WO2014193237A3 - C diff protease - Google Patents

C diff protease Download PDF

Info

Publication number
WO2014193237A3
WO2014193237A3 PCT/NL2014/050349 NL2014050349W WO2014193237A3 WO 2014193237 A3 WO2014193237 A3 WO 2014193237A3 NL 2014050349 W NL2014050349 W NL 2014050349W WO 2014193237 A3 WO2014193237 A3 WO 2014193237A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease
diff
peptide
invention further
clostridium difficile
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/NL2014/050349
Other languages
French (fr)
Other versions
WO2014193237A2 (en
Inventor
Hans Christiaan VAN LEEUWEN
Paul Jacob HENSBERGEN
Jan Wouter Drijfhout
Eduard KUIJPER
Jeroen CORVER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leids Universitair Medisch Centrum LUMC
Original Assignee
Leids Universitair Medisch Centrum LUMC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leids Universitair Medisch Centrum LUMC filed Critical Leids Universitair Medisch Centrum LUMC
Publication of WO2014193237A2 publication Critical patent/WO2014193237A2/en
Publication of WO2014193237A3 publication Critical patent/WO2014193237A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a peptide comprising the amino acid sequence A/P- A/P-V/P/I/C/T-P as a substrate for the protease CD2830 of Clostridium difficile. The invention further relates to kit or device comprising the peptide for determining the presence of Clostridium difficile in a sample and for identifying an agent that is capable of modulating protease activity of CD2830. The invention further relates to a composition, preferably a pharmaceutical composition, comprising an inhibitor of the protease activity of CD2830.
PCT/NL2014/050349 2013-05-30 2014-05-30 C diff protease Ceased WO2014193237A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP13169930 2013-05-30
EP13169930.8 2013-05-30
EP13197876.9 2013-12-17
EP13197876 2013-12-17

Publications (2)

Publication Number Publication Date
WO2014193237A2 WO2014193237A2 (en) 2014-12-04
WO2014193237A3 true WO2014193237A3 (en) 2015-03-19

Family

ID=50928203

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2014/050349 Ceased WO2014193237A2 (en) 2013-05-30 2014-05-30 C diff protease

Country Status (1)

Country Link
WO (1) WO2014193237A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060121544A1 (en) * 1997-09-15 2006-06-08 Annegret Boge Molecular modification assays
WO2009010765A2 (en) * 2007-07-18 2009-01-22 Novacta Biosystems Limited Lantibiotic-based compounds having antimicrobial activity
US20100291100A1 (en) * 2009-03-27 2010-11-18 Gojo Industries, Inc. Compositions And Methods For Screening And Using Compounds Antagonizing Spore-Surface Interactions

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1254383A (en) 1997-05-01 2000-05-24 美国3M公司 Fluorogenic protease substrates based on dye-dimerization
US20020076741A1 (en) 2000-02-11 2002-06-20 Tencza Sarah Burroughs Peptide biosensors for anthrax protease

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060121544A1 (en) * 1997-09-15 2006-06-08 Annegret Boge Molecular modification assays
WO2009010765A2 (en) * 2007-07-18 2009-01-22 Novacta Biosystems Limited Lantibiotic-based compounds having antimicrobial activity
US20100291100A1 (en) * 2009-03-27 2010-11-18 Gojo Industries, Inc. Compositions And Methods For Screening And Using Compounds Antagonizing Spore-Surface Interactions

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JACQUOT ET AL: "High affinity Grb2-SH3 domain ligand incorporating C<b>eta-substituted prolines in a Sos-derived decapeptide", BIOORGANIC & MEDICINAL CHEMISTRY, PERGAMON, GB, vol. 15, no. 3, 19 January 2007 (2007-01-19), pages 1439 - 1447, XP005823084, ISSN: 0968-0896, DOI: 10.1016/J.BMC.2006.11.002 *
M. VIDAL ET AL: "Design of Peptoid Analogue Dimers and Measure of Their Affinity for Grb2 SH3 Domains +", BIOCHEMISTRY, vol. 43, no. 23, 1 June 2004 (2004-06-01), pages 7336 - 7344, XP055087222, ISSN: 0006-2960, DOI: 10.1021/bi030252n *

Also Published As

Publication number Publication date
WO2014193237A2 (en) 2014-12-04

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