WO2015028811A3 - Methods for detecting proteins or binding partners for proteins - Google Patents

Methods for detecting proteins or binding partners for proteins Download PDF

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Publication number
WO2015028811A3
WO2015028811A3 PCT/GB2014/052620 GB2014052620W WO2015028811A3 WO 2015028811 A3 WO2015028811 A3 WO 2015028811A3 GB 2014052620 W GB2014052620 W GB 2014052620W WO 2015028811 A3 WO2015028811 A3 WO 2015028811A3
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
protein
immobilised
detecting
analyte
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2014/052620
Other languages
French (fr)
Other versions
WO2015028811A2 (en
Inventor
Jonathan Cooper
Julien Reboud
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Glasgow
Original Assignee
University of Glasgow
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Glasgow filed Critical University of Glasgow
Publication of WO2015028811A2 publication Critical patent/WO2015028811A2/en
Publication of WO2015028811A3 publication Critical patent/WO2015028811A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/726—Devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/725—Haemoglobin using peroxidative activity

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Inorganic Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Provided is a method for the detection of a protein, such as haemoglobin or a metalloprotein, in a test sample, such as faecal sample, the method comprising the steps of: (i) exposing the sample to a surface having a binding moiety and permitting the binding moiety to specifically immobilise the protein in the sample; (ii) permitting the immobilised protein to catalyse a reaction of a first reagent with a second reagent; and (iii) detecting, such as electrochemically detecting, the reaction of the first reagent with the second reagent. The binding moiety may be provided on the surface of an electrode. Also provided is a method for detecting an analyte in a sample, which analyte is a binding partner for a protein, the method comprising the steps of: (i) providing a protein that is immobilised, such as specifically immobilised, to a surface; (ii) exposing the immobilised protein to the analyte in the sample, thereby to bind the analyte to the immobilised protein; (iii) exposing the immobilised protein to a first reagent and a second reagent; and (iv) detecting, such as electrochemically detecting, the reaction of first reagent with the second reagent, wherein the immobilised protein is a catalyst for the reaction of the first reagent with the second reagent in the absence of the analyte, and the binding of the analyte to the immobilised protein alters the catalytic activity of the protein for the reaction of the first reagent with the second reagent. The binding moiety can be an antibody. The first reagent can be tetramethylbenzidine and the second reagent can be hydrogen peroxide.
PCT/GB2014/052620 2013-08-30 2014-08-29 Methods for detecting proteins Ceased WO2015028811A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB201315532A GB201315532D0 (en) 2013-08-30 2013-08-30 Methods for detecting proteins
GB1315532.0 2013-08-30

Publications (2)

Publication Number Publication Date
WO2015028811A2 WO2015028811A2 (en) 2015-03-05
WO2015028811A3 true WO2015028811A3 (en) 2015-04-23

Family

ID=49397115

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2014/052620 Ceased WO2015028811A2 (en) 2013-08-30 2014-08-29 Methods for detecting proteins

Country Status (2)

Country Link
GB (1) GB201315532D0 (en)
WO (1) WO2015028811A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20200016315A1 (en) * 2017-03-21 2020-01-16 Syddansk Universitet Purification of blood samples with hemolysis
CN110244050B (en) * 2019-06-11 2022-12-23 中央民族大学 A cell lysis in-situ optical sensing detection chip and its preparation and use method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5506114A (en) * 1992-02-07 1996-04-09 Osborn Laboratories Methods and kits for detecting the presence or concentration of biological analytes
WO2010055306A1 (en) * 2008-11-13 2010-05-20 Mode Diagnostics Limited Electrode, electrochemical sensor and apparatus, and methods for operating the same
WO2011023949A2 (en) * 2009-08-24 2011-03-03 The University Court Of The University Of Glasgow Fluidics apparatus and fluipics substrate

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5506114A (en) * 1992-02-07 1996-04-09 Osborn Laboratories Methods and kits for detecting the presence or concentration of biological analytes
WO2010055306A1 (en) * 2008-11-13 2010-05-20 Mode Diagnostics Limited Electrode, electrochemical sensor and apparatus, and methods for operating the same
WO2011023949A2 (en) * 2009-08-24 2011-03-03 The University Court Of The University Of Glasgow Fluidics apparatus and fluipics substrate

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DONG-MIN KIM ET AL: "Disposable Amperometric Glycated Hemoglobin Sensor for the Finger Prick Blood Test", ANALYTICAL CHEMISTRY, vol. 85, no. 13, 2 July 2013 (2013-07-02), pages 6536 - 6543, XP055151954, ISSN: 0003-2700, DOI: 10.1021/ac401411y *
GREGOIRE HERZOG ET AL: "Electrochemical strategies for the label-free detection of amino acids, peptides and proteins", THE ANALYST, vol. 132, no. 7, 1 July 2007 (2007-07-01), pages 615 - 32, XP055151993, ISSN: 0003-2654, DOI: 10.1039/b701472d *

Also Published As

Publication number Publication date
WO2015028811A2 (en) 2015-03-05
GB201315532D0 (en) 2013-10-16

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