WO2016005985A3 - Méthode pour reprogrammer des cellules - Google Patents

Méthode pour reprogrammer des cellules Download PDF

Info

Publication number
WO2016005985A3
WO2016005985A3 PCT/IL2015/050715 IL2015050715W WO2016005985A3 WO 2016005985 A3 WO2016005985 A3 WO 2016005985A3 IL 2015050715 W IL2015050715 W IL 2015050715W WO 2016005985 A3 WO2016005985 A3 WO 2016005985A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
itsc
eomes
expressing
generating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IL2015/050715
Other languages
English (en)
Other versions
WO2016005985A2 (fr
Inventor
Yossi BUGANIM
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yissum Research Development Co of Hebrew University of Jerusalem
Original Assignee
Yissum Research Development Co of Hebrew University of Jerusalem
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Research Development Co of Hebrew University of Jerusalem filed Critical Yissum Research Development Co of Hebrew University of Jerusalem
Publication of WO2016005985A2 publication Critical patent/WO2016005985A2/fr
Publication of WO2016005985A3 publication Critical patent/WO2016005985A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

La présente invention concerne une méthode de génération d'une cellule souche trophoblastique induite (iTSC) à partir d'une cellule. Par conséquent, l'invention concerne une méthode consistant à exprimer, à l'intérieur de ladite cellule, au moins un facteur de transcription exogène choisi dans le groupe constitué par Gata3, Eomes et Tfap2c, dans des conditions qui permettent la génération d'une iTSC à partir de ladite cellule, ce qui permet de générer l'iTSC à partir de ladite cellule, à condition que la méthode ne consiste pas à exprimer, à l'intérieur de ladite cellule, Eomes, Cdx2, Elf5, cMyc et Klf4. L'invention concerne également des constructions d'acides nucléiques, des cellules isolées et des iTSC.
PCT/IL2015/050715 2014-07-09 2015-07-09 Méthode pour reprogrammer des cellules Ceased WO2016005985A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462022296P 2014-07-09 2014-07-09
US62/022,296 2014-07-09

Publications (2)

Publication Number Publication Date
WO2016005985A2 WO2016005985A2 (fr) 2016-01-14
WO2016005985A3 true WO2016005985A3 (fr) 2016-03-03

Family

ID=53872114

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2015/050715 Ceased WO2016005985A2 (fr) 2014-07-09 2015-07-09 Méthode pour reprogrammer des cellules

Country Status (1)

Country Link
WO (1) WO2016005985A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105624194A (zh) * 2016-02-16 2016-06-01 广东省农业科学院农业生物基因研究中心 一种条件性诱导Cas9表达的猪滋养层细胞系及其建立方法和应用
WO2019180247A1 (fr) * 2018-03-22 2019-09-26 INSERM (Institut National de la Santé et de la Recherche Médicale) Procédé de reprogrammation de cellules somatiques
AU2020381647A1 (en) * 2019-11-13 2022-06-16 Monash University Methods for reprogramming cells
JP7670356B2 (ja) * 2020-07-17 2025-04-30 国立大学法人東北大学 胎盤を構成する細胞への分化能を有する栄養膜幹細胞様細胞、及びその製造方法
WO2022174129A1 (fr) * 2021-02-15 2022-08-18 Arizona Board Of Regents On Behalf Of The University Of Arizona Eomes recombinante qui restaure l'activité anticancéreuse de cellules immunitaires
IL309333A (en) 2021-06-13 2024-02-01 Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd A method for reprogramming cells

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US6774279B2 (en) 1997-05-30 2004-08-10 Carnegie Institution Of Washington Use of FLP recombinase in mice
WO2000017325A1 (fr) 1998-09-23 2000-03-30 Mount Sinai Hospital Preparations de cellules trophoblastiques
US20030232410A1 (en) 2002-03-21 2003-12-18 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
JP2006502748A (ja) 2002-09-05 2006-01-26 カリフォルニア インスティテュート オブ テクノロジー 遺伝子ターゲッティングを誘発するキメラヌクレアーゼの使用方法
US20060014264A1 (en) 2004-07-13 2006-01-19 Stowers Institute For Medical Research Cre/lox system with lox sites having an extended spacer region
EP2960328A1 (fr) 2004-11-04 2015-12-30 Ocata Therapeutics, Inc. Dérivation de cellules souches embryonnaires
US7642091B2 (en) 2005-02-24 2010-01-05 Jau-Nan Lee Human trophoblast stem cells and use thereof
EP2067402A1 (fr) 2007-12-07 2009-06-10 Max Delbrück Centrum für Molekulare Medizin (MDC) Berlin-Buch; Mutagenèse par élément transposable dans les cellules souches spermatogoniales
US8557972B2 (en) 2009-12-21 2013-10-15 University Of Washington Through Its Center For Commercialization Compositions and methods for transfection of RNA and controlled stabilization of transfected RNA
EP3156062A1 (fr) 2010-05-17 2017-04-19 Sangamo BioSciences, Inc. Nouvelles protéines à liaison adn et leurs utilisations
WO2013049389A1 (fr) 2011-09-27 2013-04-04 Yale University Compositions et procédés pour expression transitoire d'arn recombinant
WO2013159103A1 (fr) 2012-04-20 2013-10-24 Whitehead Institute For Biomedical Research Programmation et reprogrammation de cellules
AU2013352156B2 (en) 2012-11-27 2018-12-06 Children's Medical Center Corporation Targeting BCL11A distal regulatory elements for fetal hemoglobin reinduction
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
A. RALSTON ET AL: "Gata3 regulates trophoblast development downstream of Tead4 and in parallel to Cdx2", DEVELOPMENT, vol. 137, no. 3, 1 February 2010 (2010-02-01), pages 395 - 403, XP055216021, ISSN: 0950-1991, DOI: 10.1242/dev.038828 *
CHEN YING ET AL: "Roles of CDX2 and EOMES in human induced trophoblast progenitor cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 431, no. 2, 8 January 2013 (2013-01-08), pages 197 - 202, XP028970948, ISSN: 0006-291X, DOI: 10.1016/J.BBRC.2012.12.135 *
JAMES J L ET AL: "Can we fix it? Evaluating the potential of placental stem cells for the treatment of pregnancy disorders", PLACENTA, vol. 35, no. 2, 2 February 2014 (2014-02-02), pages 77 - 84, XP028608339, ISSN: 0143-4004, DOI: 10.1016/J.PLACENTA.2013.12.010 *
KUBACZKA CAROLINE ET AL: "Derivation and maintenance of murine trophoblast stem cells under defined conditions.", STEM CELL REPORTS 11 FEB 2014, vol. 2, no. 2, 11 February 2014 (2014-02-11), pages 232 - 242, XP055216132, ISSN: 2213-6711 *
P. KUCKENBERG ET AL: "The Transcription Factor TCFAP2C/AP-2 Cooperates with CDX2 To Maintain Trophectoderm Formation", MOLECULAR AND CELLULAR BIOLOGY, vol. 30, no. 13, 1 July 2010 (2010-07-01), pages 3310 - 3320, XP055215974, ISSN: 0270-7306, DOI: 10.1128/MCB.01215-09 *

Also Published As

Publication number Publication date
WO2016005985A2 (fr) 2016-01-14

Similar Documents

Publication Publication Date Title
ZA202310779B (en) Compositions and methods for enhanced gene expression
AU2016250905A8 (en) Generation of muscle-lineage cells from stem cells
EP3161127A4 (fr) Cellules souches limbiques de mammifères en culture, leurs méthodes de production et leurs utilisations
MX2023011785A (es) Metodos y composiciones para produccion de proteina de clara de huevo.
EP4287191A3 (fr) Méthodes et compositions reposant sur le hla et leurs utilisations
SG10201810150UA (en) Compositions and methods for enhanced gene expression in cone cells
WO2016054615A3 (fr) Augmentation ciblée de la production de gènes nucléaires
EP3165609A4 (fr) Cassette d'expression pour la transformation de cellules eucaryotes, procédé pour la transformation de cellules eucaryotes, micro-organisme génétiquement modifié, procédé de production de biocombustibles et/ou d'agents biochimiques et biocombustible et/ou agent biochimique ainsi produits
EP3223831A4 (fr) Procédés de génération de podocytes à partir de cellules souches pluripotentes et cellules obtenues selon ces procédés
EP3188667A4 (fr) Analyse et génération de gènes de procédés à base de cellules souches et appareil
MX375786B (es) Biosintesis microbiana de ergotioneina.
MA39819A (fr) Méthodes et compositions d'immunomodulation
IL266554A (en) Stem cells derived from Schwann cells
EP3216859A4 (fr) Population de cellules à différenciation induite dont les cellules non différenciées ont été retirées, son utilisation, et procédé pour la produire
EP3269003A4 (fr) Conception d'ame intelligents pour piles à combustible de puissance élevée
EP3097411A4 (fr) Biocapteurs salivaires et cellules de biocarburant
EP3108224B8 (fr) Insert à électrode de référence d'un élément électrochimiques d'essai
NZ730124A (en) A method of predicting risk of recurrence of cancer
EP3124602A4 (fr) Milieu de culture pour cellules souches pluripotentes
EP3181686A4 (fr) Milieu de culture et procédé d'enrichissement et de maintien de cellules mères cancérigènes (csc) à l'aide dudit milieu
EP3184487A4 (fr) Générateur d'hydrogène, son procédé de fonctionnement et système de pile à combustible
EP3600365A4 (fr) Méthodes de régulation de l'expression génique dans une cellule
WO2016061240A8 (fr) Éléments promoteurs et régulateurs pour l'expression améliorée de gènes hétérologues dans des cellules hôtes
EP3178784A4 (fr) Appareil de génération d'hydrogène, son procédé de fonctionnement et système de pile à combustible
EP3194573A4 (fr) Cellules souches pluripotentes de mammifère, des procédés pour leur production et leurs utilisations

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15750821

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 15750821

Country of ref document: EP

Kind code of ref document: A2