WO2014121802A1 - Test nano-elisa unique innovant pour la détection du vhc, d'anticorps anti-vih et d'hbsag - Google Patents

Test nano-elisa unique innovant pour la détection du vhc, d'anticorps anti-vih et d'hbsag Download PDF

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Publication number
WO2014121802A1
WO2014121802A1 PCT/EG2013/000002 EG2013000002W WO2014121802A1 WO 2014121802 A1 WO2014121802 A1 WO 2014121802A1 EG 2013000002 W EG2013000002 W EG 2013000002W WO 2014121802 A1 WO2014121802 A1 WO 2014121802A1
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WO
WIPO (PCT)
Prior art keywords
elisa
antibodies
nano
igg
polymers
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Ceased
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PCT/EG2013/000002
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English (en)
Inventor
Mohammad Hosam Eldeen ZAGHLOUL
Ibrahim Mohamed EL-SHERBINY
Mahmoud Omar ASHRI
Farid Abd.Elreheim BADRIA
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Individual
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Individual
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Priority to PCT/EG2013/000002 priority Critical patent/WO2014121802A1/fr
Publication of WO2014121802A1 publication Critical patent/WO2014121802A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles

Definitions

  • ELISA IS THE ABBREVIATION OF "ENZYME-LINKED IMMUNOSORBENT ASSAY”.
  • IT IS A USEFUL AND POWERFUL METHOD IN ESTIMATING NG/ML TO PG/ML ORDERED MATERIALS IN THE SOLUTION, SUCH AS SERUM, URINE, SPERM AND CULTURE SUPERNATANT.
  • THE BASIC PRINCIPLE OF AN ELISA IS TO USE AN ENZYME TO DETECT THE BINDING OF AN ANTIGEN (AG) OR AN ANTIBODY (AB).
  • PROCEDURES ARE SIMPLE AND REQUIRE NO EXTRA TRAINING.
  • FIGURE (1) SHOWS THE INVENTED STRATEGY IN ITS COMPLETE FORM; A: ANTIBODY ,B: LABELING REAGENTS ,C: PATIENT'S SAMPLE, D: SWELLABLE NANOP ARTICLES LOADED WITH LARGE NUMBER OF A SPECIFIC ANTIBODY OR MULTI ANTIBODIES ,1 : BINDING SITE ,2: TARGET ANALYTE (ANTIGEN) ,3: SECOND , ANTIBODY OR TARGET ANALYTE ,4: ENZYME ,5: SUBSTRATE ,6: PRODUCT ,7: INCUBATION ,S: WASHING STEP ,9: ADDITION OF LABELING AGENT 10: INCUBATION ,1 1 : WASHING STEP , 12: ADDITION OF SUBSTRATE ,13: INCUBATION 14: READING.
  • AFTERWARDS ENZYME LABELED REAGENTS (SECOND ANTIBODY CONJUGATED TO ENZYME OR TARGET ANALYTE) IS ADDED AND THE WELL PLATE IS INCUBATED AGAIN. WASHING STEP IS REPEATED FOR REMOVAL OF UNBOUND LABELING REAGENTS.
  • PATIENT'S SERUM OR PLASMA SAMPLE IS ADDED, AND DURING THE FIRST INCUBATION STEP, THE SPECIFIC HCV AND/OR HIV 1/2 ANTIBODIES, AS WELL AS HBSAG AND OR ANTIGEN P24 WILL BE CAPTURED INSIDE THE WELLS IF PRESENT. THE MICRO WELLS ARE THEN WASHED TO REMOVE UNBOUND SERUM PROTEINS.
  • POSITIVE RESULT IS INDICATED BY THE DEVELOPMENT OF COLOR AFTER THE ADDITION OF THE STOPPING REAGENT.
  • OPTICAL DENSITY IS MEASURED FOR THE WELLS ON AN ELISA READER WITH A WAVE LENGTH OF 450 NM.

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Nanotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La présente invention concerne une nouvelle technique économique et fiable pour étendre l'utilisation du protocole ELISA conventionnel pour la détection d'un pathogène unique à la détection de multiples pathogènes (tels que VIH, VHC et HBsAg) dans un seul test avec une sensibilité améliorée. Ce résultat a été obtenu grâce à l'incorporation d'un grand nombre d'un type spécifique d'anticorps ou de types multiples d'anticorps dans des nanoparticules gonflables auto-assemblées conçues de façon distincte et/ou d'hydrogel suivie par la fixation des nanoparticules chargées d'anticorps sur la plaque de test ELISA.
PCT/EG2013/000002 2013-02-07 2013-02-07 Test nano-elisa unique innovant pour la détection du vhc, d'anticorps anti-vih et d'hbsag Ceased WO2014121802A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/EG2013/000002 WO2014121802A1 (fr) 2013-02-07 2013-02-07 Test nano-elisa unique innovant pour la détection du vhc, d'anticorps anti-vih et d'hbsag

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EG2013/000002 WO2014121802A1 (fr) 2013-02-07 2013-02-07 Test nano-elisa unique innovant pour la détection du vhc, d'anticorps anti-vih et d'hbsag

Publications (1)

Publication Number Publication Date
WO2014121802A1 true WO2014121802A1 (fr) 2014-08-14

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EG2013/000002 Ceased WO2014121802A1 (fr) 2013-02-07 2013-02-07 Test nano-elisa unique innovant pour la détection du vhc, d'anticorps anti-vih et d'hbsag

Country Status (1)

Country Link
WO (1) WO2014121802A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114088948A (zh) * 2021-11-09 2022-02-25 重庆创芯生物科技有限公司 一种自驱动微流控芯片的制作方法及自驱动微流控芯片

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997009068A2 (fr) * 1995-09-01 1997-03-13 University Of Washington Conjugues moleculaires interactifs
WO2001055704A1 (fr) * 2000-01-31 2001-08-02 Board Of Regents, The University Of Texas System Systeme de transfert d'echantillons de fluides a travers un ensemble de capteurs
WO2005036175A2 (fr) * 2003-09-03 2005-04-21 Receptors Llc Detecteurs employant des recepteurs artificiels combines
WO2005042695A2 (fr) * 2003-07-30 2005-05-12 Integral Molecular, Inc. Lipoparticules comprenant des proteines, et procedes de production et d'utilisation de ces lipoparticules
US20050175702A1 (en) * 2002-06-01 2005-08-11 Muller-Schulte Detlef P. Thermosensitive polymer carriers having a modifiable physical structure for biochemical analysis, diagnosis and therapy
WO2010025190A1 (fr) * 2008-08-26 2010-03-04 Liotta Lance A Dosage immunologique de base à nanoparticules d’hydrogel
EP2237036A1 (fr) * 2007-12-28 2010-10-06 Ortho-clinical Diagnostics Kabushiki Kaisha Procédé de détection et procédé de détermination pour détecter une cible de détection
US20110003392A1 (en) * 2009-06-12 2011-01-06 Washington, University Of System and Method for Magnetically Concentrating and Detecting Biomarkers

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997009068A2 (fr) * 1995-09-01 1997-03-13 University Of Washington Conjugues moleculaires interactifs
WO2001055704A1 (fr) * 2000-01-31 2001-08-02 Board Of Regents, The University Of Texas System Systeme de transfert d'echantillons de fluides a travers un ensemble de capteurs
US20050175702A1 (en) * 2002-06-01 2005-08-11 Muller-Schulte Detlef P. Thermosensitive polymer carriers having a modifiable physical structure for biochemical analysis, diagnosis and therapy
WO2005042695A2 (fr) * 2003-07-30 2005-05-12 Integral Molecular, Inc. Lipoparticules comprenant des proteines, et procedes de production et d'utilisation de ces lipoparticules
WO2005036175A2 (fr) * 2003-09-03 2005-04-21 Receptors Llc Detecteurs employant des recepteurs artificiels combines
EP2237036A1 (fr) * 2007-12-28 2010-10-06 Ortho-clinical Diagnostics Kabushiki Kaisha Procédé de détection et procédé de détermination pour détecter une cible de détection
WO2010025190A1 (fr) * 2008-08-26 2010-03-04 Liotta Lance A Dosage immunologique de base à nanoparticules d’hydrogel
US20110003392A1 (en) * 2009-06-12 2011-01-06 Washington, University Of System and Method for Magnetically Concentrating and Detecting Biomarkers

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
STOLL D ET AL: "Protein microarrays: Applications and future challenges", CURRENT OPINION IN DRUG DISCOVERY AND DEVELOPMENT, CURRENT DRUGS, LONDON, GB, vol. 8, no. 2, 1 January 2005 (2005-01-01), pages 239 - 252, XP008081397, ISSN: 1367-6733 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114088948A (zh) * 2021-11-09 2022-02-25 重庆创芯生物科技有限公司 一种自驱动微流控芯片的制作方法及自驱动微流控芯片

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