WO2015181008A1 - Mélanges ternaires comprenant des biopesticides et des fongicides chimiques et des insecticides chimiques - Google Patents

Mélanges ternaires comprenant des biopesticides et des fongicides chimiques et des insecticides chimiques Download PDF

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WO2015181008A1
WO2015181008A1 PCT/EP2015/061064 EP2015061064W WO2015181008A1 WO 2015181008 A1 WO2015181008 A1 WO 2015181008A1 EP 2015061064 W EP2015061064 W EP 2015061064W WO 2015181008 A1 WO2015181008 A1 WO 2015181008A1
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leguminosarum
plant
plantarum
amyloliquefaciens
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Ronald Wilhelm
Thorsten Jabs
Nenad FILAJDIC
Giridhar RANUVA
Eda REINOT
Charles MCFATRICH
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BASF SE
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BASF SE
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action

Definitions

  • the present invention relates to mixtures comprising, as active components at least one biopesticide and at least one chemical fungicide and at least one chemical insecticide as defined herein and their use in crop protection especially as treatment of plant propagation materials.
  • a pesticide is generally a chemical or biological agent (such as a virus, bacterium, antimicrobial or disinfectant) that through its effect deters, incapacitates, kills or otherwise discourages pests.
  • Target pests can include insects, plant pathogens, weeds, mollusks, birds, mammals, fish, nematodes (roundworms), and microbes that destroy property, cause nuisance, spread disease or are vectors for disease.
  • pesticide includes also plant growth regulators that alter the expected growth, flowering, or reproduction rate of plants; defoliants that cause leaves or other foliage to drop from a plant, usually to facilitate harvest; desiccants that promote drying of living tissues, such as unwanted plant tops; plant activators that activate plant physiology for defense of against certain pests; safeners that reduce unwanted herbicidal action of pesticides on crop plants; and plant growth promoters that affect plant physiology e.g. to increase plant growth, biomass, yield or any other quality parameter of the harvestable goods of a crop plant.
  • Biopesticides have been defined as a form of pesticides based on micro-organisms
  • Biopesticides fall into two major classes, microbial and biochemical pesticides:
  • Microbial pesticides consist of bacteria, fungi or viruses (and often include the metabolites that bacteria and fungi produce). Entomopathogenic nematodes are also classified as microbial pesticides, even though they are multi-cellular.
  • Biochemical pesticides are naturally occurring substances or or structurally-similar and functionally identical to a naturally-occurring substance and extracts from biological sources that control pests or provide other crop protection uses as defined below, but have non-toxic mode of actions (such as growth or developmental regulation, attractents, repellents or defence activators (e.g. induced resistance) and are relatively non-toxic to mammals.
  • biochemical pesticides include, but are not limited to semiochemicals (insect pheromones and kairomones), natural plant and insect regulators, naturally-occurring repellents and attractants, and proteins (e.g. enzymes).
  • Inoculants also known as microbial or soil inoculants are agricultural amendments that use beneficial endophytes (microbes) to promote plant health.
  • the rhizobacteria commonly applied as inoculants include nitrogen-fixers and phosphate-solubilisers which enhance the availability of the macronutrients nitrogen and phosphorus to the host plant.
  • Such bacteria are commonly referred to as plant growth promoting rhizobacteria (PGPR).
  • Biopesticides for use against crop diseases have already established themselves on a variety of crops. For example, biopesticides already play an important role in controlling downy mildew diseases. Their benefits include: a 0-Day Pre-Harvest Interval, the ability to use under moderate to severe disease pressure, and the ability to use in mixture or in a rotational program with other registered pesticides.
  • Biopesticidal seed treatments are e.g. used to control soil borne fungal pathogens that cause seed rots, damping-off, root rot and seedling blights. They can also be used to control internal seed borne fungal pathogens as well as fungal pathogens that are on the surface of the seed.
  • Many biopesticidal products also show capacities to stimulate plant host defenses and other physiological processes that can make treated crops more resistant to a variety of biotic and abiotic stresses or can regulate plant growth. Many biopesticidal products also show capacities to stimulate plant health, plant growth and/or yield enhancing activity.
  • plant health is to be understood to denote a condition of the plant and/or its products which is determined by several indicators alone or in combination with each other such as yield (e. g. increased biomass and/or increased content of valuable ingredients), plant vigor (e. g. improved plant growth and/or greener leaves ("greening effect")), quality (e. g. improved content or composition of certain ingredients) and tolerance to abiotic and/or biotic stress.
  • yield e. g. increased biomass and/or increased content of valuable ingredients
  • plant vigor e. g. improved plant growth and/or greener leaves ("greening effect")
  • quality e. g. improved content or composition of certain ingredients
  • tolerance to abiotic and/or biotic stress e. g. improved content or composition of certain ingredients
  • biopesticides under certain conditions can also have disadvantages such as high specificity: which may require an exact identification of the pest/pathogen and the use of multiple products to be used, slow speed of action (thus making them unsuitable if a pest outbreak is an immediate threat to a crop), variable efficacy due to the influences of various biotic and abiotic factors (since biopesticides are usually living organisms, which bring about pest/pathogen control by multiplying within the target insect pest/pathogen) and resistance development.
  • It is an object of the present invention overcome the abovementioned disadvantages and to provide, with a view to effective resistance management and effective control of phytopatho- genie harmful fungi, insects or other pests or to effective plant growth promotion, plant stress tolerance and/or seed germination, at application rates which are as low as possible, compositions which, at a reduced total amount of active compounds applied, have improved activity against the harmful fungi, insects or other pests or improved plant growth promoting, stress tolerance inducing and/or improved seeed germination activity (synergistic mixtures) and a broadened activity spectrum, in particular for certain indications.
  • the present invention relates to mixtures comprising, as active components,
  • IA other microbial and biochemical pesticides selected from: Bacillus amyloliquefaciens, B. amyloliquefaciens ssp. plantarum, B. pumilus, B. subtilis, B. simplex, B. megaterium, B. altitudinis, B. mojavensis, B. mycoides, B.
  • Nitrogen-fixing bacteria selected from: Bradyrhizobium japonicum, B. elkanii,
  • Bradyrhizobium spp. Bradyrhizobium sp. (Arachis), Bradyrhizobium sp. (Vigna), B.
  • inhibitors of complex III at Q 0 site selected from: pyraclostrobin, azoxystrobin,
  • picoxystrobin trifloxystrobin, dimoxystrobin, enestroburin, fenaminstrobin, fluoxastrobin, kresoxim-methyl, mandestrobine, metominostrobin, orysastrobin, pyrametostrobin, pyraoxystrobin;
  • Basidiomycetes-specific inhibitors of complex II selected from: carboxin, benodanil, fenfuram, flutolanil, furametpyr, mepronil, oxycarboxin, thifluzamide;
  • E) fungicidal azole compounds selected from: ipconazole, difenoconazole, prothioconazole, prochloraz, triticonazole, flutriafol, cyproconazole, diniconazole, diniconazole-M, fluquinconazole, flusilazole, hexaconazole, imazalil, imibenconazole, metconazole, myclobutanil, simeconazole, tebuconazole, triadimenol, uniconazole, thiabendazole;
  • Oomycetes fungicides selected from: oxathiapiprolin, valifenalate, metalaxyl, mefenoxam, ethaboxam, dimethomorph, zoxamide, flumorph, mandipropamid, pyrimorph,
  • MAP / histidine kinase inhibitor fludioxonil
  • benzimidazole compounds selected from: thiophanate-methyl, carbendazim;
  • dithiocarbamate compounds selected from: thiram, ziram;
  • K) lepidopteran-specific ryanodine receptor inhibitors selected from: chlorantraniliprole and flubendiamide;
  • N) systemically-active neonicotinoid compounds clothianidin, imidacloprid, thiamethoxam, dinotefuran, acetamiprid, flupyradifurone, thiacloprid, triflumezopyrim, nitenpyram;
  • Acetylcholinesterase inhibitors sulfoxaflor, acephate, chlorpyrifos, thiodicarb, abamectin, spinosad.
  • the other microbial and biochemical pesticides IA are considered biopesticides that do not belong to the nitrogen-fixing bacteria IB and have some kind of pesticidal activity.
  • the mixtures comprise at least one biopesticide I and at least one chemical fungicide II and at least one chemical insecticide III in a synergistically effective amount. According to another embodiment, the mixtures comprise one biopoesticide I and one chemical fungicide II and one chemical insecticide III in a synergistically effective amount.
  • the invention also relates to a method for controlling phytopathogenic harmful fungi, insects, nematodes or other pests using mixtures of at least one biopesticide I and at least one chemical fungicide II and at least one chemical insecticide III and to the use of biopesticides I, chemical fungicides II and chemical insecticides III for preparing such mixtures, and to compositions comprising these mixtures and plant propagation materials, in particular seeds, comprising these mixtures respectively coated with such mixtures.
  • the time between the applications may vary e.g. between 2 hours and 7 days. Also a broader range is possible ranging from 0.25 hour to 30 days, preferably from 0.5 hour to 14 days, particularly from 1 hour to 7 days or from 1.5 hours to 5 days, even more preferred from 2 hours to 1 day.
  • the biopesticide I is applied as last treatment.
  • Component 1 in the mixtures comprise biopesticides I which can be separated into the microbial pesticides which comprise bacterial and fungal microorganisms and biochemical pesticides selected from abscisic acid, jasmonic acid, its salts and derivatives thereof, cis- jasmone, methyl jasmonate; and harpin protein.
  • biopesticides I which can be separated into the microbial pesticides which comprise bacterial and fungal microorganisms and biochemical pesticides selected from abscisic acid, jasmonic acid, its salts and derivatives thereof, cis- jasmone, methyl jasmonate; and harpin protein.
  • component 1 ) in the mixtures embraces not only the isolated, pure cultures of the biopesticide I as defined herein, but also its cell-free extract having pesticidal activity, preferably an alcohol- or ketone-based extract, its suspensions in a whole broth culture or as a metabolite-containing supernatant or a purified metabolite obtained from a whole broth culture of the microorganism or microorganism strain.
  • component 1 ) in the mixtures embraces not only the isolated, pure cultures of the biopesticide I as defined herein, but also its cell-free extract having pesticidal activity, preferably an alcohol- or ketone-based extract, its suspensions in a whole broth culture or as a metabolite-containing supernatant or a purified metabolite obtained from a whole broth culture of the microorganism or microorganism strain.
  • Jasmonic acid its salts (jasmonates) or derivatives include without limitation potassium, sodium, lithium, ammonium, dimethylammonium, isopropylammonium, diolammonium and diethtriethanolammonium jasmonate; and also jasmonic acid methyl ester, jasmonic acid amide, jasmonic acid methylamide, jasmonic acid-L-amino acid (amide-linked) conjugates (e. g.
  • whole culture broth refers to a liquid culture of a microorganism containing vegetative cells and/or spores suspended in the culture medium and optionally metabolites produced by the respective microorganism.
  • cell-free extract refers to an extract of the vegetative cells, spores and/or the whole culture broth of a microorganism comprising cellular metabolites produced by the respective microorganism obtainable by cell disruption methods known in the art such as solvent-based (e. g. organic solvents such as alcohols sometimes in combination with suitable salts), temperature-based, application of shear forces, cell disruption with an ultrasonicator.
  • solvent-based e. g. organic solvents such as alcohols sometimes in combination with suitable salts
  • temperature-based e.g. temperature-based
  • shear forces e.g. cell disruption with an ultrasonicator.
  • the desired extract may be concentrated by conventional concentration techniques such as drying, evaporation, and centrifugation or alike. Certain washing steps using organic solvents and/or water-based media may also be applied to the crude extract preferably prior to use.
  • the term "metabolite” refers to any component, compound, substance or byproduct (including but not limited to small molecule secondary metabolites, polyketides, fatty acid synthase products, non-ribosomal peptides, ribosomal peptides, proteins and enzymes) produced by a microorganism (such as fungi and bacteria, in particular the strains of the invention) that has any beneficial effect as described herein such as pesticidal activity or improvement of plant growth, water use efficiency of the plant, plant health, plant appearance, or the population of beneficial microorganisms in the soil around the plant activity herein.
  • a microorganism such as fungi and bacteria, in particular the strains of the invention
  • component 1 is selected from Bacillus amylolique- faciens, B. amyloliquefaciens ssp. plantarum, B. pumilus, B. subtilis, B. simplex, B. megaterium; Burkholderia spp., Coniothyrium minitans, Paecilomyces lilacinus, Paenibacillus alvei,
  • Penicillium bilaiae Pasteuria nishizawa; cis-jasmone, methyl jasmonate and harpin protein.
  • component 1 is selected from Bacillus amyloliquefaciens ssp. plantarum, B. pumilus, B. subtilis and B. simplex. According to a further embodiment, component 1 ) is selected from Bradyrhizobium japonicum, Bradyrhizobium elkanii and Azospirillum brasilense.
  • the mixtures comprise at least four active cmponents, wherein component 1 ) is selected from Bradyrhizobium japonicum, Bradyrhizobium elkanii, Azospirillum brasilense; Bacillus amyloliquefaciens, B. amyloliquefaciens ssp. plantarum, B. pumilus, B. subtilis, B. simplex, B.
  • component 4) is at least one biochemical pesticide selected from abscisic acid, harpin protein, cis-jasmone and methyl- jasmonate; more preferably cis-jasmone or methyl-jasmonate, in particular methyl jasmonate.
  • isolate refers to a pure microbial culture separated from its natural origin, such an isolate obtained by culturing a single microbial colony.
  • An isolate is a pure culture derived from a heterogeneous, wild population of microorganisms.
  • strain refers to isolate or a group of isolates exhibiting phenotypic and/or genotypic traits belonging to the same lineage, distinct from those of other isolates or strains of the same species.
  • biopesticides I their preparation and their pesticidal activity e. g. against harmful fungi or insects are known (e-Pesticide Manual V 5.2 (ISBN 978 1 901396 85 0) (2008-201 1 );
  • biopesticides I may have fungicidal, bactericidal, viricidal, plant defense activator, insecticidal, acaricidal, molluscidal, pheromone, nematicidal, plant stress reducing, plant growth regulator, plant growth promoting and/or yield enhancing activity.
  • Bacillus amyloliquefaciens strains AP-136 (NRRL B-50614 and B-50330), AP-188 (NRRL B-50615 and B-50331), AP-218 (NRRL B- 50618), AP-219 (NRRL B-50619 and B-50332), and AP-295 (NRRL B-50620 and B-50333) all known from US 8,445,255;
  • B. amyloliquefaciens IT-45 e. g. Rhizocell C from ITHEC, France
  • B. amyloliquefaciens IN937a J. Microbiol. Biotechnol. 17(2), 280-286, 2007; e.
  • B. amyloliquefaciens spp. plantarum D747 (US 20130236522 A1 ; FERM BP-8234; e. g. Double NickelTM 55 WDG or Double NickelTM LC from Certis LLC, USA), B. amyloliquefaciens spp. plantarum FZB24 isolated from plant pathogen-infested soil of a sugar beet field in Brandenburg, Germany (also called SB3615;
  • DSM ID 96-2 J. Plant Dis. Prot. 105, 181-197, 1998; e. g. Taegro® from Novozyme Biologicals, Inc., USA), B. amyloliquefaciens ssp. plantarum FZB42 isolated from plant pathogen-infested soil of a sugar beet field in Brandenburg, Germany (J. Plant Dis. Prot. 105, 181-197, 1998; DSM 23117; e. g. RhizoVital® 42 from AbiTEP GmbH, Berlin, Germany), B. amyloliquefaciens ssp. plantarum GB03 (also called GB03; ATCC SD-1397; Phytopathol.
  • B. amyloliquefaciens ssp. plantarum MBI600 also referred to as 1430 (NRRL B-50595; Int. J. Microbiol. Res. 3(2) (201 1 ), 120-130; US 2012/0149571 A1 ; e. g. Integral®, Subtilex® NG from BASF Corp., USA), B. amyloliquefaciens spp.
  • plantarum TJ1000 also called 1 BE; CA 2471555 A1 ; ATCC BAA-390; e. g. QuickRootsTM from TJ Technologies, Watertown, SD, USA), B. cereus CNCM 1-1562 (US 6,406,690), B. chitinosporus AQ746 isolated from roots in Saskatchewan, Canada (NRRL B-21618; US 5,733,544; AgraQuest now Bayer CropScience LP, USA), B. megaterium strains H491 (NRRL B-50769), M018 (NRRL B- 50770) and J142 (NRRL B-50771 ) all known from US 2014/0051571 A1 from Marrone
  • B. mojavensis AP-209 NRRL B-50616; US 8,445,255
  • B. mycoides AQ726 NRRL B-21664; US 5,906,818; from Bayer Crop Science, Germany
  • B. mycoides strain J e.g. BmJ WG from Certis, USA against potato virus Y
  • B. pumilus GB34 ATCC 700814; e. g. YieldShield® from Gustafson LLC, TX, USA
  • B. pumilus GHA 180 isolated from apple tree rhizosphere in Mexico IDAC 260707-01 ; e. g.
  • B. pumilus KFP9F (NRRL B-50754; WO 2014/029697; e. g. BAC-UP or FUSION-P from BASF Agricultural Specialities (Pty) Ltd., South Africa), B. pumilus INR-7 otherwise referred to as BU-F22 and BU- F33 (NRRL B-50185, NRRL B-50153; US 8,445,255), B. pumilus QST 2808 (NRRL B-30087; e. g.
  • B. solisalsi AP-217 (NRRL B-50617; US 8,445,255), B. subtilis CX-9060 (Federal Register 77(7), 1633-1637; by Certis U.S.A., L.L.C.), B. subtilis FB17 also called UD 1022 or UD10-22 isolated from red beet roots in North America (ATCC PTA-1 1857; System. Appl. Microbiol. 27, 372-379, 2004; US 2010/0260735; WO 201 1/109395); B. subtilis GB07 (Phytopathol. 86(1 1 ), S36, 1996; Epic® from Gustafson, Inc., USA), B. subtilis QST-713 isolated from a California peach orchard in 1995 (NRRL
  • B-21661 e. g. Rhapsody®, Serenade® MAX or Serenade® ASO from AgraQuest Inc., USA); Bradyrhizobium sp. (e. g. Vault® from BASF Corp., USA), B. sp. (Arachis) CB1015 presumably originally collected in India (IITA 1006, USDA 3446; from Australian Inoculants Research Group; http://www.qaseeds.com.au/inoculant_applic.php).
  • B. japonicum TA-1 1 (TA1 1 NOD + ) (NRRL B-18466; US 5,021 ,076; Appl. Environ. Microbiol. 56, 2399-2403, 1990; e. g. VAULT® NP, from BASF Corp., USA), B. japonicum strains deposited at USDA known from US 7,262,151 and Appl. Environ. Microbiol.
  • WO 2003/57861 e. g. BioBoost® from Brett Young, Winnipeg, Canada
  • Glomus intraradices e. g. Myc® 4000 from ITHEC, France
  • Glomus intraradices RTI-801 e. g. MYKOS from Xtreme Gardening, USA or RTI Reforestation Technologies International; USA
  • harpin preferably alpha-beta protein (Science 257, 85-88, 1992; e. g. MessengerTM or HARP-N-Tek from Plant Health Care pic, U.K.)
  • Mesorhizobium ciceri CC1 collected in Israel from Cicer arietinum nodules (UPM 848, CECT 5549; Can. J. Microbiol. 48, 279-284, 2002; from
  • M. huakuii HN3015 isolated from Astralagus sinicus in a rice-growing field of Southern China (World J. Microbiol. Biotechn. 23(6), 845-851 , 2007, ISSN 0959-3993), M. /of CC829 isolated from L. ulginosus nodules in USA (NZP 2012; commerical inoculant for Lotus pedunculatus and L. ulginosus in Australia), and M.
  • P. lilacinus BCP2 (NRRL 50756; Acta agriculturae Slovenica, 101 - 2, 263-275, 2013; e. g. PL Gold from BASF Agricultural Specialities (Pty) Ltd., South Africa), Paenibacillus alvei NAS6G6 (WO 2014/029697; NRRL B-50755; e.g. BAC-UP from BASF Agricultural Specialities (Pty) Ltd., South Africa in mixture with Bacillus pumilus KFP9F), Pasteuria sp. ATCC PTA-9643 (WO 2010/085795), Pasteuria sp. Ph3 isolated from turfgrass soil samples collected at the DeBary Golf Course in central Florida (ATCC SD-5832;
  • WO 2012/064527 for control of Hoplolaimus galeatus nematode from Pasteuria Bioscience, Inc. now Syngenta Crop Protection, LLC, USA), Pasteuria sp. Pr3 isolated from soil samples collected in the southeastern United States (ATCC SD-5834; for control of Rotylenchulus reniformis nematode potentially of species P. ramosa; Naviva® ST from Syngenta Crop Protection, LLC, USA), P. nishizawae (WO 2010/80619), P. nishizawae Pn1 (Federal Register 76(22), 5808, February 2, 201 1 ; ATCC SD-5833; e.g. ClarivaTM PN from Syngenta Crop Protection, LLC, USA), P. penetrans (US 5,248,500; Del Monte Corp.), P. ramosa
  • Rhizobium leguminosarum bv. phaseoli e. g. RHIZO-STICK from BASF Corp., USA
  • R. leguminosarum bv. phaseoli RG-B10 USDA 9041 ; from Int. J. Syst. Bacteriol. 46(1 ), 240-244, 1996; Int. J. Syst. Evol. Microbiol. 50, 159-170, 2000; e. g.
  • viciae P1 NP3Cst being a Streptomycin-resis- tant mutant of P1 NP3C isolated from pea root nodules in Breteniere, France (also referred to as 1435; New Phytol. 176, 680-690, 2007; ibid. 179(1 ), 224-235, 2008; e. g. Nodulator® PL Peat Granule from BASF Corp., USA; or Nodulator® XL PL from BASF Agricultural Specialties Ltd., Canada), R. I. bv. viciae RG-P2 also called P2 isolated from pea root nodules in Sakatchewan, Canada (e.
  • RhizUP peat for peas and lentils in Canada from BASF Agricultural Specialties Ltd., Canada R. I. bv. viciae SU303 (e. g. Nodulaid® Group E from BASF Agricultural Specialties Pty Ltd, Australia), R. I. bv. viciae WSM 1455 (e. g. Nodulaid® Group F from BASF Agricultural Specialties Pty Ltd, Australia), R. frop/ ' c/ CC51 1 (Agronomy, N.Z. 36, 4-35, 2006; e. g.
  • Nitragin Nitragin strain collection, The Nitragin Company, Milwaukee, Wisconsin, USA, NRRL or ARSEF (collection of entomopathogenic fungi): ARS Culture Collection of the National Center for Agri- cultural Utilization Research, Agricultural Research Service, U.S.
  • component 1 ) in the mixtures comprises at least one biopesticides I strain from group IA) selected from Bacillus a/f/fuc /n/s 41 KF2b (IA.1), B. amyloliquefaciens AP- 136 (IA.2), B. amyloliquefaciens AP- 188 (IA.3), B. amyloliquefaciens AP- 218 (IA.4), 6. amyloliquefaciens AP-219 (IA.5), 6. amyloliquefaciens AP-295 (IA.6), 6.
  • group IA selected from Bacillus a/f/fuc /n/s 41 KF2b (IA.1), B. amyloliquefaciens AP- 136 (IA.2), B. amyloliquefaciens AP- 188 (IA.3), B. amyloliquefaciens AP- 218
  • amyloliquefaciens ssp. plantarum D747 (IA.7)
  • B. amyloliquefaciens ssp. plantarum FZB24 (IA.8), S. amyloliquefaciens ssp. plantarum FZB42 (IA.9)
  • 6. amyloliquefaciens ssp. plantarum GB03 (IA.10)
  • amyloliquefaciens ssp. plantarum MBI600 (IA.1 1 )
  • 6. amyloliquefaciens ssp. p/anfarum QST-713 (IA.12), 6. amyloliquefaciens ssp. plantarum TJ 1000 (IA.13), 6.
  • megaterium H491 (IA.15), 6. mega fer/um J 142 (IA.16), 6. megaterium M018 (IA.17), 6.
  • mojavensis AP-209 (IA.18), B. mojavensis SR1 1 (IA.19), B. mycoides AQ726 (IA.20), S.
  • mycoides J (IA.21 ), B. pumilus GB34 (IA.22), B. pumilus GHA 180 (IA.23), B. pumilus I NR-7 (IA.24), B. pum/ ' /us KFP9F (IA.25), B. pum/Vus QST 2808 (IA.26), B. simplex ABU 288 (IA.27), B. suW/V/s CX-9060 (IA.28), B. suW/s FB17 (IA.29), B. subtilis GB 7 (IA.30), Burkholderia sp.
  • A396 (IA.31 ), Coniothyrium minitans CON/IW91 -08 (IA.32), Paecilomyces lilacinus 251 (IA.33), Paecilomyces lilacinus BCP2 (IA.34), Paenibacillus alvei NAS6G6 (IA.35), Paenibacillus polymyxa PKB1 (IA.36), Paenibacilllus popilliae 14F-D80 (IA.37), P.
  • Pasteuria sp. ATCC PTA-9643 (IA.42), Pasteuria usgae BL1 (IA.43), Penicillium bilaiae NRRL 50162 (IA.44), P. Ma/ ' ae NRRL 50169 (IA.45), P. bilaiae ATCC 18309 (IA.46), P. bilaiae ATCC 20851 (IA.47), P. bilaiae ATCC 22348 (IA.48), Pseudomonas fluorescens A506 (IA.49), P. fluorescens ATCC 13525 (IA.50), P.
  • fluorescens CHAO IA.51
  • P. fluorescens CL 145A IA.52
  • P. fluorescens NCIB 12089 IA.53
  • P. fluorescens Pf-5 IA.54
  • P. fluorescens WCS374 IA.55
  • P. fluorescens IA.56
  • P. puf/ ' c/a ATCC 202153 IA.57
  • component 1 ) in the mixtures comprises at least one biopesticides I strain from group IA) selected from Bacillus amyloliquefaciens ssp. plantarum D747 (IA.7), B. amyloliquefaciens ssp. plantarum FZB24 (IA.8), B. amyloliquefaciens ssp.
  • group IA selected from Bacillus amyloliquefaciens ssp. plantarum D747 (IA.7), B. amyloliquefaciens ssp. plantarum FZB24 (IA.8), B. amyloliquefaciens ssp.
  • plantarum FZB42 (IA.9)
  • amyloliquefaciens ssp. plantarum QST-713 (IA.12), B. amyloliquefaciens ssp. plantarum TJ 1000 (IA.13), B. pu/ro/us GHA 180 (IA.23), B. pumilus I NR-7 (IA.24), B. pum/Vus QST 2808 (IA.26), B. simplex ABU 288 (IA.27), B. subf/V/s FB17 (IA.29), Burkholderia sp.
  • A396 (IA.31 ), Coniothyrium minitans CON/M/91 -08 (IA.32), Paecilomyces lilacinus 251 (IA.33), Paenibacillus a/ve/ ' NAS6G6 (IA.35), Pasteuria nishizawae Pn1 (IA.39), P. Ma ae ATCC 18309 (IA.46), P. bilaiae ATCC 20851 (IA.47) and P. bilaiae ATCC 22348 (IA.48).
  • component 1 ) in the mixtures comprises at least one biopesticide I from group IA) selected from abscisic acid (IA.58), harpin protein (IA.59), cis- jasmone (IA.60) and methyl-jasmonate (IA.61 ); more preferably selected from harpin protein, cis-jasmone and methyl-jasmonate; in particular methyl jasmonate.
  • group IA selected from abscisic acid (IA.58), harpin protein (IA.59), cis- jasmone (IA.60) and methyl-jasmonate (IA.61 ); more preferably selected from harpin protein, cis-jasmone and methyl-jasmonate; in particular methyl jasmonate.
  • component 1 ) in the mixtures comprises at least one biopesticides I strain from group I B) selected from: Bradyrhizobium elkanii U-1301 (I B.1 1 ), B. e// an/7 U-1302 (I B.12), B. elkanii USDA 3254 (I B.13), B. e// an// USDA 76 (I B.14), B. e//can/7 USDA 94 (I B.15), B. japonicum G49 (I B.18), B. japonicum TA-1 1 (I B.23), B. japonicum USDA 121 (I B.25), B. japonicum USDA 3 (I B.26), B.
  • group I B) selected from: Bradyrhizobium elkanii U-1301 (I B.1 1 ), B. e// an/7 U-1302 (I B.12), B. elkanii USDA 3254 (I B.13), B. e// an/// USDA
  • japonicum USDA 31 (IB.27), B. japonicum USDA 76 (I B.28), Bradyrhizobium sp. (Arachis) CB1015 (I B.29), Bradyrhizobium sp. (Vigna) PNL1 (IB.33), Mesorhizobium sp. WSM 1497 (I B.34), Rhizobium leguminosarum bv. phaseoli RG-B10 (IB.35), f?. leguminosarum bv. phaseoli (I B.36), leguminosarum bv. trifolii 095 (IB.37), f?. leguminosarum bv. trifolii CB782 (IB.38), R. leguminosarum bv. fr/To///// ' CC1099 (IB.39), R.
  • leguminosarum bv. trifolii CC275e (IB.40)
  • R. leguminosarum bv. trifolii CC283b IB.41
  • R. leguminosarum bv. fr/ ' / // RP113-7 (IB.42)
  • R. leguminosarum bv. trifolii ⁇ (IB.43)
  • leguminosarum bv. fr/7o//7 ' WSM1325 (IB.44), R. leguminosarum by. trifolii WSM2304 (IB.45), R leguminosarum bv. v/ ' c/ae P1 NP3Cst (IB.46), R. leguminosarum bv. viciae RG-P2 (IB.47), R. leguminosarum bv. viciae SU303 (IB.48), R. leguminosarum bv. v/ ' c/ae WSM 1455 (IB.49), R.
  • mutant refers a microorganism obtained by direct mutant selection but also includes microorganisms that have been further mutagenized or otherwise manipulated (e. g., via the introduction of a plasmid). Accordingly, embodiments include mutants, variants, and or derivatives of the respective microorganism, both naturally occurring and artificially induced mutants.
  • mutants may be induced by subjecting the microorganism to known mutagens, such as X-ray, UV radiation or N-methyl-nitrosoguanidine, using conventional methods. Subsequent to said treatments a screening for mutant strains showing the desired characteristics may be performed.
  • mutagens such as X-ray, UV radiation or N-methyl-nitrosoguanidine
  • Mutant strains may be obtained by any methods known in the art such as direct mutant selection, chemical mutagenesis or genetic manipulation (e. g., via the introduction of a plasmid).
  • such mutants are obtainable by applying a known mutagen, such as X-ray, UV radiation or N-methyl-nitrosoguanidine. Subsequent to said treatments a screening for mutant strains showing the desired characteristics may be performed.
  • component 1 ) in the mixtures is a microbial pesticide strain of group IA) or a mutant thereof having any pesticidal or other activity as defined herein.
  • the mutant of is obtained by direct mutant selection optionally after being subjected to mutagens.
  • component 1 ) in the mixtures is a nitrogen fixing- bacterium strain of goup IB) as defined herein or a mutant thereof having nitrogen-fixing activity as defined herein.
  • the mutant of is obtained by direct mutant selection optionally after being subjected to mutagens.
  • component 2) in the mixtures comprises at least one chemical fungicide II from the groups A) to I) selected from:
  • inhibitors of complex III at Q 0 site selected from: pyraclostrobin (II.A.1 ), azoxystrobin
  • Basidiomycetes-specific inhibitor of complex II carboxin (II.C.1 );
  • D) ATP production inhibitor silthiofam (II.D.1 );
  • Oomycetes fungicides selected from: oxathiapiprolin (II.F.1 ), valifenalate (II. F.2),
  • Fungicidal MAP / histidine kinase inhibitor fludioxonil (II.G.1 );
  • benzimidazole compounds selected from: thiophanate-methyl (II.H.1 ), carbendazim
  • dithiocarbamate compounds selected from: thiram (II.1.1 ), and ziram (II.I.2).
  • component 2) comprises in the mixtures at least one chemical fungicide II from the groups A), B), E) and F) selected from:
  • A) inhibitor of complex III at Q 0 site pyraclostrobin (II.A.1 );
  • Oomycetes fungicides selected from: oxathiapiprolin (II.F.1), valifenalate (II.F.2).
  • component 3) comprises in the mixtures at least one chemical insecticide III from the groups J) to O) selected from:
  • GABA antagonist compound fipronil (III.J.1 );
  • M pyrethroid sodium channel modulators selected from: tefluthrin (III.M.1 ), bifenthrin
  • N) systemically-active neonicotinoid compounds clothianidin (III.N.1 ), imidacloprid (III.N.2), thiamethoxam (III.N.3), dinotefuran (III.N.4), acetamiprid (III.N.5), flupyradifurone (III.N.6), thiacloprid (III.N.7), triflumezopyrim (III.N.8);
  • Acetylcholinesterase inhibitors sulfoxaflor (III.0.1 ), acephate (III.0.2), chlorpyrifos (III.0.3), thiodicarb (III.0.4), abamectin (III.0.5), spinosad (III.0.6).
  • component 3) in the mixtures comprises at least one chemical insecticide III from the groups J), K) and L) selected from:
  • GABA antagonist compound fipronil (III.J.1 );
  • the present invention relates to mixtures comprising, as active components,
  • biopesticide I selected from: Bacillus amyloliquefaciens ssp. plantarum D747, B. amyloliquefaciens ssp. plantarum FZB24, B. amyloliquefaciens ssp. plantarum FZB42, B. amyloliquefaciens ssp. plantarum MBI600, B. amyloliquefaciens ssp. plantarum QST-713, B. amyloliquefaciens sp. plantarum TJ1000, B. pumilus GHA 180, B. pumilus INR-7, B. pumilus QST 2808, B. simplex ABU 288, B. subtilis FB17, Burkholderia sp. A396,
  • inhibitors of complex III at Q 0 site selected from: pyraclostrobin (II.A.1);
  • fungicidal azole compounds selected from: ipconazole (II.E.1);
  • Oomycetes fungicides selected from: oxathiapiprolin (II.F.1), valifenalate (II.F.2);
  • GABA antagonist compound fipronil (III.J.1);
  • the present invention furthermore relates to agrochemical compositions comprising a mixture of component 1), component 2) and component 3) as described above, and if desired at least one suitable auxiliary.
  • Table 1 Compositions comprising as active components: as component 1) one biopesticide Column Co.1) and as component 2) (in Column Co.2) one chemical fungicide II.

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne des mélanges comprenant comme composants actifs au moins un biopesticide et un fongicide chimique et un insecticide chimique, tels que définis dans la description, et leur utilisation pour la protection des cultures, en particulier comme traitement de matériel de propagation des végétaux.
PCT/EP2015/061064 2014-05-27 2015-05-20 Mélanges ternaires comprenant des biopesticides et des fongicides chimiques et des insecticides chimiques Ceased WO2015181008A1 (fr)

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CN107345215A (zh) * 2017-07-27 2017-11-14 上海万力华生物科技有限公司 一株多粘类芽孢杆菌及其在黄瓜白粉病和霜霉病防治中的应用
US10076119B2 (en) 2012-11-22 2018-09-18 Basf Corporation Pesticidal mixtures
US10251400B2 (en) 2014-05-23 2019-04-09 Basf Se Mixtures comprising a Bacillus strain and a pesticide
CN110692628A (zh) * 2019-11-08 2020-01-17 广西壮族自治区农业科学院 一种毛葡萄霜霉病防治方法以及防治药剂及其制备方法
CN111202079A (zh) * 2020-03-06 2020-05-29 江苏丘陵地区镇江农业科学研究所 一种含有三氟苯嘧啶的农药组合物、制备方法及应用
US10701937B2 (en) 2015-02-11 2020-07-07 Basf Se Pesticidal mixture comprising a pyrazole compound, an insecticide and a fungicide
US10779536B2 (en) 2014-11-07 2020-09-22 Basf Se Pesticidal mixtures
US10905122B2 (en) 2016-03-16 2021-02-02 Basf Se Use of tetrazolinones for combating resistant phytopathogenic fungi on cereals
US11076600B2 (en) 2015-11-30 2021-08-03 Basf Se Mixtures of cis-jasmone and bacillus amyloliquefaciens
US11185078B2 (en) 2016-02-09 2021-11-30 Basf Se Mixtures and compositions comprising Paenibacillus strains or metabolites thereof and other biopesticides
US11241012B2 (en) 2016-03-16 2022-02-08 Basf Se Use of tetrazolinones for combating resistant phytopathogenic fungi on soybean
WO2022128812A1 (fr) 2020-12-17 2022-06-23 Basf Se Compositions de spores, leur production et leurs utilisations
US11425909B2 (en) 2016-03-16 2022-08-30 Basf Se Use of tetrazolinones for combating resistant phytopathogenic fungi on fruits
US12239136B2 (en) 2016-02-09 2025-03-04 Basf Se Mixtures and compositions comprising Paenibacillus strains or fusaricidins and chemical pesticides

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Publication number Priority date Publication date Assignee Title
US10076119B2 (en) 2012-11-22 2018-09-18 Basf Corporation Pesticidal mixtures
US11284623B2 (en) 2012-11-22 2022-03-29 Basf Corporation Pesticidal mixtures
US11083202B2 (en) 2014-05-23 2021-08-10 Basf Se Mixtures comprising a bacillus strain and a pesticide
US10251400B2 (en) 2014-05-23 2019-04-09 Basf Se Mixtures comprising a Bacillus strain and a pesticide
US12302901B2 (en) 2014-11-07 2025-05-20 Basf Se Pesticidal mixtures
US10779536B2 (en) 2014-11-07 2020-09-22 Basf Se Pesticidal mixtures
US10701937B2 (en) 2015-02-11 2020-07-07 Basf Se Pesticidal mixture comprising a pyrazole compound, an insecticide and a fungicide
US11076600B2 (en) 2015-11-30 2021-08-03 Basf Se Mixtures of cis-jasmone and bacillus amyloliquefaciens
US12239136B2 (en) 2016-02-09 2025-03-04 Basf Se Mixtures and compositions comprising Paenibacillus strains or fusaricidins and chemical pesticides
US11185078B2 (en) 2016-02-09 2021-11-30 Basf Se Mixtures and compositions comprising Paenibacillus strains or metabolites thereof and other biopesticides
US11241012B2 (en) 2016-03-16 2022-02-08 Basf Se Use of tetrazolinones for combating resistant phytopathogenic fungi on soybean
US10905122B2 (en) 2016-03-16 2021-02-02 Basf Se Use of tetrazolinones for combating resistant phytopathogenic fungi on cereals
US11425909B2 (en) 2016-03-16 2022-08-30 Basf Se Use of tetrazolinones for combating resistant phytopathogenic fungi on fruits
CN107345215A (zh) * 2017-07-27 2017-11-14 上海万力华生物科技有限公司 一株多粘类芽孢杆菌及其在黄瓜白粉病和霜霉病防治中的应用
CN107345215B (zh) * 2017-07-27 2020-07-03 上海万力华生物科技有限公司 一株多粘类芽孢杆菌及其在黄瓜白粉病和霜霉病防治中的应用
CN110692628A (zh) * 2019-11-08 2020-01-17 广西壮族自治区农业科学院 一种毛葡萄霜霉病防治方法以及防治药剂及其制备方法
CN111202079A (zh) * 2020-03-06 2020-05-29 江苏丘陵地区镇江农业科学研究所 一种含有三氟苯嘧啶的农药组合物、制备方法及应用
WO2022128812A1 (fr) 2020-12-17 2022-06-23 Basf Se Compositions de spores, leur production et leurs utilisations

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