WO2016071925A3 - Intégration d'un gène de ss-actine pour la vérification de la qualité d'un échantillon dans un kit de diagnostic de vhs-1 et vhs-2 - Google Patents

Intégration d'un gène de ss-actine pour la vérification de la qualité d'un échantillon dans un kit de diagnostic de vhs-1 et vhs-2 Download PDF

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Publication number
WO2016071925A3
WO2016071925A3 PCT/IN2015/000408 IN2015000408W WO2016071925A3 WO 2016071925 A3 WO2016071925 A3 WO 2016071925A3 IN 2015000408 W IN2015000408 W IN 2015000408W WO 2016071925 A3 WO2016071925 A3 WO 2016071925A3
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WIPO (PCT)
Prior art keywords
seq
hsv
nucleotide
nucleotide sequence
beta
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Ceased
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PCT/IN2015/000408
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English (en)
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WO2016071925A2 (fr
Inventor
Amita Jain
Shantanu PRAKASH
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KING GEORGE'S MEDICAL UNIVERSITY
Indian Council of Medical Research
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KING GEORGE'S MEDICAL UNIVERSITY
Indian Council of Medical Research
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Application filed by KING GEORGE'S MEDICAL UNIVERSITY, Indian Council of Medical Research filed Critical KING GEORGE'S MEDICAL UNIVERSITY
Publication of WO2016071925A2 publication Critical patent/WO2016071925A2/fr
Publication of WO2016071925A3 publication Critical patent/WO2016071925A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des amorces et des sondes pour la détection in vitro des virus VHS-1 et virus VHS-2 humains dans un échantillon, comprenant des séquences nucléotidiques choisies dans le groupe comprenant les suivantes ou l'une quelconque de leurs combinaisons : une molécule d'acide nucléique qui code une séquence nucléotidique de SEQ ID NO: 1 et SEQ ID No. 4 ou une partie ou un nucléotide ayant une identité de séquence d'au minimum de 90 % avec ladite SEQ ID NO: 1, laquelle séquence nucléotidique de SEQ ID NO: 1 ou SEQ ID No. 4 représente une amorce sens pour amplifier le virus VHS-1 humain; une molécule d'acide nucléique qui code une séquence nucléotidique de SEQ ID NO: 2 et SEQ ID No. 5 ou une partie ou un nucléotide ayant une identité de séquence d'au minimum de 90 % avec lesdites SEQ ID NO: 2 et SEQ ID No. 5, laquelle séquence nucléotidique de SEQ ID NO: 1 représente amorce antisens pour amplifier VHS-1 humain; et une molécule d'acide nucléique qui code une séquence nucléotidique de SEQ ID NO: 3 et SEQ ID NO. 6 ou une partie ou un nucléotide ayant une identité de séquence d'au minimum de 90 % avec lesdites SEQ ID NO: 3 et SEQ ID No. 6, laquelle séquence nucléotidique de SEQ ID NO: 3 et SEQ ID No. 6 représente des sondes pour détecter HSV-1 et HSV-2 humains. Les séquences nucléotidiques permettent une détection élevée de tous les génotypes de VHS-1 et HSV-2 humains, lesdites séquences de nucléotides permettant la détection de virus présent à faible nombre de copies jusqu'à > 102 UI/ml.
PCT/IN2015/000408 2014-11-05 2015-11-04 Intégration d'un gène de ss-actine pour la vérification de la qualité d'un échantillon dans un kit de diagnostic de vhs-1 et vhs-2 Ceased WO2016071925A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN3198DE2014 2014-11-05
IN3198/DEL/2014 2014-11-05

Publications (2)

Publication Number Publication Date
WO2016071925A2 WO2016071925A2 (fr) 2016-05-12
WO2016071925A3 true WO2016071925A3 (fr) 2016-06-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2015/000408 Ceased WO2016071925A2 (fr) 2014-11-05 2015-11-04 Intégration d'un gène de ss-actine pour la vérification de la qualité d'un échantillon dans un kit de diagnostic de vhs-1 et vhs-2

Country Status (1)

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WO (1) WO2016071925A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201801233UA (en) 2018-02-13 2019-09-27 Delta Electronics Int’L Singapore Pte Ltd Kit and method for detecting hsv1 and hsv2
CN115927743B (zh) * 2022-07-27 2023-11-03 中国疾病预防控制中心病毒病预防控制所 一种用于同时检测hsv-1、hsv-2和vzv的组合物、试剂盒和方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020090610A1 (en) * 2000-06-29 2002-07-11 Hosken Nancy A. Compositions and methods for the diagnosis and treatment of herpes simplex virus infection
US20080318210A1 (en) * 2003-08-27 2008-12-25 Rosetta Genomics Bioinformatically detectable group of novel regulatory viral and viral associated oligonucleotides and uses thereof
KR20120140069A (ko) * 2011-06-20 2012-12-28 주식회사 맥스바이오텍 단순포진 바이러스 1형 및 2형의 진단을 위한 프라이머 및 프로브, 이를 이용한 검사 방법 및 이를 포함하는 검사 키트
WO2013109604A1 (fr) * 2012-01-19 2013-07-25 Alnylam Pharmaceuticals, Inc. Atténuation virale et production de vaccin
WO2014136124A2 (fr) * 2013-03-05 2014-09-12 Indian Council Of Medical Research Trousse d'essai de pcr en temps réel multiplexe pour la détection simultanée du virus de l'hépatite

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060141481A1 (en) 2004-12-27 2006-06-29 Mariani Brian D HSV-1 and HSV-2 primers and probes
EP2561094B1 (fr) 2010-04-21 2017-03-29 Gen-Probe Incorporated Compositions, méthodes et kits permettant de détecter l'acide nucléique du virus de l'herpès simplex

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020090610A1 (en) * 2000-06-29 2002-07-11 Hosken Nancy A. Compositions and methods for the diagnosis and treatment of herpes simplex virus infection
US20080318210A1 (en) * 2003-08-27 2008-12-25 Rosetta Genomics Bioinformatically detectable group of novel regulatory viral and viral associated oligonucleotides and uses thereof
KR20120140069A (ko) * 2011-06-20 2012-12-28 주식회사 맥스바이오텍 단순포진 바이러스 1형 및 2형의 진단을 위한 프라이머 및 프로브, 이를 이용한 검사 방법 및 이를 포함하는 검사 키트
WO2013109604A1 (fr) * 2012-01-19 2013-07-25 Alnylam Pharmaceuticals, Inc. Atténuation virale et production de vaccin
WO2014136124A2 (fr) * 2013-03-05 2014-09-12 Indian Council Of Medical Research Trousse d'essai de pcr en temps réel multiplexe pour la détection simultanée du virus de l'hépatite

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "Iowa Black Dark Quenchers", 11 August 2014 (2014-08-11), XP055266377, Retrieved from the Internet <URL:http://web.archive.org/web/20140811120825/http://eu.idtdna.com/site/Catalog/Modifications/Category/4> [retrieved on 20160418] *
BADDOURA FADY K ET AL: "Latent Epstein-Barr virus infection is an unlikely event in the pathogenesis of immunoproliferative small intestinal disease", CANCER, AMERICAN CANCER SOCIETY, PHILADELPHIA, PA, US, vol. 74, no. 6, 15 September 1994 (1994-09-15), pages 1699 - 1705, XP009158974, ISSN: 0008-543X, [retrieved on 20060628], DOI: 10.1002/1097-0142(19940915)74:6<1699::AID-CNCR2820740610>3.0.CO;2-7 *
DATABASE Geneseq [online] 12 September 2013 (2013-09-12), "Herpes simplex virus type 1 (HSV-1) genome, SEQ: 1.", XP002756653, retrieved from EBI accession no. GSN:BAR54028 Database accession no. BAR54028 *
JOANNIE D SUN ET AL: "Mucosal swabs detect HPV in laryngeal papillomatosis patients but not family members", INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY., vol. 53, no. 2, 1 June 2000 (2000-06-01), NL, pages 95 - 103, XP055266188, ISSN: 0165-5876, DOI: 10.1016/S0165-5876(00)00304-9 *
KESSLER HARALD H: "Design and work-up of a new molecular diagnostic assay based on real-time", METHODS IN MOLECULAR BIOLOGY, HUMANA PRESS, INC, US, vol. 353, 1 January 2007 (2007-01-01), pages 227 - 236, XP009189539, ISSN: 1064-3745 *

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