761A8 WO2014102761A8 (es) 2014-08-28
WO2014102761A3 true WO2014102761A3 (es) 2015-03-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2014/000230 Ceased WO2014102761A2 (es) 2012-12-31 2014-02-28 Cebadores para detección y tipificación de cepas bacterianas productoras de carbapenemasas, kit y método de detección

Country Status (4)

Country Link
US (3) US9914980B2 (es)
EP (3) EP2940152B1 (es)
ES (1) ES2689308T3 (es)
WO (1) WO2014102761A2 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105671148A (zh) * 2016-01-15 2016-06-15 武汉艾米森生命科技有限公司 用于检测耐碳青霉烯类肠杆菌试剂盒与检测方法
CN107460242B (zh) * 2017-08-15 2022-06-10 北京紫萌医药科技有限公司 一种能同时检测多种高耐药基因的检测试剂盒及其应用
CN107828856B (zh) * 2017-11-23 2020-05-26 中国人民解放军南京军区南京总医院 一种pcr-lf技术检测碳青霉烯类耐药基因kpc和ndm的引物组合物及其应用
CN110904199A (zh) * 2019-11-22 2020-03-24 南方医科大学南方医院 检测碳青霉烯酶blaKPC基因的引物、方法及试剂盒
TR2021019090A1 (tr) 2021-12-04 2023-12-21 Trakya Ueniversitesi Rektoerluegue Direk Karbapenemaz Üçlü (Kpc-2, Oxa-48, Ndm-1) PCR Tanı Kiti

Citations (5)

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CN1396271A (zh) * 2001-07-13 2003-02-12 北京三雄高新技术开发有限责任公司 采用基因芯片技术检测耐药基因
WO2007140004A2 (en) * 2006-05-25 2007-12-06 Creighton University Primers for use in detecting metallo-beta-lactamases
US20090163382A1 (en) * 2006-09-29 2009-06-25 Samsung Electronics Co., Ltd. Primer set for amplifying target sequence(s) of antibiotic-resistant bacterial species, probe or probe set specifically hybridizing with target sequence(s) of antibiotic-resistant bacterial species, method of detecting antibiotic-resistant bacterial species using the probe or probe set, and kit for detecting antibiotic-resistant bacterial species
WO2010130882A1 (en) * 2009-05-15 2010-11-18 Licentia Ltd. A method and kit for detecting antibiotic resistant bacteria
WO2011138402A1 (en) * 2010-05-05 2011-11-10 Check-Points Holding B.V. Assays, compositions and methods for detecting drug resistant micro-organisms

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US7842800B2 (en) * 2004-04-02 2010-11-30 Rosetta Genomics Ltd. Bioinformatically detectable group of novel regulatory bacterial and bacterial associated oligonucleotides and uses thereof
ATE534753T1 (de) 2007-04-06 2011-12-15 Becton Dickinson Co Zusammensetzungen und verfahren zur identifikation eines carbapenemase-gens
RU2415932C1 (ru) 2009-10-23 2011-04-10 Закрытое акционерное общество "Научно-производственное предприятие ИММУНОТЕХ" (ЗАО "НПП ИММУНОТЕХ") Способ идентификации генов бета-лактамаз расширенного спектра действия класса а
EP2694685B1 (en) * 2011-04-08 2019-01-09 Azzazy, Hassan, Mohamed, Ei-said Detection of nucleic acids using unmodified gold nanoparticles
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WO2007140004A2 (en) * 2006-05-25 2007-12-06 Creighton University Primers for use in detecting metallo-beta-lactamases
US20090163382A1 (en) * 2006-09-29 2009-06-25 Samsung Electronics Co., Ltd. Primer set for amplifying target sequence(s) of antibiotic-resistant bacterial species, probe or probe set specifically hybridizing with target sequence(s) of antibiotic-resistant bacterial species, method of detecting antibiotic-resistant bacterial species using the probe or probe set, and kit for detecting antibiotic-resistant bacterial species
WO2010130882A1 (en) * 2009-05-15 2010-11-18 Licentia Ltd. A method and kit for detecting antibiotic resistant bacteria
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Also Published As

Publication number Publication date
EP2940152B1 (en) 2018-06-27
US20160122805A1 (en) 2016-05-05
EP3392350A1 (en) 2018-10-24
WO2014102761A2 (es) 2014-07-03
WO2014102761A8 (es) 2014-08-28
EP2940152A2 (en) 2015-11-04
US20180230520A1 (en) 2018-08-16
EP3392346A1 (en) 2018-10-24
US20180179583A1 (en) 2018-06-28
US9914980B2 (en) 2018-03-13
ES2689308T3 (es) 2018-11-13

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